BQ623021 | Citrus Genome Database

Overview


Name	BQ623021
Unique Name	BQ623021
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	526

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2225	contig	Csv1_Contig2225:326..849.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 4.720e-43
Identity = 62/72 (86.11%), Postives = 67/72 (93.06%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAMG 325
            NI+S GSICLDILKEQWSPA T SKVLLSICSLLTDPNP+DPLVPEIAH+YK DK+KYESTA+ WTQKYAMG
Sbjct:   78 NIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149

HSP 2 Score: 62.3882 bits (150), Expect = 4.720e-43
Identity = 26/35 (74.29%), Postives = 30/35 (85.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSPY+GGVF V+I F  DYPFKPPKV F+TKV+HP
Sbjct:   43 DSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHP 77

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBCD1_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=eff PE=1 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   AR WT+KYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELAREWTRKYAM 147

HSP 2 Score: 68.5514 bits (166), Expect = 8.020e-43
Identity = 27/35 (77.14%), Postives = 31/35 (88.57%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   42 DSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D3_RAT (Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norvegicus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 8.020e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D3_PONAB (Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 8.020e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D3_MOUSE (Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 8.020e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D3_MACFA (Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fascicularis GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 8.020e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D3_HUMAN (Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens GN=UBE2D3 PE=1 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 8.020e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D2_PIG (Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.020e-43
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YK+D+ KY   +R WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147

HSP 2 Score: 68.9366 bits (167), Expect = 8.020e-43
Identity = 27/36 (75.00%), Postives = 32/36 (88.89%), Query Frame = 1

Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +DSPY GGVF +TIHFP DYPFKPPKVAF T+++HP
Sbjct:   41 NDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D1_MOUSE (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus GN=Ube2d1 PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.775e-42
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YKSDK KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM 147

HSP 2 Score: 65.855 bits (159), Expect = 1.775e-42
Identity = 25/35 (71.43%), Postives = 30/35 (85.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DS Y GGVF +T+HFP DYPFKPPK+AF TK++HP
Sbjct:   42 DSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHP 76

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2D1_HUMAN (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.775e-42
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 2

Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKYAM 322
            NINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVP+IA +YKSDK KY   AR WTQKYAM
Sbjct:   77 NINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM 147

HSP 2 Score: 65.855 bits (159), Expect = 1.775e-42
Identity = 25/35 (71.43%), Postives = 30/35 (85.71%), Query Frame = 1

Query:    4 DSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            DS Y GGVF +T+HFP DYPFKPPK+AF TK++HP
Sbjct:   42 DSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHP 76

The following BLAST results are available for this feature:

BLAST of BQ623021 vs. ExPASy Swiss-Prot

BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201


Match Name	E-value	Identity	Description
UBC12_ARATH	4.720e-43	86.11	Probable ubiquitin-conjugating enzyme E2 12 OS=Ara...	[more]
UBCD1_DROME	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi...	[more]
UB2D3_RAT	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norve...	[more]
UB2D3_PONAB	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii...	[more]
UB2D3_MOUSE	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus...	[more]
UB2D3_MACFA	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fasci...	[more]
UB2D3_HUMAN	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens...	[more]
UB2D2_PIG	8.020e-43	81.69	Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa G...	[more]
UB2D1_MOUSE	1.775e-42	83.10	Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus...	[more]
UB2D1_HUMAN	1.775e-42	83.10	Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA