EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_XENLA (14-3-3 protein theta OS=Xenopus laevis GN=ywhaq PE=2 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIENSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_RAT (14-3-3 protein theta OS=Rattus norvegicus GN=Ywhaq PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIENSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_RABIT (14-3-3 protein theta OS=Oryctolagus cuniculus GN=YWHAQ PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIENSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_PONAB (14-3-3 protein theta OS=Pongo abelii GN=YWHAQ PE=2 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_MOUSE (14-3-3 protein theta OS=Mus musculus GN=Ywhaq PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIENSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_HUMAN (14-3-3 protein theta OS=Homo sapiens GN=YWHAQ PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_CHICK (14-3-3 protein theta OS=Gallus gallus GN=YWHAQ PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIENSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433T_BOVIN (14-3-3 protein theta OS=Bos taurus GN=YWHAQ PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.208e-11
Identity = 29/82 (35.37%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  +L L++ +L+ +A++ ES   YL M GD+     +      +  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   94 CTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.4242 bits (80), Expect = 1.208e-11
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   ++W+
Sbjct:    7 IQKAKLAEQAERYDDMATCMK--AVTEQGAELSNEERNLLSVAYKNVVGGRRSAWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433F_HUMAN (14-3-3 protein eta OS=Homo sapiens GN=YWHAH PE=1 SV=4)

HSP 1 Score: 53.1434 bits (126), Expect = 2.643e-11
Identity = 31/84 (36.90%), Postives = 44/84 (52.38%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSV--ESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C+ +LSL++  L+ + +    ES   YL M GD++    +  +   K      S   YK   EI+  Q+ PT PIRLGLALN S
Sbjct:   97 CNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFS 180

HSP 2 Score: 36.5798 bits (83), Expect = 2.643e-11
Identity = 21/53 (39.62%), Postives = 31/53 (58.49%), Query Frame = 3

Query:   39 AKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            A++A+Q   Y DMA  M   A +   + L+ +DR  LS AY NV+G   +SW+
Sbjct:   11 ARLAEQAERYDDMASAMK--AVTELNEPLSNEDRNLLSVAYKNVVGARRSSWR 61

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14331_CAEEL (14-3-3-like protein 1 OS=Caenorhabditis elegans GN=par-5 PE=1 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 4.452e-11
Identity = 37/124 (29.84%), Postives = 60/124 (48.39%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       +  +YRV +   + ++        C  +L L++  L+  A + ES   YL M GD++    +  +   +      S   Y+   +IA  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTEGSEKKQQLAKEYRVKVEQELNDI--------CQDVLKLLDEFLIVKAGAAESKVFYLKMKGDYYRYLAEVASED-RAAVVEKSQKAYQEALDIAKDKMQPTHPIRLGLALNFS 176

HSP 2 Score: 37.7354 bits (86), Expect = 4.452e-11
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQRAKLAEQAERYDDMAAAMKKVTEQ--GQELSNEERNLLSVAYKNVVGARRSSWR 62

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
1433T_XENLA	1.208e-11	35.37	14-3-3 protein theta OS=Xenopus laevis GN=ywhaq PE...	[more]
1433T_RAT	1.208e-11	35.37	14-3-3 protein theta OS=Rattus norvegicus GN=Ywhaq...	[more]
1433T_RABIT	1.208e-11	35.37	14-3-3 protein theta OS=Oryctolagus cuniculus GN=Y...	[more]
1433T_PONAB	1.208e-11	35.37	14-3-3 protein theta OS=Pongo abelii GN=YWHAQ PE=2...	[more]
1433T_MOUSE	1.208e-11	35.37	14-3-3 protein theta OS=Mus musculus GN=Ywhaq PE=1...	[more]
1433T_HUMAN	1.208e-11	35.37	14-3-3 protein theta OS=Homo sapiens GN=YWHAQ PE=1...	[more]
1433T_CHICK	1.208e-11	35.37	14-3-3 protein theta OS=Gallus gallus GN=YWHAQ PE=...	[more]
1433T_BOVIN	1.208e-11	35.37	14-3-3 protein theta OS=Bos taurus GN=YWHAQ PE=2 S...	[more]
1433F_HUMAN	2.643e-11	36.90	14-3-3 protein eta OS=Homo sapiens GN=YWHAH PE=1 S...	[more]
14331_CAEEL	4.452e-11	29.84	14-3-3-like protein 1 OS=Caenorhabditis elegans GN...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG