CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_CANAL (Heat shock protein SSA1 OS=Candida albicans GN=SSA1 PE=1 SV=2)

HSP 1 Score: 138.272 bits (347), Expect = 2.732e-63
Identity = 69/84 (82.14%), Postives = 76/84 (90.48%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VN F+QEFKRKNKKDIS N RALRRLRTACERAKRTLSS+AQT+IEIDSLYEGIDFYT+ITRARFEEL  DLFR  ++PV K
Sbjct:  235 RLVNFFIQEFKRKNKKDISTNQRALRRLRTACERAKRTLSSSAQTSIEIDSLYEGIDFYTSITRARFEELCADLFRSTLDPVGK 318

HSP 2 Score: 125.176 bits (313), Expect = 2.732e-63
Identity = 63/109 (57.80%), Postives = 75/109 (68.81%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAK+DKS V ++VLVGGSTRIPK+Q+L+ DFFNGKEL KSINPDE           IL+G+ + K QD             ETAGG+MT LIPRN+TIPTKK + FSTY
Sbjct:  322 DAKIDKSQVEEIVLVGGSTRIPKIQKLVSDFFNGKELNKSINPDEAVAYGAAVQAAILTGDTSSKTQDILLLDVAPLSLGIETAGGIMTKLIPRNSTIPTKKSETFSTY 430

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71B_MOUSE (Heat shock 70 kDa protein 1B OS=Mus musculus GN=Hspa1b PE=1 SV=3)

HSP 1 Score: 134.035 bits (336), Expect = 3.563e-63
Identity = 66/87 (75.86%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  236 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALR 322

HSP 2 Score: 129.028 bits (323), Expect = 3.563e-63
Identity = 66/110 (60.00%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK+ Q F+TY
Sbjct:  322 RDAKMDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HS71A_MOUSE (Heat shock 70 kDa protein 1A OS=Mus musculus GN=Hspa1a PE=1 SV=2)

HSP 1 Score: 134.035 bits (336), Expect = 3.563e-63
Identity = 66/87 (75.86%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  236 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALR 322

HSP 2 Score: 129.028 bits (323), Expect = 3.563e-63
Identity = 66/110 (60.00%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAKMDK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK+ Q F+TY
Sbjct:  322 RDAKMDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_PICAN (Heat shock protein 70 1 OS=Pichia angusta GN=HSA1 PE=1 SV=2)

HSP 1 Score: 137.117 bits (344), Expect = 4.645e-63
Identity = 67/87 (77.01%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHF  EFKRK KKD++ N RALRRLRTACERAKRTLSS+AQT++EIDSLYEGIDFYT+ITRARFEEL  DLFR  ++PVEK  R
Sbjct:  236 RLVNHFANEFKRKYKKDLTTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLYEGIDFYTSITRARFEELCQDLFRSTLDPVEKVMR 322

HSP 2 Score: 125.561 bits (314), Expect = 4.645e-63
Identity = 64/110 (58.18%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RD K+DKS V ++VLVGGSTRIPK+Q+L+ DFFNGKE  KSINPDE           IL+G+ + K QD             ETAGGVMT LIPRNTTIPTKK ++FSTY
Sbjct:  322 RDGKLDKSQVAEIVLVGGSTRIPKIQKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILTGDTSSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNTTIPTKKSEIFSTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_TRIRU (Heat shock 70 kDa protein OS=Trichophyton rubrum GN=HSP70 PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 6.057e-63
Identity = 71/87 (81.61%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFV EFKRKNKKD+S N RALRRLRTACERAKRTLSS AQT+IEIDSLYEG+DFYT+ITRARFEEL  DLFR  MEPVE+  R
Sbjct:  234 RLVNHFVNEFKRKNKKDLSTNARALRRLRTACERAKRTLSSAAQTSIEIDSLYEGVDFYTSITRARFEELCQDLFRSTMEPVERVLR 320

HSP 2 Score: 119.013 bits (297), Expect = 6.057e-63
Identity = 64/114 (56.14%), Postives = 76/114 (66.67%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEK-VQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  RDAK+DKSSVH++VLVGGSTRIPK+Q+++ DFFNGKE  KSINPDE           ILSG+ + K   +             ETAGGVMT LI RNTTIPTKK + FST+
Sbjct:  317 RVLRDAKIDKSSVHEIVLVGGSTRIPKIQKMVSDFFNGKEPNKSINPDEGVAYGAAVQAAILSGDTSSKSTNEILLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSETFSTF 430

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_CANAL (Heat shock protein SSA2 OS=Candida albicans GN=SSA2 PE=1 SV=3)

HSP 1 Score: 140.969 bits (354), Expect = 6.057e-63
Identity = 70/84 (83.33%), Postives = 77/84 (91.67%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VN F+QEFKRKNKKDIS N RALRRLRTACERAKRTLSS+AQT+IEIDSLYEGIDFYT+ITRARFEEL  DLFR  +EPV+K
Sbjct:  234 RLVNFFIQEFKRKNKKDISTNQRALRRLRTACERAKRTLSSSAQTSIEIDSLYEGIDFYTSITRARFEELCADLFRSTLEPVDK 317

