CF504131 | Citrus Genome Database

Overview


Name	CF504131
Unique Name	CF504131
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	342

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF504131_ssr283	microsatellite	CF504131_ssr283:283..298.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947	contig	Csv1_Contig10947:167..505.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_BRAJU (S-adenosylmethionine synthetase 2 OS=Brassica juncea GN=MSAMS2 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.121e-22
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            GRFLKTAAYGHFGRDD DFTWE+VKPLK  K+ A
Sbjct:  360 GRFLKTAAYGHFGRDDADFTWEVVKPLKSNKVQA 393

HSP 2 Score: 61.6178 bits (148), Expect = 2.121e-22
Identity = 29/35 (82.86%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL+IVKE+FDFRPGMISINLDLKRGGN + L+
Sbjct:  330 DKEILEIVKESFDFRPGMISINLDLKRGGNGRFLK 364

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_POPTR (S-adenosylmethionine synthetase 2 OS=Populus trichocarpa GN=METK2 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.759e-22
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDDPDFTWE+VKPLK EK
Sbjct:  360 GRFLKTAAYGHFGRDDPDFTWEVVKPLKSEK 390

HSP 2 Score: 57.7658 bits (138), Expect = 2.759e-22
Identity = 27/35 (77.14%), Postives = 31/35 (88.57%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL IVKE FDFRPGMI+I+LDLKRGGN + L+
Sbjct:  330 DKEILNIVKEKFDFRPGMIAISLDLKRGGNGRFLK 364

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_SOLBR (S-adenosylmethionine synthetase OS=Solanum brevidens GN=SAMS PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.759e-22
Identity = 29/35 (82.86%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            D+EILKIVKENFDFRPGM+SINLDLKRGGN + L+
Sbjct:  330 DREILKIVKENFDFRPGMMSINLDLKRGGNGRFLK 364

HSP 2 Score: 62.3882 bits (150), Expect = 2.759e-22
Identity = 27/30 (90.00%), Postives = 28/30 (93.33%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*E 181
            GRFLKTAAYGHFGRDD DFTWE+VKPLK E
Sbjct:  360 GRFLKTAAYGHFGRDDADFTWEVVKPLKWE 389

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK5_POPTR (S-adenosylmethionine synthetase 5 OS=Populus trichocarpa GN=METK5 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.590e-22
Identity = 26/27 (96.30%), Postives = 27/27 (100.00%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK 175
            RFLKTAAYGHFGRDDPDFTWE+VKPLK
Sbjct:  362 RFLKTAAYGHFGRDDPDFTWEVVKPLK 388

HSP 2 Score: 62.003 bits (149), Expect = 3.590e-22
Identity = 29/35 (82.86%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL+IVKE+FDFRPGMISINLDLKRGGN + L+
Sbjct:  331 DKEILQIVKESFDFRPGMISINLDLKRGGNSRFLK 365

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK3_POPTR (S-adenosylmethionine synthetase 3 OS=Populus trichocarpa GN=METK3 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.590e-22
Identity = 26/27 (96.30%), Postives = 27/27 (100.00%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK 175
            RFLKTAAYGHFGRDDPDFTWE+VKPLK
Sbjct:  362 RFLKTAAYGHFGRDDPDFTWEVVKPLK 388

HSP 2 Score: 62.003 bits (149), Expect = 3.590e-22
Identity = 29/35 (82.86%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL+IVKE+FDFRPGMISINLDLKRGGN + L+
Sbjct:  331 DKEILQIVKESFDFRPGMISINLDLKRGGNSRFLK 365

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_PEA (S-adenosylmethionine synthetase 1 (Fragment) OS=Pisum sativum GN=SAMS1 PE=2 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 3.595e-22
Identity = 28/31 (90.32%), Postives = 30/31 (96.77%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGR+DPDFTWE+VKPLK EK
Sbjct:  335 GRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 365

HSP 2 Score: 58.9214 bits (141), Expect = 3.595e-22
Identity = 28/35 (80.00%), Postives = 31/35 (88.57%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            D+EILKIVKE FDFRPGMISINLDL RGGN + L+
Sbjct:  305 DREILKIVKETFDFRPGMISINLDLLRGGNGRFLK 339

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_POPDE (S-adenosylmethionine synthetase OS=Populus deltoides GN=METK PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.671e-22
Identity = 26/27 (96.30%), Postives = 27/27 (100.00%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK 175
            RFLKTAAYGHFGRDDPDFTWE+VKPLK
Sbjct:  362 RFLKTAAYGHFGRDDPDFTWEVVKPLK 388

