CF504131

Overview
NameCF504131
Unique NameCF504131
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length342
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF504131_ssr283 microsatellite CF504131_ssr283:283..298. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:167..505. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_GOSHI (S-adenosylmethionine synthetase OS=Gossypium hirsutum GN=SAMS PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 6.076e-22
Identity = 29/35 (82.86%), Postives = 32/35 (91.43%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKE FDFRPGMI+INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKETFDFRPGMIAINLDLKRGGNSRFLK 364

HSP 2 Score: 62.003 bits (149), Expect = 6.076e-22
Identity = 27/33 (81.82%), Postives = 30/33 (90.91%), Query Frame = 2 
Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            RFLKTAAYGHFGRDD DFTWE+VKPLK +K+ A
Sbjct:  361 RFLKTAAYGHFGRDDTDFTWEVVKPLKWDKVHA 393
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_CARPA (S-adenosylmethionine synthetase OS=Carica papaya GN=SAMS PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.891e-22
Identity = 30/35 (85.71%), Postives = 33/35 (94.29%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEILKIVKENFDFRPGMI+INLDLKRGGN + L+
Sbjct:  330 DKEILKIVKENFDFRPGMITINLDLKRGGNSRFLK 364

HSP 2 Score: 59.3066 bits (142), Expect = 7.891e-22
Identity = 24/25 (96.00%), Postives = 25/25 (100.00%), Query Frame = 2 
Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKP 169
            RFLKTAAYGHFGRDDPDFTWE+VKP
Sbjct:  361 RFLKTAAYGHFGRDDPDFTWEVVKP 385
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK3_HORVU (S-adenosylmethionine synthetase 3 OS=Hordeum vulgare GN=SAM3 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 7.905e-22
Identity = 28/31 (90.32%), Postives = 31/31 (100.00%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNR 93
            D+EILK+VKENFDFRPGMISINLDLK+GGNR
Sbjct:  332 DREILKLVKENFDFRPGMISINLDLKKGGNR 362

HSP 2 Score: 60.8474 bits (146), Expect = 7.905e-22
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            RF+KTAAYGHFGRDD DFTWE+VKPLK +K  A
Sbjct:  362 RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_PINCO (S-adenosylmethionine synthetase 2 OS=Pinus contorta GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.028e-21
Identity = 29/34 (85.29%), Postives = 29/34 (85.29%), Query Frame = 2 
Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            GRF KTAAYGHFGRDDPDFTWE VKPLK EK  A
Sbjct:  360 GRFQKTAAYGHFGRDDPDFTWETVKPLKWEKAQA 393

HSP 2 Score: 58.9214 bits (141), Expect = 1.028e-21
Identity = 26/30 (86.67%), Postives = 30/30 (100.00%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGN 90
            DKEIL+I+KE+FDFRPGMI+INLDLKRGGN
Sbjct:  330 DKEILEIIKEHFDFRPGMITINLDLKRGGN 359
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.028e-21
Identity = 27/31 (87.10%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK 184
            GRF KTAAYGHFGRDDPDFTWE+VKPLK +K
Sbjct:  360 GRFQKTAAYGHFGRDDPDFTWEVVKPLKWDK 390

HSP 2 Score: 59.3066 bits (142), Expect = 1.028e-21
Identity = 27/30 (90.00%), Postives = 29/30 (96.67%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGN 90
            DKEILKIVKE FDFRPGM++INLDLKRGGN
Sbjct:  330 DKEILKIVKETFDFRPGMMTINLDLKRGGN 359
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK2_PICSI (S-adenosylmethionine synthetase 2 OS=Picea sitchensis GN=METK2 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.028e-21
Identity = 28/34 (82.35%), Postives = 29/34 (85.29%), Query Frame = 2 
Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            GRF KTAAYGHFGRDDPDFTWE VKPLK +K  A
Sbjct:  360 GRFQKTAAYGHFGRDDPDFTWETVKPLKWDKAQA 393

HSP 2 Score: 60.077 bits (144), Expect = 1.028e-21
Identity = 26/30 (86.67%), Postives = 30/30 (100.00%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGN 90
            DKEIL+++KENFDFRPGMI+INLDLKRGGN
Sbjct:  330 DKEILELIKENFDFRPGMITINLDLKRGGN 359
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_MEDFA (S-adenosylmethionine synthetase OS=Medicago falcata GN=SAMS PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.740e-21
Identity = 29/35 (82.86%), Postives = 31/35 (88.57%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNRQVLE 105
            DKEIL IVKE FDFRPGMISINLDLKRGGN + L+
Sbjct:  332 DKEILNIVKETFDFRPGMISINLDLKRGGNNRFLK 366

