CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_XENLA (Heat shock 70 kDa protein OS=Xenopus laevis PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.131e-64
Identity = 68/87 (78.16%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHFV+EFKRK+KKDI  N RALRRLRTAC+RAKRTLSS++Q +IEIDSL+EGIDFYT ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  237 RMVNHFVEEFKRKHKKDIGQNKRALRRLRTACDRAKRTLSSSSQASIEIDSLFEGIDFYTAITRARFEELCSDLFRGTLEPVEKALR 323

HSP 2 Score: 131.724 bits (330), Expect = 1.131e-64
Identity = 68/110 (61.82%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DKS +H++VLVGGSTRIPKVQ+LLQDFFNG+EL KSINPDE           IL G+ +E VQD             ETAGGVMTVLI RNTTIPTK+ Q F+TY
Sbjct:  323 RDAKLDKSQIHEIVLVGGSTRIPKVQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTVLIKRNTTIPTKQTQSFTTY 432

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP76_HUMAN (Heat shock 70 kDa protein 6 OS=Homo sapiens GN=HSPA6 PE=1 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.923e-64
Identity = 65/87 (74.71%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF++EF+RK+ KD+SGN RALRRLRTACERAKRTLSS+ Q T+EIDSL+EG+DFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVNHFMEEFRRKHGKDLSGNKRALRRLRTACERAKRTLSSSTQATLEIDSLFEGVDFYTSITRARFEELCSDLFRSTLEPVEKALR 324

HSP 2 Score: 130.954 bits (328), Expect = 1.923e-64
Identity = 68/110 (61.82%), Postives = 76/110 (69.09%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK+ +HDVVLVGGSTRIPKVQ+LLQDFFNGKEL KSINPDE           +L G+  EKVQD             ETAGGVMT LI RN TIPTK+ Q F+TY
Sbjct:  324 RDAKLDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7D_DROME (Heat shock 70 kDa protein cognate 4 OS=Drosophila melanogaster GN=Hsc70-4 PE=1 SV=3)

HSP 1 Score: 137.117 bits (344), Expect = 2.507e-64
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V HFVQEFKRK+KKD++ N RALRRLRTACERAKRTLSS+ Q +IEIDSL+EG DFYT+ITRARFEELN DLFR  M+PVEK  R
Sbjct:  236 RLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGTDFYTSITRARFEELNADLFRSTMDPVEKALR 322

HSP 2 Score: 129.798 bits (325), Expect = 2.507e-64
Identity = 67/110 (60.91%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DKS +HD+VLVGGSTRIPKVQ+LLQD FNGKEL KSINPDE           IL G+ +++VQD             ETAGGVM+VLI RNTTIPTK+ Q F+TY
Sbjct:  322 RDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71L_MACFA (Heat shock 70 kDa protein 1-like OS=Macaca fascicularis GN=HSPA1L PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.507e-64
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALR 324

HSP 2 Score: 131.724 bits (330), Expect = 2.507e-64
Identity = 66/110 (60.00%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMT LI RN+TIPTK+ Q+F+TY
Sbjct:  324 RDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71L_HUMAN (Heat shock 70 kDa protein 1-like OS=Homo sapiens GN=HSPA1L PE=1 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 2.507e-64
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q  +EIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  238 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALR 324

HSP 2 Score: 131.724 bits (330), Expect = 2.507e-64
Identity = 66/110 (60.00%), Postives = 79/110 (71.82%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQD+FNG++L KSINPDE           IL G+ +EKVQD             ETAGGVMT LI RN+TIPTK+ Q+F+TY
Sbjct:  324 RDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTY 433

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP7C_ICTPU (Heat shock cognate 71 kDa protein OS=Ictalurus punctatus GN=hsc70 PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 4.263e-64
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMVNHF+ EFKRK+KKDIS N RA+RRL TACERAKRTLSS+ Q +IEIDSLYEG+DFYT+ITRARFEELN DLFR  ++PVEK  R
Sbjct:  236 RMVNHFIAEFKRKHKKDISDNKRAVRRLATACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLDPVEKALR 322

