CV998065 | Citrus Genome Database

Overview


Name	CV998065
Unique Name	CV998065
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	476

Libraries


Library Name	Type
Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07-153	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig9802	contig	Csv1_Contig9802:656..1130.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI3_ORYSJ (Chitinase 3 OS=Oryza sativa subsp. japonica GN=Cht3 PE=2 SV=2)

HSP 1 Score: 106.301 bits (264), Expect = 1.544e-25
Identity = 46/78 (58.97%), Postives = 56/78 (71.79%), Query Frame = -1

Query:  198 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPN 431
            CH VI  +W PSA+D  A R+PGYG  TNIINGG+ECG G +  VA+RIGF+  +C   G+  GDNLDCYNQ PY P+
Sbjct:  243 CHAVITGQWTPSADDQAAGRVPGYGEITNIINGGVECGHGADDKVADRIGFYKRYCDMLGVSYGDNLDCYNQRPYPPS 320

HSP 2 Score: 30.8018 bits (68), Expect = 1.544e-25
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            A WFWMT Q PKPS
Sbjct:  229 AFWFWMTPQSPKPS 242

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI2_SOLTU (Endochitinase 2 (Fragment) OS=Solanum tuberosum GN=CHTB2 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.545e-25
Identity = 44/84 (52.38%), Postives = 60/84 (71.43%), Query Frame = -1

Query:  180 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 431
            CH+VI   W+PS  D  A+R+PG+G+ TNIINGG+ECG G +S V +RIGF+  +CG  G+ PGDNLDC NQ  +G  L+  ++
Sbjct:  233 CHDVITGRWQPSGTDQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRYCGILGVSPGDNLDCGNQRSFGNGLLVDTV 316

HSP 2 Score: 32.3426 bits (72), Expect = 1.545e-25
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q PKPS
Sbjct:  219 AIWFWMTPQSPKPS 232

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI5_PHAVU (Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.615e-25
Identity = 46/86 (53.49%), Postives = 60/86 (69.77%), Query Frame = -1

Query:  171 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM*SS 428
            H+VI   W PS+ DV A RLPGYG  TNIINGG+ECG+G +S V +RIGFF  +C   G+  G+NLDCY+Q P+G +L    + +S
Sbjct:  241 HDVITSRWTPSSADVAARRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFFKRYCDLLGVGYGNNLDCYSQTPFGNSLFLSDLVTS 326

HSP 2 Score: 33.4982 bits (75), Expect = 2.615e-25
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMTAQ PKPS
Sbjct:  226 ALWFWMTAQSPKPS 239

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI3_SOLTU (Endochitinase 3 (Fragment) OS=Solanum tuberosum GN=CHTB3 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.617e-25
Identity = 44/84 (52.38%), Postives = 60/84 (71.43%), Query Frame = -1

Query:  180 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 431
            CH+VI   W+PS  D  A+R+PG+G+ TNIINGG+ECG G +S V +RIGF+  +CG  G+ PGDNLDC NQ  +G  L+  ++
Sbjct:  235 CHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRYCGILGVSPGDNLDCGNQRSFGNGLLVDTV 318

HSP 2 Score: 32.3426 bits (72), Expect = 2.617e-25
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q PKPS
Sbjct:  221 AIWFWMTPQSPKPS 234

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 5.766e-25
Identity = 44/84 (52.38%), Postives = 61/84 (72.62%), Query Frame = -1

Query:  180 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 431
            CH+VII  W PS+ D  A+RLPG+G+ TNIINGG+ECG+G ++ V +RIGF+  +C   G+ PGDNLDC NQ  +G  L+  ++
Sbjct:  245 CHDVIIGRWNPSSADRAANRLPGFGVITNIINGGLECGRGTDNRVQDRIGFYRRYCSILGVTPGDNLDCVNQRWFGNALLVDTL 328

HSP 2 Score: 31.5722 bits (70), Expect = 5.766e-25
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMT Q PKPS
Sbjct:  231 ALWFWMTPQSPKPS 244

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIC_SOLLC (Basic 30 kDa endochitinase OS=Solanum lycopersicum GN=CHI9 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 5.768e-25
Identity = 44/84 (52.38%), Postives = 59/84 (70.24%), Query Frame = -1

Query:  180 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 431
            CH+VI   W+PS  D  A+R+PG+G+ TNIINGG+ECG G +S V +RIGF+  +CG  G+ PG+NLDC NQ  +G  L+   M
Sbjct:  239 CHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRYCGILGVSPGENLDCGNQRSFGNGLLVDIM 322

HSP 2 Score: 32.3426 bits (72), Expect = 5.768e-25
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q PKPS
Sbjct:  225 AIWFWMTPQSPKPS 238

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI9_ORYSJ (Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht9 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.501e-25
Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = -1

Query:  189 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMA 431
            CH+VI  +W PS+ D+ A R+PGYG+ TNIINGG+ECG G +  VANRIGF+  +C   GI  G NLDCY+Q P+   L A
Sbjct:  252 CHDVITGQWTPSSGDIAAGRVPGYGVITNIINGGLECGFGPDDRVANRIGFYQRYCDVLGIGYGSNLDCYDQRPFNSGLTA 332

HSP 2 Score: 31.5722 bits (70), Expect = 7.501e-25
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMT Q PKPS
Sbjct:  238 ALWFWMTPQSPKPS 251

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.271e-24
Identity = 42/72 (58.33%), Postives = 55/72 (76.39%), Query Frame = -1

Query:  216 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 431
            CH VI+ +W+PS  D  A R+PGYG+ TNIINGG+ECG+G ++ VA+RIGF+  +C   G+ PG NLDCYNQ
Sbjct:  237 CHAVIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVNPGGNLDCYNQ 308

HSP 2 Score: 32.3426 bits (72), Expect = 1.271e-24
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q PKPS
Sbjct:  223 AIWFWMTPQSPKPS 236

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIB_SOLLC (Acidic 27 kDa endochitinase OS=Solanum lycopersicum GN=CHI17 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.166e-24
Identity = 44/72 (61.11%), Postives = 54/72 (75.00%), Query Frame = -1

Query:  216 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 431
            CH+VI  +W PSA D +A+R PGYG+ TNIINGGIECGKG N  V +RIGF+  +C    + PGDNLDCY+Q
Sbjct:  170 CHDVITGQWTPSAADASANRQPGYGVITNIINGGIECGKGQNPQVEDRIGFYRRYCTILNVAPGDNLDCYDQ 241

HSP 2 Score: 30.4166 bits (67), Expect = 2.166e-24
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMTAQ  KPS
Sbjct:  156 AIWFWMTAQGNKPS 169

[Close]

BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIB_POPTR (Acidic endochitinase WIN6.2B OS=Populus trichocarpa PE=2 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 4.752e-24
Identity = 46/81 (56.79%), Postives = 55/81 (67.90%), Query Frame = -1

Query:  192 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGW-NSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLM 431
            CH VI + W PSA D  A R+PGYG+ TNIINGGIECG+G  N+   NRIGF+ T+C   G   G NLDCY Q P+G  L+
Sbjct:  217 CHAVITESWTPSAADSEAGRVPGYGVITNIINGGIECGQGGPNNANENRIGFYKTYCDSLGTTYGSNLDCYQQRPFGYGLL 297

HSP 2 Score: 31.5722 bits (70), Expect = 4.752e-24
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = -3

Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMT Q PKPS
Sbjct:  203 ALWFWMTPQSPKPS 216

The following BLAST results are available for this feature:

BLAST of CV998065 vs. ExPASy Swiss-Prot

BLAST of CV998065 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45


Match Name	E-value	Identity	Description
CHI3_ORYSJ	1.544e-25	58.97	Chitinase 3 OS=Oryza sativa subsp. japonica GN=Cht...	[more]
CHI2_SOLTU	1.545e-25	52.38	Endochitinase 2 (Fragment) OS=Solanum tuberosum GN...	[more]
CHI5_PHAVU	2.615e-25	53.49	Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1	[more]
CHI3_SOLTU	2.617e-25	52.38	Endochitinase 3 (Fragment) OS=Solanum tuberosum GN...	[more]
CHIT_SOLTU	5.766e-25	52.38	Endochitinase OS=Solanum tuberosum PE=2 SV=1	[more]
CHIC_SOLLC	5.768e-25	52.38	Basic 30 kDa endochitinase OS=Solanum lycopersicum...	[more]
CHI9_ORYSJ	7.501e-25	55.56	Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht...	[more]
CHI2_BRANA	1.271e-24	58.33	Endochitinase CH25 OS=Brassica napus PE=2 SV=1	[more]
CHIB_SOLLC	2.166e-24	61.11	Acidic 27 kDa endochitinase OS=Solanum lycopersicu...	[more]
CHIB_POPTR	4.752e-24	56.79	Acidic endochitinase WIN6.2B OS=Populus trichocarp...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS07_B-4_T7 Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07-153 Citrus sinensis cDNA clone TC58_B08_062, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV998065 ID=CV998065; Name=CV998065; organism=Citrus sinensis; type=EST; length=476bp
TTTTTTTTTTTTTTGGAGAGACTCATTGATCCATGTTGGATCAGTCTTAT
TTAATTTACCTACAATAAGAGCGTAACAAACTAACAATTTAGTTGTCCAC
CTACAAGTGTGACATCTAAAGTTTTTATTAAGTAGCTTTTATTAAGCTTA
TTTTACTAAAACGTTGATCATGATGATCACATAGATTGTGCCATCAAATT
AGGGCCATAAGGCCCTTGGTTGTAGCAATCAAGATTGTCGCCAGGCTGAA
TCCCAAACTTGCCACAGAAAGTGGTAAAGAACCCAATACGGTTAGCTACA
AAGCTATTCCAACCTTTGCCACATTCAATTCCACCGTTGATTATATTTGT
GGTTAGACCGTATCCCGGAAGCCGGCTGGCGTTTACGTCATTTGCTGATG
GTTTCCATTCGTCGATAATGACTTCGTGGCAACGATGGCTTTGGTGGCTG
TGCAGTCATCCAGAACCAGATTGCCT