DR406094

Overview
NameDR406094
Unique NameDR406094
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length637
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_WHEAT (Ubiquitin-conjugating enzyme E2 2 OS=Triticum aestivum GN=UBC2 PE=1 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 9.487e-32
Identity = 64/129 (49.61%), Postives = 86/129 (66.67%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMY 627
            S  A KR+ ++ K LQ+DPPA  S  P  +++  W A I GP D+P+ GG F +++ F  DYP KPP V F +++FHPNI ++GSICLDIL+ QWSP   ++ +L SI SLL DPNP+ P   E A MY
Sbjct:    2 STPARKRLMRDFKRLQQDPPAGISGAPHDNNITLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMY 130          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_MEDSA (Ubiquitin-conjugating enzyme E2 2 OS=Medicago sativa GN=UBC2 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 9.487e-32
Identity = 64/129 (49.61%), Postives = 85/129 (65.89%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMY 627
            S  A KR+ ++ K LQ DPPA  S  P  +++  W A I GP D+P+ GG F +S+ F  DYP KPP V F +++FHPNI ++GSICLDIL+ QWSP   ++ +L SI SLL DPNP+ P   E A M+
Sbjct:    2 STPARKRLMRDFKRLQHDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMF 130          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBE2N_DICDI (Probable ubiquitin-conjugating enzyme E2 N OS=Dictyostelium discoideum GN=ube2n PE=3 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.239e-31
Identity = 66/127 (51.97%), Postives = 85/127 (66.93%), Query Frame = 1
Query:  256 KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            KRI KE + L  +P    SA P  D+  +++  I GP DSPF GGVF + +    DYP   PKV F TK++HPNI+  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL  ++A  +KT+
Sbjct:    6 KRIIKETQRLMTEPAPGISATPATDNFRYFKVAISGPLDSPFEGGVFNLELFLTDDYPMSAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEHWKTN 132          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_ASPFU (Ubiquitin-conjugating enzyme E2 2 OS=Aspergillus fumigatus GN=ubc2 PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 3.605e-31
Identity = 60/130 (46.15%), Postives = 85/130 (65.38%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +Q DPPA  SA PVAD++  W A I+GP+D+PF  G F + +HF   YP KPP V F +++FHPN+   G +CLDIL+ +WSP   ++ +L SI SLL DPN   P   E +++YK
Sbjct:    2 STSARRRLMRDFKRMQTDPPAGVSASPVADNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPGVKFISQMFHPNVYGTGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNTSSPANVEASNLYK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBCD6_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=UbcD6 PE=2 SV=2)

