CN188561

Overview
NameCN188561
Unique NameCN188561
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length707
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: SPAST_DROMO (Spastin OS=Drosophila mojavensis GN=spas PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 54/142 (38.03%), Postives = 83/142 (58.45%), Query Frame = 3
Query:   78 RAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGF---EQDKKVVVIAATNRKQDLDPALISRFDSMITFGLPDHENRQ 494
            + +L  GPPG GKT  AR +A +     + +    + SKY G+ E+L+  +F++A  L   +IIF+DEVDS    R S  HEA+RR+ +  L + DG     +  ++VV+AATNR Q+LD A + RF   +   LP+ + R+
Sbjct:  524 KGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHL-QPSIIFIDEVDSLLSERSSNEHEASRRLKTEFLVEFDGLPGNPEGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPEVQTRE 664          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: SPAST_DROGR (Spastin OS=Drosophila grimshawi GN=spas PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 54/142 (38.03%), Postives = 82/142 (57.75%), Query Frame = 3
Query:   78 RAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGF---EQDKKVVVIAATNRKQDLDPALISRFDSMITFGLPDHENRQ 494
            + +L  GPPG GKT  AR +A +     + +    + SKY G+ E+L+  +F++A  +   +IIF+DEVDS    R S  HEA+RR+ +  L + DG        ++VV+AATNR Q+LD A + RF   +   LPD + R+
Sbjct:  541 KGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHM-QPSIIFIDEVDSLLSERSSNEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDVQTRE 681          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_THEON (Proteasome-activating nucleotidase OS=Thermococcus onnurineus (strain NA1) GN=pan PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 64/172 (37.21%), Postives = 94/172 (54.65%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAK 518
            PE+++ + +E         P+ VL  GPPG GKT  A+ +A++     + V    ++ K+ GE  RL+ ++F LA E    AIIF+DE+D+    R  E     R   R L  LL ++DGF+    V +IAATNR   LDPAL+   RFD +I   LP+ ++R EI   + K
Sbjct:  162 PELFEKVGIEP--------PKGVLLYGPPGCGKTLMAKALAHEVNATFIRVVGSELVRKFIGEGARLVHELFELAKE-KAPAIIFIDEIDAIGAKRMDETTGGEREVNRTLMQLLAEMDGFDPSGNVKIIAATNRPDILDPALLRPGRFDRLIEVPLPNFKSRLEILKIHTK 324          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS4_ORYSJ (26S protease regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica GN=TBP2 PE=2 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 67/204 (32.84%), Postives = 102/204 (50.00%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVAR---DSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PE+Y+DI +          P+ V+  G PGTGKT  A+ +AN      + V    ++ KY G+  +L+ ++F +A+EL + +I+F+DE+D+    R    S      +R +  LL Q+DGF+    V VI ATNR + LDPAL+   R D  I F LPD + R+ I   +                      SG DI+ +C +A
Sbjct:  216 PELYEDIGIRP--------PKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADEL-SPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSKMTLADDVNLEEFVMTKDEFSGADIKAICTEA 410          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS4B_ARATH (26S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana GN=RPT2B PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 66/204 (32.35%), Postives = 104/204 (50.98%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVAR---DSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PE+Y+DI ++         P+ V+  G PGTGKT  A+ +AN      + V    ++ KY G+  +L+ ++F +A++L + +I+F+DE+D+    R   +S      +R +  LL Q+DGF+    V VI ATNR + LDPAL+   R D  I F LPD + R+ I   +                      SG DI+ +C +A
Sbjct:  211 PELYEDIGIKP--------PKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDL-SPSIVFIDEIDAVGTKRYDANSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGADIKAICTEA 405          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS10_SCHPO (Probable 26S protease subunit rpt4 OS=Schizosaccharomyces pombe GN=rpt4 PE=2 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 62/147 (42.18%), Postives = 81/147 (55.10%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI 500
            P+ VL  GPPGTGKT  AR +A   GV  + V    ++ KY GES R++ ++F  A E     +IF+DE+D+    R SE   A R   R L  LL Q+DGF+   +  +I ATNR   LDPAL+   R D  I   LP+   R EI
Sbjct:  166 PKGVLLYGPPGTGKTLLARAVAASLGVNFLKVVSSAIVDKYIGESARIIREMFGYAKE-HEPCVIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDYLGQTKIIMATNRPDTLDPALLRPGRLDRKIEIPLPNEVGRMEI 311          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: ATD2B_HUMAN (ATPase family AAA domain-containing protein 2B OS=Homo sapiens GN=ATAD2B PE=1 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 67/168 (39.88%), Postives = 88/168 (52.38%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEV-----VMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEI 500
            PE+++        KF+   PR  LF GPPGTGKT  AR +AN+       V   +      +SK+ GESER L  +F  A  L   +IIF DE+D  A  R S   +    I+S LL  +DG +   ++VVI ATNR   +DPAL    RFD    F LPD + R+ I
