CF417199 | Citrus Genome Database

Overview


Name	CF417199
Unique Name	CF417199
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	697

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC2A_ANTMA (Cell division control protein 2 homolog A OS=Antirrhinum majus GN=CDC2A PE=2 SV=2)

HSP 1 Score: 177.178 bits (448), Expect = 1.950e-54
Identity = 93/198 (46.97%), Postives = 123/198 (62.12%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME+YEK+EK+GEGTYG VYKA+D+ T + +ALKK RLE ++EG+P TA+RE+SLL+ +     +VRL  V H                        +  LYLVFEYLD DLKK +DS  +        P L++ FLYQ+ +G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MEQYEKVEKIGEGTYGVVYKARDRVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHG-NIVRLQDVVH-----------------------SEKRLYLVFEYLDLDLKKHMDSCPEFSQD----PRLVKMFLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNALKLADFGLARAFGIPVRTFTHEVVTLWYR 170

HSP 2 Score: 56.225 bits (134), Expect = 1.950e-54
Identity = 24/31 (77.42%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS HYST VD+WSVGCIFAEMV ++
Sbjct:  171 APEILLGSRHYSTPVDVWSVGCIFAEMVNQR 201

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC22_MEDSA (Cell division control protein 2 homolog 2 OS=Medicago sativa GN=CDC2B PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 3.315e-54
Identity = 95/198 (47.98%), Postives = 124/198 (62.63%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME+YEK+EK+GEGTYG VYKA+D+AT + +ALKK RLE ++EG+P TA+RE+SLL+ + Q   +VRL  V H                        +  LYLVFEYLD DLKKF+DS  +    +      I+ FLYQ+  G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MEQYEKVEKIGEGTYGVVYKARDRATNETIALKKIRLEQEDEGVPSTAIREISLLKEM-QHRNIVRLQDVVH-----------------------SEKRLYLVFEYLDLDLKKFMDSSPEFAKDQ----RQIKMFLYQILCGIAYCHSHRVLHRDLKPQNLLIDRSSNAVKLADFGLARAFGIPVRTFTHEVVTLWYR 170

HSP 2 Score: 55.8398 bits (133), Expect = 3.315e-54
Identity = 23/31 (74.19%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS HYST VD+WSVGCIFAEM+ ++
Sbjct:  171 APEILLGSRHYSTPVDVWSVGCIFAEMINQR 201

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC2_CHERU (Cell division control protein 2 homolog OS=Chenopodium rubrum GN=CDC2 PE=2 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 4.321e-54
Identity = 93/198 (46.97%), Postives = 122/198 (61.62%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            M++YEK+EK+GEGTYG VYKA+DK T + +ALKK RLE ++EG+P TA+RE+SLL+ +     +VRL  V H                        +  LYLVFEYLD DLKK +DS           P +I+ FLYQ+ +G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHG-NIVRLQDVVH-----------------------SEKRLYLVFEYLDLDLKKHMDSCPDFAKD----PRMIKRFLYQILRGIAYCHSHRVLHRDLKPQNLLIDRQTNALKLADFGLARAFGIPVRTFTHEVVTLWYR 170

HSP 2 Score: 56.225 bits (134), Expect = 4.321e-54
Identity = 24/31 (77.42%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS HYST VD+WSVGCIFAEMV ++
Sbjct:  171 APEILLGSRHYSTPVDVWSVGCIFAEMVNQK 201

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC2_VIGAC (Cell division control protein 2 homolog OS=Vigna aconitifolia GN=CDC2 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 5.633e-54
Identity = 93/198 (46.97%), Postives = 122/198 (61.62%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME+YEK+EK+GEGTYG VYKA+D+ T + +ALKK RLE ++EG+P TA+RE+SLL+ + Q   +VRL  V H                        +  LYLVFEYLD DLKK +DS  +        P  ++ FLYQ+  G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MEQYEKVEKIGEGTYGVVYKARDRVTNETIALKKIRLEQEDEGVPSTAIREISLLKEM-QHRNIVRLQDVVH-----------------------SEKRLYLVFEYLDLDLKKHMDSSPEFVK----DPRQVKMFLYQILCGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYR 170

HSP 2 Score: 57.7658 bits (138), Expect = 5.633e-54
Identity = 25/31 (80.65%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS HYST VD+WSVGCIFAEMV R+
Sbjct:  171 APEILLGSRHYSTPVDVWSVGCIFAEMVNRR 201

