CV998064

Overview
NameCV998064
Unique NameCV998064
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length476
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12923 contig Csv1_Contig12923:623..1097. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI3_ORYSJ (Chitinase 3 OS=Oryza sativa subsp. japonica GN=Cht3 PE=2 SV=2)

HSP 1 Score: 106.301 bits (264), Expect = 4.432e-25
Identity = 46/78 (58.97%), Postives = 56/78 (71.79%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPN 276
            CH VI  +W PSA+D  A R+PGYG  TNIINGG+ECG G +  VA+RIGF+  +C   G+  GDNLDCYNQ PY P+
Sbjct:  243 CHAVITGQWTPSADDQAAGRVPGYGEITNIINGGVECGHGADDKVADRIGFYKRYCDMLGVSYGDNLDCYNQRPYPPS 320

HSP 2 Score: 29.261 bits (64), Expect = 4.432e-25
Identity = 10/12 (83.33%), Postives = 10/12 (83.33%), Query Frame = 3 
Query:    6 WFWMTAQPPKPS 41
            WFWMT Q PKPS
Sbjct:  231 WFWMTPQSPKPS 242
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI2_SOLTU (Endochitinase 2 (Fragment) OS=Solanum tuberosum GN=CHTB2 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.434e-25
Identity = 44/84 (52.38%), Postives = 60/84 (71.43%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 294
            CH+VI   W+PS  D  A+R+PG+G+ TNIINGG+ECG G +S V +RIGF+  +CG  G+ PGDNLDC NQ  +G  L+  ++
Sbjct:  233 CHDVITGRWQPSGTDQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRYCGILGVSPGDNLDCGNQRSFGNGLLVDTV 316

HSP 2 Score: 30.8018 bits (68), Expect = 4.434e-25
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMT Q PKPS
Sbjct:  220 IWFWMTPQSPKPS 232
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI5_PHAVU (Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 7.504e-25
Identity = 46/86 (53.49%), Postives = 60/86 (69.77%), Query Frame = 1 
Query:   46 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM*SS 303
            H+VI   W PS+ DV A RLPGYG  TNIINGG+ECG+G +S V +RIGFF  +C   G+  G+NLDCY+Q P+G +L    + +S
Sbjct:  241 HDVITSRWTPSSADVAARRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFFKRYCDLLGVGYGNNLDCYSQTPFGNSLFLSDLVTS 326

HSP 2 Score: 31.9574 bits (71), Expect = 7.504e-25
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            +WFWMTAQ PKPS
Sbjct:  227 LWFWMTAQSPKPS 239
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI3_SOLTU (Endochitinase 3 (Fragment) OS=Solanum tuberosum GN=CHTB3 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 7.508e-25
Identity = 44/84 (52.38%), Postives = 60/84 (71.43%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 294
            CH+VI   W+PS  D  A+R+PG+G+ TNIINGG+ECG G +S V +RIGF+  +CG  G+ PGDNLDC NQ  +G  L+  ++
Sbjct:  235 CHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRYCGILGVSPGDNLDCGNQRSFGNGLLVDTV 318

HSP 2 Score: 30.8018 bits (68), Expect = 7.508e-25
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMT Q PKPS
Sbjct:  222 IWFWMTPQSPKPS 234
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.654e-24
Identity = 44/84 (52.38%), Postives = 61/84 (72.62%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 294
            CH+VII  W PS+ D  A+RLPG+G+ TNIINGG+ECG+G ++ V +RIGF+  +C   G+ PGDNLDC NQ  +G  L+  ++
Sbjct:  245 CHDVIIGRWNPSSADRAANRLPGFGVITNIINGGLECGRGTDNRVQDRIGFYRRYCSILGVTPGDNLDCVNQRWFGNALLVDTL 328

HSP 2 Score: 30.0314 bits (66), Expect = 1.654e-24
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            +WFWMT Q PKPS
Sbjct:  232 LWFWMTPQSPKPS 244
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIC_SOLLC (Basic 30 kDa endochitinase OS=Solanum lycopersicum GN=CHI9 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.655e-24
Identity = 44/84 (52.38%), Postives = 59/84 (70.24%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 294
            CH+VI   W+PS  D  A+R+PG+G+ TNIINGG+ECG G +S V +RIGF+  +CG  G+ PG+NLDC NQ  +G  L+   M
Sbjct:  239 CHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRYCGILGVSPGENLDCGNQRSFGNGLLVDIM 322

HSP 2 Score: 30.8018 bits (68), Expect = 1.655e-24
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMT Q PKPS
Sbjct:  226 IWFWMTPQSPKPS 238
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI9_ORYSJ (Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht9 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.152e-24
Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMA 285
            CH+VI  +W PS+ D+ A R+PGYG+ TNIINGG+ECG G +  VANRIGF+  +C   GI  G NLDCY+Q P+   L A
Sbjct:  252 CHDVITGQWTPSSGDIAAGRVPGYGVITNIINGGLECGFGPDDRVANRIGFYQRYCDVLGIGYGSNLDCYDQRPFNSGLTA 332

