BQ622995

Overview
NameBQ622995
Unique NameBQ622995
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length660
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A1_SCHPO (Elongation factor 1-alpha-A OS=Schizosaccharomyces pombe GN=tef1a PE=1 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 2.008e-48
Identity = 90/131 (68.70%), Postives = 112/131 (85.50%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDP 395
            +VKD++RG V  +SK+DP    A+FT+QVII+NHPGQI  GY+PVLDCHT+HIA KF+EL+ KIDRRSGK++E+ PKF+K+GDA + KM+P+KPM VE F+DY PLGRFAVRDMRQTVAVGVIK+VEK  P
Sbjct:  314 SVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_TETPY (Elongation factor 1-alpha OS=Tetrahymena pyriformis PE=2 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 2.623e-48
Identity = 91/130 (70.00%), Postives = 109/130 (83.85%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKD 392
            +VKD++RG VAS++K+DPA+EAA F SQVIIMNHPGQI  GY PVLDCHT+HIA KF  +  KIDRR+GK  E+ PKF+KNGDA +V +IPTK + VE F +YPPLGR+AVRDM+QTVAVGVIK VEKKD
Sbjct:  305 SVKDIRRGNVASDAKNDPAKEAATFYSQVIIMNHPGQIQAGYTPVLDCHTAHIACKFETIHDKIDRRTGKSQEENPKFIKNGDAALVTLIPTKALCVEVFQEYPPLGRYAVRDMKQTVAVGVIKKVEKKD 434          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_EIMBO (Elongation factor 1-alpha (Fragment) OS=Eimeria bovis PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 3.426e-48
Identity = 89/133 (66.92%), Postives = 114/133 (85.71%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VKD+KRG VAS+SK+DPA+ AA+F +QVI+++HPGQI  GY+PV+DCHT+HIA KF+ L  ++DRRSGK LE +PKF+K GDA ++KM P+KPM VE+F +YPPLGRFAVRDM+QTVAVGVIK VEKK+  G
Sbjct:  201 SVKDVKRGHVASDSKNDPAKAAASFQAQVIVLHHPGQINPGYSPVVDCHTAHIACKFAVLEKRLDRRSGKALEDDPKFIKTGDAAIIKMEPSKPMCVESFIEYPPLGRFAVRDMKQTVAVGVIKGVEKKEAGG 333          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A1_DROME (Elongation factor 1-alpha 1 OS=Drosophila melanogaster GN=Ef1alpha48D PE=1 SV=2)

HSP 1 Score: 191.045 bits (484), Expect = 4.474e-48
Identity = 86/133 (64.66%), Postives = 113/133 (84.96%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VK+L+RG+VA +SK +P + AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRRSGK  E+ PKF+K+GDA +V ++P+KP+ VE F ++PPLGRFAVRDMRQTVAVGVIK+V  KD +G
Sbjct:  316 SVKELRRGYVAGDSKANPPKGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKVDRRSGKTTEENPKFIKSGDAAIVNLVPSKPLCVEAFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKDASG 448          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_HYDAT (Elongation factor 1-alpha OS=Hydra attenuata PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.700e-47
Identity = 89/128 (69.53%), Postives = 111/128 (86.72%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEK 386
            ++KD++RG VAS+SK+DPA EAA+F +QVII+NHPG+I  GY PVLDCHT+HIA KF+EL+ KIDRRSGK +E EPK +K+GDA ++ +IP+K M VE+FS YPPLGRFAVRDMRQTVAVGVIK V+K
Sbjct:  318 SIKDIRRGMVASDSKNDPAIEAASFKAQVIILNHPGEIHAGYQPVLDCHTAHIACKFAELLEKIDRRSGKVIETEPKMVKSGDAAIINLIPSKGMCVESFSQYPPLGRFAVRDMRQTVAVGVIKEVDK 445          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_CRYPV (Elongation factor 1-alpha OS=Cryptosporidium parvum PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.700e-47
Identity = 85/132 (64.39%), Postives = 112/132 (84.85%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPT 398
            ++KD+KRGFVAS++K+DPA+   +FT+QVI++NHPG+I NGY+PV+DCHT+HI+ KF  +  K+D+RSGK LE+ PK +K+GDA +V M P KP+ VE F+DYPPLGRFAVRDM+QTVAVGVIKSV KK+ T
Sbjct:  300 SIKDIKRGFVASDAKNDPAKGCEDFTAQVIVLNHPGEIKNGYSPVVDCHTAHISCKFQTITAKMDKRSGKVLEENPKLIKSGDAALVVMQPLKPLCVEAFTDYPPLGRFAVRDMKQTVAVGVIKSVTKKEAT 431          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_ASHGO (Elongation factor 1-alpha OS=Ashbya gossypii GN=TEF PE=3 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.700e-47
Identity = 89/133 (66.92%), Postives = 108/133 (81.20%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VK+++RG V  +SK+DP + A +F + VI++NHPGQI  GY+PVLDCHT+HIA KF EL+ K DRR+GK+LE  PKFLK GDA MVK +P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIKSV K D  G
Sbjct:  314 SVKEIRRGNVCGDSKNDPPKAAESFNATVIVLNHPGQISAGYSPVLDCHTAHIACKFDELLEKNDRRTGKKLEDSPKFLKAGDAAMVKFVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVVKSDKAG 446          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A2_DROME (Elongation factor 1-alpha 2 OS=Drosophila melanogaster GN=Ef1alpha100E PE=1 SV=2)