HSP 2 Score: 61.6178 bits (148), Expect = 4.671e-22
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL+IVKE FDFRPGMISINLDLKRGGN + L+
Sbjct:  331 DKEILQIVKERFDFRPGMISINLDLKRGGNSRFLK 365

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_DAUCA (S-adenosylmethionine synthetase 2 OS=Daucus carota GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 4.671e-22
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE FDFRPGMI+INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKETFDFRPGMIAINLDLKRGGNNRFLK 364

HSP 2 Score: 62.003 bits (149), Expect = 4.671e-22
Identity = 27/30 (90.00%), Postives = 28/30 (93.33%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            RFLKTAAYGHFGRDD DFTWE+VKPLK EK
Sbjct:  361 RFLKTAAYGHFGRDDADFTWEVVKPLKWEK 390

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK3_ACTCH (S-adenosylmethionine synthetase 3 (Fragment) OS=Actinidia chinensis GN=SAM3 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 4.679e-22
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 2

Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRFLKTAAYGHFGRDD DFTWE+VKPLK EK
Sbjct:  327 GRFLKTAAYGHFGRDDADFTWEVVKPLKWEK 357

HSP 2 Score: 60.077 bits (144), Expect = 4.679e-22
Identity = 28/35 (80.00%), Postives = 33/35 (94.29%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE+FDFRPGMI+I+LDLKRGGN + L+
Sbjct:  297 DKEILKIVKESFDFRPGMIAIHLDLKRGGNGRFLK 331

[Close]

BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_TRIMO (S-adenosylmethionine synthetase 1 OS=Triticum monococcum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 6.076e-22
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = 1

Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNR 93
            DKEILKIVKENFDFRPGMISINLDLK+GGNR
Sbjct:  332 DKEILKIVKENFDFRPGMISINLDLKKGGNR 362

HSP 2 Score: 59.3066 bits (142), Expect = 6.076e-22
Identity = 25/33 (75.76%), Postives = 29/33 (87.88%), Query Frame = 2

Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            RF+KTAAYGHFGR+D DFTWE+VKPLK +K  A
Sbjct:  362 RFIKTAAYGHFGREDADFTWEVVKPLKFDKASA 394

The following BLAST results are available for this feature:

BLAST of CF504131 vs. ExPASy Swiss-Prot

BLAST of CF504131 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 79


Match Name	E-value	Identity	Description
METK2_BRAJU	2.121e-22	82.35	S-adenosylmethionine synthetase 2 OS=Brassica junc...	[more]
METK2_POPTR	2.759e-22	93.55	S-adenosylmethionine synthetase 2 OS=Populus trich...	[more]
METK_SOLBR	2.759e-22	82.86	S-adenosylmethionine synthetase OS=Solanum brevide...	[more]
METK5_POPTR	3.590e-22	96.30	S-adenosylmethionine synthetase 5 OS=Populus trich...	[more]
METK3_POPTR	3.590e-22	96.30	S-adenosylmethionine synthetase 3 OS=Populus trich...	[more]
METK1_PEA	3.595e-22	90.32	S-adenosylmethionine synthetase 1 (Fragment) OS=Pi...	[more]
METK_POPDE	4.671e-22	96.30	S-adenosylmethionine synthetase OS=Populus deltoid...	[more]
METK2_DAUCA	4.671e-22	82.86	S-adenosylmethionine synthetase 2 OS=Daucus carota...	[more]
METK3_ACTCH	4.679e-22	90.32	S-adenosylmethionine synthetase 3 (Fragment) OS=Ac...	[more]
METK1_TRIMO	6.076e-22	96.77	S-adenosylmethionine synthetase 1 OS=Triticum mono...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_119000-619 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_B11 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504131 ID=CF504131; Name=CF504131; organism=Citrus sinensis; type=EST; length=342bp
GACAAGGAAATTTTGAAGATTGTGAAGGAGAATTTTGATTTTAGGCCTGG
GATGATTTCTATTAATCTTGATCTCAAGAGGGGTGGCAACCGGCAGGTTC
TTGAAGACTGCCGCTTATGGGCACTTTGGCAGAGATGATCCTGATTTCAC
TTGGGAGATTGTTAAGCCACTCAAGTAGGAGAAACTTCCAGCTTAAACTA
ATCAAGGATGAGCTGCTGGCCTCTCATTGGTTCTTATATATGCTTACTTT
TTTTTTTACTTTTGTTTCAGTTACATGAATGTTTTCTTTCTTTCTTTCTT
TTTCTTTTCCTTTTTTTTTTTTTTTATAAATATGTTTAATGG