EY755965 | Citrus Genome Database

Overview


Name	EY755965
Unique Name	EY755965
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	647

Libraries


Library Name	Type
Sweet orange leaf, field plant B	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig5506	contig	Csv1_Contig5506:35..683.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_PSESM (Fumarate hydratase class II OS=Pseudomonas syringae pv. tomato GN=fumC-1 PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 7.122e-22
Identity = 37/64 (57.81%), Postives = 48/64 (75.00%), Query Frame = 2

Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMI 337
            K+++ L   LMLVT+LNP IGYD +A +AKKA+ EG TL++AAL LG L  EEFD  V P+KM+
Sbjct:  395 KMAEHLERGLMLVTALNPHIGYDKSAHIAKKAYTEGLTLREAALALGYLTDEEFDAWVRPDKML 458

HSP 2 Score: 50.0618 bits (118), Expect = 7.122e-22
Identity = 20/46 (43.48%), Postives = 33/46 (71.74%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            S GH +LNV+KP+I   +L S+RLL D  ++F ++CV G++ + E+
Sbjct:  350 SQGHLQLNVYKPVIIHNVLQSIRLLADGCSNFNEHCVAGMEPDAEK 395

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAAW (Fumarate hydratase class II OS=Staphylococcus aureus (strain MW2) GN=fumC PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.205e-21
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA +AKKAHKEG TLK++A++ G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDP 460

HSP 2 Score: 46.9802 bits (110), Expect = 1.205e-21
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  350 SQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAAS (Fumarate hydratase class II OS=Staphylococcus aureus (strain MSSA476) GN=fumC PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.205e-21
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA +AKKAHKEG TLK++A++ G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDP 460

HSP 2 Score: 46.9802 bits (110), Expect = 1.205e-21
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  350 SQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAAR (Fumarate hydratase class II OS=Staphylococcus aureus (strain MRSA252) GN=fumC PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.205e-21
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA +AKKAHKEG TLK++A++ G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDP 460

HSP 2 Score: 46.9802 bits (110), Expect = 1.205e-21
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  350 SQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAAN (Fumarate hydratase class II OS=Staphylococcus aureus (strain N315) GN=fumC PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.205e-21
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA +AKKAHKEG TLK++A++ G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDP 460

HSP 2 Score: 46.9802 bits (110), Expect = 1.205e-21
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  350 SQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAAM (Fumarate hydratase class II OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=fumC PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.205e-21
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA +AKKAHKEG TLK++A++ G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDP 460

HSP 2 Score: 46.9802 bits (110), Expect = 1.205e-21
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  350 SQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAAC (Fumarate hydratase class II OS=Staphylococcus aureus (strain COL) GN=fumC PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.205e-21
Identity = 35/65 (53.85%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA +AKKAHKEG TLK++A++ G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDP 460

HSP 2 Score: 46.9802 bits (110), Expect = 1.205e-21
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:   12 SNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  350 SQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMH_SCHPO (Fumarate hydratase, mitochondrial OS=Schizosaccharomyces pombe GN=fum1 PE=2 SV=3)

HSP 1 Score: 63.1586 bits (152), Expect = 1.565e-21
Identity = 34/63 (53.97%), Postives = 42/63 (66.67%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMI 337
            I++ L +SLMLVT+LNP IGYDN A +AK A K  +TLK   + LG    E+FD  V PE MI
Sbjct:  453 IARHLRDSLMLVTALNPHIGYDNCAKIAKTALKNKSTLKHEFVTLGFGTPEQFDEWVRPELMI 515

HSP 2 Score: 60.8474 bits (146), Expect = 1.565e-21
Identity = 28/48 (58.33%), Postives = 36/48 (75.00%), Query Frame = 3

Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRE 146
            V G++GH ELNVFKP++A  +L S+RLLGDA  SF  +CV GI+ N E
Sbjct:  404 VAGASGHCELNVFKPLLAKNILSSIRLLGDACESFTDHCVVGIEPNYE 451

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_STAS1 (Fumarate hydratase class II OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=fumC PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.452e-21
Identity = 34/65 (52.31%), Postives = 49/65 (75.38%), Query Frame = 2

Query:  149 ISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGP 343
            I   L++SLMLVT+LNP IGY+ AA++AKKAH+EG TLK++A+  G +  E+F+  + PE M+ P
Sbjct:  396 IDNYLNQSLMLVTALNPHIGYEKAASIAKKAHREGLTLKESAIDSGYVTEEQFEQWIKPEDMVEP 460

HSP 2 Score: 46.595 bits (109), Expect = 3.452e-21
Identity = 22/52 (42.31%), Postives = 29/52 (55.77%), Query Frame = 3

