CB417297 | Citrus Genome Database

Overview


Name	CB417297
Unique Name	CB417297
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	590

Libraries


Library Name	Type
Mature peel (flavedo) cDNA subtraction library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13846	contig	Csv1_Contig13846:800..1391.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP74_YEAST (Heat shock protein SSA4 OS=Saccharomyces cerevisiae GN=SSA4 PE=1 SV=3)

HSP 1 Score: 129.413 bits (324), Expect = 6.535e-59
Identity = 64/84 (76.19%), Postives = 74/84 (88.10%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VN   +EFKRKNKKD++ N R+LRRLRTA ERAKRTLSS+AQT+IEIDSL+EGIDFYT+ITRARFEEL  DLFR  +EPVEK
Sbjct:  234 RLVNFLAEEFKRKNKKDLTTNQRSLRRLRTAAERAKRTLSSSAQTSIEIDSLFEGIDFYTSITRARFEELCADLFRSTLEPVEK 317

HSP 2 Score: 119.398 bits (298), Expect = 6.535e-59
Identity = 60/109 (55.05%), Postives = 74/109 (67.89%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            D+K+DKS + ++VLVGGSTRIPKVQ+L+ DFFNGKE  +SINPDE           IL+G+ +   QD             ETAGG+MT LIPRN+TIPTKK +VFSTY
Sbjct:  321 DSKLDKSQIDEIVLVGGSTRIPKVQKLVSDFFNGKEPNRSINPDEAVAYGAAVQAAILTGDQSSTTQDLLLLDVAPLSLGIETAGGIMTKLIPRNSTIPTKKSEVFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP73_YEAST (Heat shock protein SSA3 OS=Saccharomyces cerevisiae GN=SSA3 PE=1 SV=3)

HSP 1 Score: 127.102 bits (318), Expect = 1.888e-58
Identity = 62/84 (73.81%), Postives = 72/84 (85.71%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VNH   EFKRK KKDIS N R+LRRLRTA ERAKR LSS++QT+IEIDSL+EG+DFYT++TRARFEEL  DLFR  +EPVEK
Sbjct:  234 RLVNHLATEFKRKTKKDISNNQRSLRRLRTAAERAKRALSSSSQTSIEIDSLFEGMDFYTSLTRARFEELCADLFRSTLEPVEK 317

HSP 2 Score: 120.168 bits (300), Expect = 1.888e-58
Identity = 59/110 (53.64%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +D+K+DKS + ++VLVGGSTRIPK+Q+L+ DFFNGKE  +SINPDE           IL+G+ + K QD             ETAGG+MT LIPRN+TIPTKK + FSTY
Sbjct:  320 KDSKLDKSQIDEIVLVGGSTRIPKIQKLVSDFFNGKEPNRSINPDEAVAYGAAVQAAILTGDQSTKTQDLLLLDVAPLSLGIETAGGIMTKLIPRNSTIPTKKSETFSTY 429

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP72_ANOAL (Heat shock protein 70 A2 OS=Anopheles albimanus GN=HSP70A2 PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.184e-58
Identity = 62/84 (73.81%), Postives = 72/84 (85.71%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            RMV HFV+EFKRK KKD+S N RALRRLRTACERAKRTLSS+ + TIEID+L +GID+YT I+RARFEEL  DLFR  ++PVEK
Sbjct:  235 RMVAHFVEEFKRKFKKDLSKNARALRRLRTACERAKRTLSSSTEATIEIDALMDGIDYYTKISRARFEELCSDLFRSTLQPVEK 318

HSP 2 Score: 120.553 bits (301), Expect = 4.184e-58
Identity = 63/109 (57.80%), Postives = 74/109 (67.89%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDKSS+HD+VLVGGSTRIPKVQ LLQ+FF GK L  SINPDE           ILSG+ ++K+QD             ETAGGVMT LI RN+ IP K+ ++FSTY
Sbjct:  322 DAKMDKSSIHDIVLVGGSTRIPKVQSLLQNFFAGKSLNLSINPDEAVAYGAAVQAAILSGDKDDKIQDVLLVDVAPLSLGIETAGGVMTKLIERNSRIPCKQTKIFSTY 430

