BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2225 contig Csv1_Contig2225:326..849. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 3.343e-44
Identity = 58/71 (81.69%), Postives = 68/71 (95.77%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NIN+NG+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDPLVPEIAH+YK+D+ KYE+TAR WT+KYA+
Sbjct:   78 NINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV 148

HSP 2 Score: 68.9366 bits (167), Expect = 3.343e-44
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPYAGGVF ++IHFP DYPFKPPK++F TK++HP
Sbjct:   42 ADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHP 77
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 3.343e-44
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVPEIAH YK+D+ KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM 147

HSP 2 Score: 70.0922 bits (170), Expect = 3.343e-44
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF TK++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC1_COLGL (Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletotrichum gloeosporioides GN=UBC1 PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 4.357e-44
Identity = 59/71 (83.10%), Postives = 70/71 (98.59%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL++QWSPALTISKVLLSICS+LTDPNPD+PLVPEIAH+YK+D+A+YE+TAR WT+KYA+
Sbjct:   77 NINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDEPLVPEIAHVYKTDRARYEATAREWTRKYAI 147

HSP 2 Score: 65.0846 bits (157), Expect = 4.357e-44
Identity = 25/35 (71.43%), Postives = 30/35 (85.71%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSPY+GGVF + IHFP DYPFKPPKV F T+++HP
Sbjct:   42 DSPYSGGVFFLAIHFPTDYPFKPPKVNFTTRIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 9.643e-44
Identity = 58/71 (81.69%), Postives = 68/71 (95.77%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINS+G+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDPLVPEIA +YK+DKAKYE+TA+ WT+KYA+
Sbjct:   78 NINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWTKKYAV 148

HSP 2 Score: 69.3218 bits (168), Expect = 9.643e-44
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            SDSPYAGGVF ++IHFP DYPFKPPKV F TK++HP
Sbjct:   42 SDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHP 77
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D2_XENLA (Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laevis GN=ube2d2 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.781e-43
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 2.781e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D2_RAT (Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norvegicus GN=Ube2d2 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.781e-43
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 2.781e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D2_MOUSE (Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus GN=Ube2d2 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.781e-43
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 2.781e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D2_HUMAN (Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens GN=UBE2D2 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.781e-43
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 2.781e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D2_BOVIN (Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.781e-43
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 2.781e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC4_CANAL (Ubiquitin-conjugating enzyme E2 4 OS=Candida albicans GN=UBC4 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.624e-43
Identity = 59/71 (83.10%), Postives = 67/71 (94.37%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNG+ICLDILK+QWSPALTISKVLLSICSLLTD NPDDPLVPEIAH+YK D+ KYE+TA+ WT+KYA+
Sbjct:   77 NINSNGNICLDILKDQWSPALTISKVLLSICSLLTDANPDDPLVPEIAHIYKQDRKKYEATAKEWTKKYAV 147

HSP 2 Score: 65.0846 bits (157), Expect = 3.624e-43
Identity = 25/35 (71.43%), Postives = 30/35 (85.71%), Query Frame = 1 
Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSPYAGGVF ++IHFP DYP KPPK+A  TK++HP
Sbjct:   42 DSPYAGGVFFLSIHFPTDYPLKPPKIALTTKIYHP 76
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UBC4_YEAST3.343e-4481.69Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]
UB2D4_HUMAN3.343e-4483.10Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]
UBC1_COLGL4.357e-4483.10Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletot... [more]
UBC5_YEAST9.643e-4481.69Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]
UB2D2_XENLA2.781e-4383.10Ubiquitin-conjugating enzyme E2 D2 OS=Xenopus laev... [more]
UB2D2_RAT2.781e-4383.10Ubiquitin-conjugating enzyme E2 D2 OS=Rattus norve... [more]
UB2D2_MOUSE2.781e-4383.10Ubiquitin-conjugating enzyme E2 D2 OS=Mus musculus... [more]
UB2D2_HUMAN2.781e-4383.10Ubiquitin-conjugating enzyme E2 D2 OS=Homo sapiens... [more]
UB2D2_BOVIN2.781e-4383.10Ubiquitin-conjugating enzyme E2 D2 OS=Bos taurus G... [more]
UBC4_CANAL3.624e-4383.10Ubiquitin-conjugating enzyme E2 4 OS=Candida albic... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
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