CV998064

Overview
NameCV998064
Unique NameCV998064
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length476
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12923 contig Csv1_Contig12923:623..1097. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI11_ORYSJ (Chitinase 11 OS=Oryza sativa subsp. japonica GN=Cht11 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 5.456e-22
Identity = 39/77 (50.65%), Postives = 57/77 (74.03%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVA-NRIGFFTTFCGKFGIQPGDNLDCYNQGPYG 270
            CH+VI ++W PS +D ++ RLPGYG+ TNIINGG ECGKG+++  A +R+G++  +C  F +  GDN+ C +Q PYG
Sbjct:  178 CHDVITNQWTPSGDDRSSGRLPGYGMATNIINGGEECGKGYSTDNAKDRVGYYKRYCDMFRVGYGDNIACRDQKPYG 254

HSP 2 Score: 32.7278 bits (73), Expect = 5.456e-22
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMTAQ PKPS
Sbjct:  165 IWFWMTAQSPKPS 177
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI12_ORYSJ (Chitinase 12 OS=Oryza sativa subsp. japonica GN=Cht12 PE=2 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 7.056e-22
Identity = 41/76 (53.95%), Postives = 53/76 (69.74%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYG 270
            CH V   +W PSA+D  A R+PGYG+ TNIINGG+ECG G +  VA+RIGF+  +C   G+    NLDCY+Q P+G
Sbjct:  250 CHAVATGQWTPSADDQAAGRVPGYGVITNIINGGLECGHGEDDRVADRIGFYKRYCDILGVSYDANLDCYSQRPFG 325

HSP 2 Score: 29.261 bits (64), Expect = 7.056e-22
Identity = 10/12 (83.33%), Postives = 10/12 (83.33%), Query Frame = 3 
Query:    6 WFWMTAQPPKPS 41
            WFWMT Q PKPS
Sbjct:  238 WFWMTPQSPKPS 249
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI6_POPTR (Acidic endochitinase WIN6 OS=Populus trichocarpa GN=WIN6 PE=2 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 1.193e-21
Identity = 44/80 (55.00%), Postives = 53/80 (66.25%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGW-NSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNL 279
            CH VI   W PSA D+ A R+PGYG+ TNIINGGIECG+G  N+   +RIGF+  +C   G   G NLDCY Q P+G  L
Sbjct:  254 CHAVITGNWTPSAADLEAGRVPGYGVITNIINGGIECGQGGPNAANEDRIGFYKKYCDSLGTTYGSNLDCYQQRPFGYGL 333

HSP 2 Score: 28.4906 bits (62), Expect = 1.193e-21
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWM  Q PKPS
Sbjct:  241 IWFWMKPQSPKPS 253
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIA_SECCE (Basic endochitinase A OS=Secale cereale GN=rsca PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.195e-21
Identity = 40/74 (54.05%), Postives = 52/74 (70.27%), Query Frame = 1 
Query:   46 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 267
            H VI  +W PS  D  A R+PG+G+ TNI+NGGIECG G +S VA+RIGF+  +C    +  G+NLDCYNQ P+
Sbjct:  247 HAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILRVGYGNNLDCYNQRPF 320

HSP 2 Score: 31.5722 bits (70), Expect = 1.195e-21
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            +WFWMTAQ PKPS
Sbjct:  233 MWFWMTAQAPKPS 245
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIA_SOLCI (Acidic endochitinase pcht28 OS=Solanum chilense PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.202e-21
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 258
            CH VII +W PS  D  A+R+PGYG+ TNIINGG+EC  G N+ V +RIGF+  +CG   +  G+NLDC NQ
Sbjct:  176 CHNVIIGQWTPSPADTAANRVPGYGVITNIINGGLECNMGPNTAVESRIGFYRRYCGMLNVPTGENLDCNNQ 247

HSP 2 Score: 29.261 bits (64), Expect = 1.202e-21
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMTAQ  KPS
Sbjct:  163 IWFWMTAQDNKPS 175
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.555e-21
Identity = 42/84 (50.00%), Postives = 55/84 (65.48%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPYGPNLMAQSM 294
            CH VI   W PS  D +A RLPG+G+ TNIINGG+ECGKG    V +RIGF+  +C    +  G+NLDC NQ P+G  L+  ++
Sbjct:  231 CHNVITGGWTPSGADRSAGRLPGFGVITNIINGGVECGKGVVPQVQDRIGFYKRYCDILRVSYGNNLDCNNQRPFGSGLLLDTI 314

HSP 2 Score: 29.261 bits (64), Expect = 1.555e-21
Identity = 10/12 (83.33%), Postives = 10/12 (83.33%), Query Frame = 3 
Query:    6 WFWMTAQPPKPS 41
            WFWMT Q PKPS
Sbjct:  219 WFWMTPQSPKPS 230
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHI8_ORYSJ (Chitinase 8 OS=Oryza sativa subsp. japonica GN=Cht8 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.562e-21
Identity = 41/72 (56.94%), Postives = 49/72 (68.06%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 258
            CH+VI+  W PSA D  A R+PGYG+ TNIINGGIECG G N    +RIG++  +C   G   G NLDCYNQ
Sbjct:  185 CHDVILGRWTPSAADTAAGRVPGYGVITNIINGGIECGVGQNDANVDRIGYYKRYCDMLGAGYGSNLDCYNQ 256

