CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A3_OSCTI (Elongation factor 1-alpha 3 OS=Oscheius tipulae GN=eft-3 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.982e-106
Identity = 192/254 (75.59%), Postives = 213/254 (83.86%), Query Frame = 3
Query:   78 MGK-EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGK +KTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET K+Y T+IDAPGHRDFIKNMITGTSQADCAVL++   TG FEAGISK+GQTREHALLA TLGVKQMI  CNKMD+T P +S+ R+DEIV EV S+LKKVGYNP  +PFVPISGF GDNM+E S+N+ WYK            G TL++ALD I  P+RP+D+PLRLPLQDV
Sbjct:    1 MGKGDKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKFYVTIIDAPGHRDFIKNMITGTSQADCAVLVVACGTGEFEAGISKNGQTREHALLAQTLGVKQMIVACNKMDSTEPPFSEKRFDEIVTEVKSFLKKVGYNPATIPFVPISGFNGDNMLEPSSNMSWYKGWSVERKEGNASGKTLIEALDCIIPPQRPTDRPLRLPLQDV 254          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A3_XENLA (Elongation factor 1-alpha, oocyte form OS=Xenopus laevis PE=1 SV=2)

HSP 1 Score: 383.645 bits (984), Expect = 6.644e-106
Identity = 191/253 (75.49%), Postives = 213/253 (84.19%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+    G FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P +S+ R++EI KEVS+Y+KK+GYNP  VPFVPISG+ GDNM+E STN+ W+K            G TLL+ALD I  P+RP++KPLRLPLQDV
Sbjct:    1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETGKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAGGVGEFEAGISKNGQTREHALLAFTLGVKQLIIGVNKMDSTEPPFSQKRFEEITKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEASTNMPWFKGWKIERKEGNASGITLLEALDCIIPPQRPTNKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A3_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-3 PE=3 SV=1)

HSP 1 Score: 383.259 bits (983), Expect = 8.677e-106
Identity = 191/253 (75.49%), Postives = 213/253 (84.19%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD T  K+S+ RY+EIVKEVS ++KK+G+NP+ VPFVPISG+ GDNM++ STN+ W+K            G TLL+A+D I  P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAINKMDTT--KWSQDRYNEIVKEVSGFIKKIGFNPKSVPFVPISGWHGDNMLDESTNMPWFKGWNKETKAGSKTGKTLLEAIDAIEPPVRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_ORYLA (Elongation factor 1-alpha OS=Oryzias latipes GN=eef1a PE=2 SV=1)

HSP 1 Score: 382.874 bits (982), Expect = 1.133e-105
Identity = 191/253 (75.49%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HINIVVIGHVDSGKST+TGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P YS+AR++EI KEVS+Y+KK+GYNP  V FVPISG+ GDNM+E S  + W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKIHINIVVIGHVDSGKSTSTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSQARFEEIQKEVSTYIKKIGYNPAAVAFVPISGWHGDNMLEASDKMSWFKGWKIERKDGNASGTTLLEALDAILPPSRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_PICGU (Elongation factor 1-alpha OS=Pichia guilliermondii GN=TEF1 PE=3 SV=2)

HSP 1 Score: 382.489 bits (981), Expect = 1.480e-105
Identity = 190/253 (75.10%), Postives = 213/253 (84.19%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY+ TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLA+TLGV+Q+I   NKMD+   K+ K R++EI+KE S+++KKVGYNP+ VPFVPISG+ GDNMIE STN  WYK            G TLL+A+D I  P+RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAYTLGVRQLIVAVNKMDSV--KWDKNRFEEIIKETSNFVKKVGYNPKTVPFVPISGWNGDNMIEASTNCPWYKGWEKETKAGKSTGKTLLEAIDAIEPPQRPTDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_COCIM (Elongation factor 1-alpha OS=Coccidioides immitis GN=TEF PE=2 SV=2)

HSP 1 Score: 382.489 bits (981), Expect = 1.480e-105
Identity = 194/254 (76.38%), Postives = 216/254 (85.04%), Query Frame = 3
Query:   78 MGKE-KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKE KTHIN+VVIGHVDSGKSTTTGHLIYK GGID R IE+FEKEA E+ K+SFKYAWVLDKLKAERERGITIDIALWKFET KY+ TVIDAPGHRDFIKNMITGTSQADCA+LII + TG FEAGISKDGQTREHALLAFTLGVKQ+I   NKMD+T   +S+ R++EIVKEVS+++KKVGYNP+ VPFVPISGFEGDNMI+ STN  WYK            G TLLDA+D I+ P RP++KPLRLPLQDV
Sbjct:    1 MGKEEKTHINLVVIGHVDSGKSTTTGHLIYKCGGIDNRTIEKFEKEAEELGKKSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAFTLGVKQLIVAINKMDST--NWSEPRFNEIVKEVSNFIKKVGYNPKAVPFVPISGFEGDNMIQPSTNAPWYKGWNKETASGKHSGKTLLDAIDAIDPPTRPTEKPLRLPLQDV 252          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_CANAL (Elongation factor 1-alpha OS=Candida albicans GN=TEF1 PE=1 SV=1)

HSP 1 Score: 382.489 bits (981), Expect = 1.480e-105
Identity = 191/253 (75.49%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY+ TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD+   K+ K R++EI+KE S+++KKVGYNP+ VPFVPISG+ GDNMIE STN  WYK            G TLL+A+D I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDSV--KWDKNRFEEIIKETSNFVKKVGYNPKTVPFVPISGWNGDNMIEPSTNCPWYKGWEKETKSGKVTGKTLLEAIDAIEPPTRPTDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_SCHPO (Elongation factor 1-alpha-A OS=Schizosaccharomyces pombe GN=tef1a PE=1 SV=2)

HSP 1 Score: 381.719 bits (979), Expect = 2.525e-105
Identity = 191/253 (75.49%), Postives = 213/253 (84.19%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HIN+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA E+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCAVLII   TG FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD T   +S+AR++EIVKE S+++KKVG+NP+ VPFVP+SGF+GDNMIE +TN+ WY            KG TLL+A+D+I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_YARLI (Elongation factor 1-alpha OS=Yarrowia lipolytica GN=TEF PE=2 SV=2)

HSP 1 Score: 381.333 bits (978), Expect = 3.297e-105
Identity = 191/253 (75.49%), Postives = 211/253 (83.40%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVD+GKSTTTGHLIYK GGIDKR IE+FEKEA E+ K SFKYAWVLDKLKAERERGITIDIALWKF+T KYY TVIDAPGHRDFIKNMITGTSQADCA+LII    G FEAGISKDGQTREHALLAFTLGVKQ+I   NKMD+   K+S+ RY+EI KE ++++KKVGYNP+ VPFVPISG+ GDNMIE STN DWY            KG TLL+A+D I  P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKTHVNLVVIGHVDAGKSTTTGHLIYKCGGIDKRTIEKFEKEADELGKGSFKYAWVLDKLKAERERGITIDIALWKFQTPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVKQLIVAINKMDSV--KWSQDRYNEICKETANFVKKVGYNPKSVPFVPISGWNGDNMIEASTNCDWYKGWTKETKAGEVKGKTLLEAIDAIEPPVRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_ARTSA (Elongation factor 1-alpha OS=Artemia salina PE=1 SV=2)

HSP 1 Score: 381.333 bits (978), Expect = 3.297e-105
Identity = 191/253 (75.49%), Postives = 210/253 (83.00%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P +S+AR++EI KEVS+Y+KK+GYNP  V FVPISG+ GDNM+E S  L WYK            G TLLDALD I  P RP++KPLRLPLQDV
Sbjct:    1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPFSEARFEEIKKEVSAYIKKIGYNPAAVAFVPISGWHGDNMLEASDRLPWYKGWNIERKEGKADGKTLLDALDAILPPSRPTEKPLRLPLQDV 253          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A3_OSCTI2.982e-10675.59Elongation factor 1-alpha 3 OS=Oscheius tipulae GN... [more]
EF1A3_XENLA6.644e-10675.49Elongation factor 1-alpha, oocyte form OS=Xenopus ... [more]
EF1A3_RHIRA8.677e-10675.49Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A_ORYLA1.133e-10575.49Elongation factor 1-alpha OS=Oryzias latipes GN=ee... [more]
EF1A_PICGU1.480e-10575.10Elongation factor 1-alpha OS=Pichia guilliermondii... [more]
EF1A_COCIM1.480e-10576.38Elongation factor 1-alpha OS=Coccidioides immitis ... [more]
EF1A_CANAL1.480e-10575.49Elongation factor 1-alpha OS=Candida albicans GN=T... [more]
EF1A1_SCHPO2.525e-10575.49Elongation factor 1-alpha-A OS=Schizosaccharomyces... [more]
EF1A_YARLI3.297e-10575.49Elongation factor 1-alpha OS=Yarrowia lipolytica G... [more]
EF1A_ARTSA3.297e-10575.49Elongation factor 1-alpha OS=Artemia salina PE=1 S... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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