HSP 1 Score: 134.035 bits (336), Expect = 6.149e-31
Identity = 61/130 (46.92%), Postives = 84/130 (64.62%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K LQ+DPP   S  P  +++  W A I GP D+PF  G F ++I F  +YP KPP V F +KVFHPN+ ++G ICLDIL+ +WSP   +S +L SI SLL+DPNP+ P     A +YK
Sbjct:    2 STPARRRLMRDFKRLQEDPPTGVSGAPTDNNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKVFHPNVYADGGICLDILQNRWSPTYDVSAILTSIQSLLSDPNPNSPANSTAAQLYK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_EMENI (Ubiquitin-conjugating enzyme E2 2 OS=Emericella nidulans GN=uvsJ PE=3 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 8.031e-31
Identity = 59/130 (45.38%), Postives = 85/130 (65.38%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +Q DPPA  SA PVAD++  W A I+GP+D+PF  G F + +HF   YP KPP V F +++FHPN+   G +CLDIL+ +WSP   ++ +L SI SLL DPN   P   E +++Y+
Sbjct:    2 STSARRRLMRDFKRMQTDPPAGVSASPVADNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPGVKFISQMFHPNVYGTGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNTSSPANVEASNLYR 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC35_ARATH (Ubiquitin carrier protein E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.049e-30
Identity = 64/127 (50.39%), Postives = 86/127 (67.72%), Query Frame = 1
Query:  256 KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            +RI KE + L  +P    SA P  D+M ++   I+GP+ SP+ GGVF + +  P +YP   PKV F TK++HPNI+  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +K++
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSN 134          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_TRIHA (Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma harzianum GN=UBC2 PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.049e-30
Identity = 59/130 (45.38%), Postives = 86/130 (66.15%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +Q DPPA  SA P+ D++  W A I+GP+D+PF  G F + + F   YP KPP+V F +++FHPN+ +NG +CLDIL+ +WSP   ++ VL SI SLL DPN   P   E +++YK
Sbjct:    2 STAARRRLMRDFKRMQTDPPAGVSASPIPDNVMTWNAVIIGPADTPFEDGTFRLVMQFEEQYPNKPPQVKFISQMFHPNVYANGELCLDILQNRWSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC2_CANAL (Ubiquitin-conjugating enzyme E2 2 OS=Candida albicans GN=UBC2 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 1.370e-30
Identity = 58/130 (44.62%), Postives = 87/130 (66.92%), Query Frame = 1
Query:  241 SQMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK 630
            S  A +R+ ++ K +Q+DPP+  SA P+ D++  W A I+GPSD+PF  G F + + F   YP KPP+V F +++FHPN+ ++G +CLDIL+ +WSP   +S +L S+ SLL DPN   P   E A++YK
Sbjct:    2 STPARRRLMRDFKRMQQDPPSGVSASPLPDNVMKWNAVIIGPSDTPFEDGTFRLLLSFDEQYPNKPPQVKFISEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSVQSLLNDPNISSPANVEAANLYK 131          
BLAST of DR406094 vs. ExPASy Swiss-Prot
Match: UBC36_ARATH (Ubiquitin carrier protein E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 3.052e-30
Identity = 63/127 (49.61%), Postives = 86/127 (67.72%), Query Frame = 1
Query:  256 KRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPSDSPFAGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTD 636
            +RI KE + L  +P    SA P  ++M ++   I+GP+ SP+ GGVF + +  P +YP   PKV F TK++HPNI+  G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +K++
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSN 134          
The following BLAST results are available for this feature:
BLAST of DR406094 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 266
Match NameE-valueIdentityDescription
UBC2_WHEAT9.487e-3249.61Ubiquitin-conjugating enzyme E2 2 OS=Triticum aest... [more]
UBC2_MEDSA9.487e-3249.61Ubiquitin-conjugating enzyme E2 2 OS=Medicago sati... [more]
UBE2N_DICDI1.239e-3151.97Probable ubiquitin-conjugating enzyme E2 N OS=Dict... [more]
UBC2_ASPFU3.605e-3146.15Ubiquitin-conjugating enzyme E2 2 OS=Aspergillus f... [more]
UBCD6_DROME6.149e-3146.92Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi... [more]
UBC2_EMENI8.031e-3145.38Ubiquitin-conjugating enzyme E2 2 OS=Emericella ni... [more]
UBC35_ARATH1.049e-3050.39Ubiquitin carrier protein E2 35 OS=Arabidopsis tha... [more]
UBC2_TRIHA1.049e-3045.38Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma h... [more]
UBC2_CANAL1.370e-3044.62Ubiquitin-conjugating enzyme E2 2 OS=Candida albic... [more]
UBC36_ARATH3.052e-3049.61Ubiquitin carrier protein E2 36 OS=Arabidopsis tha... [more]

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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248L04 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248L04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR406094 ID=DR406094; Name=DR406094; organism=Citrus sinensis; type=EST; length=637bp
TTTTTTTTTTTAAATTTCNNAATTATTTCATTAATTTTTAAAATTCCCCA
ATAATCACGCCTTCGTCCCTGATTTTGGTTTGAATTTATAAATAAAGGTT
GTTGCTTCAAATCTGTCGGTACAATTTTTGTACTTTTTAGAATTAGGGTT
AGGGTTTGTGATCGTGTTTTCGATTCTGTTTGCTTTTAAGATATAACCCT
CGAAGTACGGCACTGTTTCTCTGATGGTTTGTTGAGAAATTCCCAGATGG
CTTCAAAACGGATCAACAAGGAGTTGAAGGACCTACAGAAGGACCCTCCT
GCTTCTTGTAGCGCTGGCCCTGTTGCTGATGACATGTTCCATTGGCAAGC
AACAATTATGGGCCCATCAGACAGCCCATTTGCTGGGGGTGTATTTCTTG
TATCCATTCATTTCCCACCTGATTATCCATTCAAGCCACCGAAGGTGGCC
TTCCGTACTAAGGTTTTCCACCCTAACATCAATAGCAATGGTAGCATCTG
TCTTGACATTCTCAAGGAGCAGTGGAGCCCTGCTTTGACAATATCCAAGG
TACTTCTGTCAATTTGCTCACTACTTACTGATCCGAACCCAGATGACCCA
CTTGTGCCTGAGATTGCTCACATGTACAAGACTGACA
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