CK740326

Overview
NameCK740326
Unique NameCK740326
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length463
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2264 contig Csv1_Contig2264:281..742. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2D2_PIG (Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.526e-25
Identity = 48/91 (52.75%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP V F +R++HPNI ++GSICLDIL++QWSP   ++ +L SI SLLCDPNP+ P   E AR++  ++ +Y+R  RE
Sbjct:   50 FFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISRE 140

HSP 2 Score: 29.261 bits (64), Expect = 1.526e-25
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP+D+P+ GG
Sbjct:   25 PVGDDMFHWQATIMGPNDSPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBCD1_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=eff PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.986e-25
Identity = 47/91 (51.65%), Postives = 65/91 (71.43%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP V F +R++HPNI ++GSICLDIL++QWSP   ++ +L SI SLLCDPNP+ P   E AR++  ++ +Y+   RE
Sbjct:   50 FFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELARE 140

HSP 2 Score: 28.8758 bits (63), Expect = 1.986e-25
Identity = 10/24 (41.67%), Postives = 14/24 (58.33%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP D+P+ GG
Sbjct:   25 PVGDDLFHWQATIMGPPDSPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.585e-25
Identity = 45/91 (49.45%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  +YP KPP V F +R++HPN+ ++GSICLDIL++QWSP   ++ +L SI SLLCDPNP+ P   E A+++  ++ +Y+R  RE
Sbjct:   50 FFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTARE 140

HSP 2 Score: 28.8758 bits (63), Expect = 2.585e-25
Identity = 10/24 (41.67%), Postives = 14/24 (58.33%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P    +  W A I GP+D+P+ GG
Sbjct:   25 PVGEDMFHWQATIMGPNDSPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.585e-25
Identity = 45/91 (49.45%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  +YP KPP V F +R++HPN+ ++GSICLDIL++QWSP   ++ +L SI SLLCDPNP+ P   E A+++  ++ +Y+R  RE
Sbjct:   50 FFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTARE 140

HSP 2 Score: 28.8758 bits (63), Expect = 2.585e-25
Identity = 10/24 (41.67%), Postives = 14/24 (58.33%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P    +  W A I GP+D+P+ GG
Sbjct:   25 PVGEDMFHWQATIMGPNDSPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 3.364e-25
Identity = 47/91 (51.65%), Postives = 64/91 (70.33%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP V F ++++HPNI ++GSICLDIL++QWSP   V+ +L SI SLLCDPNP+ P   E A  +  ++ +Y+R  RE
Sbjct:   50 FFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLARE 140

HSP 2 Score: 29.6462 bits (65), Expect = 3.364e-25
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP+D+P+ GG
Sbjct:   25 PVGDDLFHWQATIMGPNDSPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UBC2_CAEEL (Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditis elegans GN=let-70 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 5.700e-25
Identity = 47/91 (51.65%), Postives = 65/91 (71.43%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP V F +R++HPNI ++GSICLDIL++QWSP   ++ +L SI SLLCDPNP+ P   E AR++  ++  Y++  RE
Sbjct:   50 FFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRERYNQLARE 140

HSP 2 Score: 27.335 bits (59), Expect = 5.700e-25
Identity = 9/24 (37.50%), Postives = 14/24 (58.33%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP ++P+ GG
Sbjct:   25 PVGDDLFHWQATIMGPPESPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 9.655e-25
Identity = 47/91 (51.65%), Postives = 65/91 (71.43%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP V F +R++HPNI ++ SICLDIL++QWSP   ++ +L SI SLLCDPNP+ P   E AR++  ++ +Y+R  RE
Sbjct:   50 FFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISRE 140

HSP 2 Score: 29.261 bits (64), Expect = 9.655e-25
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP+D+P+ GG
Sbjct:   25 PVGDDMFHWQATIMGPNDSPYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2D1_MOUSE (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus GN=Ube2d1 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.636e-24
Identity = 46/91 (50.55%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP + F ++++HPNI ++GSICLDIL++QWSP   V+ +L SI SLLCDPNP+ P   + A+++  +K +Y+R  RE
Sbjct:   50 FFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHARE 140

HSP 2 Score: 25.7942 bits (55), Expect = 1.636e-24
Identity = 9/24 (37.50%), Postives = 13/24 (54.17%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP D+ + GG
Sbjct:   25 PVGDDLFHWQATIMGPPDSAYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2D1_HUMAN (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.636e-24
Identity = 46/91 (50.55%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP + F ++++HPNI ++GSICLDIL++QWSP   V+ +L SI SLLCDPNP+ P   + A+++  +K +Y+R  RE
Sbjct:   50 FFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHARE 140

HSP 2 Score: 25.7942 bits (55), Expect = 1.636e-24
Identity = 9/24 (37.50%), Postives = 13/24 (54.17%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP D+ + GG
Sbjct:   25 PVGDDLFHWQATIMGPPDSAYQGG 48
[Close]
BLAST of CK740326 vs. ExPASy Swiss-Prot
Match: UB2D1_BOVIN (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.636e-24
Identity = 46/91 (50.55%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   85 FKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYHRRVRE 357
            F LT+ F  DYP KPP + F ++++HPNI ++GSICLDIL++QWSP   V+ +L SI SLLCDPNP+ P   + A+++  +K +Y+R  RE
Sbjct:   50 FFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHARE 140

HSP 2 Score: 25.7942 bits (55), Expect = 1.636e-24
Identity = 9/24 (37.50%), Postives = 13/24 (54.17%), Query Frame = 3 
Query:    9 PQDNSIMLWNAVIFGPDDTPWDGG 80
            P  + +  W A I GP D+ + GG
Sbjct:   25 PVGDDLFHWQATIMGPPDSAYQGG 48
The following BLAST results are available for this feature:
BLAST of CK740326 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
UB2D2_PIG1.526e-2552.75Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa G... [more]
UBCD1_DROME1.986e-2551.65Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi... [more]
U2D2B_RAT2.585e-2549.45Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv... [more]
U2D2B_MOUSE2.585e-2549.45Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu... [more]
UB2D4_HUMAN3.364e-2551.65Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]
UBC2_CAEEL5.700e-2551.65Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditi... [more]
UB2D3_BOVIN9.655e-2551.65Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G... [more]
UB2D1_MOUSE1.636e-2450.55Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus... [more]
UB2D1_HUMAN1.636e-2450.55Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens... [more]
UB2D1_BOVIN1.636e-2450.55Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus G... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6470 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE63H07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK740326 ID=CK740326; Name=CK740326; organism=Citrus sinensis; type=EST; length=463bp
GTGGTGCTCCTCAGGACAACAGTATAATGCTTTGGAATGCTGTTATATTC
GGGCCGGATGACACCCCATGGGATGGAGGTACCGTTTAAATTGACACTTC
AGTTCACAGAGGATTATCCAAATAAGCCACCAACAGTGCGCTTTGTTTCT
CGGATGTTCCATCCAAATATTTATGCGGATGGAAGCATTTGCTTGGACAT
TCTACAAAATCAGTGGAGTCCAATTTACGATGTAGCAGCAATACTCACGT
CAATTCAGTCATTGCTTTGTGACCCAAATCCAAACTCTCCTGCAAATTCG
GAGGCAGCACGGATGTTTAGCGAGAACAAGCGTGAATACCATAGGAGAGT
ACGGGAAATTGTTGAGCAGAGTTGGACTGCTGACTAGTGGCAACTTGTTG
GAATGTAGGGAACTTCAAAAATAGTCCAATGGCTTGTTCTAAATAACATT
TAAGATTCAAAAG
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