CN181477

Overview
NameCN181477
Unique NameCN181477
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length633
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3890 contig Csv1_Contig3890:239..871. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA4_HUMAN (Protein disulfide-isomerase A4 OS=Homo sapiens GN=PDIA4 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 2.148e-20
Identity = 44/92 (47.83%), Postives = 62/92 (67.39%), Query Frame = -3 
Query:  365 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYE---GDRTKEDIVDFIENNRDK 631
            VL+EFYAPWCGHCK+L P+ + +A  Y+    +VIAK DATAND+P D ++V+G+PT++F  +  K  P +   GDR  E +  FIE +  K
Sbjct:  546 VLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATK 637

HSP 2 Score: 82.0333 bits (201), Expect = 2.718e-15
Identity = 39/81 (48.15%), Postives = 53/81 (65.43%), Query Frame = -3 
Query:  392 VLLEFYAPWCGHCKKLAPILDEVA-VSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIV 631
            VLLEFYAPWCGHCK+ AP  +++A +    D  + +AK DAT+  +    F+V GYPT+      G+ V YEG RT+E+IV
Sbjct:   82 VLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILK-KGQAVDYEGSRTQEEIV 161

HSP 3 Score: 73.1738 bits (178), Expect = 1.262e-12
Identity = 39/85 (45.88%), Postives = 51/85 (60.00%), Query Frame = -3 
Query:  383 VLLEFYAPWCGHCKKLAPILDEVAVSY-QNDADVVIAKFDATANDIPGDTFEVQGYPTV-FFRSASGKTVPYEGDRTKEDIVDFI 631
            +L+EFYAPWCGHCKKLAP  ++ A    +    + +AK DATA       F+V GYPT+  FR   G+   Y G R K  IVD++
Sbjct:  197 ILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFR--KGRPYDYNGPREKYGIVDYM 279
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDI_HUMIN (Protein disulfide-isomerase OS=Humicola insolens PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 3.664e-20
Identity = 55/110 (50.00%), Postives = 68/110 (61.82%), Query Frame = -3 
Query:  320 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDA---DVVIAKFDATANDIPGDTFEVQGYPTV--FFRSASGKTVPYEGDRTKEDIVDFI-ENNRDKAAPKETVKEESGKDE 631
            VL+EFYAPWCGHCK LAP  +E+   Y        VVIAK DATAND+P    E+QG+PT+  +   A G+ V Y G RT ED++ FI EN + KAA  E  +E S   E
Sbjct:  376 VLIEFYAPWCGHCKALAPKYEELGALYAKSEFKDRVVIAKVDATANDVPD---EIQGFPTIKLYPAGAKGQPVTYSGSRTVEDLIKFIAENGKYKAAISEDAEETSSATE 482
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDI2_CAEEL (Protein disulfide-isomerase 2 OS=Caenorhabditis elegans GN=pdi-2 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.066e-19
Identity = 48/106 (45.28%), Postives = 67/106 (63.21%), Query Frame = -3 
Query:  320 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTV-FFRSASGKTVPYEGDRTKEDIVDFIE-NNRDKAAPKETVKEESGKDE 631
            VL+EFYAPWCGHCK+LAP  D++   + +D  +VIAK D+T N++  +  ++Q +PT+ FF + S K V Y GDRT E    F+E N ++ A   E  K E   DE
Sbjct:  384 VLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEV--EDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETNGKEGAGASEEEKAEEEADE 487

