EY755965

Overview
NameEY755965
Unique NameEY755965
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length647
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5506 contig Csv1_Contig5506:35..683. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC1_BRAJA (Fumarate hydratase class II 1 OS=Bradyrhizobium japonicum GN=fumC1 PE=3 SV=3)

HSP 1 Score: 87.4261 bits (215), Expect = 3.136e-30
Identity = 43/66 (65.15%), Postives = 52/66 (78.79%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            +IS+L+  SLMLVT+L PKIGYDNAA VAK AH  GTTLK+ AL+LG + ++EFD LV PEKM  P
Sbjct:  407 RISELMQRSLMLVTALAPKIGYDNAAKVAKTAHANGTTLKEEALRLGFVTADEFDRLVQPEKMTKP 472

HSP 2 Score: 65.855 bits (159), Expect = 3.136e-30
Identity = 28/49 (57.14%), Postives = 40/49 (81.63%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            V GS GHFELNV+KP++A  +LHS+RL+ DA+ SF ++CV GI+A+ +R
Sbjct:  359 VAGSQGHFELNVYKPVLAYNMLHSIRLMADAARSFTEHCVSGIRADEKR 407
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMH_CAEEL (Probable fumarate hydratase, mitochondrial OS=Caenorhabditis elegans GN=fum-1 PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 5.312e-30
Identity = 41/65 (63.08%), Postives = 50/65 (76.92%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I+K++ ESLMLVT+LNP IGYDNAA +AK AHK GTTL   A+KLG+L  E+F   V PE M+GP
Sbjct:  436 IAKIMRESLMLVTALNPHIGYDNAAKIAKTAHKNGTTLVQEAVKLGILTEEQFAQWVKPENMLGP 500

HSP 2 Score: 65.855 bits (159), Expect = 5.312e-30
Identity = 30/48 (62.50%), Postives = 37/48 (77.08%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            VGGSNGHFELNVFKP+I   +L S RLL D++ SF  +CV GI AN++
Sbjct:  387 VGGSNGHFELNVFKPLIVRNVLQSTRLLADSAVSFTDHCVDGIVANKD 434
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_METEA (Fumarate hydratase class II OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=fumC PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.590e-29
Identity = 40/67 (59.70%), Postives = 50/67 (74.63%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGPS 346
            +IS L+  SLMLVT+L P IGYD AA +AK AHK GTTLK+ AL+LG +  EEF+ +V PE M+ PS
Sbjct:  404 RISDLMSRSLMLVTALAPSIGYDKAAEIAKTAHKNGTTLKEEALRLGYVTDEEFERVVRPETMLAPS 470

HSP 2 Score: 67.3958 bits (163), Expect = 2.590e-29
Identity = 31/47 (65.96%), Postives = 38/47 (80.85%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            GS GHFELNVFKP+IA+ +L S+R+L DAS SF  NCV GI+AN +R
Sbjct:  358 GSQGHFELNVFKPVIANAVLQSVRILADASVSFTDNCVVGIKANTDR 404
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMH_YEAST (Fumarate hydratase, mitochondrial OS=Saccharomyces cerevisiae GN=FUM1 PE=1 SV=2)

HSP 1 Score: 90.1225 bits (222), Expect = 4.389e-29
Identity = 44/66 (66.67%), Postives = 55/66 (83.33%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            +I +LL +SLMLVT+LNPKIGYD A+ VAK AHK+G TLK++AL+LGVL  +EFD  VVPE M+GP
Sbjct:  422 RIHELLTKSLMLVTALNPKIGYDAASKVAKNAHKKGITLKESALELGVLTEKEFDEWVVPEHMLGP 487

HSP 2 Score: 59.3066 bits (142), Expect = 4.389e-29
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPNCCT 167
            GS G FELNVFKP++ + LL+S+RL+ DA+ SF  +CV GI+AN  R     T
Sbjct:  376 GSQGQFELNVFKPVMIANLLNSIRLITDAAYSFRVHCVEGIKANEPRIHELLT 428
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_RALSO (Fumarate hydratase class II OS=Ralstonia solanacearum GN=fumC PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.446e-29
Identity = 39/65 (60.00%), Postives = 54/65 (83.08%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I++L+  SLMLVT+LNP IGYD +A +AKKAHKEGT+L++AAL LG + +E+FD  V PE+M+G
Sbjct:  396 RIAELVQRSLMLVTALNPHIGYDKSAQIAKKAHKEGTSLREAALALGYVTAEQFDAWVRPEQMVG 460

HSP 2 Score: 64.3142 bits (155), Expect = 7.446e-29
Identity = 29/49 (59.18%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +GG++G+FELNVF+PMIA   LHS RLL D   SF  +C  GI+ NRER
Sbjct:  348 IGGASGNFELNVFRPMIAYNFLHSARLLADGMRSFNDHCAVGIEPNRER 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_SHIFL (Fumarate hydratase class II OS=Shigella flexneri GN=fumC PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.643e-28
Identity = 43/65 (66.15%), Postives = 53/65 (81.54%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I++LL+ESLMLVT+LN  IGYD AA +AKKAHKEG TLK AAL LG L+  EFD+ V PE+M+G
Sbjct:  397 RINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEFDSWVRPEQMVG 461

HSP 2 Score: 61.6178 bits (148), Expect = 1.643e-28
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +GG++G+FELNVF+PM+    L S+RLL D   SF K+C  GI+ NRER
Sbjct:  349 MGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIEPNRER 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_ECOLI (Fumarate hydratase class II OS=Escherichia coli (strain K12) GN=fumC PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.643e-28
Identity = 43/65 (66.15%), Postives = 53/65 (81.54%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I++LL+ESLMLVT+LN  IGYD AA +AKKAHKEG TLK AAL LG L+  EFD+ V PE+M+G
Sbjct:  397 RINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEFDSWVRPEQMVG 461

HSP 2 Score: 61.6178 bits (148), Expect = 1.643e-28
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +GG++G+FELNVF+PM+    L S+RLL D   SF K+C  GI+ NRER
Sbjct:  349 MGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIEPNRER 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_ECO57 (Fumarate hydratase class II OS=Escherichia coli O157:H7 GN=fumC PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.643e-28
Identity = 43/65 (66.15%), Postives = 53/65 (81.54%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I++LL+ESLMLVT+LN  IGYD AA +AKKAHKEG TLK AAL LG L+  EFD+ V PE+M+G
Sbjct:  397 RINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEFDSWVRPEQMVG 461

HSP 2 Score: 61.6178 bits (148), Expect = 1.643e-28
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +GG++G+FELNVF+PM+    L S+RLL D   SF K+C  GI+ NRER
Sbjct:  349 MGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIEPNRER 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_ECOL6 (Fumarate hydratase class II OS=Escherichia coli O6 GN=fumC PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.139e-28
Identity = 43/65 (66.15%), Postives = 52/65 (80.00%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I++LL+ESLMLVT+LN  IGYD AA +AKKAHKEG TLK AAL LG L+  EFD  V PE+M+G
Sbjct:  397 RINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEFDRWVRPEQMVG 461

HSP 2 Score: 61.6178 bits (148), Expect = 2.139e-28
Identity = 27/49 (55.10%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +GG++G+FELNVF+PM+    L S+RLL D   SF K+C  GI+ NRER
Sbjct:  349 MGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIEPNRER 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_HELPY (Fumarate hydratase class II OS=Helicobacter pylori GN=fumC PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.139e-28
Identity = 42/66 (63.64%), Postives = 55/66 (83.33%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            KI   LH SLMLVT+LNP +GY+NAA +AK AHK+G +LK++AL+L +L++E+FD  VVPEKMIGP
Sbjct:  396 KIDYYLHHSLMLVTALNPHVGYENAAKIAKNAHKKGISLKESALELKLLSAEDFDKFVVPEKMIGP 461

HSP 2 Score: 56.225 bits (134), Expect = 2.139e-28
Identity = 26/49 (53.06%), Postives = 33/49 (67.35%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +  S G+FELNVFKP+I    L SLRLL D+  SF  +C  GI+ NRE+
Sbjct:  348 IAASQGNFELNVFKPVIIYNFLQSLRLLSDSMESFNIHCASGIEPNREK 396
The following BLAST results are available for this feature:
BLAST of EY755965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 97
Match NameE-valueIdentityDescription
FUMC1_BRAJA3.136e-3065.15Fumarate hydratase class II 1 OS=Bradyrhizobium ja... [more]
FUMH_CAEEL5.312e-3063.08Probable fumarate hydratase, mitochondrial OS=Caen... [more]
FUMC_METEA2.590e-2959.70Fumarate hydratase class II OS=Methylobacterium ex... [more]
FUMH_YEAST4.389e-2966.67Fumarate hydratase, mitochondrial OS=Saccharomyces... [more]
FUMC_RALSO7.446e-2960.00Fumarate hydratase class II OS=Ralstonia solanacea... [more]
FUMC_SHIFL1.643e-2866.15Fumarate hydratase class II OS=Shigella flexneri G... [more]
FUMC_ECOLI1.643e-2866.15Fumarate hydratase class II OS=Escherichia coli (s... [more]
FUMC_ECO571.643e-2866.15Fumarate hydratase class II OS=Escherichia coli O1... [more]
FUMC_ECOL62.139e-2866.15Fumarate hydratase class II OS=Escherichia coli O6... [more]
FUMC_HELPY2.139e-2863.64Fumarate hydratase class II OS=Helicobacter pylori... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-002-A09-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY755965 ID=EY755965; Name=EY755965; organism=Citrus sinensis; type=EST; length=647bp
CAGTGGGTGGATCAAATGGCCATTTTGAACTAAATGTATTCAAGCCAATG
ATTGCTAGTGGTCTCCTACATTCACTGAGATTGCTGGGAGATGCATCTGC
TTCTTTTGAGAAGAACTGTGTGAGAGGAATTCAAGCTAATAGGGAAAGAT
TTCCAAATTGCTGCACGAGTCATTGATGCTTGTCACATCCTTGAATCCTA
AAATTGGTTATGACAATGCTGCGGCAGTTGCCAAGAAGGCTCACAAGGAG
GGAACTACACTTAAGGATGCTGCATTGAAACTCGGAGTACTCAATAGTGA
AGAATTTGATAATCTTGTGGTACCTGAGAAAATGATTGGCCCATCCGACT
AACCAGCTTATGTGGGTTAAACTCCTGGGATTTGCTTTTGGCTTTTGTAT
CATTTTGCGGGATATCTGTTTCTGCGCCGTTTCTCTCCCTCAAGGCCTCA
ACTGAATCACTTGATAGGGATCCAAATAGTGATTTTGCTTTTATTTAAAA
AAACAAAGAAAGGGGCAACCAAAAAAAAACTATATTCTTGAGGNTTTCCC
AGGAGGAGGCTTCGGTGATATGTCATTTCACGTGGTGACAGAATAGCGAT
TGGTAGGCCCACAAAGGAAGACCTCCCTCATTTTTTGTGCGACAGTG
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