CF832309 | Citrus Genome Database

Overview


Name	CF832309
Unique Name	CF832309
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	767

Libraries


Library Name	Type
Ruby Orange Ovary at Anthesis cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig5773	contig	Csv1_Contig5773:483..1249.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.417e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 3

Query:  144 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 142.51 bits (358), Expect = 2.417e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 3

Query:  372 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 90.8929 bits (224), Expect = 8.347e-18
Identity = 43/45 (95.56%), Postives = 45/45 (100.00%), Query Frame = 3

Query:    9 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 143
            DKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   32 DKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.776e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 3

Query:  144 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 137.502 bits (345), Expect = 7.776e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 3

Query:  372 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 88.1965 bits (217), Expect = 5.410e-17
Identity = 42/45 (93.33%), Postives = 44/45 (97.78%), Query Frame = 3

Query:    9 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 143
            DKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   32 DKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 3.058e-28
Identity = 61/77 (79.22%), Postives = 68/77 (88.31%), Query Frame = 3

Query:  372 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGD 602
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG+
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGGN 77

HSP 2 Score: 125.176 bits (313), Expect = 3.994e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 3

Query:  144 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 83.9593 bits (206), Expect = 1.020e-15
Identity = 39/45 (86.67%), Postives = 43/45 (95.56%), Query Frame = 3

Query:    9 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 143
            DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:   32 DKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 2.589e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 3

Query:  144 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 122.479 bits (306), Expect = 2.589e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 3

Query:  372 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 84.7297 bits (208), Expect = 5.982e-16
Identity = 39/45 (86.67%), Postives = 43/45 (95.56%), Query Frame = 3

Query:    9 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 143
            DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:   32 DKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 9.837e-27
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 3

Query:  177 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 365
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 120.553 bits (301), Expect = 9.837e-27
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 3

Query:  405 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 593
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 3 Score: 87.8113 bits (216), Expect = 7.066e-17
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = 3

Query:    9 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 137
            DKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:   21 DKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 8.066e-21
Identity = 46/77 (59.74%), Postives = 60/77 (77.92%), Query Frame = 3

Query:  372 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGD 602
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGGD
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGD 77

HSP 2 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 3

Query:  144 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 380
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 3 Score: 68.1662 bits (165), Expect = 5.794e-11
Identity = 32/48 (66.67%), Postives = 37/48 (77.08%), Query Frame = 3

Query:    9 DKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 152
            +KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:   32 EKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.347e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  150 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 99.3673 bits (246), Expect = 2.347e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  378 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  150 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  378 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  150 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  378 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of CF832309 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  150 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 371
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.065e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 3

Query:  378 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 599
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

The following BLAST results are available for this feature:

BLAST of CF832309 vs. ExPASy Swiss-Prot

BLAST of CF832309 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99


Match Name	E-value	Identity	Description
UBIQ_LEIMA	2.417e-33	93.42	Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1	[more]
UBIQ_LEITA	7.776e-32	90.79	Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3...	[more]
UBIL_NPVOP	3.058e-28	79.22	Ubiquitin-like protein OS=Orgyia pseudotsugata mul...	[more]
UBIL_NPVAC	2.589e-27	76.32	Ubiquitin-like protein OS=Autographa californica n...	[more]
UBIQ_LUMTE	9.837e-27	95.24	Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ...	[more]
NEDD8_DROME	8.066e-21	59.74	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]
RUB1_DESAN	2.347e-20	64.86	NEDD8-like protein RUB1 OS=Deschampsia antarctica ...	[more]
RUB2_ORYSJ	3.065e-20	63.51	NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap...	[more]
RUB1_ORYSJ	3.065e-20	63.51	NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap...	[more]
RUB1_ARATH	3.065e-20	63.51	NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS02_01K13_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01K13, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF832309 ID=CF832309; Name=CF832309; organism=Citrus sinensis; type=EST; length=767bp
GACGCCATGACAAGGAAGGGATTCCACCGGACCAGCAGCGGCTGATCTTC
GCCGGAAAGCAGCTCGAGGACGGGCGCACCTTGGCGGATTACAACATCCA
GAAGGAGTCGACTCTCCACCTCGTCCTCCGTCTCCGCGGCGGCATGCAGA
TCTTCGTCAAGACTCTGACCGGGAAGACGATCACCCTGGAGGTGGAGAGC
TCCGACACGATCGACAACGTGAAGGCGAAGATCCAGGACAAGGAAGGGAT
CCCCCCGGACCAGCAGAGGCTCATCTTCGCCGGCAAGCAGCTGGAGGACG
GCCGCACCCTGGCCGATTATAACATCCAGAAGGAATCCACGCTCCACCTT
GTGTTGCGCCTGAGGGGAGGAATGCAGATCTTCGTCAAGACGTTGACCGG
GAAGACGATTACCCTGGAGGTGGAGAGCTCCGACACGATCGACAACGTGA
AGGCCAAGATCCAGGACAAAGAGGGGATTCCTCCGGACCAGCAGAGGCTC
ATCTTCGCCGGCAAGCAGCTCGAGGATGGCCGGACTCTCGCCGATTACAA
CATTCAAAAGGAGTCCACTCTCCACCTTGTCCTCCGTCTCCGTGGTGGTG
ACTTCTAAAAAAATATATGTGGATGTGTCCGCGTTTGATTGGATGGCGCT
AATAATAATTAATTAGGTTGGATTGGGCGCTTTGCTTTAATTATTTTGTA
ATTACAATTGTGTAATGGCCAGCCGCTTGGGCCTAATTTCCTCTTTCTAT
CAGCAATAATAATATAA