HSP 2 Score: 72.4034 bits (176), Expect = 2.153e-12
Identity = 37/93 (39.78%), Postives = 54/93 (58.06%), Query Frame = -3 
Query:  359 VLLEFYAPWCGHCKKLAPILDEVAVSYQND-ADVVIAKFDATANDIPGDTFEVQGYPTV-FFRSASGKTVPYEGDRTKEDIVDFIENNRDKAA 631
            +L+EFYAPWCGHCK LAP   + A   + + +D+ + K DAT +      FEV+GYPT+  FR  +GK   Y G R  + I+ +++      A
Sbjct:   43 ILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFR--NGKPQEYNGGRDHDSIIAWLKKKTGPVA 133
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDI_ASPOR (Protein disulfide-isomerase OS=Aspergillus oryzae GN=pdiA PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 4.051e-19
Identity = 46/88 (52.27%), Postives = 59/88 (67.05%), Query Frame = -3 
Query:  374 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTV--FFRSASGKTVPYEGDRTKEDIVDFIENN 631
            VLLEFYAPWCGHCK LAP  +E+A  Y++  +V IAK DATAND+P     + G+PT+  F   A    V YEG RT ED+ +F++ N
Sbjct:  384 VLLEFYAPWCGHCKALAPKYEELASLYKDIPEVTIAKIDATANDVPD---SITGFPTIKLFAAGAKDSPVEYEGSRTVEDLANFVKEN 468
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA4_MOUSE (Protein disulfide-isomerase A4 OS=Mus musculus GN=Pdia4 PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 4.051e-19
Identity = 46/102 (45.10%), Postives = 64/102 (62.75%), Query Frame = -3 
Query:  335 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYE---GDRTKEDIVDFIENNRDKAAPKETVKEE 631
            VL+EFYAPWCGHCK+L PI   +   Y+   D+VIAK DATANDI  D ++V+G+PT++F  +  K  P +   G+R  E +  FI+   + A  +   KEE
Sbjct:  539 VLIEFYAPWCGHCKQLEPIYTSLGKKYKGQKDLVIAKMDATANDITNDQYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFID---EHATKRSRTKEE 637

HSP 2 Score: 83.1889 bits (204), Expect = 1.220e-15
Identity = 39/81 (48.15%), Postives = 55/81 (67.90%), Query Frame = -3 
Query:  392 VLLEFYAPWCGHCKKLAPILDEVAVSYQ-NDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIV 631
            VLLEFYAPWCGHCK+ AP  +++A + + ND  + +AK DAT+  +    F+V GYPT+      G+ V Y+G RT+E+IV
Sbjct:   75 VLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILK-KGQAVDYDGSRTQEEIV 154

HSP 3 Score: 71.2478 bits (173), Expect = 4.797e-12
Identity = 38/85 (44.71%), Postives = 50/85 (58.82%), Query Frame = -3 
Query:  383 VLLEFYAPWCGHCKKLAPILDEVAVSY-QNDADVVIAKFDATANDIPGDTFEVQGYPTV-FFRSASGKTVPYEGDRTKEDIVDFI 631
            +L+EFYAPWCGHCKKLAP  ++ A    +    + +AK DAT        F+V GYPT+  FR   G+   Y G R K  IVD++
Sbjct:  190 ILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFR--KGRPFDYNGPREKYGIVDYM 272
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA4_RAT (Protein disulfide-isomerase A4 OS=Rattus norvegicus GN=Pdia4 PE=1 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 5.291e-19
Identity = 45/102 (44.12%), Postives = 64/102 (62.75%), Query Frame = -3 
Query:  335 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYE---GDRTKEDIVDFIENNRDKAAPKETVKEE 631
            VL+EFYAPWCGHCK+L P+   +   Y+   D+VIAK DATANDI  D ++V+G+PT++F  +  K  P +   G+R  E +  FI+   + A  +   KEE
Sbjct:  544 VLIEFYAPWCGHCKQLEPVYTSLGKKYKGQKDLVIAKMDATANDITNDRYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFID---EHATKRSRTKEE 642

HSP 2 Score: 83.1889 bits (204), Expect = 1.220e-15
Identity = 39/81 (48.15%), Postives = 55/81 (67.90%), Query Frame = -3 
Query:  392 VLLEFYAPWCGHCKKLAPILDEVAVSYQ-NDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIV 631
            VLLEFYAPWCGHCK+ AP  +++A + + ND  + +AK DAT+  +    F+V GYPT+      G+ V Y+G RT+E+IV
Sbjct:   80 VLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILK-KGQAVDYDGSRTQEEIV 159

HSP 3 Score: 71.2478 bits (173), Expect = 4.797e-12
Identity = 38/85 (44.71%), Postives = 50/85 (58.82%), Query Frame = -3 
Query:  383 VLLEFYAPWCGHCKKLAPILDEVAVSY-QNDADVVIAKFDATANDIPGDTFEVQGYPTV-FFRSASGKTVPYEGDRTKEDIVDFI 631
            +L+EFYAPWCGHCKKLAP  ++ A    +    + +AK DAT        F+V GYPT+  FR   G+   Y G R K  IVD++
Sbjct:  195 ILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFR--KGRPFDYNGPREKYGIVDYM 277
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA3_RAT (Protein disulfide-isomerase A3 OS=Rattus norvegicus GN=Pdia3 PE=1 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 6.911e-19
Identity = 46/104 (44.23%), Postives = 64/104 (61.54%), Query Frame = -3 
Query:  326 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVP--YEGDRTKEDIVDFIENNRDKAAPKETVKEESGK 631
            VL+EFYAPWCGHCK L P   E+      D ++VIAK DATAND+P   +EV+G+PT++F  A+ K  P  YEG R   D + +++    +A     ++EE  K
Sbjct:  397 VLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVP-SPYEVKGFPTIYFSPANKKLTPKKYEGGRELNDFISYLQR---EATNPPIIQEEKPK 496