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDKA2_ORYSJ (Cyclin-dependent kinase A-2 OS=Oryza sativa subsp. japonica GN=CDKA-2 PE=2 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 7.344e-54
Identity = 93/198 (46.97%), Postives = 123/198 (62.12%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME+YEK+EK+GEGTYG VYK K + T + +ALKK RLE ++EG+P TA+RE+SLL+ + Q   +VRL  V H +  +                       YLVFEYLD DLKK +DS     N R     +++SFLYQ+ +G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MEQYEKVEKIGEGTYGVVYKGKHRHTNETIALKKIRLEQEDEGVPSTAIREISLLKEM-QHRNIVRLQDVVHKEKCI-----------------------YLVFEYLDLDLKKHMDSSPDFKNHR-----IVKSFLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYR 169

HSP 2 Score: 56.6102 bits (135), Expect = 7.344e-54
Identity = 24/31 (77.42%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLG+ HYST VDMWSVGCIFAEMV ++
Sbjct:  170 APEILLGARHYSTPVDMWSVGCIFAEMVNQK 200

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC2_VIGUN (Cell division control protein 2 homolog OS=Vigna unguiculata GN=CDC2 PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 1.248e-53
Identity = 93/198 (46.97%), Postives = 122/198 (61.62%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME+YEK+EK+GEGTYG VYKA+D+ T + +ALKK RLE ++EG+P TA+RE+SLL+ + Q   +VRL  V H                        +  LYLVFEYLD DLKK +DS  +        P  ++ FLYQ+  G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MEQYEKVEKIGEGTYGVVYKARDRVTDETIALKKIRLEQEDEGVPSTAIREISLLKEM-QHRNIVRLQDVVH-----------------------SEKRLYLVFEYLDLDLKKHMDSSPEFVK----DPRQVKMFLYQILCGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYR 170

HSP 2 Score: 56.9954 bits (136), Expect = 1.248e-53
Identity = 24/31 (77.42%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS HYST VD+WSVGC+FAEMV R+
Sbjct:  171 APEILLGSRHYSTPVDVWSVGCLFAEMVNRR 201

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDKA1_ARATH (Cyclin-dependent kinase A-1 OS=Arabidopsis thaliana GN=CDKA-1 PE=1 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 7.983e-53
Identity = 91/198 (45.96%), Postives = 123/198 (62.12%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            M++YEK+EK+GEGTYG VYKA+DK T + +ALKK RLE ++EG+P TA+RE+SLL+ +  S  +V+L  V H                        +  LYLVFEYLD DLKK +DS             +I+++LYQ+ +G+A+CHSH VLHRDLKPQN               GL RAF +P++++THE+VTLWYR
Sbjct:    1 MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHS-NIVKLQDVVH-----------------------SEKRLYLVFEYLDLDLKKHMDSTPDFSKDL----HMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYR 170

HSP 2 Score: 55.4546 bits (132), Expect = 7.983e-53
Identity = 23/31 (74.19%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS HYST VD+WSVGCIFAEM+ ++
Sbjct:  171 APEILLGSHHYSTPVDIWSVGCIFAEMISQK 201

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDK3_HUMAN (Cell division protein kinase 3 OS=Homo sapiens GN=CDK3 PE=1 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 2.304e-52
Identity = 97/198 (48.99%), Postives = 125/198 (63.13%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            M+ ++K+EK+GEGTYG VYKAK++ TGQLVALKK RL+++ EG+P TA+RE+SLL+ L     +VRLL V H                        +  LYLVFE+L  DLKK++DS         LP  LI+S+L+QL +GV+ CHSH V+HRDLKPQN               GL RAF VPL++YTHE+VTLWYR
Sbjct:    1 MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHP-NIVRLLDVVH-----------------------NERKLYLVFEFLSQDLKKYMDS----TPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQN-LLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYR 169

HSP 2 Score: 54.299 bits (129), Expect = 2.304e-52
Identity = 23/31 (74.19%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLGS  Y+TAVD+WS+GCIFAEMV R+
Sbjct:  170 APEILLGSKFYTTAVDIWSIGCIFAEMVTRK 200

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDK2_RAT (Cell division protein kinase 2 OS=Rattus norvegicus GN=Cdk2 PE=2 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.916e-52
Identity = 96/198 (48.48%), Postives = 126/198 (63.64%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME ++K+EK+GEGTYG VYKAK+K TG++VALKK RL+ + EG+P TA+RE+SLL+ L+    +V+LL V H +                       + LYLVFE+L  DLKKF+D+      P P    LI+S+L+QL +G+A CHSH VLHRDLKPQN               GL RAF VP+++YTHE+VTLWYR
Sbjct:    1 MENFQKVEKIGEGTYGVVYKAKNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHP-NIVKLLDVIHTE-----------------------NKLYLVFEFLHQDLKKFMDASALTGLPLP----LIKSYLFQLLQGLAFCHSHRVLHRDLKPQN-LLINAEGSIKLADFGLARAFGVPVRTYTHEVVTLWYR 169

