FC868489 | Citrus Genome Database

Overview


Name	FC868489
Unique Name	FC868489
Type	EST
Organism	Citrus clementina (Clementine)
Sequence length	636

Libraries


Library Name	Type
AbioticL1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Ccv1_Contig9336	contig	Ccv1_Contig9336:1..636.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_ARATH (Ubiquitin OS=Arabidopsis thaliana GN=UBQ1 PE=1 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 6.285e-36
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 147.902 bits (372), Expect = 4.074e-35
Identity = 75/76 (98.68%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQR IFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 4.102e-11
Identity = 35/37 (94.59%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV AKIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_COPCO (Ubiquitin OS=Coprinus congregatus PE=1 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 1.400e-35
Identity = 75/76 (98.68%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 146.747 bits (369), Expect = 9.076e-35
Identity = 74/76 (97.37%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 4.102e-11
Identity = 35/37 (94.59%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV AKIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_CHLRE (Ubiquitin OS=Chlamydomonas reinhardtii GN=UBI1 PE=1 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.829e-35
Identity = 75/76 (98.68%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 146.362 bits (368), Expect = 1.185e-34
Identity = 74/76 (97.37%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTI+NVKAKIQDKEGIPPDQQR IFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_YEAST (Ubiquitin OS=Saccharomyces cerevisiae GN=UBI1 PE=1 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.119e-35
Identity = 74/76 (97.37%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 67.0106 bits (162), Expect = 9.139e-11
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV +KIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_SCHPO (Ubiquitin OS=Schizosaccharomyces pombe GN=ubi1 PE=1 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.119e-35
Identity = 74/76 (97.37%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 67.0106 bits (162), Expect = 9.139e-11
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV +KIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_KLULA (Ubiquitin OS=Kluyveromyces lactis GN=ubi3 PE=1 SV=2)

HSP 1 Score: 148.288 bits (373), Expect = 3.119e-35
Identity = 74/76 (97.37%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 67.0106 bits (162), Expect = 9.139e-11
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV +KIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_CRYNE (Ubiquitin OS=Cryptococcus neoformans GN=UBI1 PE=1 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.119e-35
Identity = 74/76 (97.37%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 67.0106 bits (162), Expect = 9.139e-11
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV +KIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_CANAL (Ubiquitin OS=Candida albicans GN=UBI1 PE=1 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 3.119e-35
Identity = 74/76 (97.37%), Postives = 76/76 (100.00%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.591 bits (366), Expect = 2.022e-34
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVK+KIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 67.0106 bits (162), Expect = 9.139e-11
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 3

Query:  525 MQIFVKTLTGKTITLEVESSDTIDNV*AKIQDKGGIP 635
            MQIFVKTLTGKTITLEVESSDTIDNV +KIQDK GIP
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIP 37

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBL1_ORYSJ (Ubiquitin-like protein 1 OS=Oryza sativa subsp. japonica GN=UBL1 PE=3 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 4.074e-35
Identity = 74/76 (97.37%), Postives = 75/76 (98.68%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTI NVKAK+QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIQNVKAKVQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 145.206 bits (365), Expect = 2.641e-34
Identity = 73/76 (96.05%), Postives = 74/76 (97.37%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTI NVKAK+QDKEGIPPDQQR IFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIQNVKAKVQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of FC868489 vs. ExPASy Swiss-Prot
Match: UBIQ_NEUCR (Ubiquitin OS=Neurospora crassa GN=ubi-1 PE=1 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 5.321e-35
Identity = 74/76 (97.37%), Postives = 75/76 (98.68%), Query Frame = 3

Query:   69 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 296
            MQIFVKTLTGKTITLEVESSDTIDNVK KIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 144.821 bits (364), Expect = 3.449e-34
Identity = 73/76 (96.05%), Postives = 74/76 (97.37%), Query Frame = 3

Query:  297 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRPIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 524
            MQIFVKTLTGKTITLEVESSDTIDNVK KIQDKEGIPPDQQR IFAGKQLEDGRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

The following BLAST results are available for this feature:

BLAST of FC868489 vs. ExPASy Swiss-Prot

BLAST of FC868489 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99


Match Name	E-value	Identity	Description
UBIQ_ARATH	6.285e-36	100.00	Ubiquitin OS=Arabidopsis thaliana GN=UBQ1 PE=1 SV=...	[more]
UBIQ_COPCO	1.400e-35	98.68	Ubiquitin OS=Coprinus congregatus PE=1 SV=1	[more]
UBIQ_CHLRE	1.829e-35	98.68	Ubiquitin OS=Chlamydomonas reinhardtii GN=UBI1 PE=...	[more]
UBIQ_YEAST	3.119e-35	97.37	Ubiquitin OS=Saccharomyces cerevisiae GN=UBI1 PE=1...	[more]
UBIQ_SCHPO	3.119e-35	97.37	Ubiquitin OS=Schizosaccharomyces pombe GN=ubi1 PE=...	[more]
UBIQ_KLULA	3.119e-35	97.37	Ubiquitin OS=Kluyveromyces lactis GN=ubi3 PE=1 SV=...	[more]
UBIQ_CRYNE	3.119e-35	97.37	Ubiquitin OS=Cryptococcus neoformans GN=UBI1 PE=1 ...	[more]
UBIQ_CANAL	3.119e-35	97.37	Ubiquitin OS=Candida albicans GN=UBI1 PE=1 SV=1	[more]
UBL1_ORYSJ	4.074e-35	97.37	Ubiquitin-like protein 1 OS=Oryza sativa subsp. ja...	[more]
UBIQ_NEUCR	5.321e-35	97.37	Ubiquitin OS=Neurospora crassa GN=ubi-1 PE=1 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	C31001A02EF AbioticL1 Citrus clementina cDNA clone C31001A02, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FC868489 ID=FC868489; Name=FC868489; organism=Citrus clementina; type=EST; length=636bp
GACCCAATCGGGTTATTCGATATTCTCAACAAATCTCTCCAATTCTCTCG
CTAAGAAACTTTTAAAAGATGCAGATCTTCGTGAAAACCCTAACCGGCAA
GACCATTACTCTCGAAGTCGAGAGCTCCGACACCATCGACAACGTCAAGG
CCAAGATCCAAGACAAGGAAGGAATCCCTCCGGACCAGCAGCGTCTGATC
TTCGCCGGAAAACAGCTCGAAGACGGCCGCACCTTGGCCGATTACAATAT
CCAAAAGGAATCGACGCTTCATCTGGTGCTTCGTCTCCGTGGAGGCATGC
AGATCTTCGTGAAAACCCTAACCGGCAAGACCATTACTCTCGAAGTTGAG
AGCTCCGACACGATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAAGG
GATTCCGCCGGACCAGCAGAGGCCGATCTTCGCCGGAAAGCAACTCGAGG
ACGGCCGCACCTTGGCCGATTACAATATCCAGAAGGAATCAACTCTTCAC
CTCGTGCTTCGTCTCCGTGGGGGCATGCAGATATTCGTCAAAACCCTAAC
GGGAAAGACGATTACCCTCGAAGTCGAGAGCTCCGACACGATCGACAACG
TCTAGGCCAAGATCCAGGACAAGGGAGGGATTCCGC