HSP 2 Score: 68.9366 bits (167), Expect = 2.381e-11
Identity = 46/139 (33.09%), Postives = 66/139 (47.48%), Query Frame = -3 
Query:  230 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIVDFIENNRDKAAPKETVKEE-----SGKDEL*EGMGASLLESRAHSRLARVWRMLYQNYQ 631
            +L+EF+APWCGHCK+LAP  +  A   +    V +AK D TAN    + + V GYPT+       +   Y+G RT + IV  ++     A+     ++E     S KD    G    L  S  HS   +    L  NY+
Sbjct:   48 MLVEFFAPWCGHCKRLAPEYEAAATRLK--GIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEDEFKKFISDKDASVVGFFRDLF-SDGHSEFLKAASNLRDNYR 183
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA3_MOUSE (Protein disulfide-isomerase A3 OS=Mus musculus GN=Pdia3 PE=1 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 6.911e-19
Identity = 46/104 (44.23%), Postives = 64/104 (61.54%), Query Frame = -3 
Query:  326 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVP--YEGDRTKEDIVDFIENNRDKAAPKETVKEESGK 631
            VL+EFYAPWCGHCK L P   E+      D ++VIAK DATAND+P   +EV+G+PT++F  A+ K  P  YEG R   D + +++    +A     ++EE  K
Sbjct:  397 VLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVP-SPYEVKGFPTIYFSPANKKLTPKKYEGGRELNDFISYLQR---EATNPPIIQEEKPK 496

HSP 2 Score: 70.0922 bits (170), Expect = 1.069e-11
Identity = 47/139 (33.81%), Postives = 66/139 (47.48%), Query Frame = -3 
Query:  230 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIVDFIENNRDKAAPKETVKEE-----SGKDEL*EGMGASLLESRAHSRLARVWRMLYQNYQ 631
            +L+EF+APWCGHCK+LAP  +  A   +    V +AK D TAN    + + V GYPT+       +   Y+G RT + IV  ++     A+     +EE     S KD    G    L  S  HS   +    L  NY+
Sbjct:   48 MLVEFFAPWCGHCKRLAPEYEAAATRLK--GIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASVVGFFRDLF-SDGHSEFLKAASNLRDNYR 183
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA3_BOVIN (Protein disulfide-isomerase A3 OS=Bos taurus GN=PDIA3 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 9.026e-19
Identity = 48/104 (46.15%), Postives = 66/104 (63.46%), Query Frame = -3 
Query:  326 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVP--YEGDRTKEDIVDFIENNRDKAAPKETVKEESGK 631
            VL+EFYAPWCGHCK L P   E+    + D ++VIAK DATAND+P   +EV+G+PT++F  A+ K  P  YEG R   ++ DFI   + +A     ++EE  K
Sbjct:  397 VLIEFYAPWCGHCKNLEPKYKELGEKLRKDPNIVIAKMDATANDVP-SPYEVRGFPTIYFSPANKKQNPKKYEGGR---ELSDFISYLKREATNPPVIQEEKPK 496

HSP 2 Score: 73.1738 bits (178), Expect = 1.262e-12
Identity = 49/139 (35.25%), Postives = 68/139 (48.92%), Query Frame = -3 
Query:  230 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIVDFIENNRDKAAPKETVKEE-----SGKDEL*EGMGASLLESRAHSRLARVWRMLYQNYQ 631
            +L+EF+APWCGHCKKLAP  +  A   +    V +AK D TAN    + + V GYPT+       ++  Y+G RT + IV  ++     A+     +EE     S KD    G    L  S AHS   +    L  NY+
Sbjct:   48 MLVEFFAPWCGHCKKLAPEYEAAATRLK--GIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEESGAYDGPRTADGIVSHLKKQAGPASVPLKSEEEFEKFISDKDASVVGFFKDLF-SEAHSEFLKAASNLRDNYR 183
[Close]
BLAST of CN181477 vs. ExPASy Swiss-Prot
Match: PDIA3_PONAB (Protein disulfide-isomerase A3 OS=Pongo abelii GN=PDIA3 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.179e-18
Identity = 46/104 (44.23%), Postives = 64/104 (61.54%), Query Frame = -3 
Query:  326 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVP--YEGDRTKEDIVDFIENNRDKAAPKETVKEESGK 631
            VL+EFYAPWCGHCK L P   E+      D ++VIAK DATAND+P   +EV+G+PT++F  A+ K  P  YEG R   D + +++    +A     ++EE  K
Sbjct:  397 VLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVP-SPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQR---EATNPPVIQEEKPK 496

HSP 2 Score: 69.707 bits (169), Expect = 1.396e-11
Identity = 47/139 (33.81%), Postives = 66/139 (47.48%), Query Frame = -3 
Query:  230 VLLEFYAPWCGHCKKLAPILDEVAVSYQNDADVVIAKFDATANDIPGDTFEVQGYPTVFFRSASGKTVPYEGDRTKEDIVDFIENNRDKAAPKETVKEE-----SGKDEL*EGMGASLLESRAHSRLARVWRMLYQNYQ 631
            +L+EF+APWCGHCK+LAP  +  A   +    V +AK D TAN    + + V GYPT+       +   Y+G RT + IV  ++     A+     +EE     S KD    G       S AHS   +    L  NY+
Sbjct:   48 MLVEFFAPWCGHCKRLAPEYEAAATRLK--GIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSF-SEAHSEFLKAASNLRDNYR 183
The following BLAST results are available for this feature:
BLAST of CN181477 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65
Match NameE-valueIdentityDescription
PDIA4_HUMAN2.148e-2047.83Protein disulfide-isomerase A4 OS=Homo sapiens GN=... [more]
PDI_HUMIN3.664e-2050.00Protein disulfide-isomerase OS=Humicola insolens P... [more]
PDI2_CAEEL1.066e-1945.28Protein disulfide-isomerase 2 OS=Caenorhabditis el... [more]
PDI_ASPOR4.051e-1952.27Protein disulfide-isomerase OS=Aspergillus oryzae ... [more]
PDIA4_MOUSE4.051e-1945.10Protein disulfide-isomerase A4 OS=Mus musculus GN=... [more]
PDIA4_RAT5.291e-1944.12Protein disulfide-isomerase A4 OS=Rattus norvegicu... [more]
PDIA3_RAT6.911e-1944.23Protein disulfide-isomerase A3 OS=Rattus norvegicu... [more]
PDIA3_MOUSE6.911e-1944.23Protein disulfide-isomerase A3 OS=Mus musculus GN=... [more]
PDIA3_BOVIN9.026e-1946.15Protein disulfide-isomerase A3 OS=Bos taurus GN=PD... [more]
PDIA3_PONAB1.179e-1844.23Protein disulfide-isomerase A3 OS=Pongo abelii GN=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001A04_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001A04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181477 ID=CN181477; Name=CN181477; organism=Citrus sinensis; type=EST; length=633bp
TATCCATTTCGGATTTAACACGCAGTAAGTTACTCCTAGGCGCCGAAAAT
ACTAAAATATCACAACTATGCGAATACCAAAAAGTACTGCAGCTACAAAT
GCCGAAAATGCCTCCCCATTTTATTCACGGGAACAGAACAGATCTGTAAA
GGGAAATGGCCTAAGAAAAAGCTATAAGGGGGGATATAGGACAAGGGGGA
GGAGAACATCAGGCATTGTTTTGTCGACACTGATAGTTTTGGTATAACAT
CCGCCAAACACGCGCCAACCGACTATGTGCACGGCTCTCTAATAAACTCG
CACCCATTCCTTCTTAGAGCTCATCTTTTCCAGATTCCTCTTTTACAGTT
TCCTTGGGGGCAGCTTTGTCCCTGTTATTTTCAATGAAGTCAACAATATC
TTCTTTGGTCCTATCCCCCTCATAGGGCACTGTCTTTCCACTAGCAGATC
GGAAGAACACGGTTGGGTAACCTTGGACTTCAAAAGTGTCACCAGGAATA
TCATTTGCGGTTGCATCAAATTTTGCAATAACAACATCAGCATCGTTTTG
ATATGAGACAGCAACTTCATCCAAGATTGGAGCCAACTTTTTGCAGTGTC
CACACCAAGGAGCGTAAAACTCGAGCAAAACAT
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