CN185537

Overview
NameCN185537
Unique NameCN185537
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length692
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9569 contig Csv1_Contig9569:9..700. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UBL1_SCHPO (Ubiquitin-like protein 1 OS=Schizosaccharomyces pombe GN=ubl1 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.508e-17
Identity = 40/76 (52.63%), Postives = 56/76 (73.68%), Query Frame = 1
Query:   52 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          

HSP 2 Score: 88.1965 bits (217), Expect = 4.508e-17
Identity = 40/76 (52.63%), Postives = 56/76 (73.68%), Query Frame = 1
Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y+++  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLEGGSVLHLVLALRGG 76          

HSP 3 Score: 70.4774 bits (171), Expect = 9.728e-12
Identity = 30/61 (49.18%), Postives = 45/61 (73.77%), Query Frame = 1
Query:  508 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 690
            M I VKTLTGK I L+++ +D +  +K ++++KEGIPP QQRLI+AGKQ+ D +    Y++
Sbjct:    1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHL 61          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UCRP_MOUSE (Ubiquitin cross-reactive protein OS=Mus musculus GN=Isg15 PE=1 SV=4)

HSP 1 Score: 83.5741 bits (205), Expect = 1.110e-15
Identity = 51/149 (34.23%), Postives = 79/149 (53.02%), Query Frame = 1
Query:   64 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLR-LRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            VK L G    + V +S T+  +K +I  K G+P  QQRL      L+DG TL+   +   ST+ LV++     + I V+   G +   EV  + T+D +K K+  +E +  DQ  L F G+ +ED   L +Y ++ + T+   LRLRGG
Sbjct:    7 VKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGG 155          

HSP 2 Score: 72.4034 bits (176), Expect = 2.560e-12
Identity = 44/134 (32.84%), Postives = 69/134 (51.49%), Query Frame = 1
Query:  292 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLR-LRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 690
            VK L G    + V +S T+  +K +I  K G+P  QQRL      L+DG TL+   +   ST+ LV++     + I V+   G +   EV  + T+D +K K+  +E +  DQ  L F G+ +ED   L +Y +
Sbjct:    7 VKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGL 140          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: RUB1_YEAST (NEDD8-like protein RUB1 OS=Saccharomyces cerevisiae GN=RUB1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.110e-15
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 1
Query:   52 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          

HSP 2 Score: 83.5741 bits (205), Expect = 1.110e-15
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 1
Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++ +   LHLVL LRGG
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGG 76          

HSP 3 Score: 68.5514 bits (166), Expect = 3.697e-11
Identity = 31/61 (50.82%), Postives = 47/61 (77.05%), Query Frame = 1
Query:  508 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 690
            M + VKTLTGK I++E++ SD + ++K  +++KEGIPP QQRLIF GKQ++D  T+ D ++
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHL 61          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UBD_HUMAN (Ubiquitin D OS=Homo sapiens GN=UBD PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 9.400e-15
Identity = 49/156 (31.41%), Postives = 81/156 (51.92%), Query Frame = 1
Query:   58 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFVKTL--TGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            + V++     +T +    D++  +K  ++ K  +P   Q L+   K L+  R+L+ Y I KE T+HL L++       + +F+       K   L+V  S ++  VKA I+ K GI P+ Q +   GK+LEDG+ +ADY I+K + L L     GG
Sbjct:   10 VHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGIRKGNLLFLACYCIGG 165          

HSP 2 Score: 74.3294 bits (181), Expect = 6.737e-13
Identity = 44/141 (31.21%), Postives = 74/141 (52.48%), Query Frame = 1
Query:  286 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFVKTL--TGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNI 690
            + V++     +T +    D++  +K  ++ K  +P   Q L+   K L+  R+L+ Y I KE T+HL L++       + +F+       K   L+V  S ++  VKA I+ K GI P+ Q +   GK+LEDG+ +ADY I
Sbjct:   10 VHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGI 150          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UCRP_HUMAN (Interferon-induced 17 kDa protein OS=Homo sapiens GN=ISG15 PE=1 SV=5)

