BQ623055

Overview
NameBQ623055
Unique NameBQ623055
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length715
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_RAT (Dual specificity mitogen-activated protein kinase kinase 1 OS=Rattus norvegicus GN=Map2k1 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 9.496e-17
Identity = 42/100 (42.00%), Postives = 61/100 (61.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 25.409 bits (54), Expect = 9.496e-17
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  183 KHKIMHRDVKPSNILV 198
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_RABIT (Dual specificity mitogen-activated protein kinase kinase 1 OS=Oryctolagus cuniculus GN=MAP2K1 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 9.496e-17
Identity = 42/100 (42.00%), Postives = 61/100 (61.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 25.409 bits (54), Expect = 9.496e-17
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  183 KHKIMHRDVKPSNILV 198
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_MOUSE (Dual specificity mitogen-activated protein kinase kinase 1 OS=Mus musculus GN=Map2k1 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 9.496e-17
Identity = 42/100 (42.00%), Postives = 61/100 (61.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 25.409 bits (54), Expect = 9.496e-17
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  183 KHKIMHRDVKPSNILV 198
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_HUMAN (Dual specificity mitogen-activated protein kinase kinase 1 OS=Homo sapiens GN=MAP2K1 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 9.496e-17
Identity = 42/100 (42.00%), Postives = 61/100 (61.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 25.409 bits (54), Expect = 9.496e-17
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  183 KHKIMHRDVKPSNILV 198
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K2_RAT (Dual specificity mitogen-activated protein kinase kinase 2 OS=Rattus norvegicus GN=Map2k2 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.604e-16
Identity = 40/99 (40.40%), Postives = 62/99 (62.63%), Query Frame = 2 
Query:  377 QHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            +H+ +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LK+ K IPE+ L  +   VL+GL  L  ++
Sbjct:   90 RHRPSGLIMARKLIHLEIKPAVRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEDILGKVSIAVLRGLAYLREKH 188

HSP 2 Score: 25.409 bits (54), Expect = 1.604e-16
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  187 KHQIMHRDVKPSNILV 202
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_XENLA (Dual specificity mitogen-activated protein kinase kinase 1 OS=Xenopus laevis GN=map2k1 PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.604e-16
Identity = 42/100 (42.00%), Postives = 60/100 (60.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK T    A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPTSLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGKIPEKILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 25.409 bits (54), Expect = 1.604e-16
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  183 KHKIMHRDVKPSNILV 198
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K2_MOUSE (Dual specificity mitogen-activated protein kinase kinase 2 OS=Mus musculus GN=Map2k2 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.084e-16
Identity = 40/99 (40.40%), Postives = 62/99 (62.63%), Query Frame = 2 
Query:  377 QHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            +H+ +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LK+ K IPE+ L  +   VL+GL  L  ++
Sbjct:   90 RHRPSGFIMARKLIHLEIKPAVRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEDILGKVSIAVLRGLAYLREKH 188

HSP 2 Score: 25.409 bits (54), Expect = 2.084e-16
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  187 KHQIMHRDVKPSNILV 202
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_CRIGR (Dual specificity mitogen-activated protein kinase kinase 1 OS=Cricetulus griseus GN=MAP2K1 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.086e-16
Identity = 42/100 (42.00%), Postives = 60/100 (60.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK +G   A K+I + ++ + R QI +EL++      PY+V  Y  FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGVFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 25.409 bits (54), Expect = 2.086e-16
Identity = 10/16 (62.50%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD+KPSN+++
Sbjct:  183 KHKIMHRDVKPSNILV 198
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: DSOR1_DROME (Dual specificity mitogen-activated protein kinase kinase dSOR1 OS=Drosophila melanogaster GN=Dsor1 PE=2 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 2.710e-16
Identity = 46/121 (38.02%), Postives = 65/121 (53.72%), Query Frame = 2 
Query:  290 KPSDNQLNLEDIDTXXXXXXXXXXXXQLVQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGL 652
            K    +L+ ED++               V+H  T    A K+I + V+ + ++QI +ELK+      P++V  Y +FYS+G ISI +EYMDGGSL   LK+   IPE  L  I   VLKGL
Sbjct:   76 KEKIGELSDEDLEKLGELGSGNGGVVMKVRHTHTHLIMARKLIHLEVKPAIKKQILRELKVLHECNFPHIVGFYGAFYSDGEISICMEYMDGGSLDLILKRAGRIPESILGRITLAVLKGL 196

