EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433E_RAT (14-3-3 protein epsilon OS=Rattus norvegicus GN=Ywhae PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.274e-21
Identity = 47/118 (39.83%), Postives = 70/118 (59.32%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EE+ G + ++ +     +M        C  IL +++ HL+P+A++ ES   Y  M GD+H    +F T   + + A NSL  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   61 RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 178

HSP 2 Score: 42.3578 bits (98), Expect = 3.274e-21
Identity = 25/56 (44.64%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY AK+A+Q   Y +M E M   A      +LT ++R  LS AY NVIG   ASW+
Sbjct:    8 VYQAKLAEQAERYDEMVESMKKVAGMDV--ELTVEERNLLSVAYKNVIGARRASWR 61

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433E_MOUSE (14-3-3 protein epsilon OS=Mus musculus GN=Ywhae PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.274e-21
Identity = 47/118 (39.83%), Postives = 70/118 (59.32%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EE+ G + ++ +     +M        C  IL +++ HL+P+A++ ES   Y  M GD+H    +F T   + + A NSL  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   61 RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 178

HSP 2 Score: 42.3578 bits (98), Expect = 3.274e-21
Identity = 25/56 (44.64%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY AK+A+Q   Y +M E M   A      +LT ++R  LS AY NVIG   ASW+
Sbjct:    8 VYQAKLAEQAERYDEMVESMKKVAGMDV--ELTVEERNLLSVAYKNVIGARRASWR 61

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433E_HUMAN (14-3-3 protein epsilon OS=Homo sapiens GN=YWHAE PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.274e-21
Identity = 47/118 (39.83%), Postives = 70/118 (59.32%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EE+ G + ++ +     +M        C  IL +++ HL+P+A++ ES   Y  M GD+H    +F T   + + A NSL  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   61 RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 178

HSP 2 Score: 42.3578 bits (98), Expect = 3.274e-21
Identity = 25/56 (44.64%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY AK+A+Q   Y +M E M   A      +LT ++R  LS AY NVIG   ASW+
Sbjct:    8 VYQAKLAEQAERYDEMVESMKKVAGMDV--ELTVEERNLLSVAYKNVIGARRASWR 61

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433E_CHICK (14-3-3 protein epsilon OS=Gallus gallus GN=YWHAE PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.274e-21
Identity = 47/118 (39.83%), Postives = 70/118 (59.32%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EE+ G + ++ +     +M        C  IL +++ HL+P+A++ ES   Y  M GD+H    +F T   + + A NSL  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   61 RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 178

HSP 2 Score: 42.3578 bits (98), Expect = 3.274e-21
Identity = 25/56 (44.64%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY AK+A+Q   Y +M E M   A      +LT ++R  LS AY NVIG   ASW+
Sbjct:    8 VYQAKLAEQAERYDEMVESMKKVAGMDV--ELTVEERNLLSVAYKNVIGARRASWR 61

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433E_BOVIN (14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.274e-21
Identity = 47/118 (39.83%), Postives = 70/118 (59.32%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EE+ G + ++ +     +M        C  IL +++ HL+P+A++ ES   Y  M GD+H    +F T   + + A NSL  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   61 RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS 178

HSP 2 Score: 42.3578 bits (98), Expect = 3.274e-21
Identity = 25/56 (44.64%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY AK+A+Q   Y +M E M   A      +LT ++R  LS AY NVIG   ASW+
Sbjct:    8 VYQAKLAEQAERYDEMVESMKKVAGMDV--ELTVEERNLLSVAYKNVIGARRASWR 61

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: RAD25_SCHPO (DNA damage checkpoint protein rad25 OS=Schizosaccharomyces pombe GN=rad25 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.198e-21
Identity = 45/118 (38.14%), Postives = 67/118 (56.78%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G   +  L     +   +     C  +LS++E HL+P+A++ ES   Y  M GD++    +F       + A +SL  YK+  +IA+ +L PT P+RLGLALN S
Sbjct:   62 RIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFS 179

HSP 2 Score: 45.4394 bits (106), Expect = 7.198e-21
Identity = 25/56 (44.64%), Postives = 34/56 (60.71%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY+AK+A+Q   Y +M E M   A S   D L+ ++R  LS AY N+IG   ASW+
Sbjct:    9 VYLAKLAEQAERYEEMVENMKKVACSN--DKLSVEERNLLSVAYKNIIGARRASWR 62

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433_DICDI (14-3-3-like protein OS=Dictyostelium discoideum GN=fttB PE=1 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 2.686e-20
Identity = 48/126 (38.10%), Postives = 71/126 (56.35%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFD--------YRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +        Y+  +   + ++        C+ IL ++ESHL+ S++S ES   Y  M GD+     +F T   +  +A +SL  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   60 RIISSIEQKEESKGNENHVKKIKEYKCKVEKELTDI--------CNDILEVLESHLIVSSASGESKVFYYKMKGDYFRYLAEFATGNPRKTSAESSLIAYKAASDIAVTELPPTHPIRLGLALNFS 177

HSP 2 Score: 45.8246 bits (107), Expect = 2.686e-20
Identity = 26/57 (45.61%), Postives = 35/57 (61.40%), Query Frame = 3

Query:   27 DVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +VYMAK+A+Q   Y +M E M   A+     +LT ++R  LS AY NVIG   ASW+
Sbjct:    6 NVYMAKLAEQAERYEEMVEAMKKVAELDV--ELTVEERNLLSVAYKNVIGARRASWR 60

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14312_ARATH (14-3-3-like protein GF14 iota OS=Arabidopsis thaliana GN=GRF12 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.196e-19
Identity = 44/118 (37.29%), Postives = 66/118 (55.93%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +I+SSI+Q EES G +  V    G  +   +     C  IL++I+ HL+P A+S E+   Y  M GD++    +F+T   + + A  SL  Y++  + A  +L  T PIRLGLALN S
Sbjct:   67 RIMSSIEQKEESKGNESNVKQIKGYRQKVEDELANICQDILTIIDQHLIPHATSGEATVFYYKMKGDYYRYLAEFKTEQERKEAAEQSLKGYEAATQAASTELPSTHPIRLGLALNFS 184

HSP 2 Score: 44.2838 bits (103), Expect = 2.196e-19
Identity = 24/56 (42.86%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VYMAK+++Q   Y +M E M   A+     +LT ++R  LS  Y NVIG   ASW+
Sbjct:   14 VYMAKLSEQAERYDEMVETMKKVARVN--SELTVEERNLLSVGYKNVIGARRASWR 67

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14333_ENTHI (14-3-3 protein 3 (Fragment) OS=Entamoeba histolytica PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 2.866e-19
Identity = 45/118 (38.14%), Postives = 67/118 (56.78%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +II+S++Q E++ G D  V +  G      +    +CD +L +I+ +LLP+AS+ ES   Y  M+GD++    +F     + + A  SL  Y    EI+   LAPT PIRLGLALN S
Sbjct:   58 RIITSLEQKEQAKGNDKHVEIIKGYRAKIEDELAKYCDDVLKVIKENLLPNASTSESKVFYKKMEGDYYRYYAEFTVDEKRQEVADKSLAAYTEATEISNADLAPTHPIRLGLALNFS 175

HSP 2 Score: 39.6614 bits (91), Expect = 2.866e-19
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V+++K+A+Q   Y +M ++M   A      +L+ ++R  LS AY NVIG   ASW+
Sbjct:    5 VFLSKLAEQSERYDEMVQYMKQVAALN--TELSVEERNLLSVAYKNVIGSRRASWR 58

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14310_ARATH (14-3-3-like protein GF14 epsilon OS=Arabidopsis thaliana GN=GRF10 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.837e-19
Identity = 46/118 (38.98%), Postives = 67/118 (56.78%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +I+SSI+Q EES G D  V       +   +     C+ ILS+I+ HL+PS+++VES   +  M GD++    +F +   + + A  SL  YK+    A   LAPT P+RLGLALN S
Sbjct:   62 RILSSIEQKEESKGNDENVKRLKNYRKRVEDELAKVCNDILSVIDKHLIPSSNAVESTVFFYKMKGDYYRYLAEFSSGAERKEAADQSLEAYKAAVAAAENGLAPTHPVRLGLALNFS 179

HSP 2 Score: 40.0466 bits (92), Expect = 4.837e-19
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY+AK+++Q   Y +M E M   A+     +LT ++R  +S  Y NVIG   ASW+
Sbjct:    9 VYLAKLSEQTERYDEMVEAMKKVAQLDV--ELTVEERNLVSVGYKNVIGARRASWR 62

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
1433E_RAT	3.274e-21	39.83	14-3-3 protein epsilon OS=Rattus norvegicus GN=Ywh...	[more]
1433E_MOUSE	3.274e-21	39.83	14-3-3 protein epsilon OS=Mus musculus GN=Ywhae PE...	[more]
1433E_HUMAN	3.274e-21	39.83	14-3-3 protein epsilon OS=Homo sapiens GN=YWHAE PE...	[more]
1433E_CHICK	3.274e-21	39.83	14-3-3 protein epsilon OS=Gallus gallus GN=YWHAE P...	[more]
1433E_BOVIN	3.274e-21	39.83	14-3-3 protein epsilon OS=Bos taurus GN=YWHAE PE=2...	[more]
RAD25_SCHPO	7.198e-21	38.14	DNA damage checkpoint protein rad25 OS=Schizosacch...	[more]
1433_DICDI	2.686e-20	38.10	14-3-3-like protein OS=Dictyostelium discoideum GN...	[more]
14312_ARATH	2.196e-19	37.29	14-3-3-like protein GF14 iota OS=Arabidopsis thali...	[more]
14333_ENTHI	2.866e-19	38.14	14-3-3 protein 3 (Fragment) OS=Entamoeba histolyti...	[more]
14310_ARATH	4.837e-19	38.98	14-3-3-like protein GF14 epsilon OS=Arabidopsis th...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG