CN188561

Overview
NameCN188561
Unique NameCN188561
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length707
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS8_SCHPO (26S protease regulatory subunit 8 homolog OS=Schizosaccharomyces pombe GN=let1 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 67/205 (32.68%), Postives = 101/205 (49.27%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEM----HEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKHXXXXXXXXXXXXXXXM---SGRDIRDVCQQA 605
            PE+++ + +         +P+ +L  GPPGTGKT  AR +A+      + V    ++ KY GE  R++ ++F +A E    +IIF+DE+DS   +R            +R +  LL Q+DGFE  K + VI ATNR   LDPAL+   R D  I F  P  E R EI   +++                M   SG +++ VC +A
Sbjct:  168 PELFESLGIP--------QPKGILLYGPPGTGKTLLARAVAHHTDCKFIRVSGSELVQKYIGEGSRMVRELFVMARE-HAPSIIFMDEIDSIGSSRSDSSGGSGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPSAEARAEILRIHSRSMNLTRGIDLKSIAEKMNGASGAELKGVCTEA 363          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_XENLA (26S protease regulatory subunit 7 OS=Xenopus laevis GN=psmc2 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 64/185 (34.59%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSE---MHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ VL  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       +IF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LDPAL+   R D  I F LPD E R  I   +A+                    +G +IR VC +A
Sbjct:  209 PKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEA 392          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_SCHPO (26S protease regulatory subunit 7 homolog OS=Schizosaccharomyces pombe GN=rpt1 PE=1 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 62/185 (33.51%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSE---MHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ ++  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       IIF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LD AL+   R D  + FGLPD E R  I   +AK                    +G ++R VC +A
Sbjct:  213 PKGIMLYGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACIIFFDEIDAIGGARFDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVLFATNRPNTLDEALMRPGRIDRKVEFGLPDLEGRAHILRIHAKSMAIDKDIRWELIARLCPSQTGAELRSVCTEA 396          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_MOUSE (26S protease regulatory subunit 7 OS=Mus musculus GN=Psmc2 PE=1 SV=5)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 64/185 (34.59%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSE---MHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ VL  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       +IF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LDPAL+   R D  I F LPD E R  I   +A+                    +G +IR VC +A
Sbjct:  209 PKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEA 392          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_MACFA (26S protease regulatory subunit 7 OS=Macaca fascicularis GN=PSMC2 PE=2 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 64/185 (34.59%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSE---MHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ VL  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       +IF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LDPAL+   R D  I F LPD E R  I   +A+                    +G +IR VC +A
Sbjct:  209 PKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEA 392          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_HUMAN (26S protease regulatory subunit 7 OS=Homo sapiens GN=PSMC2 PE=1 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 64/185 (34.59%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSE---MHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ VL  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       +IF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LDPAL+   R D  I F LPD E R  I   +A+                    +G +IR VC +A
Sbjct:  209 PKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEA 392          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS7_BOVIN (26S protease regulatory subunit 7 OS=Bos taurus GN=PSMC2 PE=2 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 64/185 (34.59%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSE---MHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ VL  GPPGTGKT CAR +AN+     + V    ++ KY GE  R++ ++F +A       +IF DE+D+   AR  +        +R +  L+ Q+DGF+    + V+ ATNR   LDPAL+   R D  I F LPD E R  I   +A+                    +G +IR VC +A
Sbjct:  209 PKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMA-RTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEA 392          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: YJNA_SCHPO (Uncharacterized AAA domain-containing protein C24B10.10c OS=Schizosaccharomyces pombe GN=SPCC24B10.10c PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.561e-20