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP71_ANOAL (Heat shock protein 70 A1 OS=Anopheles albimanus GN=HSP70A1 PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.184e-58
Identity = 62/84 (73.81%), Postives = 72/84 (85.71%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            RMV HFV+EFKRK KKD+S N RALRRLRTACERAKRTLSS+ + TIEID+L +GID+YT I+RARFEEL  DLFR  ++PVEK
Sbjct:  235 RMVAHFVEEFKRKFKKDLSKNARALRRLRTACERAKRTLSSSTEATIEIDALMDGIDYYTKISRARFEELCSDLFRSTLQPVEK 318

HSP 2 Score: 120.553 bits (301), Expect = 4.184e-58
Identity = 63/109 (57.80%), Postives = 74/109 (67.89%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAKMDKSS+HD+VLVGGSTRIPKVQ LLQ+FF GK L  SINPDE           ILSG+ ++K+QD             ETAGGVMT LI RN+ IP K+ ++FSTY
Sbjct:  322 DAKMDKSSIHDIVLVGGSTRIPKVQSLLQNFFAGKSLNLSINPDEAVAYGAAVQAAILSGDKDDKIQDVLLVDVAPLSLGIETAGGVMTKLIERNSRIPCKQTKIFSTY 430

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP74_TRYBB (Heat shock 70 kDa protein 4 OS=Trypanosoma brucei brucei PE=2 SV=2)

HSP 1 Score: 124.02 bits (310), Expect = 5.454e-58
Identity = 67/113 (59.29%), Postives = 76/113 (67.26%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  +DAKMDK +VHDVVLVGGSTRIPKV QL+ DFF GKEL KSINPDE  X        IL+G  +++ +              ETAGGVMT LI RNTTIPTKK Q+FSTY
Sbjct:  322 RVLQDAKMDKRAVHDVVLVGGSTRIPKVMQLVSDFFGGKELNKSINPDEAVXYGAAVQAFILTGGKSKQTEGLLLLDVAPLTLGIETAGGVMTALIKRNTTIPTKKSQIFSTY 434

HSP 2 Score: 121.709 bits (304), Expect = 5.454e-58
Identity = 63/85 (74.12%), Postives = 71/85 (83.53%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V HF +EFKRKNK KD+S N RALRRLRTACERAKRTLSS AQ TIEID+L+E IDF  TITRARFEEL  DLFR  ++PVE+
Sbjct:  238 RLVAHFTEEFKRKNKGKDLSSNLRALRRLRTACERAKRTLSSAAQATIEIDALFENIDFQATITRARFEELCGDLFRGTLQPVER 322

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_AJECA (Heat shock 70 kDa protein OS=Ajellomyces capsulata GN=HSP70 PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.008e-56
Identity = 66/88 (75.00%), Postives = 71/88 (80.68%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKK-DISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEKCER 263
            R+VNHFV EFKRK KK   +   RALRR  TACERAKRTLSS AQT+IEIDSLYEGIDFYT+ITRARFEEL  DLFR  MEPVE+  R
Sbjct:  234 RLVNHFVSEFKRKFKKISPAERARALRRSPTACERAKRTLSSAAQTSIEIDSLYEGIDFYTSITRARFEELCQDLFRSTMEPVERVLR 321

HSP 2 Score: 118.627 bits (296), Expect = 1.008e-56
Identity = 64/114 (56.14%), Postives = 76/114 (66.67%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEK-VQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  RDAK+DKSSVH++VLVGGSTRIP++Q+L+ DFFNGKE  KSINPDE           ILSG+ + K   +             ETAGGVMT LI RNTTIPTKK + FST+
Sbjct:  318 RVLRDAKIDKSSVHEIVLVGGSTRIPRIQKLVSDFFNGKEPNKSINPDEAVAYGAAVQAAILSGDTSSKSTNEILLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSETFSTF 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP74_PARLI (Heat shock 70 kDa protein IV OS=Paracentrotus lividus GN=HSP70IV PE=3 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 1.315e-56
Identity = 62/84 (73.81%), Postives = 73/84 (86.90%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VNH   EFKRK KKD+  NPRA+RRLRTA ERAKRTLSS+AQ  IE+DSL+EGIDFYT+I+RARFE+L  DLFRKC+EPVE+
Sbjct:  236 RLVNHTSLEFKRKYKKDMRTNPRAIRRLRTAAERAKRTLSSSAQANIEVDSLFEGIDFYTSISRARFEDLCSDLFRKCLEPVER 319

