BQ622995

Overview
NameBQ622995
Unique NameBQ622995
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length660
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A3_OSCTI (Elongation factor 1-alpha 3 OS=Oscheius tipulae GN=eft-3 PE=3 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.258e-50
Identity = 94/133 (70.68%), Postives = 114/133 (85.71%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKD++RG V S+SK+DPA+E+ NFT+QVI+MNHPGQI  GY PVLDCHT+HIA KF+EL  K+DRR+GK  E  PKFLK+GDAG+V++IPTKP  VE+F+DY PLGRFAVRDMRQTVAVGVIKSV K D +G
Sbjct:  317 SVKDIRRGSVCSDSKNDPAKESKNFTAQVIVMNHPGQISAGYTPVLDCHTAHIACKFAELKEKVDRRTGKSTEASPKFLKSGDAGIVELIPTKPPCVESFTDYAPLGRFAVRDMRQTVAVGVIKSVTKDDGSG 449          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A1_OSCTI (Elongation factor 1-alpha 1 OS=Oscheius tipulae GN=eft-1 PE=3 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.258e-50
Identity = 93/133 (69.92%), Postives = 116/133 (87.22%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKD++RG V S+SK+DPA+E+ +FT+QVI+MNHPGQIG GY PVLDCHT+HIA KF+EL  K+DRR+GK++E  PKFLK+GDAG+V++IPTKP+ VE F+DY PLGRFAVRDMRQTVAVGVIK V K D +G
Sbjct:  316 SVKDIRRGSVCSDSKNDPAKESKSFTAQVIVMNHPGQIGAGYTPVLDCHTAHIACKFAELKEKVDRRTGKKVEDLPKFLKSGDAGIVELIPTKPLCVEAFTDYAPLGRFAVRDMRQTVAVGVIKGVTKDDGSG 448          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A3_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-3 PE=3 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.643e-50
Identity = 95/133 (71.43%), Postives = 117/133 (87.97%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKD++RG V S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA KFSEL+ KIDRRS K +E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGVIK+VEK D  G
Sbjct:  314 SVKDIRRGNVCSDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFSELIEKIDRRSEK-MEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGVIKAVEKVDKAG 445          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_ONCVO (Elongation factor 1-alpha OS=Onchocerca volvulus PE=2 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 2.146e-50
Identity = 92/133 (69.17%), Postives = 115/133 (86.47%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            ++KD++RG VAS+SK+DPA+E   FT+QVIIMNHPGQI  GY PVLDCHT+HIA KF+EL  K+DRRSGK++E  PK LK+GDAG++ +IPTKP+ VETF++YPPLGRFAVRDMRQTVAVGVIK+V+K +  G
Sbjct:  316 SIKDIRRGSVASDSKNDPAKETKMFTAQVIIMNHPGQISAGYTPVLDCHTAHIACKFAELKEKVDRRSGKKVEDNPKSLKSGDAGIIDLIPTKPLCVETFTEYPPLGRFAVRDMRQTVAVGVIKNVDKSEGVG 448          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1AC_PORPU (Elongation factor 1-alpha C OS=Porphyra purpurea GN=TEF-C PE=2 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 2.146e-50
Identity = 94/133 (70.68%), Postives = 113/133 (84.96%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKDLKRG+V  +SK+DP +  A+F +QVII+NHPG+I  GYAPVLDCHT+HIA KFSEL+ K+DRRSGK+LE  PK +K+GDA MVKM+ +KPM VE F+ YPPLGRFAVRDMRQTVAVGVIKSVEKK+  G
Sbjct:  308 SVKDLKRGYVCGDSKNDPPKGCASFNAQVIILNHPGEIHAGYAPVLDCHTAHIACKFSELILKMDRRSGKKLEDSPKMIKSGDAAMVKMVASKPMCVEAFTSYPPLGRFAVRDMRQTVAVGVIKSVEKKEVEG 440          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_PORYE (Elongation factor 1-alpha OS=Porphyra yezoensis PE=2 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 2.802e-50
Identity = 94/133 (70.68%), Postives = 113/133 (84.96%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKDLKRG+V  +SK+DP +  A+F +QVII+NHPG+I  GYAPVLDCHT+HIA KFSEL+ K+DRRSGK+LE  PK +K+GDA MVKM+ +KPM VE F+ YPPLGRFAVRDMRQTVAVGVIKSVEKK+  G
Sbjct:  308 SVKDLKRGYVCGDSKNDPPKGCASFNAQVIILNHPGEIHAGYAPVLDCHTAHIACKFSELILKMDRRSGKKLEDTPKMIKSGDAAMVKMVASKPMCVEAFTQYPPLGRFAVRDMRQTVAVGVIKSVEKKEVEG 440          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_NEUCR (Elongation factor 1-alpha OS=Neurospora crassa GN=tef-1 PE=3 SV=2)