Identity = 64/200 (32.00%), Postives = 101/200 (50.50%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDG-FEQDKKVVVIAATNRKQDLDPALISRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PEV+D       C      P+ +L  GPPG GKT  A+ +A Q+    + V + ++  K++GES +L+  +F+LA +L    IIF+DE+D+F   R    HEA  +I +  +   DG      +V+V+ ATNR  D+D A+  R   + +  LP+ E R++I   Y K                   +SG  I++VC+ A
Sbjct:  112 PEVFDTHGGLLSC------PKGLLLYGPPGCGKTMLAKALAKQSQATFINVSVGLLTDKWFGESNKLVDALFTLARKL-EPTIIFIDEIDTFLRQRQRTDHEAMAQIKAEFMSMWDGLLSGQSRVLVLGATNRPADIDEAIRRRMPKVFSIPLPNAEQRRKILELYLKKVPLEANFDWNGVVNATAGLSGSYIKEVCRSA 304          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: YHI5_SCHPO (Uncharacterized AAA domain-containing protein P22H7.05c OS=Schizosaccharomyces pombe GN=SPBP22H7.05c PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.561e-20
Identity = 63/149 (42.28%), Postives = 82/149 (55.03%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEV-----VMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEI 500
            PR VLF GPPGTGKT  ARV+A         +   +      +SK+ GE+ER L  +F  A  +   +IIF DE+D  A  R S+  +    I+S LL  +DG +   +VVVI ATNR  DLDPAL    RFD    F LP+ + R +I
Sbjct:  406 PRGVLFHGPPGTGKTLMARVLAANCSTKNQKISFFLRKGSDCLSKWVGEAERQLRLLFEEARRV-QPSIIFFDEIDGLAPIRSSKQEQTHSSIVSTLLALMDGLDTRGQVVVIGATNRPNDLDPALRRPGRFDREFYFPLPNKQARMKI 553          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: RIX7_YEAST (Ribosome biogenesis ATPase RIX7 OS=Saccharomyces cerevisiae GN=RIX7 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.561e-20
Identity = 56/170 (32.94%), Postives = 94/170 (55.29%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLAN-ELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAK 518
            PE+Y+ + +          P  VL  GPPG GKT  A+ +AN++    + +    +++KY GESER + +VF+ A   +P   +IF DE+D+    RD+ + E++ R+++ LL ++DG    + + VI ATNR   +DPA++   R D  +   LP+ E + +I     K
Sbjct:  556 PELYEKVGISA--------PGGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERSIRQVFTRARASVP--CVIFFDELDALVPRRDTSLSESSSRVVNTLLTELDGLNDRRGIFVIGATNRPDMIDPAMLRPGRLDKSLFIELPNTEEKLDIIKTLTK 715          

HSP 2 Score: 87.8113 bits (216), Expect = 6.140e-17
Identity = 61/172 (35.47%), Postives = 86/172 (50.00%), Query Frame = 3
Query:    6 LASSPEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARD-SEMHEATRRILSVLLRQIDGFEQD----KKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEI 500
            L   P ++ +I L T  +     PR VL  GPPG GKTS A  +A +  VP + +    V+S   GESE+ +  +F  A  L    ++F DE+D+    RD     E  RRI++ LL  +D    +    K V++I ATNR   LD AL    RFD  I   +P+  +R  I
Sbjct:  220 LIGLPILHPEIFLSTGVE----PPRGVLLHGPPGCGKTSIANALAGELQVPFISISAPSVVSGMSGESEKKIRDLFDEARSLA-PCLVFFDEIDAITPKRDGGAQREMERRIVAQLLTSMDELTMEKTNGKPVIIIGATNRPDSLDAALRRAGRFDREICLNVPNEVSRLHI 386          
The following BLAST results are available for this feature:
BLAST of CN188561 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 381
Match NameE-valueIdentityDescription
PRS8_SCHPO1.195e-2032.6826S protease regulatory subunit 8 homolog OS=Schiz... [more]
PRS7_XENLA1.195e-2034.5926S protease regulatory subunit 7 OS=Xenopus laevi... [more]
PRS7_SCHPO1.195e-2033.5126S protease regulatory subunit 7 homolog OS=Schiz... [more]
PRS7_MOUSE1.195e-2034.5926S protease regulatory subunit 7 OS=Mus musculus ... [more]
PRS7_MACFA1.195e-2034.5926S protease regulatory subunit 7 OS=Macaca fascic... [more]
PRS7_HUMAN1.195e-2034.5926S protease regulatory subunit 7 OS=Homo sapiens ... [more]
PRS7_BOVIN1.195e-2034.5926S protease regulatory subunit 7 OS=Bos taurus GN... [more]
YJNA_SCHPO1.561e-2032.00Uncharacterized AAA domain-containing protein C24B... [more]
YHI5_SCHPO1.561e-2042.28Uncharacterized AAA domain-containing protein P22H... [more]
RIX7_YEAST1.561e-2032.94Ribosome biogenesis ATPase RIX7 OS=Saccharomyces c... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006E21_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006E21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188561 ID=CN188561; Name=CN188561; organism=Citrus sinensis; type=EST; length=707bp
ACATTTTAGCTTCAAGCCCTGAAGTATATGATGATATTGCCCTTGAAACT
CGGTGTAAGTTTGAGTCGAATAGACCTAGGGCTGTGCTCTTTGAAGGTCC
ACCAGGTACAGGGAAGACATCATGTGCTCGTGTTATTGCTAATCAAGCGG
GTGTTCCATTGATGTATGTGCCACTGGAGGTTGTCATGTCAAAATACTAC
GGTGAAAGTGAGCGGCTATTGGGGAAAGTGTTTTCTCTTGCCAATGAGCT
TCCAAACGGAGCTATTATTTTCCTGGACGAGGTTGATTCTTTTGCTGTTG
CTCGTGATAGTGAAATGCATGAAGCTACCCGAAGAATTTTGTCCGTGTTG
TTGAGACAGATTGATGGATTCGAACAGGATAAAAAGGTGGTGGTGATTGC
TGCAACAAATAGAAAGCAAGACCTTGATCCTGCTTTAATAAGTCGGTTTG
ATTCAATGATTACCTTTGGCCTACCGGATCATGAGAATCGTCAGGAAATA
GCAGCTCAGTATGCAAAGCACCTAACAAAAGCTGAACTGGCCGAATTAGC
CACTGCTACAGAGGAAATGTCGGGAAGAGATATTAGGGATGTGTGTCAAC
AAGCAGAGCGATCATGGGCATCAAAGATAATTCGAGGGCAAATCACTAAG
GATGGAGAACAGGCTTGTCTTCCACCTCTACAAGAATACATTGAGAGTGC
TACAAAC
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