HSP 2 Score: 112.849 bits (281), Expect = 1.315e-56
Identity = 57/109 (52.29%), Postives = 72/109 (66.06%), Query Frame = 1

Query:  262 DAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            DAK+DK  +  VVLVGGSTRIPK+Q+LLQ+F NGKEL KSINPDE           ILSG+ +++++D             ETAGGVM+ +I RNT +PTK  Q F+TY
Sbjct:  323 DAKIDKKKIDTVVLVGGSTRIPKIQKLLQEFLNGKELNKSINPDEAVAYGAAVQAAILSGDKSDEIKDVLLVDVAPLSLGIETAGGVMSKIIERNTRVPTKASQTFTTY 431

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_PLACB (Heat shock 70 kDa protein OS=Plasmodium cynomolgi (strain Berok) PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.714e-56
Identity = 64/110 (58.18%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +DA MDK SVH+VVLVGGSTRIPK+Q L+++FFNGKE C+SINPDE           ILSG+ +  VQD             ETAGGVMT LI RNTTIP KK Q+F+TY
Sbjct:  335 KDAMMDKKSVHEVVLVGGSTRIPKIQTLIKEFFNGKEACRSINPDEAVAYGAAVQAAILSGDQSNAVQDLLLLDVCSLSLGLETAGGVMTKLIERNTTIPAKKSQIFTTY 444

HSP 2 Score: 113.62 bits (283), Expect = 1.714e-56
Identity = 58/85 (68.24%), Postives = 70/85 (82.35%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VN  V++FKRKN+ KD+S N RALRRLRT CERAKRTLSS+ Q TIEIDSL+EGID+  T++RARFEEL +D FR  + PVEK
Sbjct:  248 RLVNFCVEDFKRKNRGKDLSKNSRALRRLRTQCERAKRTLSSSTQATIEIDSLFEGIDYSVTVSRARFEELCIDYFRDTLIPVEK 332

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_PLAFA (Heat shock 70 kDa protein OS=Plasmodium falciparum PE=2 SV=2)

HSP 1 Score: 127.102 bits (318), Expect = 1.714e-56
Identity = 64/110 (58.18%), Postives = 75/110 (68.18%), Query Frame = 1

Query:  259 RDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            +DA MDK SVH+VVLVGGSTRIPK+Q L+++FFNGKE C+SINPDE           ILSG+ +  VQD             ETAGGVMT LI RNTTIP KK Q+F+TY
Sbjct:  335 KDAMMDKKSVHEVVLVGGSTRIPKIQTLIKEFFNGKEACRSINPDEAVAYGAAVQAAILSGDQSNAVQDLLLLDVCSLSLGLETAGGVMTKLIERNTTIPAKKSQIFTTY 444

HSP 2 Score: 113.62 bits (283), Expect = 1.714e-56
Identity = 58/85 (68.24%), Postives = 70/85 (82.35%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+VN  V++FKRKN+ KD+S N RALRRLRT CERAKRTLSS+ Q TIEIDSL+EGID+  T++RARFEEL +D FR  + PVEK
Sbjct:  248 RLVNFCVEDFKRKNRGKDLSKNSRALRRLRTQCERAKRTLSSSTQATIEIDSLFEGIDYSVTVSRARFEELCIDYFRDTLIPVEK 332

[Close]

BLAST of CB417297 vs. ExPASy Swiss-Prot
Match: HSP70_LEIMA (Heat shock 70 kDa protein (Fragment) OS=Leishmania major GN=HSP70 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 8.434e-56
Identity = 67/113 (59.29%), Postives = 75/113 (66.37%), Query Frame = 1

