BQ623098

Overview
NameBQ623098
Unique NameBQ623098
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length611
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3096 contig Csv1_Contig3096:616..1227. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALF_PLAFA (Fructose-bisphosphate aldolase OS=Plasmodium falciparum PE=1 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 9.160e-52
Identity = 95/161 (59.01%), Postives = 111/161 (68.94%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSIPCG---PSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E   QGLDGLA R  EYYK GARFAKWRTV+ I      P+ L++ E AWGLARYA+I Q N LVPIVEPEIL DG H IE    V +KV S VF  L EN V+LEG LLKP+MVT G E   K + + +   T+  L+R VPPA+PG++FLSGGQSE EA
Sbjct:  127 EKSTQGLDGLAERCKEYYKAGARFAKWRTVLVIDTAKGKPTDLSIHETAWGLARYASICQQNRLVPIVEPEILADGPHSIEVCAVVTQKVLSCVFKALQENGVLLEGALLKPNMVTAGYECTAKTTTQDVGFLTVRTLRRTVPPALPGVVFLSGGQSEEEA 287

HSP 2 Score: 50.0618 bits (118), Expect = 9.160e-52
Identity = 22/45 (48.89%), Postives = 33/45 (73.33%), Query Frame = 2 
Query:  476 STLNLNAMNQ-APNPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLN++N   P+PW ++F Y RALQ +VL TWQG+ EN   A+
Sbjct:  287 ASVNLNSINALGPHPWALTFSYGRALQASVLNTWQGKKENVAKAR 331
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALF_PLAF7 (Fructose-bisphosphate aldolase OS=Plasmodium falciparum (isolate 3D7) GN=PF14_0425 PE=3 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 9.160e-52
Identity = 95/161 (59.01%), Postives = 111/161 (68.94%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSIPCG---PSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E   QGLDGLA R  EYYK GARFAKWRTV+ I      P+ L++ E AWGLARYA+I Q N LVPIVEPEIL DG H IE    V +KV S VF  L EN V+LEG LLKP+MVT G E   K + + +   T+  L+R VPPA+PG++FLSGGQSE EA
Sbjct:  127 EKSTQGLDGLAERCKEYYKAGARFAKWRTVLVIDTAKGKPTDLSIHETAWGLARYASICQQNRLVPIVEPEILADGPHSIEVCAVVTQKVLSCVFKALQENGVLLEGALLKPNMVTAGYECTAKTTTQDVGFLTVRTLRRTVPPALPGVVFLSGGQSEEEA 287

HSP 2 Score: 50.0618 bits (118), Expect = 9.160e-52
Identity = 22/45 (48.89%), Postives = 33/45 (73.33%), Query Frame = 2 
Query:  476 STLNLNAMNQ-APNPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLN++N   P+PW ++F Y RALQ +VL TWQG+ EN   A+
Sbjct:  287 ASVNLNSINALGPHPWALTFSYGRALQASVLNTWQGKKENVAKAR 331
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALDOA_RAT (Fructose-bisphosphate aldolase A OS=Rattus norvegicus GN=Aldoa PE=1 SV=2)

HSP 1 Score: 169.088 bits (427), Expect = 2.204e-50
Identity = 93/159 (58.49%), Postives = 114/159 (71.70%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSI-PCGPSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E+  QGLDGL+ R  +Y K GA FAKWR V+ I    PS+LA+ E A  LARYA+I Q NG+VPIVEPEIL DGDH ++R   V EKV + V+  L++++V LEG LLKP+MVTPG    +K S E IA  T+T L+R VPPAVPG+ FLSGGQSE EA
Sbjct:  122 ETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSSLAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACTQKFSNEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEA 280

