BQ623598

Overview
NameBQ623598
Unique NameBQ623598
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length636
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10924 contig Csv1_Contig10924:235..869. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_ASPOR (ATP-dependent RNA helicase eIF4A OS=Aspergillus oryzae GN=tif1 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 3.243e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  270 KEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQSQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 421

HSP 2 Score: 24.6386 bits (52), Expect = 3.243e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEERQ 84
            ELTL+GI Q+Y  VE+ +
Sbjct:  255 ELTLEGIKQFYIAVEKEE 272
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: FAL1_MAGGR (ATP-dependent RNA helicase FAL1 OS=Magnaporthe grisea GN=FAL1 PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 3.245e-30
Identity = 63/152 (41.45%), Postives = 93/152 (61.18%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L  L+  L I Q++IFCN+  +V+ L  K+ E  ++   +H  M Q  R+ +  DFR G  R L+ TD++ RGID+Q V++VIN+D P N E Y+HR+GRSGRFG  G+A+N +T ED   L  IE    T+I ++P ++   I
Sbjct:  249 KEDWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGDMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPSNRENYIHRIGRSGRFGRKGVAINFVTSEDVRILRDIELYYSTQIDEMPMNVADLI 400

HSP 2 Score: 25.0238 bits (53), Expect = 3.245e-30
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:   28 DELTLKGITQYYAFVEE 78
            DELTL+G+ QY+  +E+
Sbjct:  233 DELTLEGLKQYFIAIEK 249
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_ASPCL (ATP-dependent RNA helicase eIF4A OS=Aspergillus clavatus GN=tif1 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 3.246e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  247 KEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQSQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398

HSP 2 Score: 24.6386 bits (52), Expect = 3.246e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEERQ 84
            ELTL+GI Q+Y  VE+ +
Sbjct:  232 ELTLEGIKQFYIAVEKEE 249
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: FAL1_BOTFB (ATP-dependent RNA helicase fal1 OS=Botryotinia fuckeliana (strain B05.10) GN=fal1 PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 3.246e-30
Identity = 62/148 (41.89%), Postives = 93/148 (62.84%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHI 517
            K+  K   L  L+  L I Q++IFCN+  +V+ L  K+ E  ++   +H +M Q  R+ +  DFR G  R L+ TD++ RGID+Q V++VIN+D P N E Y+HR+GRSGRFG  G+A+N +T ED   L  IE    T+I ++P ++
Sbjct:  247 KEDWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGEMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPVNRENYIHRIGRSGRFGRKGVAINFVTSEDVRILRDIELYYSTQIDEMPMNV 394

HSP 2 Score: 25.409 bits (54), Expect = 3.246e-30
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:   28 DELTLKGITQYYAFVEE 78
            DELTL+G+ QY+  VE+
Sbjct:  231 DELTLEGLKQYFIAVEK 247
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_ASPTN (ATP-dependent RNA helicase eIF4A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=tif1 PE=3 SV=2)

HSP 1 Score: 127.487 bits (319), Expect = 3.246e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  245 KEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQSQRDIIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 396

HSP 2 Score: 24.6386 bits (52), Expect = 3.246e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEERQ 84
            ELTL+GI Q+Y  VE+ +
Sbjct:  230 ELTLEGIKQFYIAVEKEE 247
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_ASPNC (ATP-dependent RNA helicase eIF4A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN=tif1 PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 4.226e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  247 KEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQGQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398

HSP 2 Score: 24.6386 bits (52), Expect = 4.226e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEERQ 84
            ELTL+GI Q+Y  VE+ +
Sbjct:  232 ELTLEGIKQFYIAVEKEE 249
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_EMENI (ATP-dependent RNA helicase eIF4A OS=Emericella nidulans GN=tif1 PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 5.498e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  270 KEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 421

HSP 2 Score: 24.6386 bits (52), Expect = 5.498e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEERQ 84
            ELTL+GI Q+Y  VE+ +
Sbjct:  255 ELTLEGIKQFYIAVEKEE 272
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_BOTFB (ATP-dependent RNA helicase eIF4A OS=Botryotinia fuckeliana (strain B05.10) GN=tif1 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 5.503e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  247 KEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMDQGQRDLIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398

