EY755965

Overview
NameEY755965
Unique NameEY755965
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length647
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5506 contig Csv1_Contig5506:35..683. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_AGRT5 (Fumarate hydratase class II OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970) GN=fumC PE=3 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 9.864e-25
Identity = 38/65 (58.46%), Postives = 46/65 (70.77%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I K +  SLMLVT+LN K+GYD  A +AK AHK GTTL++ A+  G L +EEFD  V PE MIGP
Sbjct:  398 IRKGVENSLMLVTALNGKLGYDICAKIAKTAHKNGTTLREEAVGGGYLTNEEFDQYVRPENMIGP 462

HSP 2 Score: 60.077 bits (144), Expect = 9.864e-25
Identity = 27/46 (58.70%), Postives = 34/46 (73.91%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            GS GHFELNV+ PM+A   L S++LLGDA+ SF  NCV GI+A  +
Sbjct:  351 GSQGHFELNVYNPMMAYNFLQSVQLLGDAAVSFTDNCVVGIEARED 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_NEIMA (Fumarate hydratase class II OS=Neisseria meningitidis serogroup A GN=fumC PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.174e-24
Identity = 39/66 (59.09%), Postives = 49/66 (74.24%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            KI   LH SLMLVT+LN KIGY+NAA VAK A+K   +L++ A++LG+L  EEFD LVVP  M+ P
Sbjct:  396 KIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFDELVVPADMVHP 461

HSP 2 Score: 53.9138 bits (128), Expect = 2.174e-24
Identity = 25/49 (51.02%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            + G++G+FELNV+ P+IA  LL S+RLLGDA  SF ++C  GI+   E+
Sbjct:  348 MAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEPVPEK 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_SYNY3 (Fumarate hydratase class II OS=Synechocystis sp. (strain PCC 6803) GN=fumC PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.829e-24
Identity = 40/66 (60.61%), Postives = 47/66 (71.21%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            K+   L  SLMLVT+LNP IGYDNAA VAK A  +G TLK AA+ LG+L   +FD  VVPE+MI P
Sbjct:  398 KVKDFLDTSLMLVTALNPHIGYDNAALVAKTAFAQGITLKQAAVDLGLLTPAQFDAWVVPEQMIAP 463

HSP 2 Score: 55.0694 bits (131), Expect = 2.829e-24
Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = 3 
Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            S G+FELNVFKP+I    LHSL LL DA ASF ++ V G+Q N  +
Sbjct:  353 SQGNFELNVFKPVIIHNFLHSLHLLSDACASFRQHLVVGLQVNESK 398
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_HAEDU (Fumarate hydratase class II OS=Haemophilus ducreyi GN=fumC PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.829e-24
Identity = 36/65 (55.38%), Postives = 49/65 (75.38%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I + L  SLMLVT+LN  IGY+NAA +AK AHK GTTLK+ A+ LG++ +E+FD  V P+ M+G
Sbjct:  397 RIQQQLENSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQFDQWVRPQNMVG 461

HSP 2 Score: 55.4546 bits (132), Expect = 2.829e-24
Identity = 24/47 (51.06%), Postives = 33/47 (70.21%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            GS GHF+LNVFKP++A+  L S +LL D   SF+++C  GI+ N  R
Sbjct:  351 GSQGHFQLNVFKPVMAANFLQSAQLLADVCISFDEHCASGIEPNYPR 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_THEEB (Fumarate hydratase class II OS=Thermosynechococcus elongatus (strain BP-1) GN=fumC PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.681e-24
Identity = 40/65 (61.54%), Postives = 49/65 (75.38%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I   +  SLMLVT+LNP IGYD AAAVAKKA+ EG TLK+AA++LG L +EEFD  V  E M+G
Sbjct:  398 QIQAYVERSLMLVTALNPHIGYDKAAAVAKKAYSEGKTLKEAAVELGYLTAEEFDRWVRLELMLG 462

HSP 2 Score: 55.0694 bits (131), Expect = 3.681e-24
Identity = 23/49 (46.94%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            + GS G+FELNV+KP+I   +L S+ LL DA+ SF  +C+ G++ NR++
Sbjct:  350 IAGSQGNFELNVYKPLIIYNVLQSIALLSDAAQSFTDHCLVGVEPNRQQ 398
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_WIGBR (Fumarate hydratase class II OS=Wigglesworthia glossinidia brevipalpis GN=fumC PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.682e-24
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I K L ESLMLVTSL P IGYD +A +AK AH E  TLK+A LKL  ++  +FD LV PE MIG
Sbjct:  397 RIKKFLKESLMLVTSLTPHIGYDKSAKIAKLAHNENITLKEACLKLNYISESQFDILVRPENMIG 461

HSP 2 Score: 55.0694 bits (131), Expect = 3.682e-24
Identity = 25/49 (51.02%), Postives = 34/49 (69.39%), Query Frame = 3 
Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            +GG +G+F+LNVF+PMI    L S+R+L D   SF K CV GI+ N +R
Sbjct:  349 IGGLSGNFQLNVFRPMIIYNFLQSVRILSDGIKSFNKKCVIGIKPNIKR 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_HAEIN (Fumarate hydratase class II OS=Haemophilus influenzae GN=fumC PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.682e-24
Identity = 36/65 (55.38%), Postives = 50/65 (76.92%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIG 340
            +I + L  SLMLVT+LN  IGY+NAA +AK AHK GTTL++ A+ LG++++E+FD  V PE M+G
Sbjct:  397 RIKQQLENSLMLVTALNTHIGYENAAKIAKTAHKNGTTLREEAINLGLVSAEDFDKWVRPEDMVG 461

HSP 2 Score: 55.0694 bits (131), Expect = 3.682e-24
Identity = 24/47 (51.06%), Postives = 33/47 (70.21%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            GS GHF+LNVF P++ +  L S +LLGDA  SF+++C  GI+ N  R
Sbjct:  351 GSQGHFQLNVFNPVMIANFLQSAQLLGDACVSFDEHCAVGIEPNYPR 397
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_CAMJE (Fumarate hydratase class II OS=Campylobacter jejuni GN=fumC PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.791e-24
Identity = 39/66 (59.09%), Postives = 52/66 (78.79%), Query Frame = 2 
Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            KI   LH SLMLVT+LNP IGY+NAA VAK AHK+G +LK++ ++LG+++ E+F+  V P KMIGP
Sbjct:  396 KIDHNLHNSLMLVTALNPHIGYENAAKVAKNAHKKGISLKESTMELGLVSEEDFNKFVDPTKMIGP 461

HSP 2 Score: 48.9062 bits (115), Expect = 4.791e-24
Identity = 24/46 (52.17%), Postives = 30/46 (65.22%), Query Frame = 3 
Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            S G+FELNVFKP+I    L SL LL D+  SF  +C  GI+ NR +
Sbjct:  351 SQGNFELNVFKPVIIYNFLQSLDLLADSMHSFNIHCAVGIEPNRAK 396
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_BACSU (Fumarate hydratase class II OS=Bacillus subtilis GN=fumC PE=3 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.788e-23
Identity = 40/65 (61.54%), Postives = 50/65 (76.92%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I + L  SLMLVT+LNP IGY+NAA +AK AHKEG TLK+AALKL +L  E+F+ +V PE M+ P
Sbjct:  396 IQENLSNSLMLVTALNPHIGYENAAKIAKLAHKEGLTLKEAALKLELLTEEQFNEMVKPEDMVKP 460

HSP 2 Score: 49.2914 bits (116), Expect = 1.788e-23
Identity = 23/45 (51.11%), Postives = 29/45 (64.44%), Query Frame = 3 
Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            S G+FELNVFKP+I    L S++LL D   SF   C  GI+ N+E
Sbjct:  350 SQGNFELNVFKPVIIYNFLQSVQLLSDGMNSFHDKCAVGIEPNKE 394
[Close]
BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_RHILO (Fumarate hydratase class II OS=Rhizobium loti GN=fumC PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.326e-23
Identity = 36/65 (55.38%), Postives = 45/65 (69.23%), Query Frame = 2 
Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L  SLMLVT+L P IGYDNAA +AK AHK GTTL++ AL  G+++  ++D LV PE M  P
Sbjct:  400 IKAALDRSLMLVTALAPTIGYDNAAKIAKTAHKNGTTLREEALATGLVSEVDYDRLVRPEDMTHP 464

HSP 2 Score: 57.7658 bits (138), Expect = 2.326e-23
Identity = 26/46 (56.52%), Postives = 33/46 (71.74%), Query Frame = 3 
Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            GS GHFELNV+ P++A   L S++LL DAS SF  NCV GI+A  +
Sbjct:  353 GSQGHFELNVYNPLMAYNFLQSVQLLSDASVSFTDNCVVGIEARED 398
The following BLAST results are available for this feature:
BLAST of EY755965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 97
Match NameE-valueIdentityDescription
FUMC_AGRT59.864e-2558.46Fumarate hydratase class II OS=Agrobacterium tumef... [more]
FUMC_NEIMA2.174e-2459.09Fumarate hydratase class II OS=Neisseria meningiti... [more]
FUMC_SYNY32.829e-2460.61Fumarate hydratase class II OS=Synechocystis sp. (... [more]
FUMC_HAEDU2.829e-2455.38Fumarate hydratase class II OS=Haemophilus ducreyi... [more]
FUMC_THEEB3.681e-2461.54Fumarate hydratase class II OS=Thermosynechococcus... [more]
FUMC_WIGBR3.682e-2460.00Fumarate hydratase class II OS=Wigglesworthia glos... [more]
FUMC_HAEIN3.682e-2455.38Fumarate hydratase class II OS=Haemophilus influen... [more]
FUMC_CAMJE4.791e-2459.09Fumarate hydratase class II OS=Campylobacter jejun... [more]
FUMC_BACSU1.788e-2361.54Fumarate hydratase class II OS=Bacillus subtilis G... [more]
FUMC_RHILO2.326e-2355.38Fumarate hydratase class II OS=Rhizobium loti GN=f... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-002-A09-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY755965 ID=EY755965; Name=EY755965; organism=Citrus sinensis; type=EST; length=647bp
CAGTGGGTGGATCAAATGGCCATTTTGAACTAAATGTATTCAAGCCAATG
ATTGCTAGTGGTCTCCTACATTCACTGAGATTGCTGGGAGATGCATCTGC
TTCTTTTGAGAAGAACTGTGTGAGAGGAATTCAAGCTAATAGGGAAAGAT
TTCCAAATTGCTGCACGAGTCATTGATGCTTGTCACATCCTTGAATCCTA
AAATTGGTTATGACAATGCTGCGGCAGTTGCCAAGAAGGCTCACAAGGAG
GGAACTACACTTAAGGATGCTGCATTGAAACTCGGAGTACTCAATAGTGA
AGAATTTGATAATCTTGTGGTACCTGAGAAAATGATTGGCCCATCCGACT
AACCAGCTTATGTGGGTTAAACTCCTGGGATTTGCTTTTGGCTTTTGTAT
CATTTTGCGGGATATCTGTTTCTGCGCCGTTTCTCTCCCTCAAGGCCTCA
ACTGAATCACTTGATAGGGATCCAAATAGTGATTTTGCTTTTATTTAAAA
AAACAAAGAAAGGGGCAACCAAAAAAAAACTATATTCTTGAGGNTTTCCC
AGGAGGAGGCTTCGGTGATATGTCATTTCACGTGGTGACAGAATAGCGAT
TGGTAGGCCCACAAAGGAAGACCTCCCTCATTTTTTGTGCGACAGTG
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