HSP 2 Score: 51.2174 bits (121), Expect = 2.204e-50
Identity = 23/46 (50.00%), Postives = 33/46 (71.74%), Query Frame = 2 
Query:  476 STLNLNAMNQAP--NPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLNA+N+ P   PW ++F Y RALQ + LK W G+ EN +AAQ
Sbjct:  280 ASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQ 325
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALF2_PLABA (Fructose-bisphosphate aldolase 2 OS=Plasmodium berghei (strain Anka) GN=ALDO2 PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 3.745e-50
Identity = 90/162 (55.56%), Postives = 111/162 (68.52%), Query Frame = 3 
Query:    3 NESWCQGLDGLASRSTEYYKQGARFAKWRTVVSIPCG---PSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            +E   QGLDGLA R  EYYK GARFAKWR V+ I      P+ L+++E +WGLARYA+I Q N LVPIVEPEIL DG H IE    V +KV + VF  L +N V+LEG  LKP+MVT G +  EK   + I  +T+  L+R VPPA+PG+ FLSGGQSE +A
Sbjct:  115 DEKSTQGLDGLAERCKEYYKAGARFAKWRAVLVIDPAKGKPTDLSIQEVSWGLARYASICQQNKLVPIVEPEILADGAHTIEVCATVTQKVLASVFKALHDNGVLLEGASLKPNMVTAGYDCTEKTKTDDIGFFTVRTLRRTVPPALPGVAFLSGGQSEEDA 276

HSP 2 Score: 50.0618 bits (118), Expect = 3.745e-50
Identity = 22/45 (48.89%), Postives = 33/45 (73.33%), Query Frame = 2 
Query:  476 STLNLNAMNQ-APNPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLN++N   P+PW ++F Y RALQ +VL TWQG+ EN   A+
Sbjct:  276 ASINLNSINVLGPHPWALTFSYGRALQASVLNTWQGKKENVAKAR 320
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALF2_PEA (Fructose-bisphosphate aldolase, cytoplasmic isozyme 2 OS=Pisum sativum PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 3.745e-50
Identity = 87/159 (54.72%), Postives = 117/159 (73.58%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSIPCG-PSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E+  QGLDGL +R  +YY+ GARFAKWR V+ I    PS  ++ E A+GLARYA I Q+NGLVPIVEPEIL+DG H I +   + E+V +  +  L++++V+LEG LLKP+MVTPG++   K +PE IA++T+  L+R VP AVP ++FLSGGQSE EA
Sbjct:  118 ETTTQGLDGLGARCRKYYEAGARFAKWRAVLKIGANEPSEHSIHENAYGLARYAVICQENGLVPIVEPEILVDGSHDILKCAAITERVLAATYKALSDHHVILEGTLLKPNMVTPGSD-APKVAPEVIAEHTVRALQRTVPAAVPAVVFLSGGQSEEEA 275

HSP 2 Score: 50.0618 bits (118), Expect = 3.745e-50
Identity = 23/46 (50.00%), Postives = 32/46 (69.57%), Query Frame = 2 
Query:  476 STLNLNAMNQ--APNPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLNA+NQ     PW +SF + RALQ + LK W G+ EN +AAQ
Sbjct:  275 ASVNLNAINQIKGKKPWTLSFSFGRALQQSTLKAWGGKTENVKAAQ 320
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALF2_LAMJA (Fructose-bisphosphate aldolase, non-muscle type OS=Lampetra japonica PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 6.362e-50
Identity = 92/159 (57.86%), Postives = 110/159 (69.18%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSIPCG-PSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            ES  QGLDGLA R   Y K GA FAKWR V+ I    PSALA+ E A  LARYA+I Q NGLVPIVEPEIL DGDH ++    V EKV +  +  L++++V LEG LLKP+MVT G   K + SP+ +A  T+T L+R VP AVPGI FLSGGQSE +A
Sbjct:  122 ESTTQGLDGLAERCARYKKDGADFAKWRCVLKISKNTPSALAIAENANVLARYASICQQNGLVPIVEPEILPDGDHDLKTCQYVTEKVLAATYKALSDHHVYLEGSLLKPNMVTAGQASKIRCSPQEVAMATVTALRRTVPSAVPGITFLSGGQSEEDA 280

