FC920387

Overview
NameFC920387
Unique NameFC920387
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length672
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig789 contig Ccv1_Contig789:131..803. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_DAUCA (Elongation factor 1-alpha OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 367.081 bits (941), Expect = 4.727e-101
Identity = 179/187 (95.72%), Postives = 183/187 (97.86%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTPRK 643
            MGKEK HI+IVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGI IDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK+R++EIVKEVSSYLKKVGY P K
Sbjct:    1 MGKEKIHISIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKSRFEEIVKEVSSYLKKVGYNPDK 187
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_HORVU (Elongation factor 1-alpha OS=Hordeum vulgare GN=BLT63 PE=1 SV=1)

HSP 1 Score: 362.459 bits (929), Expect = 1.164e-99
Identity = 178/187 (95.19%), Postives = 180/187 (96.26%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTPRK 643
            MGKEK HI+IVVIGHVDSGKSTTTGHLIYKLGGIDKRVIE FEKEAAEMNKRSFKYAWVLDKLKAERERGI IDIALWKFETTKY CTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVK MICCCNKMDATTPKYSK+RYDEIVKEVSSYLKKVGY P K
Sbjct:    1 MGKEKIHISIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIESFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYSCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKHMICCCNKMDATTPKYSKSRYDEIVKEVSSYLKKVGYNPDK 187
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A1_HORVU (Elongation factor 1-alpha OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 362.073 bits (928), Expect = 1.521e-99
Identity = 178/187 (95.19%), Postives = 181/187 (96.79%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTPRK 643
            MGKEK HI+IVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGI IDIALWKFETTKY CTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTG F+AGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK+RYDEIVKEVSSYLKKVGY P K
Sbjct:    1 MGKEKIHISIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYSCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGVFQAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKSRYDEIVKEVSSYLKKVGYNPDK 187
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A_PEA (Elongation factor 1-alpha OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 350.903 bits (899), Expect = 3.506e-96
Identity = 173/187 (92.51%), Postives = 178/187 (95.19%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTPRK 643
            MGKEK HINIVVIGHVDSGKSTTT H+IYKLGGIDKRVIERFEKEA EMNKRSFKYAW+LDKLKAERERGI IDIAL KFETTKYY TV+DAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK RY+EIVKEVSSYLK+VGY P K
Sbjct:    1 MGKEKVHINIVVIGHVDSGKSTTTVHVIYKLGGIDKRVIERFEKEADEMNKRSFKYAWLLDKLKAERERGITIDIALLKFETTKYYSTVMDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKGRYEEIVKEVSSYLKEVGYNPDK 187
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_RAT (Elongation factor 1-alpha 2 OS=Rattus norvegicus GN=Eef1a2 PE=2 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 6.719e-87
Identity = 155/185 (83.78%), Postives = 168/185 (90.81%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTP 637
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGI IDI+LWKFETTKYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RYDEIVKEVS+Y+KK+GY P
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNP 185

HSP 2 Score: 23.483 bits (49), Expect = 6.719e-87
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  631 HPEKVPFVPISGF 669
            +P  VPFVPISG+
Sbjct:  184 NPATVPFVPISGW 196
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_RABIT (Elongation factor 1-alpha 2 OS=Oryctolagus cuniculus GN=EEF1A2 PE=1 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 6.719e-87
Identity = 155/185 (83.78%), Postives = 168/185 (90.81%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTP 637
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGI IDI+LWKFETTKYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RYDEIVKEVS+Y+KK+GY P
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNP 185

HSP 2 Score: 23.483 bits (49), Expect = 6.719e-87
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  631 HPEKVPFVPISGF 669
            +P  VPFVPISG+
Sbjct:  184 NPATVPFVPISGW 196
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_MOUSE (Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef1a2 PE=1 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 6.719e-87
Identity = 155/185 (83.78%), Postives = 168/185 (90.81%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTP 637
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGI IDI+LWKFETTKYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RYDEIVKEVS+Y+KK+GY P
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNP 185

