CF832741

Overview
NameCF832741
Unique NameCF832741
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACME (Xylose isomerase OS=Bacillus megaterium GN=xylA PE=3 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 5.673e-31
Identity = 67/144 (46.53%), Postives = 90/144 (62.50%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G++DAN GDP +GWDTD+F TD+   TL M  +++NGGL  GG NFDAK+RR S + +DL  AH+ GMD  ARGL+ A KL+ED     ++ +RY SF   IG +I  GKA+F  LE+ A +       S +QE  + I    I
Sbjct:  299 GSVDANQGDPLLGWDTDEFPTDLYSTTLAMYEILQNGGLGSGGLNFDAKVRRGSFEQDDLLYAHVAGMDAFARGLKVAHKLVEDRVFENVINERYSSFKEGIGLEIVEGKANFHTLEQYAFKNPNIANKSGRQERLKSILNQYI 442          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_PEDPA (Xylose isomerase OS=Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) GN=xylA PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 7.409e-31
Identity = 66/117 (56.41%), Postives = 80/117 (68.38%), Query Frame = -3
Query:  354 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLE 704
            G++DAN GD  +GWD D+F  DI EATL+M  ++KNGGLA GG NFDAK RR+S  +EDLF+AH  GMDT A GLR AAKL+ED     ++  RY SF S IG   E  K  F+ LE
Sbjct:  294 GSLDANMGDKLIGWDIDEFPNDIYEATLVMYEMLKNGGLATGGLNFDAKPRRQSFTMEDLFLAHTAGMDTYAAGLRVAAKLLEDRVFDSVIEDRYSSFKSGIGADFENDKVTFKELE 410          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_HAEI8 (Xylose isomerase OS=Haemophilus influenzae (strain 86-028NP) GN=xylA PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 7.409e-31
Identity = 67/145 (46.21%), Postives = 93/145 (64.14%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAEMIFQSAI 704
            G+IDAN GDPQ+GWDTDQF   + E TL+M  ++K+GG   GGFNFDAK+RR+S D  DLF AHI  +D +A  L+ AAK++++ +L ++V  RY  ++SE+GQ I  GK   E L +   +    PK  S +QE  E +    I
Sbjct:  293 GSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKHGGFTTGGFNFDAKIRRQSIDPYDLFYAHIGAIDVLALSLKRAAKMLQEKTLQKIVNARYAGWNSELGQHILQGKTSLETLAQLVQQKDLAPKPVSGQQEYLENLVNQVI 437          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACC1 (Xylose isomerase OS=Bacillus cereus (strain ATCC 10987) GN=xylA PE=3 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 7.409e-31
Identity = 68/144 (47.22%), Postives = 92/144 (63.89%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G++DAN G+P +GWDTD+F T++   TL M  +++NGGL  GG NFDAK+RR S + EDL  AHI GMD  ARGL+ A KL+ED     ++ +RY SF   IG +I  G+A+F  LE+ A++    K  S KQE  + I    I
Sbjct:  299 GSVDANQGNPLLGWDTDEFPTNLYSTTLAMYEILQNGGLGSGGLNFDAKVRRASFEEEDLVYAHIAGMDAFARGLKVAHKLLEDRVFENIIDERYHSFRDGIGLEIVEGRANFHTLEQYALQNSIIKNQSGKQERLKAILNQYI 442          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_LISW6 (Xylose isomerase OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=xylA PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.264e-30
Identity = 64/121 (52.89%), Postives = 87/121 (71.90%), Query Frame = -3
Query:  342 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAM 704
            G+IDAN GD  +GWDTD+F T+I + TL M  +++NGG+APGG NFDAK+RR S ++EDL +AHI GMDT ARGL+ AAKL ED    ++  +RY+SF   IG +I   + DF+ L + A+
Sbjct:  291 GSIDANQGDLLLGWDTDEFPTNIYDTTLTMYEILQNGGIAPGGINFDAKVRRTSFEMEDLLLAHIAGMDTYARGLKAAAKLTEDHFFDKIKEERYRSFKKGIGARILDNQEDFKSLTEYAL 411          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACP2 (Xylose isomerase OS=Bacillus pumilus (strain SAFR-032) GN=xylA PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.264e-30
Identity = 63/134 (47.01%), Postives = 92/134 (68.66%), Query Frame = -3
Query:  303 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQE 704
            G++DAN G+  +GWDTD+F TD+   TL M  +++NGGL  GG NFDAK+RR S ++ED+  AHI GMD  A+GLR A +LIED    ++++ RY+SF   IG+ I  GKA+F +LE+ A++    +  S ++E
Sbjct:  299 GSVDANQGNTLLGWDTDEFPTDLYATTLAMYEILQNGGLGTGGLNFDAKVRRASFELEDILYAHIAGMDAFAKGLRVAHRLIEDRVFEDVIKHRYRSFSEGIGRDISEGKANFHILEEYALKHPLIRNESGREE 432          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_TETHA (Xylose isomerase OS=Tetragenococcus halophilus GN=xylA PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.651e-30
Identity = 65/121 (53.72%), Postives = 84/121 (69.42%), Query Frame = -3
Query:  342 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAM 704
            G++DAN GD  +GWDTD+F TDI  ATL M  V+  GG+APGG NFDAK+RR S  ++DL +AHI GMDT ARGLR AAK+ +D    +++  RY+SF S IG++I   K D E L   A+
Sbjct:  291 GSLDANQGDLLLGWDTDEFPTDIYTATLAMYEVLDFGGIAPGGLNFDAKVRRTSFAMDDLILAHIAGMDTYARGLRAAAKMKDDNFFEQIIANRYESFSSGIGKQIVENKEDLESLTNYAL 411          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_RHOBA (Xylose isomerase OS=Rhodopirellula baltica GN=xylA PE=3 SV=2)

HSP 1 Score: 132.88 bits (333), Expect = 1.651e-30
Identity = 72/145 (49.66%), Postives = 92/145 (63.45%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKV-PSAKQELAEMIFQSAI 704
            G+IDANTGD  +GWDTDQF TD    T  ML ++K+GG+  GG NFDAK+RRES +  DLF AHI GMD  A+GL+ AA +   G L + V+ RY ++DS IG KIEAG+  F  LE   +E G+     S +QE  E +    I
Sbjct:  292 GSIDANTGDLLLGWDTDQFPTDYYLTTQTMLMILKHGGIGTGGVNFDAKVRRESFEPIDLFHAHIGGMDAFAKGLKIAAAIRASGELADFVKNRYSTWDSGIGAKIEAGEVGFAELEAYMLEKGDVDANQSGRQEYLEHMINKYI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA1_XANCP (Xylose isomerase 1 OS=Xanthomonas campestris pv. campestris GN=xylA1 PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 2.156e-30
Identity = 71/137 (51.82%), Postives = 85/137 (62.04%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAE 704
            G+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+    L     +RY SFDS  G     G +    L K A   GEP   S +QE  E
Sbjct:  301 GSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVADALLTSSPLETWRAQRYASFDSGAGADFANGTSTLADLAKYAAGRGEPTQVSGRQEAYE 437          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA1_XANC8 (Xylose isomerase 1 OS=Xanthomonas campestris pv. campestris (strain 8004) GN=xylA1 PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 2.156e-30
Identity = 71/137 (51.82%), Postives = 85/137 (62.04%), Query Frame = -3
Query:  294 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAE 704
            G+IDAN G+PQ GWDTDQF TD+ +    ML V++ GGLAPGG NFDAK+RRES+D +DLF+AHI GMD  ARGL  A  L+    L     +RY SFDS  G     G +    L K A   GEP   S +QE  E
Sbjct:  301 GSIDANRGNPQNGWDTDQFPTDLYDTVGAMLVVLRQGGLAPGGLNFDAKVRRESSDPQDLFLAHIGGMDAFARGLEVADALLTSSPLETWRAQRYASFDSGAGADFANGTSTLADLAKYAAGRGEPTQVSGRQEAYE 437          
The following BLAST results are available for this feature:
BLAST of CF832741 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 172
Match NameE-valueIdentityDescription
XYLA_BACME5.673e-3146.53Xylose isomerase OS=Bacillus megaterium GN=xylA PE... [more]
XYLA_PEDPA7.409e-3156.41Xylose isomerase OS=Pediococcus pentosaceus (strai... [more]
XYLA_HAEI87.409e-3146.21Xylose isomerase OS=Haemophilus influenzae (strain... [more]
XYLA_BACC17.409e-3147.22Xylose isomerase OS=Bacillus cereus (strain ATCC 1... [more]
XYLA_LISW61.264e-3052.89Xylose isomerase OS=Listeria welshimeri serovar 6b... [more]
XYLA_BACP21.264e-3047.01Xylose isomerase OS=Bacillus pumilus (strain SAFR-... [more]
XYLA_TETHA1.651e-3053.72Xylose isomerase OS=Tetragenococcus halophilus GN=... [more]
XYLA_RHOBA1.651e-3049.66Xylose isomerase OS=Rhodopirellula baltica GN=xylA... [more]
XYLA1_XANCP2.156e-3051.82Xylose isomerase 1 OS=Xanthomonas campestris pv. c... [more]
XYLA1_XANC82.156e-3051.82Xylose isomerase 1 OS=Xanthomonas campestris pv. c... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02F15_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02F15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832741 ID=CF832741; Name=CF832741; organism=Citrus sinensis; type=EST; length=706bp
CTTTTTTTTTTTTCCAAATTGAACTTCGATCATTATTAAAGCAATGTTAT
TGACAAAAGATTTCAAGATCGTAGGATTCAAGTTTTATAACAGCTGTCAT
ATCGATAACTAAACAAGTTCTTTATAAAGCCTAAGCTAGACAAACATGTC
AAAATTTCCCCGCATGAAAAGACCCATTTCCCCAAGAGGGTTCACTGACT
GGAGACAAACATAACTTCAATTTGCCCCTCTCTCTTTATTTGTAAGTTCT
GCTTCGGAGCAGCCGCTATCTATATTGCAGATTGGAAGATCATTTCTGCA
AGTTCCTGCTTAGCAGAGGGAACCTTGGGTTCTCCCCATTCCATGGCCTT
CTTCTCAAGCATTTCAAAATCAGCCTTACCAGCCTCTATTTTTTGCCCAA
TCTCTGAGTCAAAGCTCTGGTATCGCTTGCGGACAAGCTCCGGTAGAGAA
CCATCCTCAATCAGCTTGGCAGCAGTTCGAAGTCCACGAGCAACGGTATC
CATTCCAACAATATGAGCAATGAACAAGTCTTCAACGTCAGTGCTCTCTC
TTCGTAATTTTGCATCAAAGTTGAATCCTCCAGGTGCCAAACCTCCATTT
TTAATCACACTGAGCATAATCAAAGTAGCCTCTCCAATATCTGTCAGGAA
TTGATCTGTATCCCAACCAACTTGAGGGTCACCAGTGTTTGCATCGATGT
TTCCTA
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