DC900183

Overview
NameDC900183
Unique NameDC900183
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length672
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig8429 contig Csv1_Contig8429:927..1598. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RAP2A_PONAB (Ras-related protein Rap-2a OS=Pongo abelii GN=RAP2A PE=2 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.276e-13
Identity = 46/162 (28.40%), Postives = 89/162 (54.94%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRV--CENIPIVLCGNKVDVKN-RQVKAKQ-VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +K+V++G GG GK+    + +TG F +KY+PTI  + +  +   +         DTAG E+F  +RD Y  +GQ  I+++ +  + +++++      + RV   E +P++L GNKVD+++ R+V + +      +    + E SAKS    ++ F  + R++
Sbjct:    4 YKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTI-EDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSNEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQM 164          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RAP2A_MOUSE (Ras-related protein Rap-2a OS=Mus musculus GN=Rap2a PE=1 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.276e-13
Identity = 46/162 (28.40%), Postives = 89/162 (54.94%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRV--CENIPIVLCGNKVDVKN-RQVKAKQ-VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +K+V++G GG GK+    + +TG F +KY+PTI  + +  +   +         DTAG E+F  +RD Y  +GQ  I+++ +  + +++++      + RV   E +P++L GNKVD+++ R+V + +      +    + E SAKS    ++ F  + R++
Sbjct:    4 YKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTI-EDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSNEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQM 164          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RAP2A_HUMAN (Ras-related protein Rap-2a OS=Homo sapiens GN=RAP2A PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.276e-13
Identity = 46/162 (28.40%), Postives = 89/162 (54.94%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRV--CENIPIVLCGNKVDVKN-RQVKAKQ-VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +K+V++G GG GK+    + +TG F +KY+PTI  + +  +   +         DTAG E+F  +RD Y  +GQ  I+++ +  + +++++      + RV   E +P++L GNKVD+++ R+V + +      +    + E SAKS    ++ F  + R++
Sbjct:    4 YKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTI-EDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQM 164          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RAB17_MOUSE (Ras-related protein Rab-17 OS=Mus musculus GN=Rab17 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.276e-13
Identity = 53/185 (28.65%), Postives = 90/185 (48.65%), Query Frame = 2
Query:   38 QLMALPSQQTVDYPSF--KLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCE--NIPIVLCGNKVDV-KNRQVKAKQ-VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL---AGD 565
            Q   LP   T     +  KLV++G    GKT+   R++  +F     PT+G              ++   WDTAGQEK+  +   Y+     A++++D+T + ++     W  DL +  +   + ++L GNK D+ + R+V  ++   F   K+L + E SAK NY   + F  +A++L   AGD
Sbjct:    3 QAAGLPQASTASGQPYVSKLVLLGSSSVGKTSLALRYMKQDFSNVL-PTVGCAFFTKVLDLGSSSLKLEIWDTAGQEKYQSVCHLYFRGANAALLVYDITRKDSFHKAQQWLEDLEKEFQPGEVVVMLVGNKTDLGEEREVTFQEGKEFAESKSLLFMETSAKLNYQVSEIFNTVAQELLQRAGD 186          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RB22A_CANFA (Ras-related protein Rab-22A OS=Canis familiaris GN=RAB22A PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.667e-13
Identity = 48/160 (30.00%), Postives = 79/160 (49.38%), Query Frame = 2
Query:   86 KLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR-VCENIPIVLCGNKVD-VKNRQVKAKQVTFHRKK-NLQYYEISAKSNYNFEKPFLYLARKL 556
            K+ ++GD G GK++ V R +   F+    PTIG               +F  WDTAGQE F  L   YY     AII++D+T   T+  +  W ++L +    NI + + GNK D +  R+V  +    +    +  + E SAK+  N  + F+ ++R++
Sbjct:    7 KVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQEAFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVKELRQHGPPNIVVAIAGNKCDLIDVREVMERDAKDYADSIHAIFVETSAKNAININELFIEISRRI 166          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RAS_LENED (Ras-like protein OS=Lentinula edodes PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.667e-13
Identity = 51/172 (29.65%), Postives = 88/172 (51.16%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTI----------GVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRV--CENIPIVLCGNKVDVK-NRQVKAKQ-VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +KLV+VG GG GK+    + +   F  +Y+PTI            EV  LD             DTAGQE++G +R+ Y   G+  ++++ +T+R +++ + T+H+ + RV   +  P+V+  NK D++  RQV   +     R    ++ E SAK   N ++ F  L R++
Sbjct:   11 YKLVVVGGGGVGKSALTIQFIQSHFVDEYDPTIEDSYRKQCVIDDEVALLDVL-----------DTAGQEEYGAMREQYMRTGEGFLLVYSITSRNSFEEISTFHQQILRVKDQDTFPVVVVANKCDLEYERQVGMNEGRDLARHFGCKFVETSAKVRINVDQAFQDLVREI 171          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RASL_COLTR (Ras-like protein OS=Colletotrichum trifolii GN=RAS PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.667e-13