Sbjct:  423 PEIFE--------KFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAY-LMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHI 581          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_METM6 (Proteasome-activating nucleotidase OS=Methanococcus maripaludis (strain C6 / ATCC BAA-1332) GN=pan PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 7.008e-21
Identity = 66/204 (32.35%), Postives = 103/204 (50.49%), Query Frame = 3
Query:   15 SPEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAK--HXXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            +PE+++ + +          P+ VL  GPPGTGKT  A+ +A +     + V    ++ K+ GE  +L+  VF LA E  +  IIF+DE+D+ A  R   +    R   R L  LL ++DGF+    V +IAATNR   LDPA++   RFD +I   +PD + R +I   + +  +               M G D++ VC +A
Sbjct:  170 NPELFEKVGIVP--------PKGVLLYGPPGTGKTLLAKAVAYETNASFVRVVGSELVKKFIGEGAKLVRDVFKLAKE-KSPCIIFIDEIDAVASKRTESLTGGDREVQRTLMQLLAEMDGFDSRGDVKIIAATNRPDILDPAILRPGRFDRIIEISMPDEDGRLDILKIHTEKMNLKGVDLREVAKLAENMVGADLKAVCTEA 364          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_METM5 (Proteasome-activating nucleotidase OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=pan PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 7.008e-21
Identity = 66/204 (32.35%), Postives = 103/204 (50.49%), Query Frame = 3
Query:   15 SPEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAK--HXXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            +PE+++ + +          P+ VL  GPPGTGKT  A+ +A +     + V    ++ K+ GE  +L+  VF LA E  +  IIF+DE+D+ A  R   +    R   R L  LL ++DGF+    V +IAATNR   LDPA++   RFD +I   +PD + R +I   + +  +               M G D++ VC +A
Sbjct:  170 NPELFEKVGIVP--------PKGVLLYGPPGTGKTLLAKAVAYETNASFVRVVGSELVKKFIGEGAKLVRDVFKLAKE-KSPCIIFIDEIDAVASKRTESLTGGDREVQRTLMQLLAEMDGFDSRGDVKIIAATNRPDILDPAILRPGRFDRIIEISMPDEDGRLDILKIHTEKMNLKGVDLREVAKIAENMVGADLKAVCTEA 364          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_CAEEL (26S protease regulatory subunit 7 OS=Caenorhabditis elegans GN=rpt-1 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 7.008e-21
Identity = 67/204 (32.84%), Postives = 98/204 (48.04%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMH---EATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PE Y ++ +E         P+ VL  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       +IF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LDPAL+   R D  + F LPD   R  I   +AK                    +G +IR VC +A
Sbjct:  200 PERYVNLGIEP--------PKGVLLYGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACLIFFDEIDAVGGARFDDGQGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKVEFALPDLAGRAHILKIHAKQMSVERDIRYDLLARLCPNSTGAEIRSVCTEA 394          
The following BLAST results are available for this feature:
BLAST of CN188561 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 381
Match NameE-valueIdentityDescription
SPAST_DROMO5.366e-2138.03Spastin OS=Drosophila mojavensis GN=spas PE=3 SV=1[more]
SPAST_DROGR5.366e-2138.03Spastin OS=Drosophila grimshawi GN=spas PE=3 SV=1[more]
PSMR_THEON5.366e-2137.21Proteasome-activating nucleotidase OS=Thermococcus... [more]
PRS4_ORYSJ5.366e-2132.8426S protease regulatory subunit 4 homolog OS=Oryza... [more]
PRS4B_ARATH5.366e-2132.3526S proteasome regulatory subunit 4 homolog B OS=A... [more]
PRS10_SCHPO5.366e-2142.18Probable 26S protease subunit rpt4 OS=Schizosaccha... [more]
ATD2B_HUMAN5.366e-2139.88ATPase family AAA domain-containing protein 2B OS=... [more]
PSMR_METM67.008e-2132.35Proteasome-activating nucleotidase OS=Methanococcu... [more]
PSMR_METM57.008e-2132.35Proteasome-activating nucleotidase OS=Methanococcu... [more]
PRS7_CAEEL7.008e-2132.8426S protease regulatory subunit 7 OS=Caenorhabditi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006E21_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006E21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188561 ID=CN188561; Name=CN188561; organism=Citrus sinensis; type=EST; length=707bp
ACATTTTAGCTTCAAGCCCTGAAGTATATGATGATATTGCCCTTGAAACT
CGGTGTAAGTTTGAGTCGAATAGACCTAGGGCTGTGCTCTTTGAAGGTCC
ACCAGGTACAGGGAAGACATCATGTGCTCGTGTTATTGCTAATCAAGCGG
GTGTTCCATTGATGTATGTGCCACTGGAGGTTGTCATGTCAAAATACTAC
GGTGAAAGTGAGCGGCTATTGGGGAAAGTGTTTTCTCTTGCCAATGAGCT
TCCAAACGGAGCTATTATTTTCCTGGACGAGGTTGATTCTTTTGCTGTTG
CTCGTGATAGTGAAATGCATGAAGCTACCCGAAGAATTTTGTCCGTGTTG
TTGAGACAGATTGATGGATTCGAACAGGATAAAAAGGTGGTGGTGATTGC
TGCAACAAATAGAAAGCAAGACCTTGATCCTGCTTTAATAAGTCGGTTTG
ATTCAATGATTACCTTTGGCCTACCGGATCATGAGAATCGTCAGGAAATA
GCAGCTCAGTATGCAAAGCACCTAACAAAAGCTGAACTGGCCGAATTAGC
CACTGCTACAGAGGAAATGTCGGGAAGAGATATTAGGGATGTGTGTCAAC
AAGCAGAGCGATCATGGGCATCAAAGATAATTCGAGGGCAAATCACTAAG
GATGGAGAACAGGCTTGTCTTCCACCTCTACAAGAATACATTGAGAGTGC
TACAAAC
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