HSP 2 Score: 128.257 bits (321), Expect = 4.263e-64
Identity = 67/110 (60.91%), Postives = 76/110 (69.09%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ VHD+VLVGGSTRIPK+++LLQDFFNGKEL KSINPDE              G+ +E VQD             ETAGGVMTVLI RNTTIPTK+ Q F+TY
Sbjct:  322 RDAKMDKAQVHDIVLVGGSTRIPKMEKLLQDFFNGKELNKSINPDEAVAYGAAVQAESSLGDKSENVQDLVLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_YEAST (Heat shock protein SSA2 OS=Saccharomyces cerevisiae GN=SSA2 PE=1 SV=3)

HSP 1 Score: 142.124 bits (357), Expect = 4.264e-64
Identity = 69/87 (79.31%), Postives = 79/87 (90.80%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF+QEFKRKNKKD+S N RALRRLRTACERAKRTLSS+AQT++EIDSL+EGIDFYT+ITRARFEEL  DLFR  ++PVEK  R
Sbjct:  233 RLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLR 319

HSP 2 Score: 124.02 bits (310), Expect = 4.264e-64
Identity = 64/110 (58.18%), Postives = 76/110 (69.09%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DKS V ++VLVGGSTRIPKVQ+L+ D+FNGKE  +SINPDE           IL+G+ + K QD             ETAGGVMT LIPRN+TIPTKK +VFSTY
Sbjct:  319 RDAKLDKSQVDEIVLVGGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSEVFSTY 428

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_YEAST (Heat shock protein SSA1 OS=Saccharomyces cerevisiae GN=SSA1 PE=1 SV=4)

HSP 1 Score: 142.124 bits (357), Expect = 5.559e-64
Identity = 69/87 (79.31%), Postives = 79/87 (90.80%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF+QEFKRKNKKD+S N RALRRLRTACERAKRTLSS+AQT++EIDSL+EGIDFYT+ITRARFEEL  DLFR  ++PVEK  R
Sbjct:  233 RLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLR 319

HSP 2 Score: 123.635 bits (309), Expect = 5.559e-64
Identity = 63/110 (57.27%), Postives = 76/110 (69.09%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DKS V ++VLVGGSTRIPKVQ+L+ D+FNGKE  +SINPDE           IL+G+ + K QD             ETAGGVMT LIPRN+TIPTKK ++FSTY
Sbjct:  319 RDAKLDKSQVDEIVLVGGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSEIFSTY 428

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_PONAB (Heat shock 70 kDa protein 1 OS=Pongo abelii GN=HSPA1 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 5.559e-64
Identity = 67/87 (77.01%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALR 322

HSP 2 Score: 129.028 bits (323), Expect = 5.559e-64
Identity = 65/110 (59.09%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK+ Q+F+TY
Sbjct:  322 RDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_HUMAN (Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN=HSPA1A PE=1 SV=5)

HSP 1 Score: 136.732 bits (343), Expect = 5.559e-64
Identity = 67/87 (77.01%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  236 RLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALR 322

HSP 2 Score: 129.028 bits (323), Expect = 5.559e-64
Identity = 65/110 (59.09%), Postives = 78/110 (70.91%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK+ Q+F+TY
Sbjct:  322 RDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTY 431

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP70_XENLA	1.131e-64	78.16	Heat shock 70 kDa protein OS=Xenopus laevis PE=2 S...	[more]
HSP76_HUMAN	1.923e-64	74.71	Heat shock 70 kDa protein 6 OS=Homo sapiens GN=HSP...	[more]
HSP7D_DROME	2.507e-64	77.01	Heat shock 70 kDa protein cognate 4 OS=Drosophila ...	[more]
HS71L_MACFA	2.507e-64	77.01	Heat shock 70 kDa protein 1-like OS=Macaca fascicu...	[more]
HS71L_HUMAN	2.507e-64	77.01	Heat shock 70 kDa protein 1-like OS=Homo sapiens G...	[more]
HSP7C_ICTPU	4.263e-64	77.01	Heat shock cognate 71 kDa protein OS=Ictalurus pun...	[more]
HSP72_YEAST	4.264e-64	79.31	Heat shock protein SSA2 OS=Saccharomyces cerevisia...	[more]
HSP71_YEAST	5.559e-64	79.31	Heat shock protein SSA1 OS=Saccharomyces cerevisia...	[more]
HSP71_PONAB	5.559e-64	77.01	Heat shock 70 kDa protein 1 OS=Pongo abelii GN=HSP...	[more]
HSP71_HUMAN	5.559e-64	77.01	Heat shock 70 kDa protein 1A/1B OS=Homo sapiens GN...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC