CN185672

Overview
NameCN185672
Unique NameCN185672
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length719
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2850 contig Csv1_Contig2850:52..770. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CN185672 vs. ExPASy Swiss-Prot
Match: IF5A_SULIK (Translation initiation factor 5A OS=Sulfolobus islandicus (strain M.16.4 / Kamchatka #3) GN=eIF5A PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.933e-11
Identity = 38/108 (35.19%), Postives = 59/108 (54.63%), Query Frame = 3
Query:  135 GTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFE 458
            G ++   Y+VI G PC+VVEV+ +KTGKHG AK + V I +F+G K   + P     +VP + +   Q+I    D    +  E   T  ++  PT++ L S+I+   E
Sbjct:    9 GELKVGSYVVIDGEPCRVVEVTKAKTGKHGSAKANVVAIGVFSGAKKTLMAPVDQQVEVPIIEKHIGQIIADMGDKIQVMDLETYET-FEIEKPTEDELASKIRPNAE 115          
BLAST of CN185672 vs. ExPASy Swiss-Prot
Match: IF5A_SULIA (Translation initiation factor 5A OS=Sulfolobus islandicus (strain M.16.27) GN=eIF5A PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.933e-11
Identity = 38/108 (35.19%), Postives = 59/108 (54.63%), Query Frame = 3
Query:  135 GTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFE 458
            G ++   Y+VI G PC+VVEV+ +KTGKHG AK + V I +F+G K   + P     +VP + +   Q+I    D    +  E   T  ++  PT++ L S+I+   E
Sbjct:    9 GELKVGSYVVIDGEPCRVVEVTKAKTGKHGSAKANVVAIGVFSGAKKTLMAPVDQQVEVPIIEKHIGQIIADMGDKIQVMDLETYET-FEIEKPTEDELASKIRPNAE 115          
BLAST of CN185672 vs. ExPASy Swiss-Prot
Match: IF5A_SULSO (Translation initiation factor 5A OS=Sulfolobus solfataricus GN=eif5a PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 5.136e-11
Identity = 38/108 (35.19%), Postives = 60/108 (55.56%), Query Frame = 3
Query:  135 GTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFE 458
            G ++   Y+VI G PC+VVEV+ +KTGKHG AK + V I +F+G K   + P     +VP + +   Q+I    +    +  E+  T  ++  PT++ L S+IK   E
Sbjct:    9 GELKVGSYVVIDGEPCRVVEVTKAKTGKHGSAKANVVAIGVFSGAKKTLMAPVDQQVEVPIIEKHIGQIIADMGNKIQVMDLESYET-FEIEKPTEDELASKIKPNAE 115          
BLAST of CN185672 vs. ExPASy Swiss-Prot
Match: IF5A_METJA (Translation initiation factor 5A OS=Methanocaldococcus jannaschii GN=eif5a PE=1 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 5.136e-11
Identity = 40/113 (35.40%), Postives = 63/113 (55.75%), Query Frame = 3
Query:  135 GTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDENLLSQIKDGFESGKDL 473
            G+++   Y++I G PC++V++S SK GKHG AK   VGI IF   K E + P+S   +VP + R   Q++ I  D  V ++  +  T + L LP  E +     +G E G ++
Sbjct:   10 GSLKVGQYVMIDGVPCEIVDISVSKPGKHGGAKARVVGIGIFEKVKKEFVAPTSSKVEVPIIDRRKGQVLAIMGD-MVQIM--DLQTYETLELPIPEGI-----EGLEPGGEV 114          
BLAST of CN185672 vs. ExPASy Swiss-Prot
Match: IF5A_PYRCJ (Translation initiation factor 5A OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=eIF5A PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.761e-11
Identity = 30/77 (38.96%), Postives = 46/77 (59.74%), Query Frame = 3
Query:  129 QAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISED 359
            + G +++  Y+VI G PC+VVE+  SKTGKHG AK   V + +F+G K    +P     +VP + +   Q++ IS D
Sbjct:    7 EVGELKEGSYVVIDGEPCRVVEIEKSKTGKHGSAKARVVAVGVFDGAKRTLSLPVDAQIEVPIIEKFTAQVLSISGD 83          
The following BLAST results are available for this feature:
BLAST of CN185672 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65
Match NameE-valueIdentityDescription
IF5A_SULIK3.933e-1135.19Translation initiation factor 5A OS=Sulfolobus isl... [more]
IF5A_SULIA3.933e-1135.19Translation initiation factor 5A OS=Sulfolobus isl... [more]
IF5A_SULSO5.136e-1135.19Translation initiation factor 5A OS=Sulfolobus sol... [more]
IF5A_METJA5.136e-1135.40Translation initiation factor 5A OS=Methanocaldoco... [more]
IF5A_PYRCJ8.761e-1138.96Translation initiation factor 5A OS=Pyrobaculum ca... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001L05_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001L05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185672 ID=CN185672; Name=CN185672; organism=Citrus sinensis; type=EST; length=719bp
GTTCGATTCTACCAAAATTTGGTTGTCGAAGCTCTAATTGTGAATCGCAG
GAACCAGACGATATGTCGGACGAAGAGCACCACTTTGAGTCGAAGGCCGA
CGCCGGCGCCTCCAAGACATTTCCTCAGCAGGCCGGAACCATCCGCAAAA
ACGGTTATATCGTCATCAAAGGTCGTCCTTGCAAGGTTGTTGAGGTTTCA
ACTTCCAAGACTGGAAAGCACGGTCATGCTAAGTGTCACTTTGTAGGAAT
TGACATCTTCAATGGCAAGAAGCTTGAGGATATTGTTCCCTCTTCCCACA
ACTGTGATGTTCCTCATGTCACTCGCACTGACTACCAGCTGATTGATATC
TCTGAGGATGGATTTGTTAGCCTGCTGACTGAGAATGGCAATACCAAGGA
TGACCTGAGACTTCCAACTGATGAAAATCTGCTTTCACAGATCAAGGATG
GATTTGAGAGTGGGAAAGATCTGGTTGTCTCAGTTCAGTGTGCTATGGGA
GAGGAGCAGATCAATGCCCTGAAGGACATTGGCCCCAAGTAAAAGACTTT
TGCTTGATCGAGAATGCAGATTGCTGAAAGCTGTGGGAAGGTGGATAGTA
GTGTAGCTTTACTGGTGTCCGGTTTCAAGATGAATGCCATCATTTCATAT
AAAATGACTCTTACCATATTATTAATTGTTTTCTATCGACAGTATTTTTA
TTCTGTTTGATTGCTTGAA
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