HSP 1 Score: 78.1814 bits (191), Expect = 4.665e-14
Identity = 52/151 (34.44%), Postives = 81/151 (53.64%), Query Frame = 1
Query:   64 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF--AGKQLEDGRTLADYNIQKESTLHLVL-RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            VK L G    + + SS ++  +KA+I  K G+   QQRL    +G  L+D   LA   +   ST+ LV+ +    + I V+   G++ T EV  + T+ ++K ++   EG+  D   L F GK LED   L +Y ++  ST+ + LRLRGG
Sbjct:    7 VKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG 157          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UBD_RAT (Ubiquitin D OS=Rattus norvegicus GN=Ubd PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.501e-12
Identity = 44/146 (30.14%), Postives = 72/146 (49.32%), Query Frame = 1
Query:   88 ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL------RGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            +T +   SD +  +   I+ +  +    Q L+   K L+  R L+ Y I KE+T+HL L++         + +      G+   L V  S ++  VK  I++   +PP +Q +   GK+LEDG+ +ADYNI+  S L L     GG
Sbjct:   16 MTFDTTMSDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRALSSYGIDKENTIHLTLKVVKPSDEELPLSLVESGDEGQRHLLRVRRSSSVAQVKEMIENVTAVPPKKQIVNCNGKRLEDGKIMADYNIKSGSLLFLTAHCIGG 161          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UCRP_BOVIN (Ubiquitin cross-reactive protein OS=Bos taurus GN=ISG15 PE=2 SV=3)

HSP 1 Score: 70.8626 bits (172), Expect = 7.448e-12
Identity = 47/150 (31.33%), Postives = 78/150 (52.00%), Query Frame = 1
Query:   64 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQ--LEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            VK L G+ I + +  S T+  +K  I  K  +P  QQRL     +  L++G  L    ++  ST+ LV++    + I V+   G++   EV+   T+  +K ++  KE +  DQ  L F G+ ++D   L +Y + K  T+ + LRLRGG
Sbjct:    7 VKMLGGQEILVPLRDSMTVSELKQFIAQKINVPAFQQRLAHLDSREVLQEGVPLVLQGLRAGSTVLLVVQ--NCISILVRNDKGRSSPYEVQLKQTVAELKQQVCQKERVQADQFWLSFEGRPMDDEHPLEEYGLMKGCTVFMNLRLRGG 154          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: UBD_MOUSE (Ubiquitin D OS=Mus musculus GN=Ubd PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.271e-11
Identity = 44/148 (29.73%), Postives = 73/148 (49.32%), Query Frame = 1
Query:   82 KTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRL----RGGMQIFV--KTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            + +T E   +D +  +   I+ +  +    Q L+   K L+  R L+ Y I KE+T+HL L++       + +F+      G+   L V  S ++  VK  I+    + P +Q +   GK+LEDG+ +ADYNI+  S L L     GG
Sbjct:   15 RLMTFETTENDKVKKINEHIRSQTKVSVQDQILLLDSKILKPHRKLSSYGIDKETTIHLTLKVVKPSDEELPLFLVESKNEGQRHLLRVRRSSSVAQVKEMIESVTSVIPKKQVVNCNGKKLEDGKIMADYNIKSGSLLFLTTHCTGG 162          
BLAST of CN185537 vs. ExPASy Swiss-Prot
Match: ANUB1_HUMAN (AN1-type zinc finger and ubiquitin domain-containing protein 1 OS=Homo sapiens GN=ANUBL1 PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 3.697e-11
Identity = 53/161 (32.92%), Postives = 82/161 (50.93%), Query Frame = 1
Query:   52 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF-AGKQLE--------DGRTLADYNIQKESTLHL-VLRLRG 504
            M++F++TLTG    L V   +T+ +VKAKI+  EGIP  +Q LI+   +LE+   L DYNI +  TL LVL +RGG    + T    T     + ++ +D+ + ++ +K         L++  G QL         DG      +  K+   HL VLR +G
Sbjct:   28 MELFIETLTGTCFELRVSPFETVISVKAKIRRLEGIPICRQHLIWNNMELENDYCLNDYNISEGCTLKLVLAMRGGP---INTRRVPTDDPLRKMAEYLDSSRVEVWEKTSCSKQVTFLVYQEGDQLNFFPAVDRGDGTLTPLSDSSKKIDFHLHVLRRKG 185          