HSP 1 Score: 187.963 bits (476), Expect = 3.788e-47
Identity = 86/132 (65.15%), Postives = 112/132 (84.85%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPT 398
            +VK+L+RG+VA +SK++P R AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KFSE+  K DRR+GK  E EPK +K+GDA ++ ++P+KP+ VE+F ++PPLGRFAVRDMRQTVAVGVIKSV  K+ T
Sbjct:  316 SVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFSEIKEKCDRRTGKTTETEPKAIKSGDAAIIVLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKSVNFKETT 447          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_APIME (Elongation factor 1-alpha OS=Apis mellifera PE=3 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 6.461e-47
Identity = 88/133 (66.17%), Postives = 109/133 (81.95%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VK+L+RG+VA +SK+ P R AA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K DRR+GK  E+ PK +K+GDA +V + PTKPM VE F ++PPLGRFAVRDMRQTVAVGVIKSV  KD  G
Sbjct:  316 SVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQG 448          
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Match: EF1A_RHYAM (Elongation factor 1-alpha (Fragment) OS=Rhynchosciara americana PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.102e-46
Identity = 87/133 (65.41%), Postives = 110/133 (82.71%), Query Frame = 3
Query:    3 AVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKSVEKKDPTG 401
            +VK+L+RG+VA +SK +P + A NFT+QVI++NHPGQI NGY PVLDCHT+HIA KF+E+  K+DRRSGK  E  PK +K+GDA +V + P+KP+ VE+F ++PPLGRFAVRDMRQTVAVGVIKSV  KD TG
Sbjct:  265 SVKELRRGYVAGDSKANPPKGAENFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKXKVDRRSGKSTEDNPKAIKSGDAAIVILQPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKSVVFKDATG 397          
The following BLAST results are available for this feature:
BLAST of BQ622995 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 171
Match NameE-valueIdentityDescription
EF1A1_SCHPO2.008e-4868.70Elongation factor 1-alpha-A OS=Schizosaccharomyces... [more]
EF1A_TETPY2.623e-4870.00Elongation factor 1-alpha OS=Tetrahymena pyriformi... [more]
EF1A_EIMBO3.426e-4866.92Elongation factor 1-alpha (Fragment) OS=Eimeria bo... [more]
EF1A1_DROME4.474e-4864.66Elongation factor 1-alpha 1 OS=Drosophila melanoga... [more]
EF1A_HYDAT1.700e-4769.53Elongation factor 1-alpha OS=Hydra attenuata PE=2 ... [more]
EF1A_CRYPV1.700e-4764.39Elongation factor 1-alpha OS=Cryptosporidium parvu... [more]
EF1A_ASHGO1.700e-4766.92Elongation factor 1-alpha OS=Ashbya gossypii GN=TE... [more]
EF1A2_DROME3.788e-4765.15Elongation factor 1-alpha 2 OS=Drosophila melanoga... [more]
EF1A_APIME6.461e-4766.17Elongation factor 1-alpha OS=Apis mellifera PE=3 S... [more]
EF1A_RHYAM1.102e-4665.41Elongation factor 1-alpha (Fragment) OS=Rhynchosci... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00086 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00086 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ622995 ID=BQ622995; Name=BQ622995; organism=Citrus sinensis; type=EST; length=660bp
TTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTCCAACTCGAAGGAT
GATCCGGCCAGGGAAGCTGCCAACTTCACCTCACAGGTCATCATCATGAA
CCACCCTGGCCAGATTGGAAATGGATATGCCCCAGTGCTTGACTGCCACA
CCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGACGAAGATTGATAGG
CGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCTTGAAGAATGGAGA
CGCCGGAATGGTTAAGATGATTCCCACCAAGCCCATGGTTGTTGAAACCT
TCTCCGATTACCCACCCCTTGGTCGTTTTGCTGTTAGGGACATGCGTCAG
ACTGTTGCTGTGGGTGTTATCAAGAGTGTTGAGAAGAAGGATCCCACAGG
TGCGAAGGTCACCAAGTCCGCAGCCAAGAAGAAATGAGAAATTTTTATGG
GTCTGTCTACAATAGTTAGGGTTTATTTTCTGTCTTTTTTTATTTTCTTG
TCTGTTTTTGTTTTGCATCAATGTTTCTGCAAACAGCCCTATTTGCTTCC
ACCAGTTTGCTGTTGAGCAATTGGGATGTTATCTTTCTGCTTAACTGGCT
GTGGGTGGGTGTTCGGATTTTTATATTCTGAATTTGGTTAATAGCACTAA
ATTGAGTTGT
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