HSP 2 Score: 53.5286 bits (127), Expect = 6.362e-50
Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = 2 
Query:  476 STLNLNAMNQAP--NPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            ++LNLNA+NQ P   PW +SF Y RALQ +VLK W G P N  AA+
Sbjct:  280 ASLNLNAINQLPLERPWALSFSYGRALQASVLKAWAGAPANIPAAK 325
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALDOA_MOUSE (Fructose-bisphosphate aldolase A OS=Mus musculus GN=Aldoa PE=1 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 8.293e-50
Identity = 93/159 (58.49%), Postives = 113/159 (71.07%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSI-PCGPSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E+  QGLDGL+ R  +Y K GA FAKWR V+ I    PSALA+ E A  LARYA+I Q NG+VPIVEPEIL DGDH ++R   V EKV + V+  L++++V LEG LLKP+MVTPG    +K S E IA  T+T L+R VPPAV G+ FLSGGQSE EA
Sbjct:  122 ETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACTQKFSNEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEA 280

HSP 2 Score: 51.2174 bits (121), Expect = 8.293e-50
Identity = 23/46 (50.00%), Postives = 33/46 (71.74%), Query Frame = 2 
Query:  476 STLNLNAMNQAP--NPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLNA+N+ P   PW ++F Y RALQ + LK W G+ EN +AAQ
Sbjct:  280 ASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQ 325
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALDOA_HUMAN (Fructose-bisphosphate aldolase A OS=Homo sapiens GN=ALDOA PE=1 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 8.293e-50
Identity = 93/159 (58.49%), Postives = 113/159 (71.07%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSI-PCGPSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E+  QGLDGL+ R  +Y K GA FAKWR V+ I    PSALA+ E A  LARYA+I Q NG+VPIVEPEIL DGDH ++R   V EKV + V+  L+++++ LEG LLKP+MVTPG    +K S E IA  T+T L+R VPPAV GI FLSGGQSE EA
Sbjct:  122 ETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEA 280

HSP 2 Score: 51.2174 bits (121), Expect = 8.293e-50
Identity = 23/46 (50.00%), Postives = 33/46 (71.74%), Query Frame = 2 
Query:  476 STLNLNAMNQAP--NPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLNA+N+ P   PW ++F Y RALQ + LK W G+ EN +AAQ
Sbjct:  280 ASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQ 325
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALDOC_PANTR (Fructose-bisphosphate aldolase C OS=Pan troglodytes GN=ALDOC PE=2 SV=3)

HSP 1 Score: 170.244 bits (430), Expect = 1.081e-49
Identity = 94/159 (59.12%), Postives = 114/159 (71.70%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSIP-CGPSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E+  QGLDGL+ R  +Y K GA FAKWR V+ I    PSALA+ E A  LARYA+I Q NG+VPIVEPEIL DGDH ++R   V EKV + V+  L++++V LEG LLKP+MVTPG     K +PE IA  T+T L+R VPPAVPG+ FLSGGQSE EA
Sbjct:  122 ETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEA 280

HSP 2 Score: 47.7506 bits (112), Expect = 1.081e-49
Identity = 21/45 (46.67%), Postives = 31/45 (68.89%), Query Frame = 2 
Query:  476 STLNLNAMNQAP--NPWHVSFFYARALQNTVLKTWQGRPENTEAA 604
            ++ NLNA+N+ P   PW ++F Y RALQ + L  W+G+ +N EAA
Sbjct:  280 ASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAEAA 324
[Close]
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Match: ALDOA_RABIT (Fructose-bisphosphate aldolase A OS=Oryctolagus cuniculus GN=ALDOA PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 1.081e-49
Identity = 92/159 (57.86%), Postives = 113/159 (71.07%), Query Frame = 3 
Query:    6 ESWCQGLDGLASRSTEYYKQGARFAKWRTVVSI-PCGPSALAVKEAAWGLARYAAISQDNGLVPIVEPEILLDGDHPIERTLEVAEKVWSEVFFYLAENNVVLEGILLKPSMVTPGAEHKEKASPETIAKYTLTMLKRRVPPAVPGIMFLSGGQSEVEA 479
            E+  QGLDGL+ R  +Y K GA FAKWR V+ I    PSALA+ E A  LARYA+I Q NG+VPIVEPEIL DGDH ++R   V EKV + V+  L+++++ LEG LLKP+MVTPG    +K S E IA  T+T L+R VPPAV G+ FLSGGQSE EA
Sbjct:  122 ETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEA 280

