BQ623021

Overview
NameBQ623021
Unique NameBQ623021
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length526
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2225 contig Csv1_Contig2225:326..849. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UB2L3_BOVIN (Ubiquitin-conjugating enzyme E2 L3 OS=Bos taurus GN=UBE2L3 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.096e-18
Identity = 25/70 (35.71%), Postives = 41/70 (58.57%), Query Frame = 2 
Query:  110 NINSNGSICLDILK-EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYESTARSWTQKY 316
            NI+  G +CL ++  E W PA    +V+ S+ +L+ DP P+ PL  ++A  Y  D+ K+   A  +T+KY
Sbjct:   78 NIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKY 147

HSP 2 Score: 53.5286 bits (127), Expect = 2.096e-18
Identity = 19/33 (57.58%), Postives = 26/33 (78.79%), Query Frame = 1 
Query:   10 PYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            PY  G F + I+FP +YPFKPPK+ F+TK++HP
Sbjct:   45 PYDKGAFRIEINFPAEYPFKPPKITFKTKIYHP 77
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2A_MOUSE (Ubiquitin-conjugating enzyme E2 A OS=Mus musculus GN=Ube2a PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.897e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 42.3578 bits (98), Expect = 2.897e-17
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F +TI F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2A_HUMAN (Ubiquitin-conjugating enzyme E2 A OS=Homo sapiens GN=UBE2A PE=2 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 2.897e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 42.3578 bits (98), Expect = 2.897e-17
Identity = 17/34 (50.00%), Postives = 23/34 (67.65%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F +TI F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_CANGA (Ubiquitin-conjugating enzyme E2 2 OS=Candida glabrata GN=UBC2 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.874e-17
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K  K++Y     E+  +SW
Sbjct:   80 NVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYIKRVKETVEKSW 149

HSP 2 Score: 43.8986 bits (102), Expect = 4.874e-17
Identity = 17/36 (47.22%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+PY  G F + + F  DYP KPP V F +++FHP
Sbjct:   44 ADTPYEDGTFRLLLEFDEDYPNKPPHVKFLSEMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2B_RAT (Ubiquitin-conjugating enzyme E2 B OS=Rattus norvegicus GN=Ube2b PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 8.279e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 40.817 bits (94), Expect = 8.279e-17
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F + I F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 8.279e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 40.817 bits (94), Expect = 8.279e-17
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F + I F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 8.279e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 40.817 bits (94), Expect = 8.279e-17
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F + I F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 8.279e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 40.817 bits (94), Expect = 8.279e-17
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F + I F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 8.279e-17
Identity = 28/59 (47.46%), Postives = 42/59 (71.19%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKYE 286
            N+ ++GSICLDIL+ +WSP   +S +L SI SLL +PNP+ P   + A +Y+ +K +YE
Sbjct:   80 NVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYE 138

HSP 2 Score: 40.817 bits (94), Expect = 8.279e-17
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = 1 
Query:    7 SPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +P+  G F + I F  +YP KPP V F +K+FHP
Sbjct:   46 TPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHP 79
[Close]
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Match: UBC2_YEAST (Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces cerevisiae GN=RAD6 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.391e-16
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 2 
Query:  110 NINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKAKY-----ESTARSW 304
            N+ +NG ICLDIL+ +W+P   ++ +L SI SL  DPNP  P   E A ++K  K++Y     E+  +SW
Sbjct:   80 NVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSW 149

HSP 2 Score: 42.3578 bits (98), Expect = 1.391e-16
Identity = 16/36 (44.44%), Postives = 24/36 (66.67%), Query Frame = 1 
Query:    1 SDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHP 108
            +D+PY  G F + + F  +YP KPP V F +++FHP
Sbjct:   44 ADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHP 79
The following BLAST results are available for this feature:
BLAST of BQ623021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
UB2L3_BOVIN2.096e-1835.71Ubiquitin-conjugating enzyme E2 L3 OS=Bos taurus G... [more]
UBE2A_MOUSE2.897e-1747.46Ubiquitin-conjugating enzyme E2 A OS=Mus musculus ... [more]
UBE2A_HUMAN2.897e-1747.46Ubiquitin-conjugating enzyme E2 A OS=Homo sapiens ... [more]
UBC2_CANGA4.874e-1741.43Ubiquitin-conjugating enzyme E2 2 OS=Candida glabr... [more]
UBE2B_RAT8.279e-1747.46Ubiquitin-conjugating enzyme E2 B OS=Rattus norveg... [more]
UBE2B_RABIT8.279e-1747.46Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c... [more]
UBE2B_MOUSE8.279e-1747.46Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ... [more]
UBE2B_HUMAN8.279e-1747.46Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ... [more]
UBE2B_BOVIN8.279e-1747.46Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN... [more]
UBC2_YEAST1.391e-1641.43Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00112 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00112 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623021 ID=BQ623021; Name=BQ623021; organism=Citrus sinensis; type=EST; length=526bp
TCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTTCC
TCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCT
TCCACCCTGAACATCAACAGCAATGGAAGCATCTGTCTTGATATCCTTAA
AGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGCTCTCTATCT
GCTCCTTGCTTACTGATCCAAATCCCGACGACCCTCTTGTACCGGAGATT
GCCCACATGTACAAGTCCGACAAGGCCAAATATGAATCCACGGCCAGGAG
CTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGATATTGCAGAA
AAAGGAGCATTTATTGAAATATGTTGTGTGTGTTGTCTATCTATTTTCTT
TAACTTGCTTTGGGGAGGGCAGACGTTGGTGTTTTAGAATGGTGTTGGGA
GGAGTTGCAAGGGCGGGACGTTTTTATGTTAATGTTGAATGTTTGTGTTT
GAAGCTTCTTCGATGGAACGTAGTTA
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