HSP 2 Score: 121.324 bits (303), Expect = 6.057e-63
Identity = 62/109 (56.88%), Postives = 74/109 (67.89%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAK+DKS V ++VLVGGSTRIPKVQ+L+ D+FNGKE  +SINPDE           ILSG+ + K QD             ETAGG+MT LIPRN+TIPTKK + FSTY
Sbjct:  321 DAKIDKSKVDEIVLVGGSTRIPKVQKLVSDYFNGKEPNRSINPDEAVAYGAAVQAAILSGDTSSKTQDLLLLDVAPLSLGIETAGGIMTKLIPRNSTIPTKKSETFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_RAT (Heat shock 70 kDa protein 1A/1B OS=Rattus norvegicus GN=Hspa1a PE=2 SV=2)

HSP 1 Score: 134.035 bits (336), Expect = 7.898e-63
Identity = 66/87 (75.86%), Postives = 77/87 (88.51%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+V+HFV+EFKRK+KKDIS N RA+RRLRTACERAKRTLSS+ Q ++EIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK  R
Sbjct:  236 RLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALR 322

HSP 2 Score: 127.872 bits (320), Expect = 7.898e-63
Identity = 65/110 (59.09%), Postives = 77/110 (70.00%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK+ +HD+VLVGGSTRIPKVQ+LLQDFFNG++L KSINPDE           IL G+ +E VQD             ETAGGVMT LI RN+TIPTK+ Q F+TY
Sbjct:  322 RDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_CAPHI (Heat shock-related 70 kDa protein 2 OS=Capra hircus GN=HSPA2 PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.030e-62
Identity = 66/87 (75.86%), Postives = 76/87 (87.36%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMV+H  +EFKRK+KKDI+ N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEG+DFYT+ITRARFEELN DLFR  +EPVEK  R
Sbjct:  239 RMVSHLAEEFKRKHKKDIAPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRVTLEPVEKALR 325

HSP 2 Score: 125.946 bits (315), Expect = 1.030e-62
Identity = 65/110 (59.09%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK  + ++VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           IL G+ +E VQD             ETAGGVMT LI RNTTIPTK+ Q F+TY
Sbjct:  325 RDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTY 434

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_HUMAN (Heat shock-related 70 kDa protein 2 OS=Homo sapiens GN=HSPA2 PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 1.343e-62
Identity = 66/87 (75.86%), Postives = 75/87 (86.21%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMV+H  +EFKRK+KKDI  N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEG+DFYT+ITRARFEELN DLFR  +EPVEK  R
Sbjct:  239 RMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALR 325

HSP 2 Score: 125.946 bits (315), Expect = 1.343e-62
Identity = 65/110 (59.09%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK  + ++VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           IL G+ +E VQD             ETAGGVMT LI RNTTIPTK+ Q F+TY
Sbjct:  325 RDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTY 434

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_RAT (Heat shock-related 70 kDa protein 2 OS=Rattus norvegicus GN=Hspa2 PE=2 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 1.343e-62
Identity = 66/87 (75.86%), Postives = 75/87 (86.21%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            RMV+H  +EFKRK+KKDI  N RA+RRLRTACERAKRTLSS+ Q +IEIDSLYEG+DFYT+ITRARFEELN DLFR  +EPVEK  R
Sbjct:  239 RMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALR 325

HSP 2 Score: 125.946 bits (315), Expect = 1.343e-62
Identity = 65/110 (59.09%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            RDAK+DK  + ++VLVGGSTRIPK+Q+LLQDFFNGKEL KSINPDE           IL G+ +E VQD             ETAGGVMT LI RNTTIPTK+ Q F+TY
Sbjct:  325 RDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTY 434

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP71_CANAL	2.732e-63	82.14	Heat shock protein SSA1 OS=Candida albicans GN=SSA...	[more]
HS71B_MOUSE	3.563e-63	75.86	Heat shock 70 kDa protein 1B OS=Mus musculus GN=Hs...	[more]
HS71A_MOUSE	3.563e-63	75.86	Heat shock 70 kDa protein 1A OS=Mus musculus GN=Hs...	[more]
HSP71_PICAN	4.645e-63	77.01	Heat shock protein 70 1 OS=Pichia angusta GN=HSA1 ...	[more]
HSP70_TRIRU	6.057e-63	81.61	Heat shock 70 kDa protein OS=Trichophyton rubrum G...	[more]
HSP72_CANAL	6.057e-63	83.33	Heat shock protein SSA2 OS=Candida albicans GN=SSA...	[more]
HSP71_RAT	7.898e-63	75.86	Heat shock 70 kDa protein 1A/1B OS=Rattus norvegic...	[more]
HSP72_CAPHI	1.030e-62	75.86	Heat shock-related 70 kDa protein 2 OS=Capra hircu...	[more]
HSP72_HUMAN	1.343e-62	75.86	Heat shock-related 70 kDa protein 2 OS=Homo sapien...	[more]
HSP72_RAT	1.343e-62	75.86	Heat shock-related 70 kDa protein 2 OS=Rattus norv...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC