CV998065

Overview
NameCV998065
Unique NameCV998065
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length476
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9802 contig Csv1_Contig9802:656..1130. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI11_ORYSJ (Chitinase 11 OS=Oryza sativa subsp. japonica GN=Cht11 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.904e-22
Identity = 39/77 (50.65%), Postives = 57/77 (74.03%), Query Frame = -1 
Query:  204 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVA-NRIGFFTTFCGKFGIQPGDNLDCYNQGPYG 431
            CH+VI ++W PS +D ++ RLPGYG+ TNIINGG ECGKG+++  A +R+G++  +C  F +  GDN+ C +Q PYG
Sbjct:  178 CHDVITNQWTPSGDDRSSGRLPGYGMATNIINGGEECGKGYSTDNAKDRVGYYKRYCDMFRVGYGDNIACRDQKPYG 254

HSP 2 Score: 34.2686 bits (77), Expect = 1.904e-22
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMTAQ PKPS
Sbjct:  164 AIWFWMTAQSPKPS 177
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI12_ORYSJ (Chitinase 12 OS=Oryza sativa subsp. japonica GN=Cht12 PE=2 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 2.463e-22
Identity = 41/76 (53.95%), Postives = 53/76 (69.74%), Query Frame = -1 
Query:  204 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYG 431
            CH V   +W PSA+D  A R+PGYG+ TNIINGG+ECG G +  VA+RIGF+  +C   G+    NLDCY+Q P+G
Sbjct:  250 CHAVATGQWTPSADDQAAGRVPGYGVITNIINGGLECGHGEDDRVADRIGFYKRYCDILGVSYDANLDCYSQRPFG 325

HSP 2 Score: 30.8018 bits (68), Expect = 2.463e-22
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A WFWMT Q PKPS
Sbjct:  236 AFWFWMTPQSPKPS 249
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI6_POPTR (Acidic endochitinase WIN6 OS=Populus trichocarpa GN=WIN6 PE=2 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 4.165e-22
Identity = 44/80 (55.00%), Postives = 53/80 (66.25%), Query Frame = -1 
Query:  195 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGW-NSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNL 431
            CH VI   W PSA D+ A R+PGYG+ TNIINGGIECG+G  N+   +RIGF+  +C   G   G NLDCY Q P+G  L
Sbjct:  254 CHAVITGNWTPSAADLEAGRVPGYGVITNIINGGIECGQGGPNAANEDRIGFYKKYCDSLGTTYGSNLDCYQQRPFGYGL 333

HSP 2 Score: 30.0314 bits (66), Expect = 4.165e-22
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWM  Q PKPS
Sbjct:  240 AIWFWMKPQSPKPS 253
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIA_SECCE (Basic endochitinase A OS=Secale cereale GN=rsca PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 4.170e-22
Identity = 40/74 (54.05%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  207 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 428
            H VI  +W PS  D  A R+PG+G+ TNI+NGGIECG G +S VA+RIGF+  +C    +  G+NLDCYNQ P+
Sbjct:  247 HAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILRVGYGNNLDCYNQRPF 320

HSP 2 Score: 33.113 bits (74), Expect = 4.170e-22
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMTAQ PKPS
Sbjct:  232 AMWFWMTAQAPKPS 245
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIA_SOLCI (Acidic endochitinase pcht28 OS=Solanum chilense PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 4.194e-22
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = -1 
Query:  216 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 431
            CH VII +W PS  D  A+R+PGYG+ TNIINGG+EC  G N+ V +RIGF+  +CG   +  G+NLDC NQ
Sbjct:  176 CHNVIIGQWTPSPADTAANRVPGYGVITNIINGGLECNMGPNTAVESRIGFYRRYCGMLNVPTGENLDCNNQ 247

HSP 2 Score: 30.8018 bits (68), Expect = 4.194e-22
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMTAQ  KPS
Sbjct:  162 AIWFWMTAQDNKPS 175
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 5.428e-22
Identity = 42/84 (50.00%), Postives = 55/84 (65.48%), Query Frame = -1 
Query:  180 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 431
            CH VI   W PS  D +A RLPG+G+ TNIINGG+ECGKG    V +RIGF+  +C    +  G+NLDC NQ P+G  L+  ++
Sbjct:  231 CHNVITGGWTPSGADRSAGRLPGFGVITNIINGGVECGKGVVPQVQDRIGFYKRYCDILRVSYGNNLDCNNQRPFGSGLLLDTI 314

HSP 2 Score: 30.8018 bits (68), Expect = 5.428e-22
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A WFWMT Q PKPS
Sbjct:  217 AFWFWMTPQSPKPS 230
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHI8_ORYSJ (Chitinase 8 OS=Oryza sativa subsp. japonica GN=Cht8 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.452e-22
Identity = 41/72 (56.94%), Postives = 49/72 (68.06%), Query Frame = -1 
Query:  216 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 431
            CH+VI+  W PSA D  A R+PGYG+ TNIINGGIECG G N    +RIG++  +C   G   G NLDCYNQ
Sbjct:  185 CHDVILGRWTPSAADTAAGRVPGYGVITNIINGGIECGVGQNDANVDRIGYYKRYCDMLGAGYGSNLDCYNQ 256

HSP 2 Score: 30.4166 bits (67), Expect = 5.452e-22
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMTAQ  KPS
Sbjct:  171 AIWFWMTAQGNKPS 184
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIX_PEA (Endochitinase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.195e-21
Identity = 41/71 (57.75%), Postives = 52/71 (73.24%), Query Frame = -1 
Query:  216 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 428
            H+VI   W PSA D +A R+PGYG+ TNIINGGIECG G ++ V +R+GF+  +C  FG+ PG NLDC NQ
Sbjct:  246 HDVITGRWTPSAADSSAGRVPGYGVITNIINGGIECGHGQDNRVDDRVGFYKRYCQIFGVDPGGNLDCNNQ 316

HSP 2 Score: 28.8758 bits (63), Expect = 1.195e-21
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q  KPS
Sbjct:  231 AIWFWMTPQANKPS 244
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIA_SOLLC (Acidic 26 kDa endochitinase OS=Solanum lycopersicum GN=CHI3 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.578e-21
Identity = 39/72 (54.17%), Postives = 49/72 (68.06%), Query Frame = -1 
Query:  216 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 431
            CH VII +W PS  D  A+R+PGYG+ TNIING  ECG G N+   +RIGF+  +CG   +  G+NLDC NQ
Sbjct:  176 CHNVIIGQWTPSPKDTAANRVPGYGVITNIINGQFECGMGPNTAAESRIGFYRRYCGMLNVPTGENLDCNNQ 247

HSP 2 Score: 28.8758 bits (63), Expect = 7.578e-21
Identity = 11/14 (78.57%), Postives = 11/14 (78.57%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            AIWFWMT Q  KPS
Sbjct:  162 AIWFWMTEQDNKPS 175
[Close]
BLAST of CV998065 vs. ExPASy Swiss-Prot
Match: CHIT_AVESA (Endochitinase (Fragments) OS=Avena sativa PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.328e-19
Identity = 39/74 (52.70%), Postives = 49/74 (66.22%), Query Frame = -1 
Query:  207 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 428
            H+VI   W PS+ D  A R+PGYG+ TNII+GG+ECGKG  S VA+RIG++            DNLDCYNQ P+
Sbjct:  137 HDVITGRWSPSSTDKAAGRVPGYGVLTNIIDGGVECGKGQESHVADRIGYYK-----------DNLDCYNQKPF 199

HSP 2 Score: 31.5722 bits (70), Expect = 2.328e-19
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = -3 
Query:  433 AIWFWMTAQPPKPS 474
            A+WFWMT Q PKPS
Sbjct:  122 ALWFWMTPQSPKPS 135
The following BLAST results are available for this feature:
BLAST of CV998065 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
CHI11_ORYSJ1.904e-2250.65Chitinase 11 OS=Oryza sativa subsp. japonica GN=Ch... [more]
CHI12_ORYSJ2.463e-2253.95Chitinase 12 OS=Oryza sativa subsp. japonica GN=Ch... [more]
CHI6_POPTR4.165e-2255.00Acidic endochitinase WIN6 OS=Populus trichocarpa G... [more]
CHIA_SECCE4.170e-2254.05Basic endochitinase A OS=Secale cereale GN=rsca PE... [more]
CHIA_SOLCI4.194e-2255.56Acidic endochitinase pcht28 OS=Solanum chilense PE... [more]
CHIB_VITVI5.428e-2250.00Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE... [more]
CHI8_ORYSJ5.452e-2256.94Chitinase 8 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIX_PEA1.195e-2157.75Endochitinase OS=Pisum sativum PE=2 SV=1[more]
CHIA_SOLLC7.578e-2154.17Acidic 26 kDa endochitinase OS=Solanum lycopersicu... [more]
CHIT_AVESA2.328e-1952.70Endochitinase (Fragments) OS=Avena sativa PE=1 SV=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS07_B-4_T7 Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07-153 Citrus sinensis cDNA clone TC58_B08_062, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV998065 ID=CV998065; Name=CV998065; organism=Citrus sinensis; type=EST; length=476bp
TTTTTTTTTTTTTTGGAGAGACTCATTGATCCATGTTGGATCAGTCTTAT
TTAATTTACCTACAATAAGAGCGTAACAAACTAACAATTTAGTTGTCCAC
CTACAAGTGTGACATCTAAAGTTTTTATTAAGTAGCTTTTATTAAGCTTA
TTTTACTAAAACGTTGATCATGATGATCACATAGATTGTGCCATCAAATT
AGGGCCATAAGGCCCTTGGTTGTAGCAATCAAGATTGTCGCCAGGCTGAA
TCCCAAACTTGCCACAGAAAGTGGTAAAGAACCCAATACGGTTAGCTACA
AAGCTATTCCAACCTTTGCCACATTCAATTCCACCGTTGATTATATTTGT
GGTTAGACCGTATCCCGGAAGCCGGCTGGCGTTTACGTCATTTGCTGATG
GTTTCCATTCGTCGATAATGACTTCGTGGCAACGATGGCTTTGGTGGCTG
TGCAGTCATCCAGAACCAGATTGCCT
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