HSP 2 Score: 26.1794 bits (56), Expect = 2.710e-16
Identity = 9/13 (69.23%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  676 IIHRDLKPSNLVI 714
            IIHRD+KPSN+++
Sbjct:  205 IIHRDVKPSNILV 217
[Close]
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Match: MP2K1_PANTR (Dual specificity mitogen-activated protein kinase kinase 1 OS=Pan troglodytes GN=MAP2K1 PE=2 SV=3)

HSP 1 Score: 82.0333 bits (201), Expect = 2.711e-16
Identity = 42/100 (42.00%), Postives = 61/100 (61.00%), Query Frame = 2 
Query:  374 VQHKWTGQFFALKVIQMNVEESARRQIAQELKINQSSQCPYVVVCYQSFYSNGAISIILEYMDGGSLADFLKKVKTIPEEYLAAICEQVLKGLLDLHHEN 673
            V HK +G   A K+I + ++ + R QI +EL++      PY+V  Y +FYS+G ISI +E+MDGGSL   LKK   IPE+ L  +   V+KGL  L  ++
Sbjct:   85 VSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKH 184

HSP 2 Score: 23.8682 bits (50), Expect = 2.711e-16
Identity = 9/16 (56.25%), Postives = 15/16 (93.75%), Query Frame = 1 
Query:  670 KH-IIHRDLKPSNLVI 714
            KH I+HRD++PSN+++
Sbjct:  183 KHKIMHRDVQPSNILV 198
The following BLAST results are available for this feature:
BLAST of BQ623055 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
MP2K1_RAT9.496e-1742.00Dual specificity mitogen-activated protein kinase ... [more]
MP2K1_RABIT9.496e-1742.00Dual specificity mitogen-activated protein kinase ... [more]
MP2K1_MOUSE9.496e-1742.00Dual specificity mitogen-activated protein kinase ... [more]
MP2K1_HUMAN9.496e-1742.00Dual specificity mitogen-activated protein kinase ... [more]
MP2K2_RAT1.604e-1640.40Dual specificity mitogen-activated protein kinase ... [more]
MP2K1_XENLA1.604e-1642.00Dual specificity mitogen-activated protein kinase ... [more]
MP2K2_MOUSE2.084e-1640.40Dual specificity mitogen-activated protein kinase ... [more]
MP2K1_CRIGR2.086e-1642.00Dual specificity mitogen-activated protein kinase ... [more]
DSOR1_DROME2.710e-1638.02Dual specificity mitogen-activated protein kinase ... [more]
MP2K1_PANTR2.711e-1642.00Dual specificity mitogen-activated protein kinase ... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00146 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00146 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623055 ID=BQ623055; Name=BQ623055; organism=Citrus sinensis; type=EST; length=715bp
ACACACTTTTCGCATTCTAAAACCCTAGAGACTTGATTTTTCATCATCAA
AGACTCTGCAACGACGACTTCGCTTCAGAAGAATCATAATAGACACAAGA
GCCCGATCATGAAGAAGGGAGGCTTAAACCCTAACGTGAAGCTCAAGCTC
TCTCTCCCTTCTTCTGAGGAAGCCTCTTTCACCAAATTTCTGACCAAGAG
CGGTACTGTTCATGGACGGTGATCTGCTCGTTAACAAAGACGGAGTTCGG
ATTGTCTCCCAGACTGAAACTGAAGCTCCACCGCCGATTAAGCCATCTGA
CAACCAGTTGAATTTGGAAGATATAGACACAATTAAAGTTGTTGGAAAGG
GAAGTGGTGGAATTGTGCAATTGGTGCAACACAAATGGACTGGCCAGTTT
TTTGCTTTAAAGGTTATTCAAATGAATGTTGAGGAGTCTGCACGAAGGCA
GATTGCTCAGGAGTTGAAAATTAATCAATCATCTCAATGTCCGTATGTTG
TCGTTTGTTATCAATCTTTCTACAGTAATGGTGCCATTTCAATTATTTTG
GAGTACATGGATGGTGGGTCTCTAGCTGATTTTCTGAAAAAAGTTAAAAC
TATTCCAGAAGAGTATCTGGCTGCCATCTGTGAGCAGGTGCTCAAGGGGT
TGTTGGATCTTCATCATGAAAACACATTATCCATAGGGATTTAAAGCCTT
CTAATTTGGTGATAA
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