HSP 2 Score: 60.8474 bits (146), Expect = 1.740e-21
Identity = 27/31 (87.10%), Postives = 28/31 (90.32%), Query Frame = 2 
Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EKL 187
            RFLKTAAYGHFGRDD DFTWE+VKPLK  KL
Sbjct:  363 RFLKTAAYGHFGRDDADFTWEVVKPLKGGKL 393
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK3_PICSI (S-adenosylmethionine synthetase 3 OS=Picea sitchensis GN=METK3 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.741e-21
Identity = 32/35 (91.43%), Postives = 32/35 (91.43%), Query Frame = 2 
Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EK-LPA 193
            GRF KTAAYGHFGRDDPDFTWEIVKPLK EK LPA
Sbjct:  360 GRFQKTAAYGHFGRDDPDFTWEIVKPLKQEKALPA 394

HSP 2 Score: 55.8398 bits (133), Expect = 1.741e-21
Identity = 24/30 (80.00%), Postives = 29/30 (96.67%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGN 90
            DK IL+++K+NFDFRPGMI+INLDLKRGGN
Sbjct:  330 DKVILELIKKNFDFRPGMITINLDLKRGGN 359
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.741e-21
Identity = 27/31 (87.10%), Postives = 31/31 (100.00%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGNR 93
            D+EILK+VKENFDFRPGMI+INLDLK+GGNR
Sbjct:  332 DREILKLVKENFDFRPGMITINLDLKKGGNR 362

HSP 2 Score: 60.8474 bits (146), Expect = 1.741e-21
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:   95 RFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            RF+KTAAYGHFGRDD DFTWE+VKPLK +K  A
Sbjct:  362 RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394
[Close]
BLAST of CF504131 vs. ExPASy Swiss-Prot
Match: METK_PINBN (S-adenosylmethionine synthetase OS=Pinus banksiana GN=METK PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.832e-21
Identity = 28/34 (82.35%), Postives = 28/34 (82.35%), Query Frame = 2 
Query:   92 GRFLKTAAYGHFGRDDPDFTWEIVKPLK*EKLPA 193
            GR  KTAAYGHFGRDDPDFTWE VKPLK EK  A
Sbjct:  360 GRSQKTAAYGHFGRDDPDFTWETVKPLKWEKAQA 393

HSP 2 Score: 60.077 bits (144), Expect = 3.832e-21
Identity = 27/30 (90.00%), Postives = 30/30 (100.00%), Query Frame = 1 
Query:    1 DKEILKIVKENFDFRPGMISINLDLKRGGN 90
            DKEIL+I+KE+FDFRPGMISINLDLKRGGN
Sbjct:  330 DKEILEIIKEHFDFRPGMISINLDLKRGGN 359
The following BLAST results are available for this feature:
BLAST of CF504131 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 79
Match NameE-valueIdentityDescription
METK_GOSHI6.076e-2282.86S-adenosylmethionine synthetase OS=Gossypium hirsu... [more]
METK_CARPA7.891e-2285.71S-adenosylmethionine synthetase OS=Carica papaya G... [more]
METK3_HORVU7.905e-2290.32S-adenosylmethionine synthetase 3 OS=Hordeum vulga... [more]
METK2_PINCO1.028e-2185.29S-adenosylmethionine synthetase 2 OS=Pinus contort... [more]
METK2_ARATH1.028e-2187.10S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK2_PICSI1.028e-2182.35S-adenosylmethionine synthetase 2 OS=Picea sitchen... [more]
METK_MEDFA1.740e-2182.86S-adenosylmethionine synthetase OS=Medicago falcat... [more]
METK3_PICSI1.741e-2191.43S-adenosylmethionine synthetase 3 OS=Picea sitchen... [more]
METK1_HORVU1.741e-2187.10S-adenosylmethionine synthetase 1 OS=Hordeum vulga... [more]
METK_PINBN3.832e-2182.35S-adenosylmethionine synthetase OS=Pinus banksiana... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-619 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_B11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504131 ID=CF504131; Name=CF504131; organism=Citrus sinensis; type=EST; length=342bp
GACAAGGAAATTTTGAAGATTGTGAAGGAGAATTTTGATTTTAGGCCTGG
GATGATTTCTATTAATCTTGATCTCAAGAGGGGTGGCAACCGGCAGGTTC
TTGAAGACTGCCGCTTATGGGCACTTTGGCAGAGATGATCCTGATTTCAC
TTGGGAGATTGTTAAGCCACTCAAGTAGGAGAAACTTCCAGCTTAAACTA
ATCAAGGATGAGCTGCTGGCCTCTCATTGGTTCTTATATATGCTTACTTT
TTTTTTTACTTTTGTTTCAGTTACATGAATGTTTTCTTTCTTTCTTTCTT
TTTCTTTTCCTTTTTTTTTTTTTTTATAAATATGTTTAATGG
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