FC872958

Overview
NameFC872958
Unique NameFC872958
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length678
Libraries
Library NameType
AbsDevcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig1062 contig Ccv1_Contig1062:60..736. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX1_SCHPO (Glutathione peroxidase OS=Schizosaccharomyces pombe GN=gpx1 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 1.817e-30
Identity = 61/110 (55.45%), Postives = 73/110 (66.36%), Query Frame = 2 
Query:  290 YDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKSK 619
            YD   KD DG   P S  KGKV+L+VN AS+CG TP  Y  L  LY+KYK +GF IL FPCNQFG QEPGS+ EI +F    +   FP+  K++VNG N  PVYQ LKS+
Sbjct:    5 YDLAPKDKDGNPFPFSNLKGKVVLVVNTASKCGFTPQ-YKGLEALYQKYKDRGFIILGFPCNQFGNQEPGSDEEIAQFCQKNYGVTFPVLAKINVNGDNVDPVYQFLKSQ 113

HSP 2 Score: 24.2534 bits (51), Expect = 1.817e-30
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 3 
Query:  645 VKWNFEKFL 671
            +KWNFEKFL
Sbjct:  122 IKWNFEKFL 130
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX2_YEAST (Glutathione peroxidase 2 OS=Saccharomyces cerevisiae GN=GPX2 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.500e-29
Identity = 61/112 (54.46%), Postives = 73/112 (65.18%), Query Frame = 2 
Query:  284 SLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKSK 619
            S YD   KD  G+     + KGKV+LIVNVAS+CG TP  Y EL  LY+KY+ +GF IL FPCNQFG QEPGS+ +I EF    +   FPI  K+DVNG N   VY  LKS+
Sbjct:    4 SFYDLECKDKKGESFKFDQLKGKVVLIVNVASKCGFTPQ-YKELEELYKKYQDKGFVILGFPCNQFGKQEPGSDEQITEFCQLNYGVTFPIMKKIDVNGSNADSVYNYLKSQ 114

HSP 2 Score: 25.409 bits (54), Expect = 1.500e-29
Identity = 12/24 (50.00%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:  603 SY*SPSAGGILGDL-VKWNFEKFL 671
            +Y      G+LG   +KWNFEKFL
Sbjct:  109 NYLKSQKAGLLGFKGIKWNFEKFL 132
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPO_SYNY3 (Putative glutathione peroxidase OS=Synechocystis sp. (strain PCC 6803) GN=slr1171 PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 2.541e-29
Identity = 59/115 (51.30%), Postives = 77/115 (66.96%), Query Frame = 2 
Query:  266 TAATEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLL 610
            TA    ++Y F+   +DG  V L  F+GKVLLIVN AS+CG TP  Y  L  LY ++  +GF +L FPCNQFG QEPG + EIK F  TR+   FP+F+KV+VNGPN  P+++ L
Sbjct:    2 TAQANNTIYGFSANALDGSPVALRDFEGKVLLIVNTASQCGFTPQ-YQGLQALYNRFGDRGFTVLGFPCNQFGQQEPGGSGEIKNFCETRYGVTFPLFEKVEVNGPNAHPLFKFL 115

HSP 2 Score: 22.3274 bits (46), Expect = 2.541e-29
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P  GG   + +KWNF KFL
Sbjct:  125 PFLGG--AEDIKWNFTKFL 141
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX3_YEAST (Peroxiredoxin HYR1 OS=Saccharomyces cerevisiae GN=HYR1 PE=1 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 9.519e-29
Identity = 59/110 (53.64%), Postives = 73/110 (66.36%), Query Frame = 2 
Query:  290 YDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKSK 619
            Y     D  G+  P  + KGKV+LIVNVAS+CG TP  Y EL  LY++YK +GF I+ FPCNQFG QEPGS+ EI +F    +   FPI  K+DVNG N  PVY+ LKS+
Sbjct:    5 YKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFTPQ-YKELEALYKRYKDEGFTIIGFPCNQFGHQEPGSDEEIAQFCQLNYGVTFPIMKKIDVNGGNEDPVYKFLKSQ 113

