FC868749

Overview
NameFC868749
Unique NameFC868749
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length707
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBL1_SCHPO (Ubiquitin-like protein 1 OS=Schizosaccharomyces pombe GN=ubl1 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.701e-17
Identity = 40/76 (52.63%), Postives = 56/76 (73.68%), Query Frame = 1
Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          

HSP 2 Score: 88.1965 bits (217), Expect = 4.701e-17
Identity = 40/76 (52.63%), Postives = 56/76 (73.68%), Query Frame = 1
Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UCRP_MOUSE (Ubiquitin cross-reactive protein OS=Mus musculus GN=Isg15 PE=1 SV=4)

HSP 1 Score: 83.5741 bits (205), Expect = 1.158e-15
Identity = 51/149 (34.23%), Postives = 79/149 (53.02%), Query Frame = 1
Query:   82 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLR-LRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            VK L G    + V +S T+  +K +I  K G+P  QQRL      L+DG TL+   +   ST+ LV++     + I V+   G +   EV  + T+D +K K+  +E +  DQ  L F G+ +ED   L +Y ++ + T+   LRLRGG
Sbjct:    7 VKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGG 155          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: RUB1_YEAST (NEDD8-like protein RUB1 OS=Saccharomyces cerevisiae GN=RUB1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.158e-15
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 1
Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          

HSP 2 Score: 83.5741 bits (205), Expect = 1.158e-15
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 1
Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBD_HUMAN (Ubiquitin D OS=Homo sapiens GN=UBD PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 9.802e-15
Identity = 49/156 (31.41%), Postives = 81/156 (51.92%), Query Frame = 1
Query:   76 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFVKTL--TGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            + V++     +T +    D++  +K  ++ K  +P   Q L+   K L+  R+L+ Y I KE T+HL L++       + +F+       K   L+V  S ++  VKA I+ K GI P+ Q +   GK+LEDG+ +ADY I+K + L L     GG
Sbjct:   10 VHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGIRKGNLLFLACYCIGG 165          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UCRP_HUMAN (Interferon-induced 17 kDa protein OS=Homo sapiens GN=ISG15 PE=1 SV=5)

HSP 1 Score: 78.1814 bits (191), Expect = 4.865e-14
Identity = 52/151 (34.44%), Postives = 81/151 (53.64%), Query Frame = 1
Query:   82 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF--AGKQLEDGRTLADYNIQKESTLHLVL-RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            VK L G    + + SS ++  +KA+I  K G+   QQRL    +G  L+D   LA   +   ST+ LV+ +    + I V+   G++ T EV  + T+ ++K ++   EG+  D   L F GK LED   L +Y ++  ST+ + LRLRGG
Sbjct:    7 VKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG 157          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBD_RAT (Ubiquitin D OS=Rattus norvegicus GN=Ubd PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.565e-12
Identity = 44/146 (30.14%), Postives = 72/146 (49.32%), Query Frame = 1
Query:  106 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL------RGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            +T +   SD +  +   I+ +  +    Q L+   K L+  R L+ Y I KE+T+HL L++         + +      G+   L V  S ++  VK  I++   +PP +Q +   GK+LEDG+ +ADYNI+  S L L     GG
Sbjct:   16 MTFDTTMSDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRALSSYGIDKENTIHLTLKVVKPSDEELPLSLVESGDEGQRHLLRVRRSSSVAQVKEMIENVTAVPPKKQIVNCNGKRLEDGKIMADYNIKSGSLLFLTAHCIGG 161          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UCRP_BOVIN (Ubiquitin cross-reactive protein OS=Bos taurus GN=ISG15 PE=2 SV=3)

HSP 1 Score: 70.8626 bits (172), Expect = 7.767e-12
Identity = 47/150 (31.33%), Postives = 78/150 (52.00%), Query Frame = 1
Query:   82 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQ--LEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            VK L G+ I + +  S T+  +K  I  K  +P  QQRL     +  L++G  L    ++  ST+ LV++    + I V+   G++   EV+   T+  +K ++  KE +  DQ  L F G+ ++D   L +Y + K  T+ + LRLRGG
Sbjct:    7 VKMLGGQEILVPLRDSMTVSELKQFIAQKINVPAFQQRLAHLDSREVLQEGVPLVLQGLRAGSTVLLVVQ--NCISILVRNDKGRSSPYEVQLKQTVAELKQQVCQKERVQADQFWLSFEGRPMDDEHPLEEYGLMKGCTVFMNLRLRGG 154          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBD_MOUSE (Ubiquitin D OS=Mus musculus GN=Ubd PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.325e-11
Identity = 44/148 (29.73%), Postives = 73/148 (49.32%), Query Frame = 1
Query:  100 KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFV--KTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            + +T E   +D +  +   I+ +  +    Q L+   K L+  R L+ Y I KE+T+HL L++       + +F+      G+   L V  S ++  VK  I+    + P +Q +   GK+LEDG+ +ADYNI+  S L L     GG
Sbjct:   15 RLMTFETTENDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRKLSSYGIDKETTIHLTLKVVKPSDEELPLFLVESKNEGQRHLLRVRRSSSVAQVKEMIESVTSVIPKKQVVNCNGKKLEDGKIMADYNIKSGSLLFLTTHCTGG 162          
BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: ANUB1_HUMAN (AN1-type zinc finger and ubiquitin domain-containing protein 1 OS=Homo sapiens GN=ANUBL1 PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 3.855e-11
Identity = 53/161 (32.92%), Postives = 82/161 (50.93%), Query Frame = 1
Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF-AGKQLE--------DGRTLADYNIQKESTLHL-VLRLRG 522
            M++F++TLTG    L V   +T+ +VKAKI+  EGIP  +Q LI+   +LE+   L DYNI +  TL LVL +RGG    + T    T     + ++ +D+ + ++ +K         L++  G QL         DG      +  K+   HL VLR +G
Sbjct:   28 MELFIETLTGTCFELRVSPFETVISVKAKIRRLEGIPICRQHLIWNNMELENDYCLNDYNISEGCTLKLVLAMRGGP---INTRRVPTDDPLRKMAEYLDSSRVEVWEKTSCSKQVTFLVYQEGDQLNFFPAVDRGDGTLTPLSDSSKKIDFHLHVLRRKG 185          

HSP 2 Score: 68.1662 bits (165), Expect = 5.034e-11
Identity = 36/76 (47.37%), Postives = 50/76 (65.79%), Query Frame = 1
Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            M++F++TLTG    L V   +T+ +VKAKI+  EGIP  +Q LI+   +LE+   L DYNI +  TL LVL +RGG
Sbjct:   28 MELFIETLTGTCFELRVSPFETVISVKAKIRRLEGIPICRQHLIWNNMELENDYCLNDYNISEGCTLKLVLAMRGG 103          
The following BLAST results are available for this feature:
BLAST of FC868749 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
UBL1_SCHPO4.701e-1752.63Ubiquitin-like protein 1 OS=Schizosaccharomyces po... [more]
UCRP_MOUSE1.158e-1534.23Ubiquitin cross-reactive protein OS=Mus musculus G... [more]
RUB1_YEAST1.158e-1552.63NEDD8-like protein RUB1 OS=Saccharomyces cerevisia... [more]
UBD_HUMAN9.802e-1531.41Ubiquitin D OS=Homo sapiens GN=UBD PE=1 SV=2[more]
UCRP_HUMAN4.865e-1434.44Interferon-induced 17 kDa protein OS=Homo sapiens ... [more]
UBD_RAT1.565e-1230.14Ubiquitin D OS=Rattus norvegicus GN=Ubd PE=2 SV=1[more]
UCRP_BOVIN7.767e-1231.33Ubiquitin cross-reactive protein OS=Bos taurus GN=... [more]
UBD_MOUSE1.325e-1129.73Ubiquitin D OS=Mus musculus GN=Ubd PE=2 SV=1[more]
ANUB1_HUMAN3.855e-1132.92AN1-type zinc finger and ubiquitin domain-containi... [more]

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Properties
Property NameValue
Genbank descriptionC31004C11EF AbioticL1 Citrus clementina cDNA clone C31004C11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC868749 ID=FC868749; Name=FC868749; organism=Citrus clementina; type=EST; length=707bp
GAAAAATTCTCAGATTATCTTCTCCTTTAATTCGATAAAAGGCCTCCTTC
TGTTCTCTCTGTCAAGCAGATGCAAATCTTTGTGAAAACTCTCACCGGCA
AGACAATAACCCTTGAGGTTGAAAGCTCCGACACCATTGATAATGTAAAA
GCCAAGATCCAGGACAAGGAAGGGATCCCACCAGATCAGCAGAGGCTTAT
CTTTGCTGGTAAACAGCTCGAGGATGGTCGCACACTCGCTGACTATAATA
TCCAGAAGGAATCAACCCTCCACTTGGTCCTTCGACTTAGGGGTGGTATG
CAGATCTTTGTAAAGACCCTCACTGGCAAGACGATTACTCTTGAGGTGGA
AAGCTCCGACACCATTGACAATGTGAAAGCCAAGATTCAAGACAAGGAAG
GGATCCCACCAGACCAACAGAGGCTTATCTTTGCTGGTAAACAGCTTGAG
GATGGTCGCACACTTGCTGATTATAACATCCAGAAGGAGTCAACCCTCCA
CTTGGTCCTCCGTCTCCGTGGTGGTATGCAGATCTTTGTAAAGACCCTCA
CTGGAAAGACTATCACCCTTGAGGTGGAAAGCTCCGACACCATTGACAAT
GTCAAAGCCAAAATTCAGGATAAGGAAGGGATCCCACCAGACCAGNCAAG
GCTCATCTTTGCCGGTAAACACTAGAGGATGGACGCACACTTGCAGACTA
CACATCC
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