HSP 2 Score: 53.9138 bits (128), Expect = 3.916e-52
Identity = 23/31 (74.19%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLG  +YSTAVD+WS+GCIFAEMV R+
Sbjct:  170 APEILLGCKYYSTAVDIWSLGCIFAEMVTRR 200

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDK2_CRIGR (Cell division protein kinase 2 OS=Cricetulus griseus GN=CDK2 PE=2 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.916e-52
Identity = 96/198 (48.48%), Postives = 126/198 (63.64%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ME ++K+EK+GEGTYG VYKAK+K TG++VALKK RL+ + EG+P TA+RE+SLL+ L+    +V+LL V H +                       + LYLVFE+L  DLKKF+D+      P P    LI+S+L+QL +G+A CHSH VLHRDLKPQN               GL RAF VP+++YTHE+VTLWYR
Sbjct:    1 MENFQKVEKIGEGTYGVVYKAKNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHP-NIVKLLDVIHTE-----------------------NKLYLVFEFLHQDLKKFMDASAVTGIPLP----LIKSYLFQLLQGLAFCHSHRVLHRDLKPQN-LLINAEGSIKLADFGLARAFGVPVRTYTHEVVTLWYR 169

HSP 2 Score: 53.9138 bits (128), Expect = 3.916e-52
Identity = 23/31 (74.19%), Postives = 28/31 (90.32%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+LLG  +YSTAVD+WS+GCIFAEMV R+
Sbjct:  170 APEILLGCKYYSTAVDIWSLGCIFAEMVTRR 200

The following BLAST results are available for this feature:

BLAST of CF417199 vs. ExPASy Swiss-Prot

BLAST of CF417199 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 480


Match Name	E-value	Identity	Description
CDC2A_ANTMA	1.950e-54	46.97	Cell division control protein 2 homolog A OS=Antir...	[more]
CDC22_MEDSA	3.315e-54	47.98	Cell division control protein 2 homolog 2 OS=Medic...	[more]
CDC2_CHERU	4.321e-54	46.97	Cell division control protein 2 homolog OS=Chenopo...	[more]
CDC2_VIGAC	5.633e-54	46.97	Cell division control protein 2 homolog OS=Vigna a...	[more]
CDKA2_ORYSJ	7.344e-54	46.97	Cyclin-dependent kinase A-2 OS=Oryza sativa subsp....	[more]
CDC2_VIGUN	1.248e-53	46.97	Cell division control protein 2 homolog OS=Vigna u...	[more]
CDKA1_ARATH	7.983e-53	45.96	Cyclin-dependent kinase A-1 OS=Arabidopsis thalian...	[more]
CDK3_HUMAN	2.304e-52	48.99	Cell division protein kinase 3 OS=Homo sapiens GN=...	[more]
CDK2_RAT	3.916e-52	48.48	Cell division protein kinase 2 OS=Rattus norvegicu...	[more]
CDK2_CRIGR	3.916e-52	48.48	Cell division protein kinase 2 OS=Cricetulus grise...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-129 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-002_E10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417199 ID=CF417199; Name=CF417199; organism=Citrus sinensis; type=EST; length=697bp
AGAGGATGGAGAAGTACGAGAAGCTAGAGAAGGTGGGAGAAGGAACGTAC
GGGAAAGTGTACAAGGCAAAGGACAAAGCGACCGGGCAGTTGGTTGCTCT
GAAGAAAACAAGACTCGAAATGGATGAAGAAGGCATCCCCCCGACCGCTC
TCCGCGAAGTGTCCCTCCTCCAGATGCTCTCTCAATCCCTTTACGTCGTC
CGCCTCCTCTGCGTCGAACACGTGGACTCCTCTCTCAAATCCAACAACCC
CAGCCCCGACTCCGCTTCCGCCTTCGCCACCTCCAACAACGGCAAGTCTA
ATCTCTATCTCGTGTTTGAATACCTCGACACCGATCTAAAGAAATTCATC
GACTCGCACCGCAAGGGCCCCAATCCGAGGCCGCTGCCCCCCACTCTCAT
TCAGAGCTTCTTGTACCAGCTTTGCAAAGGCGTCGCCCACTGCCACAGCC
ACGGTGTCCTTCATCGCGACCTGAAGCCGCAGAATCTTCTCCTTGATAAG
GACAAGGGGATTCTCAAGATCGCCGATCTTGGCCTCGGACGCGCCTTCAC
CGTTCCTCTCAAAAGCTATACCCACGAGATTGTTACTCTCTGGTATAGGG
GCTCCCGAGGTGCTTCTTGGTTCCACTCATTACTCCACTGCTGTCGACAT
GTGGTCCGTTGGTTGCATTTTCGCTGAGATGGTTAAAAGGCAGGTTT