HSP 2 Score: 24.2534 bits (51), Expect = 9.519e-29
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 3 
Query:  645 VKWNFEKFL 671
            +KWNFEKFL
Sbjct:  123 IKWNFEKFL 131
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPXA_NEIMF (Glutathione peroxidase homolog OS=Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) GN=gpxA PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.236e-28
Identity = 61/110 (55.45%), Postives = 76/110 (69.09%), Query Frame = 2 
Query:  287 LYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKS 616
            +YDF +KD +G  V LS ++GKVLLIVN A+RCGLTP  Y  L  LY +Y  +G EIL FPCNQF  Q P S+ EI +    +F  +F IFDK++VNG NTAP+Y  LKS
Sbjct:    3 IYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQ-YEALQKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANTAPLYAYLKS 111

HSP 2 Score: 21.9422 bits (45), Expect = 1.236e-28
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 3 
Query:  645 VKWNFEKFL 671
            +KWNF KFL
Sbjct:  141 IKWNFTKFL 149
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPXA_NEIMC (Glutathione peroxidase homolog OS=Neisseria meningitidis serogroup C GN=gpxA PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.236e-28
Identity = 61/110 (55.45%), Postives = 76/110 (69.09%), Query Frame = 2 
Query:  287 LYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKS 616
            +YDF +KD +G  V LS ++GKVLLIVN A+RCGLTP  Y  L  LY +Y  +G EIL FPCNQF  Q P S+ EI +    +F  +F IFDK++VNG NTAP+Y  LKS
Sbjct:    3 IYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQ-YEALQKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANTAPLYAYLKS 111

HSP 2 Score: 21.9422 bits (45), Expect = 1.236e-28
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 3 
Query:  645 VKWNFEKFL 671
            +KWNF KFL
Sbjct:  141 IKWNFTKFL 149
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPXA_NEIMB (Glutathione peroxidase homolog OS=Neisseria meningitidis serogroup B GN=gpxA PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.236e-28
Identity = 61/110 (55.45%), Postives = 76/110 (69.09%), Query Frame = 2 
Query:  287 LYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKS 616
            +YDF +KD +G  V LS ++GKVLLIVN A+RCGLTP  Y  L  LY +Y  +G EIL FPCNQF  Q P S+ EI +    +F  +F IFDK++VNG NTAP+Y  LKS
Sbjct:    3 IYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQ-YEALQKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANTAPLYAYLKS 111

HSP 2 Score: 21.9422 bits (45), Expect = 1.236e-28
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 3 
Query:  645 VKWNFEKFL 671
            +KWNF KFL
Sbjct:  141 IKWNFTKFL 149
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPXA_NEIMA (Glutathione peroxidase homolog OS=Neisseria meningitidis serogroup A GN=gpxA PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.236e-28
Identity = 61/110 (55.45%), Postives = 76/110 (69.09%), Query Frame = 2 
Query:  287 LYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKS 616
            +YDF +KD +G  V LS ++GKVLLIVN A+RCGLTP  Y  L  LY +Y  +G EIL FPCNQF  Q P S+ EI +    +F  +F IFDK++VNG NTAP+Y  LKS
Sbjct:    3 IYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQ-YEALQKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANTAPLYAYLKS 111

HSP 2 Score: 21.9422 bits (45), Expect = 1.236e-28
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 3 
Query:  645 VKWNFEKFL 671
            +KWNF KFL
Sbjct:  141 IKWNFTKFL 149
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_BOVIN (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Bos taurus GN=GPX4 PE=2 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 3.543e-28
Identity = 54/111 (48.65%), Postives = 78/111 (70.27%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG+ V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNAEIKEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 30.4166 bits (67), Expect = 3.543e-28
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   G+LG+ +KWNF KFL
Sbjct:  151 PKGRGMLGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX1_SCHMA (Glutathione peroxidase OS=Schistosoma mansoni GN=GPX1 PE=1 SV=2)