HSP 2 Score: 28.8758 bits (63), Expect = 1.562e-21
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMTAQ  KPS
Sbjct:  172 IWFWMTAQGNKPS 184
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIX_PEA (Endochitinase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 3.422e-21
Identity = 41/71 (57.75%), Postives = 52/71 (73.24%), Query Frame = 1 
Query:   46 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 258
            H+VI   W PSA D +A R+PGYG+ TNIINGGIECG G ++ V +R+GF+  +C  FG+ PG NLDC NQ
Sbjct:  246 HDVITGRWTPSAADSSAGRVPGYGVITNIINGGIECGHGQDNRVDDRVGFYKRYCQIFGVDPGGNLDCNNQ 316

HSP 2 Score: 27.335 bits (59), Expect = 3.422e-21
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMT Q  KPS
Sbjct:  232 IWFWMTPQANKPS 244
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIA_SOLLC (Acidic 26 kDa endochitinase OS=Solanum lycopersicum GN=CHI3 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.170e-20
Identity = 39/72 (54.17%), Postives = 49/72 (68.06%), Query Frame = 1 
Query:   43 CHEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQ 258
            CH VII +W PS  D  A+R+PGYG+ TNIING  ECG G N+   +RIGF+  +CG   +  G+NLDC NQ
Sbjct:  176 CHNVIIGQWTPSPKDTAANRVPGYGVITNIINGQFECGMGPNTAAESRIGFYRRYCGMLNVPTGENLDCNNQ 247

HSP 2 Score: 27.335 bits (59), Expect = 2.170e-20
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            IWFWMT Q  KPS
Sbjct:  163 IWFWMTEQDNKPS 175
[Close]
BLAST of CV998064 vs. ExPASy Swiss-Prot
Match: CHIT_AVESA (Endochitinase (Fragments) OS=Avena sativa PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 6.662e-19
Identity = 39/74 (52.70%), Postives = 49/74 (66.22%), Query Frame = 1 
Query:   46 HEVIIDEWKPSANDVNASRLPGYGLTTNIINGGIECGKGWNSFVANRIGFFTTFCGKFGIQPGDNLDCYNQGPY 267
            H+VI   W PS+ D  A R+PGYG+ TNII+GG+ECGKG  S VA+RIG++            DNLDCYNQ P+
Sbjct:  137 HDVITGRWSPSSTDKAAGRVPGYGVLTNIIDGGVECGKGQESHVADRIGYYK-----------DNLDCYNQKPF 199

HSP 2 Score: 30.0314 bits (66), Expect = 6.662e-19
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 3 
Query:    3 IWFWMTAQPPKPS 41
            +WFWMT Q PKPS
Sbjct:  123 LWFWMTPQSPKPS 135
The following BLAST results are available for this feature:
BLAST of CV998064 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
CHI11_ORYSJ5.456e-2250.65Chitinase 11 OS=Oryza sativa subsp. japonica GN=Ch... [more]
CHI12_ORYSJ7.056e-2253.95Chitinase 12 OS=Oryza sativa subsp. japonica GN=Ch... [more]
CHI6_POPTR1.193e-2155.00Acidic endochitinase WIN6 OS=Populus trichocarpa G... [more]
CHIA_SECCE1.195e-2154.05Basic endochitinase A OS=Secale cereale GN=rsca PE... [more]
CHIA_SOLCI1.202e-2155.56Acidic endochitinase pcht28 OS=Solanum chilense PE... [more]
CHIB_VITVI1.555e-2150.00Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE... [more]
CHI8_ORYSJ1.562e-2156.94Chitinase 8 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIX_PEA3.422e-2157.75Endochitinase OS=Pisum sativum PE=2 SV=1[more]
CHIA_SOLLC2.170e-2054.17Acidic 26 kDa endochitinase OS=Solanum lycopersicu... [more]
CHIT_AVESA6.662e-1952.70Endochitinase (Fragments) OS=Avena sativa PE=1 SV=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS07_B-4_T3 Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07-153 Citrus sinensis cDNA clone TC28_D04_026, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV998064 ID=CV998064; Name=CV998064; organism=Citrus sinensis; type=EST; length=476bp
CAATCTGGTTCTGGATGACTGCACAGCCACCAAAGCCATCGTTGCCACGA
AGTCATTATCGACGAATGGAAACCATCAGCAAATGACGTAAACGCCAGCC
GGCTTCCGGGATACGGTCTAACCACAAATATAATCAACGGTGGAATTGAA
TGTGGCAAAGGTTGGAATAGCTTTGTAGCTAACCGTATTGGGTTCTTTAC
CACTTTCTGTGGCAAGTTTGGGATTCAGCCTGGCGACAATCTTGATTGCT
ACAACCAAGGGCCTTATGGCCCTAATTTGATGGCACAATCTATGTGATCA
TCATGATCAACGTTTTAGTAAAATAAGCTTAATAAAAGCTACTTAATAAA
AACTTTAGATGTCACACTTGTAGGTGGACAACTAAATTGTTAGTTTGTTA
CGCTCTTATTGTAGGTAAATTAAATAAGACTGATCCAACATGGATCAATG
AGTCTTCTCCAAAAAAAAAAAAAAAA
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