Identity = 48/172 (27.91%), Postives = 87/172 (50.58%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTI----------GVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCEN--IPIVLCGNKVDVKNRQVKAKQ--VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +KLV+VG GG GK+    + +   F  +Y+PTI            EV  LD             DTAGQE++  +R+ Y   G+  ++++ +T+R +++ + T+ + + RV +    P+V+ GNK D++  +   +Q      +    ++ E SAKS  N +K F  + R++
Sbjct:    9 YKLVVVGGGGVGKSCLTIQLIQSHFVDEYDPTIEDSYRKQCVIDEEVALLDVL-----------DTAGQEEYSAMREQYMRTGEGFLLVYSITSRQSFEEITTFQQQILRVKDKDYFPMVVVGNKCDLEGEREVTRQEGEALAKSFGCKFIETSAKSRINVDKAFYDIVREI 169          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RASH_RAT (GTPase HRas OS=Rattus norvegicus GN=Hras1 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.667e-13
Identity = 47/161 (29.19%), Postives = 83/161 (51.55%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRV--CENIPIVLCGNKVDVKNRQVKAKQV-TFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +KLV+VG GG GK+    + +   F  +Y+PTI  + +      +         DTAGQE++  +RD Y   G+  + +F +    +++++  +   + RV   +++P+VL GNK D+  R V+++Q     R   + Y E SAK+    E  F  L R++
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTI-EDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI 163          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RASH_HUMAN (GTPase HRas OS=Homo sapiens GN=HRAS PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.667e-13
Identity = 47/161 (29.19%), Postives = 83/161 (51.55%), Query Frame = 2
Query:   83 FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRV--CENIPIVLCGNKVDVKNRQVKAKQV-TFHRKKNLQYYEISAKSNYNFEKPFLYLARKL 556
            +KLV+VG GG GK+    + +   F  +Y+PTI  + +      +         DTAGQE++  +RD Y   G+  + +F +    +++++  +   + RV   +++P+VL GNK D+  R V+++Q     R   + Y E SAK+    E  F  L R++
Sbjct:    4 YKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTI-EDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI 163          
BLAST of DC900183 vs. ExPASy Swiss-Prot
Match: RASEF_MOUSE (RAS and EF-hand domain-containing protein homolog OS=Mus musculus GN=Rasef PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.667e-13
Identity = 48/169 (28.40%), Postives = 83/169 (49.11%), Query Frame = 2
Query:   80 SFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCR--VCENIPIVLCGNKVDVK--NRQVKAKQVTFHRKKNLQ------YYEISAKSNYNFEKPFLYLARKL 556
            ++K+V+ GD   GK++F+ R    EF+     T+GV+        +  +     WDTAGQE+F  +   Y+      ++++DVT   ++ NV  W  D+        IPI+L GNK D++  +     K ++ +  + L       + E SAK   N  +  L+LAR++
Sbjct:  428 AYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLIVDGEQTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREW-VDMVEDGTHRTIPIMLVGNKADLRDVDNAENQKCISAYLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREV 595          
The following BLAST results are available for this feature:
BLAST of DC900183 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RAP2A_PONAB1.276e-1328.40Ras-related protein Rap-2a OS=Pongo abelii GN=RAP2... [more]
RAP2A_MOUSE1.276e-1328.40Ras-related protein Rap-2a OS=Mus musculus GN=Rap2... [more]
RAP2A_HUMAN1.276e-1328.40Ras-related protein Rap-2a OS=Homo sapiens GN=RAP2... [more]
RAB17_MOUSE1.276e-1328.65Ras-related protein Rab-17 OS=Mus musculus GN=Rab1... [more]
RB22A_CANFA1.667e-1330.00Ras-related protein Rab-22A OS=Canis familiaris GN... [more]
RAS_LENED1.667e-1329.65Ras-like protein OS=Lentinula edodes PE=3 SV=1[more]
RASL_COLTR1.667e-1327.91Ras-like protein OS=Colletotrichum trifolii GN=RAS... [more]
RASH_RAT1.667e-1329.19GTPase HRas OS=Rattus norvegicus GN=Hras1 PE=1 SV=... [more]
RASH_HUMAN1.667e-1329.19GTPase HRas OS=Homo sapiens GN=HRAS PE=1 SV=1[more]
RASEF_MOUSE1.667e-1328.40RAS and EF-hand domain-containing protein homolog ... [more]

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Properties
Property NameValue
Genbank descriptionDC900183 VSS Citrus sinensis cDNA clone VS28210 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900183 ID=DC900183|Name=DC900183|organism=Citrus sinensis|type=EST|length=672bp
CAGACCGCGAACTTCGTATTCTTTCGCACACAGTACTCAACTCATGGCTC
TGCCAAGTCAGCAAACTGTTGATTACCCGAGCTTCAAACTCGTCATAGTC
GGCGACGGTGGCACTGGGAAGACTACCTTTGTGAAGAGGCATCTGACTGG
GGAGTTTGAGAAGAAGTATGAACCAACTATTGGTGTGGAGGTTCATCCGT
TGGATTTCTTTACAAATTGTGGCAAAATCCGTTTCTACTGCTGGGACACT
GCTGGCCAGGAAAAGTTTGGTGGTCTTAGAGATGGTTATTACATCCATGG
GCAATGTGCCATCATTATGTTTGATGTTACTGCCCGATTGACATACAAGA
ATGTTCCCACTTGGCACCGTGATCTTTGCCGGGTGTGTGAAAATATACCG
ATTGTTCTCTGTGGGAACAAGGTTGATGTGAAGAATAGGCAAGTCAAGGC
AAAGCAGGTTACCTTCCACAGGAAGAAGAATCTACAGTACTATGAGATTT
CTGCCAAGAGCAACTACAATTTTGAGAAACCTTTCTTGTACCTTGCCAGG
AAGCTTGCTGGGGACCCAAATTTGCACTTCGTGGAATCTCCTGCTCTCGC
TCCTCCAGAAGTACAGATTGACTTAGCTGACAGCAACACATGAAGCCGAG
CTTGCCGANCAGCTAGCCAGCC
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