CV998065

Overview
NameCV998065
Unique NameCV998065
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length476
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9802 contig Csv1_Contig9802:656..1130. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI1_ORYSJ (Chitinase 1 OS=Oryza sativa subsp. japonica GN=Cht1 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 6.175e-24
Identity = 42/75 (56.00%), Postives = 54/75 (72.00%), Query Frame = -1 
Query:  207 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 431
            CH V+  +W P+ ND  A R+PGYG+ TNIINGG+ECG G +S VA+RIGF+  +C   G+  G NLDCYNQ P+
Sbjct:  247 CHAVMTGQWTPNGNDQAAGRVPGYGVVTNIINGGVECGHGADSRVADRIGFYKRYCDMLGVSYGANLDCYNQRPF 321

HSP 2 Score: 30.8018 bits (68), Expect = 6.175e-24
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A WFWMT Q PKPS
Sbjct:  233 AFWFWMTPQSPKPS 246
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIQ_TOBAC (Acidic endochitinase Q OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.052e-23
Identity = 44/75 (58.67%), Postives = 55/75 (73.33%), Query Frame = -1 
Query:  204 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYG 428
            H+VII  W PSA D  A+R+PGYG+ TNIINGGIECG G N  V +RIG++  +CG   + PG+NLDCYNQ  +G
Sbjct:  177 HDVIIGRWTPSAADQAANRVPGYGVITNIINGGIECGIGRNDAVEDRIGYYRRYCGMLNVAPGENLDCYNQRNFG 251

HSP 2 Score: 28.8758 bits (63), Expect = 1.052e-23
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q  KPS
Sbjct:  162 AIWFWMTPQDNKPS 175
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI2_HORVU (26 kDa endochitinase 2 OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.367e-23
Identity = 41/74 (55.41%), Postives = 53/74 (71.62%), Query Frame = -1 
Query:  207 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 428
            H VI  +W PS  D  A R+PG+G+ TNIINGGIECG G +S VA+RIGF+  +C   G+  G+NLDCY+Q P+
Sbjct:  192 HAVIAGQWSPSGADRAAGRVPGFGVITNIINGGIECGHGQDSRVADRIGFYKRYCDILGVGYGNNLDCYSQRPF 265

HSP 2 Score: 37.3502 bits (85), Expect = 1.367e-23
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMTAQPPKPS
Sbjct:  177 AIWFWMTAQPPKPS 190
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.457e-23
Identity = 46/84 (54.76%), Postives = 62/84 (73.81%), Query Frame = -1 
Query:  180 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 431
            CH+VII  W+PS+ D  A+RLPG+G+ TNIINGG+ECG+G +S V +RIGF+  +C   G+ PGDNLDC NQ  +G  L+  +M
Sbjct:  241 CHDVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVSPGDNLDCGNQRSFGNGLLVDTM 324
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI2_ORYSJ (Chitinase 2 OS=Oryza sativa subsp. japonica GN=Cht2 PE=1 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.897e-23
Identity = 44/74 (59.46%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  207 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 428
            H+VI   W PS  D  A R PGYG+ TNI+NGG+ECG G +  VANRIGF+  +CG FGI  G NLDCYNQ P+
Sbjct:  256 HDVITGRWAPSPADAAAGRAPGYGVITNIVNGGLECGHGPDDRVANRIGFYQRYCGAFGIGTGGNLDCYNQRPF 329

HSP 2 Score: 27.7202 bits (60), Expect = 3.897e-23
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMT Q  KPS
Sbjct:  241 ALWFWMTPQGNKPS 254
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIC_SECCE (Basic endochitinase C OS=Secale cereale GN=rscc PE=1 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 5.099e-23
Identity = 42/74 (56.76%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  207 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 428
            H VI  +W PS  D  A R PG+G+ TNIINGG+ECG G +S VA+RIGF+  +C   G+  GDNLDCYNQ P+
Sbjct:  192 HAVITGKWSPSGADRAAGRAPGFGVITNIINGGLECGHGQDSRVADRIGFYKRYCDILGVGYGDNLDCYNQRPF 265

HSP 2 Score: 33.4982 bits (75), Expect = 5.099e-23
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMTAQ PKPS
Sbjct:  177 ALWFWMTAQAPKPS 190
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIT_PETHY (Acidic endochitinase OS=Petunia hybrida PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 8.642e-23
Identity = 43/72 (59.72%), Postives = 53/72 (73.61%), Query Frame = -1 
Query:  216 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 431
            CH+VI   W PSA D +A+R+PGYG+ TNIINGGIECGKG N+ V +RIG++        + PGDNLDCYNQ
Sbjct:  177 CHDVITGRWTPSAADTSANRVPGYGVITNIINGGIECGKGQNARVEDRIGYYRRNVSIMNVAPGDNLDCYNQ 248

HSP 2 Score: 28.4906 bits (62), Expect = 8.642e-23
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q  KPS
Sbjct:  163 AIWFWMTPQGNKPS 176
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIP_TOBAC (Acidic endochitinase P OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 8.645e-23
Identity = 43/71 (60.56%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  216 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 428
            H+VII  W PSA D +A+R PG G+ TNIINGGIECG G N+ V +RIG++  +CG   + PGDNLDCYNQ
Sbjct:  177 HDVIIGSWTPSAADQSANRAPGCGVITNIINGGIECGVGPNAAVEDRIGYYRRYCGMLNVAPGDNLDCYNQ 247

HSP 2 Score: 28.8758 bits (63), Expect = 8.645e-23
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q  KPS
Sbjct:  162 AIWFWMTPQDNKPS 175
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHID_SOLLC (Basic endochitinase (Fragment) OS=Solanum lycopersicum GN=CHI14 PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.126e-22
Identity = 39/75 (52.00%), Postives = 51/75 (68.00%), Query Frame = -1 
Query:  204 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYG 428
            H+VI   W PS  D    R+PG+G+ TNIINGG+EC  G N+ + NRIGF+  +C   G+ PG+NLDC NQ P+G
Sbjct:  172 HDVITGRWSPSVADSAPGRVPGFGVITNIINGGMECNSGSNALMDNRIGFYRRYCQILGVDPGNNLDCANQRPFG 246

HSP 2 Score: 33.4982 bits (75), Expect = 1.126e-22
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMTAQ PKPS
Sbjct:  157 ALWFWMTAQQPKPS 170
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI1_HORVU (26 kDa endochitinase 1 OS=Hordeum vulgare PE=2 SV=4)

HSP 1 Score: 93.5893 bits (231), Expect = 1.894e-22
Identity = 40/74 (54.05%), Postives = 53/74 (71.62%), Query Frame = -1 
Query:  207 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 428
            H VI  +W PS  D  A R+PG+G+ TNI+NGGIECG G +S VA+RIGF+  +C   G+  G+NLDCY+Q P+
Sbjct:  244 HAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILGVGYGNNLDCYSQRPF 317

HSP 2 Score: 33.113 bits (74), Expect = 1.894e-22
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMTAQ PKPS
Sbjct:  229 AMWFWMTAQAPKPS 242
The following BLAST results are available for this feature:
BLAST of CV998065 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
CHI1_ORYSJ6.175e-2456.00Chitinase 1 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIQ_TOBAC1.052e-2358.67Acidic endochitinase Q OS=Nicotiana tabacum PE=1 S... [more]
CHI2_HORVU1.367e-2355.4126 kDa endochitinase 2 OS=Hordeum vulgare PE=1 SV=... [more]
CHI2_TOBAC2.457e-2354.76Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1... [more]
CHI2_ORYSJ3.897e-2359.46Chitinase 2 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIC_SECCE5.099e-2356.76Basic endochitinase C OS=Secale cereale GN=rscc PE... [more]
CHIT_PETHY8.642e-2359.72Acidic endochitinase OS=Petunia hybrida PE=2 SV=1[more]
CHIP_TOBAC8.645e-2360.56Acidic endochitinase P OS=Nicotiana tabacum PE=1 S... [more]
CHID_SOLLC1.126e-2252.00Basic endochitinase (Fragment) OS=Solanum lycopers... [more]
CHI1_HORVU1.894e-2254.0526 kDa endochitinase 1 OS=Hordeum vulgare PE=2 SV=... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS07_B-4_T7 Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07-153 Citrus sinensis cDNA clone TC58_B08_062, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV998065 ID=CV998065; Name=CV998065; organism=Citrus sinensis; type=EST; length=476bp
TTTTTTTTTTTTTTGGAGAGACTCATTGATCCATGTTGGATCAGTCTTAT
TTAATTTACCTACAATAAGAGCGTAACAAACTAACAATTTAGTTGTCCAC
CTACAAGTGTGACATCTAAAGTTTTTATTAAGTAGCTTTTATTAAGCTTA
TTTTACTAAAACGTTGATCATGATGATCACATAGATTGTGCCATCAAATT
AGGGCCATAAGGCCCTTGGTTGTAGCAATCAAGATTGTCGCCAGGCTGAA
TCCCAAACTTGCCACAGAAAGTGGTAAAGAACCCAATACGGTTAGCTACA
AAGCTATTCCAACCTTTGCCACATTCAATTCCACCGTTGATTATATTTGT
GGTTAGACCGTATCCCGGAAGCCGGCTGGCGTTTACGTCATTTGCTGATG
GTTTCCATTCGTCGATAATGACTTCGTGGCAACGATGGCTTTGGTGGCTG
TGCAGTCATCCAGAACCAGATTGCCT
back to top