HSP 2 Score: 68.1662 bits (165), Expect = 4.828e-11
Identity = 36/76 (47.37%), Postives = 50/76 (65.79%), Query Frame = 1
Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 507
            M++F++TLTG    L V   +T+ +VKAKI+  EGIP  +Q LI+   +LE+   L DYNI +  TL LVL +RGG
Sbjct:   28 MELFIETLTGTCFELRVSPFETVISVKAKIRRLEGIPICRQHLIWNNMELENDYCLNDYNISEGCTLKLVLAMRGG 103          
The following BLAST results are available for this feature:
BLAST of CN185537 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
UBL1_SCHPO4.508e-1752.63Ubiquitin-like protein 1 OS=Schizosaccharomyces po... [more]
UCRP_MOUSE1.110e-1534.23Ubiquitin cross-reactive protein OS=Mus musculus G... [more]
RUB1_YEAST1.110e-1552.63NEDD8-like protein RUB1 OS=Saccharomyces cerevisia... [more]
UBD_HUMAN9.400e-1531.41Ubiquitin D OS=Homo sapiens GN=UBD PE=1 SV=2[more]
UCRP_HUMAN4.665e-1434.44Interferon-induced 17 kDa protein OS=Homo sapiens ... [more]
UBD_RAT1.501e-1230.14Ubiquitin D OS=Rattus norvegicus GN=Ubd PE=2 SV=1[more]
UCRP_BOVIN7.448e-1231.33Ubiquitin cross-reactive protein OS=Bos taurus GN=... [more]
UBD_MOUSE1.271e-1129.73Ubiquitin D OS=Mus musculus GN=Ubd PE=2 SV=1[more]
ANUB1_HUMAN3.697e-1132.92AN1-type zinc finger and ubiquitin domain-containi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0001H23_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001H23, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185537 ID=CN185537|Name=CN185537|organism=Citrus sinensis|type=EST|length=692bp
GAGGTTCACCTTTAATTCGATAAAAGGCCTCCTTCTGTTCTCTCTGTCAA
GATGCAAATCTTTGTGAAAACTCTCACCGGCAAGACAATAACCCTTGAGG
TTGAAAGCTCCGACACCATTGATAATGTAAAAGCCAAGATCCAGGACAAG
GAAGGGATCCCACCAGATCAGCAGAGGCTTATCTTTGCTGGTAAACAGCT
CGAGGATGGTCGCACACTTGCTGACTATAATATCCAGAAGGAATCAACCC
TCCACTTGGTCCTTCGACTTAGGGGTGGTATGCAGATCTTTGTAAAGACC
CTCACTGGCAAGACGATTACTCTTGAGGTGGAAAGCTCCGACACCATTGA
CAATGTGAAAGCCAAGATTCAAGACAAGGAAGGGATCCCACCAGACCAAC
AGAGACTTATCTTTGCCGGTAAACAGCTCGAGGATGGTCGCACACTTGCT
GATTATAACATCCAGAAGGAGTCAACCCTCCACTTGGTCCTCCGTCTCCG
TGGTGGTATGCAGATCTTTGTAAAGACCCTCACTGGAAAGACTATCACCC
TTGAGGTGGAAAGCTCCGACACCATTGACAATGTCAAAGCCAAAATTCAG
GATAAGGAAGGGATCCCACCAGACCAGCAAAGGCTCATCTTTGCTGGTAA
ACAACTAGAGGATGGACGCACACTTGCAGACTACAACATCCA
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