EY755965

Overview
NameEY755965
Unique NameEY755965
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length647
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5506 contig Csv1_Contig5506:35..683. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_MYXXD (Fumarate hydratase class II OS=Myxococcus xanthus (strain DK 1622) GN=fumC PE=3 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 4.175e-26
Identity = 38/65 (58.46%), Postives = 49/65 (75.38%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I + L  SLMLVT+LNP IGYDNAA +AK AH++GTTLK+ A+ LG++  E+FD  V PE M G
Sbjct:  399 RIQENLERSLMLVTALNPHIGYDNAAKIAKTAHRDGTTLKETAVALGLVTPEQFDQWVRPEDMTG 463

HSP 2 Score: 59.6918 bits (143), Expect = 4.175e-26
Identity = 28/49 (57.14%), Postives = 35/49 (71.43%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            VGG++G+F+LNVFKP+IA  LL S RLL D   SF  +C  GI+ NR R
Sbjct:  351 VGGASGNFQLNVFKPLIAHNLLQSCRLLADGMRSFRLHCAVGIEPNRPR 399
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_BACCR (Fumarate hydratase class II OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=fumC PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 7.075e-26
Identity = 40/67 (59.70%), Postives = 54/67 (80.60%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGPSD 349
            I++ ++ SLMLVT+LNP IGY+NAA +AK AHKEG TLK+AAL+ G+L  E+FD +V P+KMI P +
Sbjct:  396 INENVNRSLMLVTALNPHIGYENAAKIAKHAHKEGLTLKEAALQSGLLTEEQFDEIVDPKKMIAPKE 462

HSP 2 Score: 53.5286 bits (127), Expect = 7.075e-26
Identity = 26/45 (57.78%), Postives = 30/45 (66.67%), Query Frame = 3 
Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            S G+FELNVFKP+IA   L S  LL DA  SF  NC  GI+A+ E
Sbjct:  350 SQGNFELNVFKPVIAYNFLQSAHLLADAIVSFNDNCAVGIEADEE 394
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_CHRVO (Fumarate hydratase class II OS=Chromobacterium violaceum GN=fumC PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 7.075e-26
Identity = 39/64 (60.94%), Postives = 52/64 (81.25%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMI 337
            +I++L+  SLMLVT+LNP IGYD AAA+AK AH+ GTTL++AAL LG L++E+FD  V PE M+
Sbjct:  398 RIAELMARSLMLVTALNPHIGYDKAAAIAKHAHRHGTTLREAALALGHLSAEQFDAWVRPEDMV 461

HSP 2 Score: 57.7658 bits (138), Expect = 7.075e-26
Identity = 26/49 (53.06%), Postives = 35/49 (71.43%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +G ++G+FELNVFKP+IA   L S RLL D   S  ++CV G++ANR R
Sbjct:  350 IGAASGNFELNVFKPLIAHNFLQSARLLADGMDSLREHCVDGMEANRAR 398
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_BORPE (Fumarate hydratase class II OS=Bordetella pertussis GN=fumC PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.198e-25
Identity = 37/63 (58.73%), Postives = 46/63 (73.02%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKM 334
            +I++L+  SLMLVT+LNP IGYD AA +AKKAHKE  +LK+AAL LG L   +F   VVP  M
Sbjct:  396 RITELVERSLMLVTALNPHIGYDKAAQIAKKAHKENLSLKEAALALGHLTEAQFAEWVVPGDM 458

HSP 2 Score: 64.6994 bits (156), Expect = 1.198e-25
Identity = 28/49 (57.14%), Postives = 37/49 (75.51%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            VGG++G+FELNVFKP++    L S+RLL D   SF+K+C  GI+ NRER
Sbjct:  348 VGGASGNFELNVFKPLVIHNFLQSVRLLADGMVSFDKHCAAGIEPNRER 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_BORPA (Fumarate hydratase class II OS=Bordetella parapertussis GN=fumC PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.198e-25
Identity = 37/63 (58.73%), Postives = 46/63 (73.02%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKM 334
            +I++L+  SLMLVT+LNP IGYD AA +AKKAHKE  +LK+AAL LG L   +F   VVP  M
Sbjct:  396 RITELVERSLMLVTALNPHIGYDKAAQIAKKAHKENLSLKEAALALGHLTEAQFAEWVVPGDM 458

HSP 2 Score: 64.6994 bits (156), Expect = 1.198e-25
Identity = 28/49 (57.14%), Postives = 37/49 (75.51%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            VGG++G+FELNVFKP++    L S+RLL D   SF+K+C  GI+ NRER
Sbjct:  348 VGGASGNFELNVFKPLVIHNFLQSVRLLADGMVSFDKHCAAGIEPNRER 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_BORBR (Fumarate hydratase class II OS=Bordetella bronchiseptica GN=fumC PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.198e-25
Identity = 37/63 (58.73%), Postives = 46/63 (73.02%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKM 334
            +I++L+  SLMLVT+LNP IGYD AA +AKKAHKE  +LK+AAL LG L   +F   VVP  M
Sbjct:  396 RITELVERSLMLVTALNPHIGYDKAAQIAKKAHKENLSLKEAALALGHLTEAQFAEWVVPGDM 458

HSP 2 Score: 64.6994 bits (156), Expect = 1.198e-25
Identity = 28/49 (57.14%), Postives = 37/49 (75.51%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            VGG++G+FELNVFKP++    L S+RLL D   SF+K+C  GI+ NRER
Sbjct:  348 VGGASGNFELNVFKPLVIHNFLQSVRLLADGMVSFDKHCAAGIEPNRER 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_BACAN (Fumarate hydratase class II OS=Bacillus anthracis GN=fumC PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.439e-25
Identity = 39/67 (58.21%), Postives = 53/67 (79.10%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGPSD 349
            I + ++ SLMLVT+LNP IGY+NAA +AK AHKEG TLK+AAL+ G+L  E+F+ +V P+KMI P +
Sbjct:  396 IKENVNRSLMLVTALNPHIGYENAAKIAKHAHKEGLTLKEAALQSGLLTEEQFNEIVDPKKMIAPKE 462

HSP 2 Score: 53.5286 bits (127), Expect = 3.439e-25
Identity = 26/45 (57.78%), Postives = 30/45 (66.67%), Query Frame = 3 
Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            S G+FELNVFKP+IA   L S  LL DA  SF  NC  GI+A+ E
Sbjct:  350 SQGNFELNVFKPVIAYNFLQSAHLLADAIVSFNDNCAVGIEADEE 394
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_PASMU (Fumarate hydratase class II OS=Pasteurella multocida GN=fumC PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.824e-25
Identity = 38/65 (58.46%), Postives = 50/65 (76.92%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I K L +SLMLVT+LN  IGY+NAA +AK AHK GTTLK+ A+ LG++ +E+FD  V PE M+G
Sbjct:  397 RIKKQLDDSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQFDEWVRPEDMVG 461

HSP 2 Score: 55.0694 bits (131), Expect = 5.824e-25
Identity = 24/47 (51.06%), Postives = 34/47 (72.34%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            G+ GHF+LNVFKP++A+  L S +LL DA  SF+++C  GI+ N  R
Sbjct:  351 GTQGHFQLNVFKPVMAANFLQSAQLLADACVSFDEHCAVGIEPNFPR 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_RHIME (Fumarate hydratase class II OS=Rhizobium meliloti GN=fumC PE=3 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 5.824e-25
Identity = 40/66 (60.61%), Postives = 48/66 (72.73%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGPS 346
            I   L  SLMLVT+L PKIGYDNAA +AK AHK GTTL++ A+  G +  EEFD +V PE MIGP+
Sbjct:  398 IKAALDRSLMLVTALAPKIGYDNAAKIAKTAHKNGTTLREEAVGGGYVTDEEFDAVVRPETMIGPA 463

HSP 2 Score: 56.225 bits (134), Expect = 5.824e-25
Identity = 25/46 (54.35%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            GS GHFELNV+ P++A   L S++LL DA+ SF  NCV GI+A  +
Sbjct:  351 GSQGHFELNVYNPLMAYNFLQSVQLLADAAISFTDNCVVGIEARED 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_COXBU (Fumarate hydratase class II OS=Coxiella burnetii GN=fumC PE=3 SV=2)

HSP 1 Score: 80.8777 bits (198), Expect = 5.825e-25
Identity = 40/64 (62.50%), Postives = 51/64 (79.69%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMI 337
            KI+  L+ SLMLVT+LN  IGYD A+ +AKKA+KEGTTLK+AAL+LG L + EFD  V P+KM+
Sbjct:  394 KINDYLNNSLMLVTALNQIIGYDKASEIAKKAYKEGTTLKEAALQLGYLTASEFDKAVDPKKMV 457

HSP 2 Score: 54.6842 bits (130), Expect = 5.825e-25
Identity = 24/49 (48.98%), Postives = 35/49 (71.43%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            + GS G+FELNV+KP++A  L+ S+ LL DA  SF  +C  GI+ N+E+
Sbjct:  346 IAGSQGNFELNVYKPVMAYNLIQSIYLLSDACRSFNDHCAVGIKPNQEK 394
The following BLAST results are available for this feature:
BLAST of EY755965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 97
Match NameE-valueIdentityDescription
FUMC_MYXXD4.175e-2658.46Fumarate hydratase class II OS=Myxococcus xanthus ... [more]
FUMC_BACCR7.075e-2659.70Fumarate hydratase class II OS=Bacillus cereus (st... [more]
FUMC_CHRVO7.075e-2660.94Fumarate hydratase class II OS=Chromobacterium vio... [more]
FUMC_BORPE1.198e-2558.73Fumarate hydratase class II OS=Bordetella pertussi... [more]
FUMC_BORPA1.198e-2558.73Fumarate hydratase class II OS=Bordetella parapert... [more]
FUMC_BORBR1.198e-2558.73Fumarate hydratase class II OS=Bordetella bronchis... [more]
FUMC_BACAN3.439e-2558.21Fumarate hydratase class II OS=Bacillus anthracis ... [more]
FUMC_PASMU5.824e-2558.46Fumarate hydratase class II OS=Pasteurella multoci... [more]
FUMC_RHIME5.824e-2560.61Fumarate hydratase class II OS=Rhizobium meliloti ... [more]
FUMC_COXBU5.825e-2562.50Fumarate hydratase class II OS=Coxiella burnetii G... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-002-A09-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY755965 ID=EY755965; Name=EY755965; organism=Citrus sinensis; type=EST; length=647bp
CAGTGGGTGGATCAAATGGCCATTTTGAACTAAATGTATTCAAGCCAATG
ATTGCTAGTGGTCTCCTACATTCACTGAGATTGCTGGGAGATGCATCTGC
TTCTTTTGAGAAGAACTGTGTGAGAGGAATTCAAGCTAATAGGGAAAGAT
TTCCAAATTGCTGCACGAGTCATTGATGCTTGTCACATCCTTGAATCCTA
AAATTGGTTATGACAATGCTGCGGCAGTTGCCAAGAAGGCTCACAAGGAG
GGAACTACACTTAAGGATGCTGCATTGAAACTCGGAGTACTCAATAGTGA
AGAATTTGATAATCTTGTGGTACCTGAGAAAATGATTGGCCCATCCGACT
AACCAGCTTATGTGGGTTAAACTCCTGGGATTTGCTTTTGGCTTTTGTAT
CATTTTGCGGGATATCTGTTTCTGCGCCGTTTCTCTCCCTCAAGGCCTCA
ACTGAATCACTTGATAGGGATCCAAATAGTGATTTTGCTTTTATTTAAAA
AAACAAAGAAAGGGGCAACCAAAAAAAAACTATATTCTTGAGGNTTTCCC
AGGAGGAGGCTTCGGTGATATGTCATTTCACGTGGTGACAGAATAGCGAT
TGGTAGGCCCACAAAGGAAGACCTCCCTCATTTTTTGTGCGACAGTG
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