HSP 2 Score: 30.0314 bits (66), Expect = 2.152e-24
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            +WFWMT Q PKPS
Sbjct:  239 LWFWMTPQSPKPS 251
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 3.647e-24
Identity = 42/72 (58.33%), Postives = 55/72 (76.39%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 258
            CH VI+ +W+PS  D  A R+PGYG+ TNIINGG+ECG+G ++ VA+RIGF+  +C   G+ PG NLDCYNQ
Sbjct:  237 CHAVIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVNPGGNLDCYNQ 308

HSP 2 Score: 30.8018 bits (68), Expect = 3.647e-24
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMT Q PKPS
Sbjct:  224 IWFWMTPQSPKPS 236
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIB_SOLLC (Acidic 27 kDa endochitinase OS=Solanum lycopersicum GN=CHI17 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 6.214e-24
Identity = 44/72 (61.11%), Postives = 54/72 (75.00%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 258
            CH+VI  +W PSA D +A+R PGYG+ TNIINGGIECGKG N  V +RIGF+  +C    + PGDNLDCY+Q
Sbjct:  170 CHDVITGQWTPSAADASANRQPGYGVITNIINGGIECGKGQNPQVEDRIGFYRRYCTILNVAPGDNLDCYDQ 241

HSP 2 Score: 28.8758 bits (63), Expect = 6.214e-24
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMTAQ  KPS
Sbjct:  157 IWFWMTAQGNKPS 169
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIB_POPTR (Acidic endochitinase WIN6.2B OS=Populus trichocarpa PE=2 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 1.363e-23
Identity = 46/81 (56.79%), Postives = 55/81 (67.90%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGW-NSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLM 282
            CH VI + W PSA D  A R+PGYG+ TNIINGGIECG+G  N+   NRIGF+ T+C   G   G NLDCY Q P+G  L+
Sbjct:  217 CHAVITESWTPSAADSEAGRVPGYGVITNIINGGIECGQGGPNNANENRIGFYKTYCDSLGTTYGSNLDCYQQRPFGYGLL 297

HSP 2 Score: 30.0314 bits (66), Expect = 1.363e-23
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            +WFWMT Q PKPS
Sbjct:  204 LWFWMTPQSPKPS 216
The following BLAST results are available for this feature:
BLAST of CV998064 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
CHI3_ORYSJ4.432e-2558.97Chitinase 3 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHI2_SOLTU4.434e-2552.38Endochitinase 2 (Fragment) OS=Solanum tuberosum GN... [more]
CHI5_PHAVU7.504e-2553.49Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1[more]
CHI3_SOLTU7.508e-2552.38Endochitinase 3 (Fragment) OS=Solanum tuberosum GN... [more]
CHIT_SOLTU1.654e-2452.38Endochitinase OS=Solanum tuberosum PE=2 SV=1[more]
CHIC_SOLLC1.655e-2452.38Basic 30 kDa endochitinase OS=Solanum lycopersicum... [more]
CHI9_ORYSJ2.152e-2455.56Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHI2_BRANA3.647e-2458.33Endochitinase CH25 OS=Brassica napus PE=2 SV=1[more]
CHIB_SOLLC6.214e-2461.11Acidic 27 kDa endochitinase OS=Solanum lycopersicu... [more]
CHIB_POPTR1.363e-2356.79Acidic endochitinase WIN6.2B OS=Populus trichocarp... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS07_B-4_T3 Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07-153 Citrus sinensis cDNA clone TC28_D04_026, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV998064 ID=CV998064; Name=CV998064; organism=Citrus sinensis; type=EST; length=476bp
CAATCTGGTTCTGGATGACTGCACAGCCACCAAAGCCATCGTTGCCACGA
AGTCATTATCGACGAATGGAAACCATCAGCAAATGACGTAAACGCCAGCC
GGCTTCCGGGATACGGTCTAACCACAAATATAATCAACGGTGGAATTGAA
TGTGGCAAAGGTTGGAATAGCTTTGTAGCTAACCGTATTGGGTTCTTTAC
CACTTTCTGTGGCAAGTTTGGGATTCAGCCTGGCGACAATCTTGATTGCT
ACAACCAAGGGCCTTATGGCCCTAATTTGATGGCACAATCTATGTGATCA
TCATGATCAACGTTTTAGTAAAATAAGCTTAATAAAAGCTACTTAATAAA
AACTTTAGATGTCACACTTGTAGGTGGACAACTAAATTGTTAGTTTGTTA
CGCTCTTATTGTAGGTAAATTAAATAAGACTGATCCAACATGGATCAATG
AGTCTTCTCCAAAAAAAAAAAAAAAA
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