CB250356

Overview
NameCB250356
Unique NameCB250356
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length464
Libraries
Library NameType
Mature fruit abscission zone cDNA subtraction librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7556 contig Csv1_Contig7556:35..498. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_SALTY (Thioredoxin-1 OS=Salmonella typhimurium GN=trxA PE=3 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 2 
Query:   92 LADDVVVLTEDNFEKEVGQDRGA-LVEFYAPWCGHCKKLAPEYERLGASFKKAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            ++D ++ LT+D+F+ +V +  GA LV+F+A WCG CK +AP  + +   ++    + + K++ D++     KYG++G PT+  F  G +   K  G  S   L E+++
Sbjct:    1 MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQ--GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV-GALSKGQLKEFLD 105
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_SALTI (Thioredoxin-1 OS=Salmonella typhi GN=trxA PE=3 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 2 
Query:   92 LADDVVVLTEDNFEKEVGQDRGA-LVEFYAPWCGHCKKLAPEYERLGASFKKAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            ++D ++ LT+D+F+ +V +  GA LV+F+A WCG CK +AP  + +   ++    + + K++ D++     KYG++G PT+  F  G +   K  G  S   L E+++
Sbjct:    1 MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQ--GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV-GALSKGQLKEFLD 105
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_HELPY (Thioredoxin OS=Helicobacter pylori GN=trxA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 40/103 (38.83%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:  107 VVLTEDNFEKEVGQDRGALVEFYAPWCGHCKKLAPEYERLGASFK-KAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            + LTE+NFE  + +   ALV+F+APWCG CK L+P  + L + ++ KAK   I KV+ DE + L +K+G++  PT+  F K      +  G ++  AL E +N
Sbjct:    5 IELTEENFESTIKKGV-ALVDFWAPWCGPCKMLSPVIDELASEYEGKAK---ICKVNTDEQEELSAKFGIRSIPTL-LFTKDGEVVHQLVGVQTKVALKEQLN 102
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_HELPJ (Thioredoxin OS=Helicobacter pylori J99 GN=trxA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 40/103 (38.83%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:  107 VVLTEDNFEKEVGQDRGALVEFYAPWCGHCKKLAPEYERLGASFK-KAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            + LTE+NFE  + +   ALV+F+APWCG CK L+P  + L + ++ KAK   I KV+ DE + L +K+G++  PT+  F K      +  G ++  AL E +N
Sbjct:    5 IELTEENFESTIKKGV-ALVDFWAPWCGPCKMLSPVIDELASEYEGKAK---ICKVNTDEQEELSAKFGIRSIPTL-LFTKDGEVVHQLVGVQTKVALKEQLN 102
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_ECOLI (Thioredoxin-1 OS=Escherichia coli (strain K12) GN=trxA PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 2 
Query:   92 LADDVVVLTEDNFEKEVGQDRGA-LVEFYAPWCGHCKKLAPEYERLGASFKKAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            ++D ++ LT+D+F+ +V +  GA LV+F+A WCG CK +AP  + +   ++    + + K++ D++     KYG++G PT+  F  G +   K  G  S   L E+++
Sbjct:    1 MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQ--GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV-GALSKGQLKEFLD 105
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_ECOL6 (Thioredoxin-1 OS=Escherichia coli O6 GN=trxA PE=3 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 2 
Query:   92 LADDVVVLTEDNFEKEVGQDRGA-LVEFYAPWCGHCKKLAPEYERLGASFKKAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            ++D ++ LT+D+F+ +V +  GA LV+F+A WCG CK +AP  + +   ++    + + K++ D++     KYG++G PT+  F  G +   K  G  S   L E+++
Sbjct:    1 MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQ--GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV-GALSKGQLKEFLD 105
[Close]
BLAST of CB250356 vs. ExPASy Swiss-Prot
Match: THIO_ECO57 (Thioredoxin-1 OS=Escherichia coli O157:H7 GN=trxA PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 7.555e-11
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 2 
Query:   92 LADDVVVLTEDNFEKEVGQDRGA-LVEFYAPWCGHCKKLAPEYERLGASFKKAKSVLIGKVDCDEHKSLCSKYGVQGYPTIQWFPKGSLEPKKYEGPRSTEALAEYVN 412
            ++D ++ LT+D+F+ +V +  GA LV+F+A WCG CK +AP  + +   ++    + + K++ D++     KYG++G PT+  F  G +   K  G  S   L E+++
Sbjct:    1 MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQ--GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV-GALSKGQLKEFLD 105
The following BLAST results are available for this feature:
BLAST of CB250356 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 87
Match NameE-valueIdentityDescription
THIO_SALTY7.555e-1131.48Thioredoxin-1 OS=Salmonella typhimurium GN=trxA PE... [more]
THIO_SALTI7.555e-1131.48Thioredoxin-1 OS=Salmonella typhi GN=trxA PE=3 SV=... [more]
THIO_HELPY7.555e-1138.83Thioredoxin OS=Helicobacter pylori GN=trxA PE=3 SV... [more]
THIO_HELPJ7.555e-1138.83Thioredoxin OS=Helicobacter pylori J99 GN=trxA PE=... [more]
THIO_ECOLI7.555e-1131.48Thioredoxin-1 OS=Escherichia coli (strain K12) GN=... [more]
THIO_ECOL67.555e-1131.48Thioredoxin-1 OS=Escherichia coli O6 GN=trxA PE=3 ... [more]
THIO_ECO577.555e-1131.48Thioredoxin-1 OS=Escherichia coli O157:H7 GN=trxA ... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionEST0150 Mature fruit abscission zone cDNA subtraction library Citrus sinensis cDNA clone MFAZ219 similar to protein disulfide isomerase (acc# NP_182269), mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB250356 ID=CB250356; Name=CB250356; organism=Citrus sinensis; type=EST; length=464bp
CACGGGGGGAAAAAAAAAAAGAAAGAGAGAAATGGAGAGGTATCAGATCT
GGCTAGCTCTAGGCACTTTAACCTTGTTCTTCGTATCAGCTCTAGCGGAC
GATGTCGTTGTGCTAACAGAAGACAACTTCGAGAAGGAGGTTGGTCAGGA
CAGGGGAGCTCTTGTTGAGTTTTACGCTCCTTGGTGTGGCCACTGTAAAA
AGCTTGCTCCAGAGTATGAGAGACTTGGTGCAAGCTTTAAGAAAGCAAAG
TCTGTCTTGATTGGAAAGGTGGACTGTGACGAGCATAAGAGCTTATGCAG
CAAATATGGTGTTCAAGGATACCCAACAATTCAATGGTTTCCTAAAGGTT
CTTTGGAACCCAAAAAGTATGAAGGGCCACGATCTACTGAAGCCCTTGCT
GAGTATGTGAATAATGAAGGAGGAACCAATGTGAAGATAGCTGCAGTCCC
ATCCAATGTGGTGG
back to top