HSP 2 Score: 51.2174 bits (121), Expect = 1.081e-49
Identity = 23/46 (50.00%), Postives = 33/46 (71.74%), Query Frame = 2 
Query:  476 STLNLNAMNQAP--NPWHVSFFYARALQNTVLKTWQGRPENTEAAQ 607
            +++NLNA+N+ P   PW ++F Y RALQ + LK W G+ EN +AAQ
Sbjct:  280 ASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQ 325
The following BLAST results are available for this feature:
BLAST of BQ623098 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 58
Match NameE-valueIdentityDescription
ALF_PLAFA9.160e-5259.01Fructose-bisphosphate aldolase OS=Plasmodium falci... [more]
ALF_PLAF79.160e-5259.01Fructose-bisphosphate aldolase OS=Plasmodium falci... [more]
ALDOA_RAT2.204e-5058.49Fructose-bisphosphate aldolase A OS=Rattus norvegi... [more]
ALF2_PLABA3.745e-5055.56Fructose-bisphosphate aldolase 2 OS=Plasmodium ber... [more]
ALF2_PEA3.745e-5054.72Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF2_LAMJA6.362e-5057.86Fructose-bisphosphate aldolase, non-muscle type OS... [more]
ALDOA_MOUSE8.293e-5058.49Fructose-bisphosphate aldolase A OS=Mus musculus G... [more]
ALDOA_HUMAN8.293e-5058.49Fructose-bisphosphate aldolase A OS=Homo sapiens G... [more]
ALDOC_PANTR1.081e-4959.12Fructose-bisphosphate aldolase C OS=Pan troglodyte... [more]
ALDOA_RABIT1.081e-4957.86Fructose-bisphosphate aldolase A OS=Oryctolagus cu... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00189 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00189 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623098 ID=BQ623098; Name=BQ623098; organism=Citrus sinensis; type=EST; length=611bp
ACAATGAATCGTGGTGCCAAGGCTTGGATGGATTGGCATCAAGATCCACT
GAATACTACAAGCAAGGTGCTCGATTTGCTAAGTGGAGGACAGTTGTTAG
CATTCCATGTGGTCCTTCTGCTCTGGCTGTGAAGGAAGCTGCATGGGGCC
TTGCACGATATGCTGCTATTTCTCAGGATAATGGACTTGTGCCTATAGTG
GAACCCGAGATCCTTCTTGATGGGGACCACCCAATTGAGAGGACCCTTGA
AGTGGCAGAAAAGGTCTGGTCAGAAGTCTTTTTCTACTTGGCAGAAAACA
ATGTCGTTCTTGAGGGAATTCTACTTAAACCCAGCATGGTCACGCCAGGT
GCTGAACACAAGGAGAAGGCCTCTCCAGAGACCATTGCTAAATATACACT
TACAATGCTCAAGAGGAGAGTACCCCCTGCAGTCCCAGGAATCATGTTTT
TGTCTGGAGGACAATCAGAAGTGGAAGCACATTGAACTTGAATGCGATGA
ACCAAGCCCCCAACCCCTGGCACGTGTCCTTTTTTTATGCCCGTGCACTT
CAAAACACTGTTCTTAAAACATGGCAAGGTCGTCCTGAGAATACTGAAGC
TGCCCAAAGTC
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