FC872958

Overview
NameFC872958
Unique NameFC872958
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length678
Libraries
Library NameType
AbsDevcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig1062 contig Ccv1_Contig1062:60..736. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPO_LACLA (Glutathione peroxidase OS=Lactococcus lactis subsp. lactis GN=gpo PE=3 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 3.566e-28
Identity = 56/115 (48.70%), Postives = 73/115 (63.48%), Query Frame = 2 
Query:  284 SLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKSKCWG 628
            + YDF+   ++G+ V +S FKGKV+++VN AS+CG TP  +  L  LYE YK QG EIL FPCNQF  Q+ G N EI EF    +   FP+F K+ VNG    P+YQ LK +  G
Sbjct:    2 NFYDFSAFKMNGETVSMSDFKGKVVIVVNTASKCGFTPQ-FEGLEKLYENYKDQGLEILGFPCNQFVNQDAGENSEINEFCQLNYGVTFPMFQKIKVNGKEAHPLYQFLKKEAKG 115

HSP 2 Score: 25.409 bits (54), Expect = 3.566e-28
Identity = 10/17 (58.82%), Postives = 11/17 (64.71%), Query Frame = 3 
Query:  621 AGGILGDLVKWNFEKFL 671
            A G L   +KWNF KFL
Sbjct:  113 AKGALSGTIKWNFTKFL 129
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_PONPY (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Pongo pygmaeus GN=GPX4 PE=2 SV=2)

HSP 1 Score: 111.694 bits (278), Expect = 7.818e-28
Identity = 54/111 (48.65%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 31.5722 bits (70), Expect = 7.818e-28
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   GILG+ +KWNF KFL
Sbjct:  151 PKGKGILGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_HUMAN (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Homo sapiens GN=GPX4 PE=1 SV=3)

HSP 1 Score: 111.694 bits (278), Expect = 7.818e-28
Identity = 54/111 (48.65%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 31.5722 bits (70), Expect = 7.818e-28
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   GILG+ +KWNF KFL
Sbjct:  151 PKGKGILGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_CEBAP (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Cebus apella GN=GPX4 PE=2 SV=2)

HSP 1 Score: 110.923 bits (276), Expect = 1.325e-27
Identity = 54/110 (49.09%), Postives = 76/110 (69.09%), Query Frame = 2 
Query:  284 SLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   40 SMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 31.5722 bits (70), Expect = 1.325e-27
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   GILG+ +KWNF KFL
Sbjct:  151 PKGKGILGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_CALJA (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Callithrix jacchus GN=GPX4 PE=2 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 1.725e-27
Identity = 54/111 (48.65%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGHTVNLDKYRGFVCIVTNVASQUGKTQVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 29.6462 bits (65), Expect = 1.725e-27
Identity = 11/19 (57.89%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   G LG+ +KWNF KFL
Sbjct:  151 PKGKGTLGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_MACFU (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Macaca fuscata fuscata GN=GPX4 PE=2 SV=2)

HSP 1 Score: 109.768 bits (273), Expect = 2.923e-27
Identity = 53/111 (47.75%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN +IKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGVRILAFPCNQFGKQEPGSNEKIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 31.5722 bits (70), Expect = 2.923e-27
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   GILG+ +KWNF KFL
Sbjct:  151 PKGKGILGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPO_LACLM (Glutathione peroxidase OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=gpo PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.943e-27
Identity = 54/115 (46.96%), Postives = 72/115 (62.61%), Query Frame = 2 
Query:  284 SLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLKSKCWG 628
            + YDF+   ++G+ V +S +KGKV+++VN AS+CG TP  +  L  LYE YK QG EIL FPCNQF  Q+ G N EI EF    +   F +F K+ VNG    P+YQ LK +  G
Sbjct:    2 NFYDFSAVKMNGETVSMSDYKGKVVIVVNTASKCGFTPQ-FEGLEKLYETYKDQGLEILGFPCNQFANQDAGENTEINEFCQLNYGVTFTMFQKIKVNGKEAHPLYQFLKKEAKG 115