HSP 1 Score: 198.364 bits (503), Expect = 2.802e-50
Identity = 92/128 (71.88%), Postives = 114/128 (89.06%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 386
            +VKD++RG VA +SK+DP   AA+FT+QVI++NHPGQ+G GYAPVLDCHT+HIA KF+EL+ KIDRR+GK +E  PKF+K+GDA +VKMIP+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+V+K
Sbjct:  315 SVKDIRRGNVAGDSKNDPPAGAASFTAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFAELLEKIDRRTGKAVEASPKFIKSGDAAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVDK 442          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_YARLI (Elongation factor 1-alpha OS=Yarrowia lipolytica GN=TEF PE=2 SV=2)

HSP 1 Score: 197.978 bits (502), Expect = 3.660e-50
Identity = 91/133 (68.42%), Postives = 113/133 (84.96%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKD++RG VA +SK+DP +   +F +QVI++NHPGQIG GYAPVLDCHT+HIA KF  L+ KIDRR+GK++E  PKF+K+GDA +VKM+P+KPM VE F++YPPLGRFAVRDMRQTVAVGVIKSVEK D  G
Sbjct:  315 SVKDIRRGNVAGDSKNDPPKGCDSFNAQVIVLNHPGQIGAGYAPVLDCHTAHIACKFDTLIEKIDRRTGKKMEDSPKFIKSGDAAIVKMVPSKPMCVEAFTEYPPLGRFAVRDMRQTVAVGVIKSVEKSDKAG 447          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_AURPU (Elongation factor 1-alpha OS=Aureobasidium pullulans GN=TEF1 PE=3 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 3.660e-50
Identity = 92/133 (69.17%), Postives = 113/133 (84.96%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VK+++RG VA +SK+DP +   +F +QVI++NHPGQ+G GYAPVLDCHT+HIA KFSELV KIDRR+GK +E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIKSV K D  G
Sbjct:  313 SVKEIRRGNVAGDSKNDPPKGCDSFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACKFSELVEKIDRRTGKSVEAAPKFIKSGDAAIVKMVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVAKSDKQG 445          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A3_HUMAN (Putative elongation factor 1-alpha-like 3 OS=Homo sapiens GN=EEF1AL3 PE=5 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 3.660e-50
Identity = 96/129 (74.42%), Postives = 112/129 (86.82%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKK 389
            +VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCH +HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGVIK+V+KK
Sbjct:  316 SVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHMAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKK 444          
The following BLAST results are available for this feature:
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A3_OSCTI1.258e-5070.68Elongation factor 1-alpha 3 OS=Oscheius tipulae GN... [more]
EF1A1_OSCTI1.258e-5069.92Elongation factor 1-alpha 1 OS=Oscheius tipulae GN... [more]
EF1A3_RHIRA1.643e-5071.43Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A_ONCVO2.146e-5069.17Elongation factor 1-alpha OS=Onchocerca volvulus P... [more]
EF1AC_PORPU2.146e-5070.68Elongation factor 1-alpha C OS=Porphyra purpurea G... [more]
EF1A_PORYE2.802e-5070.68Elongation factor 1-alpha OS=Porphyra yezoensis PE... [more]
EF1A_NEUCR2.802e-5071.88Elongation factor 1-alpha OS=Neurospora crassa GN=... [more]
EF1A_YARLI3.660e-5068.42Elongation factor 1-alpha OS=Yarrowia lipolytica G... [more]
EF1A_AURPU3.660e-5069.17Elongation factor 1-alpha OS=Aureobasidium pullula... [more]
EF1A3_HUMAN3.660e-5074.42Putative elongation factor 1-alpha-like 3 OS=Homo ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00086 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00086 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ622995 ID=BQ622995; Name=BQ622995; organism=Citrus sinensis; type=EST; length=660bp
TTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTCCAACTCGAAGGAT
GATCCGGCCAGGGAAGCTGCCAACTTCACCTCACAGGTCATCATCATGAA
CCACCCTGGCCAGATTGGAAATGGATATGCCCCAGTGCTTGACTGCCACA
CCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGACGAAGATTGATAGG
CGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCTTGAAGAATGGAGA
CGCCGGAATGGTTAAGATGATTCCCACCAAGCCCATGGTTGTTGAAACCT
TCTCCGATTACCCACCCCTTGGTCGTTTTGCTGTTAGGGACATGCGTCAG
ACTGTTGCTGTGGGTGTTATCAAGAGTGTTGAGAAGAAGGATCCCACAGG
TGCGAAGGTCACCAAGTCCGCAGCCAAGAAGAAATGAGAAATTTTTATGG
GTCTGTCTACAATAGTTAGGGTTTATTTTCTGTCTTTTTTTATTTTCTTG
TCTGTTTTTGTTTTGCATCAATGTTTCTGCAAACAGCCCTATTTGCTTCC
ACCAGTTTGCTGTTGAGCAATTGGGATGTTATCTTTCTGCTTAACTGGCT
GTGGGTGGGTGTTCGGATTTTTATATTCTGAATTTGGTTAATAGCACTAA
ATTGAGTTGT
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