HSP 1 Score: 120.168 bits (300), Expect = 3.557e-28
Identity = 56/115 (48.70%), Postives = 78/115 (67.83%), Query Frame = 2 
Query:  284 SLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKSKCWG 628
            S+Y+FTVKDI+G DV L K++G V LIVNVA + G T  NY +L  ++ +   +G  ILAFPCNQFGGQEP +  EIK+F   ++  +F +F K+ VNG +   +Y+ LKS+  G
Sbjct:   10 SIYEFTVKDINGVDVSLEKYRGHVCLIVNVACKUGATDKNYRQLQEMHTRLVGKGLRILAFPCNQFGGQEPWAEAEIKKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQHG 124

HSP 2 Score: 24.2534 bits (51), Expect = 3.557e-28
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  627 GILGDLVKWNFEKFL 671
            G L + +KWNF KFL
Sbjct:  124 GTLTNNIKWNFSKFL 138
The following BLAST results are available for this feature:
BLAST of FC872958 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 110
Match NameE-valueIdentityDescription
GPX1_SCHPO1.817e-3055.45Glutathione peroxidase OS=Schizosaccharomyces pomb... [more]
GPX2_YEAST1.500e-2954.46Glutathione peroxidase 2 OS=Saccharomyces cerevisi... [more]
GPO_SYNY32.541e-2951.30Putative glutathione peroxidase OS=Synechocystis s... [more]
GPX3_YEAST9.519e-2953.64Peroxiredoxin HYR1 OS=Saccharomyces cerevisiae GN=... [more]
GPXA_NEIMF1.236e-2855.45Glutathione peroxidase homolog OS=Neisseria mening... [more]
GPXA_NEIMC1.236e-2855.45Glutathione peroxidase homolog OS=Neisseria mening... [more]
GPXA_NEIMB1.236e-2855.45Glutathione peroxidase homolog OS=Neisseria mening... [more]
GPXA_NEIMA1.236e-2855.45Glutathione peroxidase homolog OS=Neisseria mening... [more]
GPX4_BOVIN3.543e-2848.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX1_SCHMA3.557e-2848.70Glutathione peroxidase OS=Schistosoma mansoni GN=G... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI3DG11FM1 AbsDev Citrus clementina cDNA clone KN0AAI3DG11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC872958 ID=FC872958; Name=FC872958; organism=Citrus clementina; type=EST; length=678bp
ATTGCAACGCCCTTAAACCCCCGAACTGGTGTCGGACTCTATGTCGTTCG
AAGGATATCTGGCCTCACATAAACCGGATTTCACACCTAACAAAACATGC
CCTGCTGCATGGGCTACATCAATGGCGGCTTCATGGACTCCTACTAATTC
AATCAAAGCATCGATTGGGTCCGCTAAACCAGGCTTGCTTCAACATGGGC
TGTTTTCCCAATCTTCAAATTTGCCTGGGTTTTTCGTGAAACGTCGTTCC
TTTGGTGCTCACGCTACAGCTGCTACTGAGAAGTCTTTATATGACTTCAC
CGTCAAGGATATTGATGGTAAAGATGTTCCTCTTAGCAAGTTTAAGGGCA
AAGTTCTGTTGATTGTGAATGTCGCTTCAAGATGTGGCTTGACACCATCT
AATTACTCGGAGCTCTCTCACTTATATGAGAAGTACAAGACTCAAGGATT
TGAGATTCTAGCTTTCCCTTGCAATCAATTTGGTGGGCAAGAGCCTGGAT
CAAACCCTGAGATCAAGGAATTCGCTTGTACTAGGTTTAAGGCAGAATTT
CCCATATTTGACAAGGTTGATGTAAATGGACCAAACACAGCTCCAGTTTA
CCAGTTATTGAAGTCCAAGTGCTGGGGGAATTTTAGGCGACCTTGTCAAG
TGGAACTTTGAGAAGTTCTTAAGTGGAC
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