HSP 2 Score: 25.409 bits (54), Expect = 2.943e-27
Identity = 10/17 (58.82%), Postives = 11/17 (64.71%), Query Frame = 3 
Query:  621 AGGILGDLVKWNFEKFL 671
            A G L   +KWNF KFL
Sbjct:  113 AKGALSGTIKWNFTKFL 129
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX42_MOUSE (Phospholipid hydroperoxide glutathione peroxidase, nuclear OS=Mus musculus GN=Gpx4 PE=2 SV=3)

HSP 1 Score: 110.153 bits (274), Expect = 4.925e-27
Identity = 53/111 (47.75%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F ++ K+ VNG +  P+++ +K
Sbjct:   95 RSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVKFDMYSKICVNGDDAHPLWKWMK 204

HSP 2 Score: 30.4166 bits (67), Expect = 4.925e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   G+LG+ +KWNF KFL
Sbjct:  207 PKGRGMLGNAIKWNFTKFL 225
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX4_PIG (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Sus scrofa GN=GPX4 PE=1 SV=3)

HSP 1 Score: 110.153 bits (274), Expect = 4.954e-27
Identity = 53/111 (47.75%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGS+ EIKEFA   +  +F +F K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSDAEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 30.4166 bits (67), Expect = 4.954e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   G+LG+ +KWNF KFL
Sbjct:  151 PKGRGMLGNAIKWNFTKFL 169
[Close]
BLAST of FC872958 vs. ExPASy Swiss-Prot
Match: GPX41_MOUSE (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Mus musculus GN=Gpx4 PE=1 SV=4)

HSP 1 Score: 110.153 bits (274), Expect = 4.954e-27
Identity = 53/111 (47.75%), Postives = 77/111 (69.37%), Query Frame = 2 
Query:  281 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQLLK 613
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F ++ K+ VNG +  P+++ +K
Sbjct:   39 RSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVKFDMYSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 30.4166 bits (67), Expect = 4.954e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:  615 PSAGGILGDLVKWNFEKFL 671
            P   G+LG+ +KWNF KFL
Sbjct:  151 PKGRGMLGNAIKWNFTKFL 169
The following BLAST results are available for this feature:
BLAST of FC872958 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 110
Match NameE-valueIdentityDescription
GPO_LACLA3.566e-2848.70Glutathione peroxidase OS=Lactococcus lactis subsp... [more]
GPX4_PONPY7.818e-2848.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_HUMAN7.818e-2848.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_CEBAP1.325e-2749.09Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_CALJA1.725e-2748.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_MACFU2.923e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPO_LACLM2.943e-2746.96Glutathione peroxidase OS=Lactococcus lactis subsp... [more]
GPX42_MOUSE4.925e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_PIG4.954e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX41_MOUSE4.954e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI3DG11FM1 AbsDev Citrus clementina cDNA clone KN0AAI3DG11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC872958 ID=FC872958; Name=FC872958; organism=Citrus clementina; type=EST; length=678bp
ATTGCAACGCCCTTAAACCCCCGAACTGGTGTCGGACTCTATGTCGTTCG
AAGGATATCTGGCCTCACATAAACCGGATTTCACACCTAACAAAACATGC
CCTGCTGCATGGGCTACATCAATGGCGGCTTCATGGACTCCTACTAATTC
AATCAAAGCATCGATTGGGTCCGCTAAACCAGGCTTGCTTCAACATGGGC
TGTTTTCCCAATCTTCAAATTTGCCTGGGTTTTTCGTGAAACGTCGTTCC
TTTGGTGCTCACGCTACAGCTGCTACTGAGAAGTCTTTATATGACTTCAC
CGTCAAGGATATTGATGGTAAAGATGTTCCTCTTAGCAAGTTTAAGGGCA
AAGTTCTGTTGATTGTGAATGTCGCTTCAAGATGTGGCTTGACACCATCT
AATTACTCGGAGCTCTCTCACTTATATGAGAAGTACAAGACTCAAGGATT
TGAGATTCTAGCTTTCCCTTGCAATCAATTTGGTGGGCAAGAGCCTGGAT
CAAACCCTGAGATCAAGGAATTCGCTTGTACTAGGTTTAAGGCAGAATTT
CCCATATTTGACAAGGTTGATGTAAATGGACCAAACACAGCTCCAGTTTA
CCAGTTATTGAAGTCCAAGTGCTGGGGGAATTTTAGGCGACCTTGTCAAG
TGGAACTTTGAGAAGTTCTTAAGTGGAC
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