DC900171

Overview
NameDC900171
Unique NameDC900171
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length656
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5386 contig Csv1_Contig5386:85..740. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FADH2_SCHPO (Putative S-(hydroxymethyl)glutathione dehydrogenase 2 OS=Schizosaccharomyces pombe GN=SPCC13B11.04c PE=2 SV=2)

HSP 1 Score: 204.142 bits (518), Expect = 5.129e-52
Identity = 103/199 (51.76%), Postives = 130/199 (65.33%), Query Frame = 3 
Query:    6 TAGQVIRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            TAG++I CKAAVAW+   PL IE V+V PP+ +EVR+KI+ + +CHTD Y    K    LFP I GHE  GIVESVG  VT +Q GD V+ ++T ECK C  C S ++N+C  +R    +G+M  DG SRFS  G  + HF+G STFSEYTV     V  I   APLD VC+L CGI+TG+GA    A  K+G SVA+F
Sbjct:    6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGKGLM-PDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCGITTGYGAATITADIKEGDSVAVF 203
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_SHISS (S-(hydroxymethyl)glutathione dehydrogenase OS=Shigella sonnei (strain Ss046) GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_PASPI (S-(hydroxymethyl)glutathione dehydrogenase OS=Pasteurella piscicida GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 97/194 (50.00%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  G PL I E++VAPP+K EV +KI  T +CHTD Y         LFP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +GVM  D  SRF + G+P+YH++G STFSEYTV     +AKINP A  ++VC+L CG++TG GA  N AK ++G SVA+F
Sbjct:    1 MKSRAAVAFGPGLPLEIVEIDVAPPKKGEVLVKISHTGVCHTDAYTLSGDDPEGLFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECDFCTSGKTNLCVAVRETQGKGVM-PDATSRFFVNGQPLYHYMGCSTFSEYTVVAEVSLAKINPQANAEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECOUT (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain UTI89 / UPEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLI (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain K12) GN=frmA PE=1 SV=3)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLC (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECOL5 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECOK1 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O1:K1 / APEC GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECOHS (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O9:H4 (strain HS) GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
[Close]
BLAST of DC900171 vs. ExPASy Swiss-Prot
Match: FRMA_ECO57 (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli O157:H7 GN=frmA PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 8.748e-52
Identity = 95/194 (48.97%), Postives = 132/194 (68.04%), Query Frame = 3 
Query:   21 IRCKAAVAWEAGKPLVIEEVEVAPPQKNEVRLKILFTSLCHTDVYFWEAKGQTPLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPVFTGECKECPHCHSEESNMCDLLRINTDRGVMINDGQSRFSIKGKPIYHFVGTSTFSEYTVTHVGCVAKINPAAPLDKVCVLSCGISTGFGATVNVAKPKKGQSVAIF 602
            ++ +AAVA+  GKPL I E++VAPP+K EV +K+  T +CHTD +         +FP + GHE  G+V  VGEGVT ++PGDHV+P++T EC EC  C S ++N+C  +R    +G+M  DG +RFS  G+P+YH++G STFSEYTV     +AKINP A  + VC+L CG++TG GA  N AK + G SVA+F
Sbjct:    1 MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLM-PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVF 193
The following BLAST results are available for this feature:
BLAST of DC900171 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 139
Match NameE-valueIdentityDescription
FADH2_SCHPO5.129e-5251.76Putative S-(hydroxymethyl)glutathione dehydrogenas... [more]
FRMA_SHISS8.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Shig... [more]
FRMA_PASPI8.748e-5250.00S-(hydroxymethyl)glutathione dehydrogenase OS=Past... [more]
FRMA_ECOUT8.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOLI8.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOLC8.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOL58.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOK18.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECOHS8.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]
FRMA_ECO578.748e-5248.97S-(hydroxymethyl)glutathione dehydrogenase OS=Esch... [more]

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Properties
Property NameValue
Genbank descriptionDC900171 VSS Citrus sinensis cDNA clone VS27V94 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900171 ID=DC900171; Name=DC900171; organism=Citrus sinensis; type=EST; length=656bp
CTGGCACAGCTGGTCAAGTTATTCGCTGCAAAGCGGCGGTGGCATGGGAG
GCGGGGAAGCCACTGGTGATTGAAGAAGTGGAGGTGGCTCCACCACAGAA
AAATGAAGTTCGATTGAAGATCCTCTTCACCTCTTTATGCCATACTGATG
TTTACTTCTGGGAAGCCAAGGGACAAACACCTCTCTTTCCTCGCATTTTT
GGTCACGAAGCTGGAGGGATTGTAGAAAGTGTAGGGGAGGGGGTAACTGA
TCTCCAACCAGGAGACCATGTTCTTCCTGTGTTCACAGGAGAGTGCAAGG
AATGTCCTCACTGTCATTCAGAGGAAAGCAATATGTGTGACCTCCTCAGG
ATCAATACTGACAGGGGTGTTATGATTAATGATGGTCAGTCGAGATTCTC
GATTAAGGGGAAGCCAATTTACCATTTTGTGGGCACTTCTACCTTCAGTG
AATACACTGTTACCCATGTCGGTTGTGTTGCTAAGATCAATCCTGCTGCC
CCTCTTGACAAAGTTTGTGTGCTTAGCTGTGGAATCTCCACAGGTTTTGG
GGCTACTGTTAATGTCGCCAAACCGAAGAAGGGCCAGTCTGTTGCCATTT
TTGGATTGGGTGCAGNTGGCCTCGCTGCTGCCGAAGGGGCAAGGGTATCA
GGGGCT
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