CF417199 | Citrus Genome Database

Overview


Name	CF417199
Unique Name	CF417199
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	697

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CC2H1_TRYBB (Cell division control protein 2 homolog 1 OS=Trypanosoma brucei brucei GN=CRK1 PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.551e-41
Identity = 83/196 (42.35%), Postives = 111/196 (56.63%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            +YE+L+K+GEG+YG V++A+D  TG +VA+K+ RLE +EEG+P TA+RE+S+L+ L     +VRLL V H                        +  L LVFE ++ DLKK++D H  G     L    IQ F+  L  GV  CH   VLHRDLKP N               GLGRAF +P+K +T E+VTLWYR
Sbjct:    4 RYERLQKIGEGSYGVVFRARDVTTGTIVAVKRIRLEKEEEGVPCTAIREISILKELRHE-NIVRLLDVCH-----------------------SEKRLTLVFECMEMDLKKYMD-HVGG----DLDAGTIQEFMRSLLSGVRFCHERNVLHRDLKPPN-LLISREKELKLADFGLGRAFGIPVKKFTQEVVTLWYR 169

HSP 2 Score: 49.6766 bits (117), Expect = 2.551e-41
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEM 681
            +P+VLLGST Y T VD+WSVGCIFAEM
Sbjct:  170 SPDVLLGSTQYGTPVDIWSVGCIFAEM 196

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PHO85_CANGA (Negative regulator of the PHO system OS=Candida glabrata GN=PHO85 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 5.641e-41
Identity = 81/196 (41.33%), Postives = 114/196 (58.16%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++++LEK+G GTY  VYK  +K+TG  VALK+ +L+  EEG P TA+RE+SL++ L     +VRL  V H ++ L                        LVFEY+D DLKK++DS   G  PR L   L++ F +QL +G+A CH + +LHRDLKPQN               GL RAF +P+ +++ E+VTLWYR
Sbjct:    5 QFKQLEKLGNGTYATVYKGLNKSTGVYVALKEVKLD-SEEGTPSTAIREISLMKELKHD-NIVRLYDVIHTENKLT-----------------------LVFEYMDNDLKKYMDSRTVGNAPRGLEMNLVKYFQWQLLEGLAFCHENKILHRDLKPQN-LLITKRGQLKLGDFGLARAFGIPVNTFSSEVVTLWYR 174

HSP 2 Score: 45.8246 bits (107), Expect = 5.641e-41
Identity = 18/28 (64.29%), Postives = 24/28 (85.71%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            AP+VL+GS  YST++D+WS GCI AEM+
Sbjct:  175 APDVLMGSRTYSTSIDIWSCGCILAEMI 202

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC2_CAEBR (Cell division control protein 2 homolog OS=Caenorhabditis briggsae GN=cdk-1 PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 9.568e-41
Identity = 76/198 (38.38%), Postives = 111/198 (56.06%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++ + KLEK+GEGTYG VYK +++ T  +VA+KK RLE ++EG+P TA+RE+SLL+ L                      +P+     A     N    LYL+FE+L  DLK+++D+  K      LP   ++S+ +Q+ + +  CH   V+HRDLKPQN               GL RA  +P++ YTHE+VTLWYR
Sbjct:   13 LDDFTKLEKIGEGTYGVVYKGRNRRTQAMVAMKKIRLESEDEGVPSTAVREISLLKEL---------------------QHPNVVGLEAVIMQEN---RLYLIFEFLSYDLKRYMDTLSK---EEYLPSETLKSYTFQILQAMCFCHQRRVIHRDLKPQN-LLVDEKGAIKLADFGLARAIGIPIRVYTHEVVTLWYR 182

HSP 2 Score: 48.9062 bits (115), Expect = 9.568e-41
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+L+G+  YS  VDMWS+GCIFAEM  ++
Sbjct:  183 APEILMGAQRYSMGVDMWSIGCIFAEMATKK 213

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PHO85_KLULA (Negative regulator of the PHO system OS=Kluyveromyces lactis GN=PHO85 PE=3 SV=2)

HSP 1 Score: 142.51 bits (358), Expect = 9.578e-41
Identity = 81/196 (41.33%), Postives = 114/196 (58.16%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++++LEKVG GTY  VYK  +K TG  VALK+ +L+  EEG P TA+RE+SL++ L     +VRL  V H ++ L                        LVFE++D DLKKF+D+  KG + + L   L++ F +QL +GVA CH + +LHRDLKPQN               GL RAF +P+ +++ E+VTLWYR
Sbjct:    6 QFKQLEKVGNGTYATVYKGLNKTTGVYVALKEVKLD-SEEGTPSTAIREISLMKELKHD-NIVRLFDVIHTENKLT-----------------------LVFEFMDNDLKKFMDNRNKGNSHKGLEMDLVKYFQWQLLQGVAFCHENRILHRDLKPQN-LLINNRGQLKLGDFGLARAFGIPVNTFSSEVVTLWYR 175

HSP 2 Score: 45.0542 bits (105), Expect = 9.578e-41
Identity = 17/28 (60.71%), Postives = 24/28 (85.71%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            AP+VL+GS +Y T++D+WS GCI AEM+
Sbjct:  176 APDVLMGSRNYCTSIDIWSCGCILAEMI 203

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PHO85_YEAST (Cyclin-dependent protein kinase PHO85 OS=Saccharomyces cerevisiae GN=PHO85 PE=1 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 1.626e-40
Identity = 80/196 (40.82%), Postives = 113/196 (57.65%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++++LEK+G GTY  VYK  +K TG  VALK+ +L+  EEG P TA+RE+SL++ L     +VRL  V H ++ L                        LVFE++D DLKK++DS   G  PR L   L++ F +QL +G+A CH + +LHRDLKPQN               GL RAF +P+ +++ E+VTLWYR
Sbjct:    6 QFKQLEKLGNGTYATVYKGLNKTTGVYVALKEVKLD-SEEGTPSTAIREISLMKELKHE-NIVRLYDVIHTENKLT-----------------------LVFEFMDNDLKKYMDSRTVGNTPRGLELNLVKYFQWQLLQGLAFCHENKILHRDLKPQN-LLINKRGQLKLGDFGLARAFGIPVNTFSSEVVTLWYR 175

HSP 2 Score: 45.8246 bits (107), Expect = 1.626e-40
Identity = 18/28 (64.29%), Postives = 24/28 (85.71%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            AP+VL+GS  YST++D+WS GCI AEM+
Sbjct:  176 APDVLMGSRTYSTSIDIWSCGCILAEMI 203

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: CDC2_CAEEL (Cell division control protein 2 homolog OS=Caenorhabditis elegans GN=cdk-1 PE=1 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 3.592e-40
Identity = 76/198 (38.38%), Postives = 109/198 (55.05%), Query Frame = 3

Query:    6 MEKYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            +  + KLEK+GEGTYG VYK K++ T  +VA+KK RLE ++EG+P TA+RE+SLL+ L                      +P+     A     N    L+L+FE+L  DLK+++D   K      LP   ++S+ +Q+ + +  CH   V+HRDLKPQN               GL RA  +P++ YTHE+VTLWYR
Sbjct:   19 LNDFTKLEKIGEGTYGVVYKGKNRRTNAMVAMKKIRLESEDEGVPSTAVREISLLKEL---------------------QHPNVVGLEAVIMQEN---RLFLIFEFLSFDLKRYMDQLGK---DEYLPLETLKSYTFQILQAMCFCHQRRVIHRDLKPQN-LLVDNNGAIKLADFGLARAIGIPIRVYTHEVVTLWYR 188

HSP 2 Score: 48.9062 bits (115), Expect = 3.592e-40
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMVKRQ 693
            APE+L+G+  YS  VDMWS+GCIFAEM  ++
Sbjct:  189 APEILMGAQRYSMGVDMWSIGCIFAEMATKK 219

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PHO85_YARLI (Negative regulator of the PHO system OS=Yarrowia lipolytica GN=PHO85 PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 7.958e-40
Identity = 80/196 (40.82%), Postives = 114/196 (58.16%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++++LEK+GEGTY  VYK +++ TGQLVALK+  L+  EEG P TA+RE+SL++ L     +V L  V H ++ L                        LVFEY+D DLKKF+D++    N   L    ++ F+YQL +G+  CH + VLHRDLKPQN               GL RAF +P+ ++++E+VTLWYR
Sbjct:    6 QFQQLEKLGEGTYATVYKGRNRTTGQLVALKEINLD-SEEGTPSTAIREISLMKELKHE-NIVTLYDVIHTENKLN-----------------------LVFEYMDKDLKKFMDTN---GNKGALETKQVKWFMYQLLRGILFCHDNRVLHRDLKPQN-LLINAKGQLKLADFGLARAFGIPVNTFSNEVVTLWYR 172

HSP 2 Score: 45.8246 bits (107), Expect = 7.958e-40
Identity = 19/27 (70.37%), Postives = 23/27 (85.19%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEM 681
            AP+VLLGS  YST++D+WS GCI AEM
Sbjct:  173 APDVLLGSRTYSTSIDIWSAGCIMAEM 199

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PHO85_CANAL (Negative regulator of the PHO system OS=Candida albicans GN=PHO85 PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.349e-39
Identity = 79/196 (40.31%), Postives = 114/196 (58.16%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++++LEK+GEGTY  VYK +++ATG LVALK+  L+  EEG P TA+RE+SL++ L     +V L  V H ++ L                        LVFEY+D DLKK+++ H +      L   +++SF++QL KG+  CH + VLHRDLKPQN               GL RAF +P  ++++E+VTLWYR
Sbjct:    7 QFQQLEKLGEGTYATVYKGRNRATGALVALKEISLD-SEEGTPSTAIREISLMKELDHE-NIVTLYDVIHTENKLT-----------------------LVFEYMDKDLKKYMEVHGQ---QSALDLKVVKSFMFQLLKGIMFCHDNRVLHRDLKPQN-LLINNKGELKLGDFGLARAFGIPFNTFSNEVVTLWYR 173

HSP 2 Score: 46.9802 bits (110), Expect = 1.349e-39
Identity = 19/27 (70.37%), Postives = 24/27 (88.89%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEM 681
            AP+VLLGS  Y+T++D+WS GCIFAEM
Sbjct:  174 APDVLLGSRAYTTSIDIWSAGCIFAEM 200

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PHO85_ASHGO (Negative regulator of the PHO system OS=Ashbya gossypii GN=PHO85 PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.759e-39
Identity = 80/196 (40.82%), Postives = 113/196 (57.65%), Query Frame = 3

Query:   12 KYEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            ++++LE++G GTY  VYK  +K TG  VALK+ +L+  EEG P TA+RE+SL++ L     +VRL  V H ++ L                        LVFE++D DLKKF+DS      PR L  +L++ F +QL +GVA CH + +LHRDLKPQN               GL RAF +P+ +++ E+VTLWYR
Sbjct:    8 RFKQLERLGNGTYATVYKGLNKTTGLYVALKEVKLD-SEEGTPSTAIREISLMKELKHE-NIVRLYDVIHTENKLT-----------------------LVFEFMDNDLKKFMDSRLDREMPRGLELSLVKYFQWQLLQGVAFCHENRILHRDLKPQN-LLINNKGQLKLGDFGLARAFGIPVNTFSSEVVTLWYR 177

HSP 2 Score: 43.8986 bits (102), Expect = 1.759e-39
Identity = 17/28 (60.71%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEMV 684
            AP+VL+GS  Y T++D+WS GCI AEM+
Sbjct:  178 APDVLMGSRTYCTSIDIWSCGCILAEMI 205

[Close]

BLAST of CF417199 vs. ExPASy Swiss-Prot
Match: PEF1_SCHPO (Serine/threonine-protein kinase pef1 OS=Schizosaccharomyces pombe GN=pef1 PE=2 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.761e-39
Identity = 77/195 (39.49%), Postives = 115/195 (58.97%), Query Frame = 3

Query:   15 YEKLEKVGEGTYGKVYKAKDKATGQLVALKKTRLEMDEEGIPPTALREVSLLQMLSQSLYVVRLLCVEHVDSSLKSNNPSPDSASAFATSNNGKSNLYLVFEYLDTDLKKFIDSHRKGPNPRPLPPTLIQSFLYQLCKGVAHCHSHGVLHRDLKPQNXXXXXXXXXXXXXXXGLGRAFTVPLKSYTHEIVTLWYR 599
            Y++LEK+GEGTY  VYK +++ TG++VALK  R++ D EG P TA+RE+SL++ L                      +P+  S S    + N    L LVFEY++ DLKK++D++    N   LPP+ +++F  QL KG++ CH + VLHRDLKPQN               GL R+  +P+ ++++E+VTLWYR
Sbjct:    3 YQRLEKLGEGTYAHVYKGQNRVTGEIVALKVIRIDAD-EGTPSTAIREISLMKEL---------------------RHPNIMSLSDVLQTEN---KLMLVFEYMEKDLKKYMDTY---GNQGALPPSQVKNFTQQLLKGISFCHENRVLHRDLKPQN-LLINSRGELKLADFGLARSIGIPVNTFSNEVVTLWYR 168

HSP 2 Score: 46.9802 bits (110), Expect = 1.761e-39
Identity = 20/27 (74.07%), Postives = 24/27 (88.89%), Query Frame = 1

Query:  601 APEVLLGSTHYSTAVDMWSVGCIFAEM 681
            AP+VLLGS  YST++D+WSVGCI AEM
Sbjct:  169 APDVLLGSRVYSTSIDIWSVGCIMAEM 195

The following BLAST results are available for this feature:

BLAST of CF417199 vs. ExPASy Swiss-Prot

BLAST of CF417199 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 480


Match Name	E-value	Identity	Description
CC2H1_TRYBB	2.551e-41	42.35	Cell division control protein 2 homolog 1 OS=Trypa...	[more]
PHO85_CANGA	5.641e-41	41.33	Negative regulator of the PHO system OS=Candida gl...	[more]
CDC2_CAEBR	9.568e-41	38.38	Cell division control protein 2 homolog OS=Caenorh...	[more]
PHO85_KLULA	9.578e-41	41.33	Negative regulator of the PHO system OS=Kluyveromy...	[more]
PHO85_YEAST	1.626e-40	40.82	Cyclin-dependent protein kinase PHO85 OS=Saccharom...	[more]
CDC2_CAEEL	3.592e-40	38.38	Cell division control protein 2 homolog OS=Caenorh...	[more]
PHO85_YARLI	7.958e-40	40.82	Negative regulator of the PHO system OS=Yarrowia l...	[more]
PHO85_CANAL	1.349e-39	40.31	Negative regulator of the PHO system OS=Candida al...	[more]
PHO85_ASHGO	1.759e-39	40.82	Negative regulator of the PHO system OS=Ashbya gos...	[more]
PEF1_SCHPO	1.761e-39	39.49	Serine/threonine-protein kinase pef1 OS=Schizosacc...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_115000-129 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-002_E10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417199 ID=CF417199; Name=CF417199; organism=Citrus sinensis; type=EST; length=697bp
AGAGGATGGAGAAGTACGAGAAGCTAGAGAAGGTGGGAGAAGGAACGTAC
GGGAAAGTGTACAAGGCAAAGGACAAAGCGACCGGGCAGTTGGTTGCTCT
GAAGAAAACAAGACTCGAAATGGATGAAGAAGGCATCCCCCCGACCGCTC
TCCGCGAAGTGTCCCTCCTCCAGATGCTCTCTCAATCCCTTTACGTCGTC
CGCCTCCTCTGCGTCGAACACGTGGACTCCTCTCTCAAATCCAACAACCC
CAGCCCCGACTCCGCTTCCGCCTTCGCCACCTCCAACAACGGCAAGTCTA
ATCTCTATCTCGTGTTTGAATACCTCGACACCGATCTAAAGAAATTCATC
GACTCGCACCGCAAGGGCCCCAATCCGAGGCCGCTGCCCCCCACTCTCAT
TCAGAGCTTCTTGTACCAGCTTTGCAAAGGCGTCGCCCACTGCCACAGCC
ACGGTGTCCTTCATCGCGACCTGAAGCCGCAGAATCTTCTCCTTGATAAG
GACAAGGGGATTCTCAAGATCGCCGATCTTGGCCTCGGACGCGCCTTCAC
CGTTCCTCTCAAAAGCTATACCCACGAGATTGTTACTCTCTGGTATAGGG
GCTCCCGAGGTGCTTCTTGGTTCCACTCATTACTCCACTGCTGTCGACAT
GTGGTCCGTTGGTTGCATTTTCGCTGAGATGGTTAAAAGGCAGGTTT