HSP 2 Score: 23.483 bits (49), Expect = 6.719e-87
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  631 HPEKVPFVPISGF 669
            +P  VPFVPISG+
Sbjct:  184 NPATVPFVPISGW 196
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_HUMAN (Elongation factor 1-alpha 2 OS=Homo sapiens GN=EEF1A2 PE=1 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 6.719e-87
Identity = 155/185 (83.78%), Postives = 168/185 (90.81%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTP 637
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGI IDI+LWKFETTKYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RYDEIVKEVS+Y+KK+GY P
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNP 185

HSP 2 Score: 23.483 bits (49), Expect = 6.719e-87
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  631 HPEKVPFVPISGF 669
            +P  VPFVPISG+
Sbjct:  184 NPATVPFVPISGW 196
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A2_BOVIN (Elongation factor 1-alpha 2 OS=Bos taurus GN=EEF1A2 PE=2 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 6.719e-87
Identity = 155/185 (83.78%), Postives = 168/185 (90.81%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTP 637
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGI IDI+LWKFETTKYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RYDEIVKEVS+Y+KK+GY P
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNP 185

HSP 2 Score: 23.483 bits (49), Expect = 6.719e-87
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 1 
Query:  631 HPEKVPFVPISGF 669
            +P  VPFVPISG+
Sbjct:  184 NPATVPFVPISGW 196
[Close]
BLAST of FC920387 vs. ExPASy Swiss-Prot
Match: EF1A_DICDI (Elongation factor 1-alpha OS=Dictyostelium discoideum GN=eef1a1 PE=1 SV=2)

HSP 1 Score: 319.701 bits (818), Expect = 8.656e-87
Identity = 154/187 (82.35%), Postives = 171/187 (91.44%), Query Frame = 2 
Query:   83 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGIAIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYTPRK 643
            MGKEKTHINIVVIGHVD+GKSTTTGHLIYK GGIDKRVIE++EKEA+EM K+SFKYAWV+DKLKAERERGI IDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVL+I S TG FEAGI+K+GQTREHALLA+TLGVKQMI   NKMD  +  YS+ARYDEIVKEVSS++KK+GY P K
Sbjct:    1 MGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEIVKEVSSFIKKIGYNPEK 187
The following BLAST results are available for this feature:
BLAST of FC920387 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A2_DAUCA4.727e-10195.72Elongation factor 1-alpha OS=Daucus carota PE=2 SV... [more]
EF1A2_HORVU1.164e-9995.19Elongation factor 1-alpha OS=Hordeum vulgare GN=BL... [more]
EF1A1_HORVU1.521e-9995.19Elongation factor 1-alpha OS=Hordeum vulgare PE=1 ... [more]
EF1A_PEA3.506e-9692.51Elongation factor 1-alpha OS=Pisum sativum PE=2 SV... [more]
EF1A2_RAT6.719e-8783.78Elongation factor 1-alpha 2 OS=Rattus norvegicus G... [more]
EF1A2_RABIT6.719e-8783.78Elongation factor 1-alpha 2 OS=Oryctolagus cunicul... [more]
EF1A2_MOUSE6.719e-8783.78Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef... [more]
EF1A2_HUMAN6.719e-8783.78Elongation factor 1-alpha 2 OS=Homo sapiens GN=EEF... [more]
EF1A2_BOVIN6.719e-8783.78Elongation factor 1-alpha 2 OS=Bos taurus GN=EEF1A... [more]
EF1A_DICDI8.656e-8782.35Elongation factor 1-alpha OS=Dictyostelium discoid... [more]

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Properties
Property NameValue
Genbank descriptionC32007F09EF RVDevelop1 Citrus clementina cDNA clone C32007F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920387 ID=FC920387; Name=FC920387; organism=Citrus clementina; type=EST; length=672bp
ACTCTCTCAATTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATAT
CATTTTAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACT
CACATCAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCAC
CACCGGACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCG
AGAGGTTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATAC
GCCTGGGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTGCCAT
TGATATTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCA
TTGATGCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACC
TCACAGGCTGACTGTGCCGTCCTCATCATTGACTCCACCACCGGTGGTTT
TGAAGCTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTG
CTTTCACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGAC
GCCACCACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGA
GGTCTCTTCCTACCTGAAGAAGGTTGGATACACCCCGAGAAAGTCCCATT
CGTGCCCATCTCTGGTTTCGAG
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