Query:  250 RNARDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEXXXXXXXXXXXILSGEGNEKVQDXXXXXXXXXXXXXETAGGVMTVLIPRNTTIPTKKEQVFSTY 588
            R  +DAKMDK SVHDVVLVGGSTRIPKVQ L+ DFF GKEL KSINPDE           IL+G  +++ +              ETAGGVMT LI RNTTIPTKK Q+FSTY
Sbjct:  322 RVLQDAKMDKRSVHDVVLVGGSTRIPKVQSLVSDFFGGKELNKSINPDEAVAYGAAVQAFILTGGKSKQTEGLLLLDVTPLTLGIETAGGVMTALIKRNTTIPTKKSQIFSTY 434

HSP 2 Score: 113.235 bits (282), Expect = 8.434e-56
Identity = 58/85 (68.24%), Postives = 69/85 (81.18%), Query Frame = 3

Query:    3 RMVNHFVQEFKRKNK-KDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYTTITRARFEELNMDLFRKCMEPVEK 254
            R+V  F +EFKRKNK K+++ + RALRRLRTACERAKRTLSS  Q TIEID+L+E IDF  TITRARFEEL  DLFR  ++PVE+
Sbjct:  238 RLVTFFTEEFKRKNKGKNLASSHRALRRLRTACERAKRTLSSATQATIEIDALFENIDFQATITRARFEELCGDLFRSTIQPVER 322

The following BLAST results are available for this feature:

BLAST of CB417297 vs. ExPASy Swiss-Prot

BLAST of CB417297 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
HSP74_YEAST	6.535e-59	76.19	Heat shock protein SSA4 OS=Saccharomyces cerevisia...	[more]
HSP73_YEAST	1.888e-58	73.81	Heat shock protein SSA3 OS=Saccharomyces cerevisia...	[more]
HSP72_ANOAL	4.184e-58	73.81	Heat shock protein 70 A2 OS=Anopheles albimanus GN...	[more]
HSP71_ANOAL	4.184e-58	73.81	Heat shock protein 70 A1 OS=Anopheles albimanus GN...	[more]
HSP74_TRYBB	5.454e-58	59.29	Heat shock 70 kDa protein 4 OS=Trypanosoma brucei ...	[more]
HSP70_AJECA	1.008e-56	75.00	Heat shock 70 kDa protein OS=Ajellomyces capsulata...	[more]
HSP74_PARLI	1.315e-56	73.81	Heat shock 70 kDa protein IV OS=Paracentrotus livi...	[more]
HSP70_PLACB	1.714e-56	58.18	Heat shock 70 kDa protein OS=Plasmodium cynomolgi ...	[more]
HSP70_PLAFA	1.714e-56	58.18	Heat shock 70 kDa protein OS=Plasmodium falciparum...	[more]
HSP70_LEIMA	8.434e-56	59.29	Heat shock 70 kDa protein (Fragment) OS=Leishmania...	[more]

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Properties


Property Name	Value
Genbank description	EST0370 Mature peel (flavedo) cDNA subtraction library Citrus sinensis cDNA clone 6P015 similar to 70 kDa heat shock protein (acc# AAL24367), mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CB417297 ID=CB417297; Name=CB417297; organism=Citrus sinensis; type=EST; length=590bp
ACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGGAAAAACAAGAAG
GATATTAGTGGAAACCCCAGAGCGTTGAGGAGGTTGAGAACTGCCTGTGA
AAGGGCTAAGAGGACCCTGTCATCAACCGCACAGACTACAATTGAGATTG
ATTCTTTATATGAGGGTATTGATTTCTACACAACCATTACAAGGGCTAGA
TTCGAAGAGTTGAACATGGATTTGTTCAGGAAGTGTATGGAGCCAGTGGA
GAAATGCGAGAGATGCTAAGATGGACAAGAGCAGTGTCCATGATGTGGTT
CTTGTTGGTGGGTCTACTAGGATCCCAAAAGTCCAGCAACTGTTGCAGGA
CTTCTTCAATGGAAAAGAGCTATGCAAGAGCATCAACCCTGATGAAGCGG
TTGCCTACGGTGCTGCTGTGCAGGCTGCAATCTTGAGTGGTGAGGGTAAT
GAGAAGGTTCAGGACCTCTTATTGCTTGACGTCACCCCTCTATCGCTGGG
TTTGGAGACTGCTGGTGGAGTCATGACTGTGTTGATTCCAAGGAACACCA
CTATTCCAACCAAGAAGGAGCAGGTGTTCTCCACATACTC