Query:    3 VGGSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRERFPN 158
            +  S G+FELNV+KP+I    L S+ LL D   +F  NC  GI+   E   N
Sbjct:  347 IASSQGNFELNVYKPVILLNTLQSIYLLADGMDTFNNNCAVGIEPIPENIDN 398

BLAST of EY755965 vs. ExPASy Swiss-Prot
Match: FUMC_THET2 (Fumarate hydratase class II OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=fumC PE=3 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 9.882e-21
Identity = 35/68 (51.47%), Postives = 46/68 (67.65%), Query Frame = 2

Query:  146 KISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGTTLKDAALKLGVLNSEEFDNLVVPEKMIGPSD 349
            +I + L ++ ML T+LN  IGYD AA + KKA KE  TLK AAL+LG L  EEFD +VVP ++  P +
Sbjct:  397 RIEEHLQKNPMLATALNKAIGYDKAAEIVKKALKEKKTLKQAALELGYLTEEEFDRIVVPMRLAKPHE 464

HSP 2 Score: 52.373 bits (124), Expect = 9.882e-21
Identity = 24/47 (51.06%), Postives = 34/47 (72.34%), Query Frame = 3

Query:    9 GSNGHFELNVFKPMIASGLLHSLRLLGDASASFEKNCVRGIQANRER 149
            GS G+F+LNV+KP++A   L S+ LL DA ASF+ +  +GI+ N ER
Sbjct:  351 GSQGNFQLNVYKPVMAYSTLESINLLADAVASFDAHLAQGIEPNLER 397

The following BLAST results are available for this feature:

BLAST of EY755965 vs. ExPASy Swiss-Prot

BLAST of EY755965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 97


Match Name	E-value	Identity	Description
FUMC_PSESM	7.122e-22	57.81	Fumarate hydratase class II OS=Pseudomonas syringa...	[more]
FUMC_STAAW	1.205e-21	53.85	Fumarate hydratase class II OS=Staphylococcus aure...	[more]
FUMC_STAAS	1.205e-21	53.85	Fumarate hydratase class II OS=Staphylococcus aure...	[more]
FUMC_STAAR	1.205e-21	53.85	Fumarate hydratase class II OS=Staphylococcus aure...	[more]
FUMC_STAAN	1.205e-21	53.85	Fumarate hydratase class II OS=Staphylococcus aure...	[more]
FUMC_STAAM	1.205e-21	53.85	Fumarate hydratase class II OS=Staphylococcus aure...	[more]
FUMC_STAAC	1.205e-21	53.85	Fumarate hydratase class II OS=Staphylococcus aure...	[more]
FUMH_SCHPO	1.565e-21	53.97	Fumarate hydratase, mitochondrial OS=Schizosacchar...	[more]
FUMC_STAS1	3.452e-21	52.31	Fumarate hydratase class II OS=Staphylococcus sapr...	[more]
FUMC_THET2	9.882e-21	51.47	Fumarate hydratase class II OS=Thermus thermophilu...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS13-C1-001-002-A09-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY755965 ID=EY755965; Name=EY755965; organism=Citrus sinensis; type=EST; length=647bp
CAGTGGGTGGATCAAATGGCCATTTTGAACTAAATGTATTCAAGCCAATG
ATTGCTAGTGGTCTCCTACATTCACTGAGATTGCTGGGAGATGCATCTGC
TTCTTTTGAGAAGAACTGTGTGAGAGGAATTCAAGCTAATAGGGAAAGAT
TTCCAAATTGCTGCACGAGTCATTGATGCTTGTCACATCCTTGAATCCTA
AAATTGGTTATGACAATGCTGCGGCAGTTGCCAAGAAGGCTCACAAGGAG
GGAACTACACTTAAGGATGCTGCATTGAAACTCGGAGTACTCAATAGTGA
AGAATTTGATAATCTTGTGGTACCTGAGAAAATGATTGGCCCATCCGACT
AACCAGCTTATGTGGGTTAAACTCCTGGGATTTGCTTTTGGCTTTTGTAT
CATTTTGCGGGATATCTGTTTCTGCGCCGTTTCTCTCCCTCAAGGCCTCA
ACTGAATCACTTGATAGGGATCCAAATAGTGATTTTGCTTTTATTTAAAA
AAACAAAGAAAGGGGCAACCAAAAAAAAACTATATTCTTGAGGNTTTCCC
AGGAGGAGGCTTCGGTGATATGTCATTTCACGTGGTGACAGAATAGCGAT
TGGTAGGCCCACAAAGGAAGACCTCCCTCATTTTTTGTGCGACAGTG