HSP 2 Score: 23.8682 bits (50), Expect = 5.503e-30
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEE 78
            ELTL+GI Q+Y  VE+
Sbjct:  232 ELTLEGIKQFYIAVEK 247
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_NEOFI (ATP-dependent RNA helicase eIF4A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=tif1 PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 5.503e-30
Identity = 60/152 (39.47%), Postives = 96/152 (63.16%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+T   ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  247 KEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398

HSP 2 Score: 24.6386 bits (52), Expect = 5.503e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 1 
Query:   31 ELTLKGITQYYAFVEERQ 84
            ELTL+GI Q+Y  VE+ +
Sbjct:  232 ELTLEGIKQFYIAVEKEE 249
[Close]
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Match: IF4A_CHAGB (ATP-dependent RNA helicase eIF4A OS=Chaetomium globosum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.503e-30
Identity = 59/152 (38.82%), Postives = 95/152 (62.50%), Query Frame = 2 
Query:   74 KKGRKSTCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPKNSETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAI 529
            K+  K   L+ L+  + I Q++IFCN+  +V+ L  K+    ++   +H  M Q  R+ +  +FR+G+ R L+ TDL  RGID+Q V++VIN+D P N E Y+HR+GR GRFG  G+A+N +T +D   +  IEQ   T+I+++P ++   I
Sbjct:  246 KEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLQARDFTVSAMHGDMDQTQRDLIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 397

HSP 2 Score: 26.1794 bits (56), Expect = 5.503e-30
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:   28 DELTLKGITQYYAFVEE 78
            DELTL+GI Q+Y  VE+
Sbjct:  230 DELTLEGIKQFYIAVEK 246
The following BLAST results are available for this feature:
BLAST of BQ623598 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_ASPOR3.243e-3039.47ATP-dependent RNA helicase eIF4A OS=Aspergillus or... [more]
FAL1_MAGGR3.245e-3041.45ATP-dependent RNA helicase FAL1 OS=Magnaporthe gri... [more]
IF4A_ASPCL3.246e-3039.47ATP-dependent RNA helicase eIF4A OS=Aspergillus cl... [more]
FAL1_BOTFB3.246e-3041.89ATP-dependent RNA helicase fal1 OS=Botryotinia fuc... [more]
IF4A_ASPTN3.246e-3039.47ATP-dependent RNA helicase eIF4A OS=Aspergillus te... [more]
IF4A_ASPNC4.226e-3039.47ATP-dependent RNA helicase eIF4A OS=Aspergillus ni... [more]
IF4A_EMENI5.498e-3039.47ATP-dependent RNA helicase eIF4A OS=Emericella nid... [more]
IF4A_BOTFB5.503e-3039.47ATP-dependent RNA helicase eIF4A OS=Botryotinia fu... [more]
IF4A_NEOFI5.503e-3039.47ATP-dependent RNA helicase eIF4A OS=Neosartorya fi... [more]
IF4A_CHAGB5.503e-3038.82ATP-dependent RNA helicase eIF4A OS=Chaetomium glo... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00689 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00689 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623598 ID=BQ623598; Name=BQ623598; organism=Citrus sinensis; type=EST; length=636bp
CAAAAACCATATGTTATTAATCTGATGGATGAGCTTACTCTCAAGGGAAT
TACCCAATACTATGCTTTTGTTGAAGAAAGGCAGAAAGTCCACTTGCCTC
AACACACTTTTCTCTAAGTTGCAAATTAATCAATCTATCATTTTCTGCAA
TTCGGTGAATCGGGTTGAACTCCTTGCCAAGAAAATTACAGAACTTGGGT
ACTCTTGTTTTTATATCCATGCAAAGATGCTGCAAGACCATCGCAACAGA
GTATTTCATGACTTCCGCAATGGTGCATGCAGGAATCTTGTTTGTACTGA
CCTATTTACGAGGGGTATAGACATTCAAGCAGTGAATGTTGTCATTAACT
TTGATTTTCCTAAGAACTCGGAGACATATCTGCACAGAGTTGGTCGATCT
GGAAGGTTTGGACACCTTGGATTAGCTGTGAATTTGATTACTTACGAGGA
CCGCTTCAACTTGTATAGGATTGAGCAAGAACTCGGGACTGAAATTAAGC
AGATACCTCCGCATATTGATCAGGCAATTTACTGCAGGTGATTTATGGCC
GGTATCTTGCTATATAGCCAATGGTGGTAGCAATGGATTCCTGAGGCTGT
TGATTCAAGGCACTTGGCTCTGCAGCTGAGGAAGCA
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