DC900180

Overview
NameDC900180
Unique NameDC900180
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length596
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1582 contig Csv1_Contig1582:410..1005. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 14311_ARATH (14-3-3-like protein GF14 omicron OS=Arabidopsis thaliana GN=GRF11 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.404e-55
Identity = 109/136 (80.15%), Postives = 118/136 (86.76%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGE 409
            +HL+P +TSGESTVFYYKMKGDY+RYLAEFK   DR+EAAD SLK          +EL  THPIRLGLALNFSVFYYEILNSPERACHLAK+AFDEAIAELDSLNE+SYKDSTLIMQLLRDNLTLWTSDL EEGG+
Sbjct:  107 KHLVPFATSGESTVFYYKMKGDYFRYLAEFKSGADREEAADLSLKAYEAATSSASTELSTTHPIRLGLALNFSVFYYEILNSPERACHLAKRAFDEAIAELDSLNEDSYKDSTLIMQLLRDNLTLWTSDL-EEGGK 241
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433_CHLRE (14-3-3-like protein OS=Chlamydomonas reinhardtii PE=2 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.648e-54
Identity = 105/147 (71.43%), Postives = 121/147 (82.31%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPE-EGGEQSKGDEVQAE 439
            +HLIP++++GES VFY KMKGDY+RYLAEFK   DRKEAA+H+L            +LPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSL EESYKDSTLIMQLLRDNLTLWTSD+ +   G+  +G +++ E
Sbjct:  109 DHLIPTASTGESKVFYLKMKGDYHRYLAEFKTGADRKEAAEHTLLAYKAAQDIALVDLPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLGEESYKDSTLIMQLLRDNLTLWTSDMQDPAAGDDREGADMKVE 255
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433E_SHEEP (14-3-3 protein epsilon OS=Ovis aries GN=YWHAE PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 102/139 (73.38%), Postives = 118/139 (84.89%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSK 418
            +HLIP++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+  +G EQ+K
Sbjct:  106 KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNK 244
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433E_RAT (14-3-3 protein epsilon OS=Rattus norvegicus GN=Ywhae PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 102/139 (73.38%), Postives = 118/139 (84.89%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSK 418
            +HLIP++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+  +G EQ+K
Sbjct:  106 KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNK 244
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433E_MOUSE (14-3-3 protein epsilon OS=Mus musculus GN=Ywhae PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 102/139 (73.38%), Postives = 118/139 (84.89%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSK 418
            +HLIP++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+  +G EQ+K
Sbjct:  106 KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNK 244
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433E_HUMAN (14-3-3 protein epsilon OS=Homo sapiens GN=YWHAE PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 102/139 (73.38%), Postives = 118/139 (84.89%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSK 418
            +HLIP++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+  +G EQ+K
Sbjct:  106 KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNK 244
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433E_CHICK (14-3-3 protein epsilon OS=Gallus gallus GN=YWHAE PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 102/139 (73.38%), Postives = 118/139 (84.89%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSK 418
            +HLIP++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+  +G EQ+K
Sbjct:  106 KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNK 244
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433E_BOVIN (14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 102/139 (73.38%), Postives = 118/139 (84.89%), Query Frame = 2 
Query:    2 EHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSK 418
            +HLIP++ +GES VFYYKMKGDY+RYLAEF   +DRKEAA++SL           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAK AFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+  +G EQ+K
Sbjct:  106 KHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNK 244
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: 1433B_HORVU (14-3-3-like protein B OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.716e-53
Identity = 105/149 (70.47%), Postives = 121/149 (81.21%), Query Frame = 2 
Query:    5 HLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSKGDEVQAES*PG 451
            HL+PSST+ ES VFY KMKGDYYRYLAEFK   +RK+AA++++           +ELPPTHPIRLGLALNFSVFYYEILNSP+RAC LAKQAFDEAI+ELDSL+EESYKDSTLIMQLLRDNLTLWTSD+ E+  E+ K D  + ES  G
Sbjct:  114 HLVPSSTAPESKVFYLKMKGDYYRYLAEFKSGTERKDAAENTMVAYKAAQEIALAELPPTHPIRLGLALNFSVFYYEILNSPDRACDLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDISEDAAEEMK-DAPKGESGDG 261
[Close]
BLAST of DC900180 vs. ExPASy Swiss-Prot
Match: BMH2_YEAST (Protein BMH2 OS=Saccharomyces cerevisiae GN=BMH2 PE=1 SV=3)

HSP 1 Score: 208.379 bits (529), Expect = 2.241e-53
Identity = 101/145 (69.66%), Postives = 118/145 (81.38%), Query Frame = 2 
Query:    5 HLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLNEESYKDSTLIMQLLRDNLTLWTSDLPEEGGEQSKGDEVQAE 439
            HLIPS+T+GES VFYYKMKGDY+RYLAEF   D R++A + SL+          +ELPPTHPIRLGLALNFSVFYYEI NSP++ACHLAKQAFD+AIAELD+L+EESYKDSTLIMQLLRDNLTLWTSD+ E G E  +  + Q +
Sbjct:  109 HLIPSATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATTELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDISESGQEDQQQQQQQQQ 253
The following BLAST results are available for this feature:
BLAST of DC900180 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 143
Match NameE-valueIdentityDescription
14311_ARATH1.404e-5580.1514-3-3-like protein GF14 omicron OS=Arabidopsis th... [more]
1433_CHLRE2.648e-5471.4314-3-3-like protein OS=Chlamydomonas reinhardtii P... [more]
1433E_SHEEP1.716e-5373.3814-3-3 protein epsilon OS=Ovis aries GN=YWHAE PE=1... [more]
1433E_RAT1.716e-5373.3814-3-3 protein epsilon OS=Rattus norvegicus GN=Ywh... [more]
1433E_MOUSE1.716e-5373.3814-3-3 protein epsilon OS=Mus musculus GN=Ywhae PE... [more]
1433E_HUMAN1.716e-5373.3814-3-3 protein epsilon OS=Homo sapiens GN=YWHAE PE... [more]
1433E_CHICK1.716e-5373.3814-3-3 protein epsilon OS=Gallus gallus GN=YWHAE P... [more]
1433E_BOVIN1.716e-5373.3814-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2... [more]
1433B_HORVU1.716e-5370.4714-3-3-like protein B OS=Hordeum vulgare PE=2 SV=1[more]
BMH2_YEAST2.241e-5369.66Protein BMH2 OS=Saccharomyces cerevisiae GN=BMH2 P... [more]

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Properties
Property NameValue
Genbank descriptionDC900180 VSS Citrus sinensis cDNA clone VS28206 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900180 ID=DC900180; Name=DC900180; organism=Citrus sinensis; type=EST; length=596bp
TGAACACCTAATCCCATCCTCCACATCTGGAGAATCAACCGTGTTTTACT
ATAAGATGAAAGGAGATTATTACCGTTACCTAGCTGAGTTCAAAGGTGCT
GATGATCGTAAGGAAGCTGCAGATCATTCACTTAAGGCTTACGAGGCTGC
TACAGCTGCAGCAGCCTCAGAGTTACCCCCAACTCATCCAATCAGACTTG
GCCTGGCTCTTAACTTTTCTGTTTTCTACTATGAGATTTTGAACTCACCT
GAGAGGGCTTGCCACCTGGCTAAACAAGCATTTGATGAGGCTATTGCTGA
ACTTGATAGCCTTAATGAAGAGTCCTACAAGGACAGCACCCTTATTATGC
AGCTTCTTCGGGATAATCTCACCTTGTGGACCTCGGATCTACCGGAGGAG
GGAGGTGAGCAATCTAAAGGTGATGAAGTTCAAGCAGAGAGCTAGCCGGG
GATGCATGAGCATCAGGAGGAGAAATTGTGCTAGTTGGGTTCTAATATCT
ATTAGTATTAGTAGTATAGGGATTGAAAGAACATTTGGCTTGTATAATAC
TTATTACATGTTTTTAATTTCAGGTTGTTGTGCTATTTTCTGTTTA
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