EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_BOVIN (14-3-3 protein zeta/delta OS=Bos taurus GN=YWHAZ PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.345e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+AS  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 1.345e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 143B1_ONCMY (14-3-3 protein beta/alpha-1 OS=Oncorhynchus mykiss PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.749e-14
Identity = 39/124 (31.45%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L+L++++L+ +A+  ES   YL M GD++    +  +   K  T  NS   Y+   +I+   + PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTEGNEKKQQMAREYREKIEAELQDI--------CKDVLALLDNYLIANATQAESKVFYLKMKGDYYRYLSEVASGDSKKTTVENSQQAYQEAFDISKKDMQPTHPIRLGLALNFS 175

HSP 2 Score: 38.5058 bits (88), Expect = 1.749e-14
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAAAMK--AVTEQGGELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_RAT (14-3-3 protein zeta/delta OS=Rattus norvegicus GN=Ywhaz PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.271e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+AS  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNASQPESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 2.271e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_MOUSE (14-3-3 protein zeta/delta OS=Mus musculus GN=Ywhaz PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.271e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+AS  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNASQPESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 2.271e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 143B2_ONCMY (14-3-3 protein beta/alpha-2 OS=Oncorhynchus mykiss PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 2.272e-14
Identity = 39/124 (31.45%), Postives = 65/124 (52.42%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E      +M  +YR  +   + ++        C+ +L L++ +L+ +A++ ES   YL M GD++    +      K  T  NS   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTEGNEKKQAMAKEYREKIETELQDI--------CNDVLGLLDKYLIANATAAESKVFYLKMKGDYYRYLSEVAAGDAKKTTVDNSQQAYQDAFDISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 2.272e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAGAMKSVTEQ--GGELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 143BB_XENLA (14-3-3 protein beta/alpha-B OS=Xenopus laevis GN=ywhab-B PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.952e-14
Identity = 41/124 (33.06%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L L++  L+P+A+  ES   YL M GD++    +  +   K +T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTEGNDKRQQMAREYREKVETELQDI--------CKDVLDLLDRFLVPNATPPESKVFYLKMKGDYYRYLSEVASGDSKQETVANSQQAYQEAFEISKSEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.8094 bits (81), Expect = 2.952e-14
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+++Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLSEQAERYDDMAASMK--AVTELGAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433_EIMTE (14-3-3 protein OS=Eimeria tenella PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 3.818e-14
Identity = 39/118 (33.05%), Postives = 54/118 (45.76%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISS++Q E +        L     +   N     C  IL+L+   LLP  +  ES   Y  M GD++    +F     K  +A  +   YK   + A  +L  T PIRLGLALN S
Sbjct:   78 RIISSVEQKEANRNHMANKALAASYRQKVENELNKICQEILTLLTDKLLPRTTDSESRVFYFKMKGDYYRYISEFSNEEGKKASAEQAEESYKRATDTAEAELPSTHPIRLGLALNYS 195

HSP 2 Score: 43.5134 bits (101), Expect = 3.818e-14
Identity = 24/66 (36.36%), Postives = 35/66 (53.03%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKS----------THADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY AK+A+Q   Y +MAE M +  ++             D+LT ++R  LS AY N +G   ASW+
Sbjct:   13 VYRAKLAEQAERYDEMAEAMKNLVENCLDQNNSPPGAKGDELTVEERNLLSVAYKNAVGARRASWR 78

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_XENTR (14-3-3 protein zeta OS=Xenopus tropicalis GN=ywhaz PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.834e-14
Identity = 39/124 (31.45%), Postives = 63/124 (50.81%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + E+        C+ +L+L++  L+ +AS  ES   YL M GD++    +  +   K D    S   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQEMSREYREKIEAELREI--------CNDVLNLLDKFLIANASQPESKVFYLKMKGDYYRYLAEVASGDAKADIVAQSQKAYQDAFDISKTEMQPTHPIRLGLALNFS 175

HSP 2 Score: 37.7354 bits (86), Expect = 3.834e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKRVTEE--GGELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14331_SCHMA (14-3-3 protein homolog 1 OS=Schistosoma mansoni PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 4.977e-14
Identity = 40/125 (32.00%), Postives = 65/125 (52.00%), Query Frame = 1

Query:  178 WEWLHGKIISSI-DQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            W  +HG  + ++ D+ ++ +  +YR+      +E + N     C+ +L+L+E +LLP+AS  +S   +L M GD++    +  T   +T+    SL  Y      A   L  T PIRLGLALN S
Sbjct:   70 WRVIHGSEMKAVNDRTKKQIAEEYRI-----KMEKELN---TICNQVLALLEDYLLPNASPDDSKVFFLKMQGDYYRYLAEVATDDARTEVVQKSLDAYTKA-TTAAENLPTTHPIRLGLALNFS 185

HSP 2 Score: 39.2762 bits (90), Expect = 4.977e-14
Identity = 20/56 (35.71%), Postives = 35/56 (62.50%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V++AK+A+Q   Y DMA  M  + +++   +L  ++R  LS AY NV+G   ++W+
Sbjct:   18 VHIAKLAEQAERYDDMAAAMKRYTEAS--GNLGNEERNLLSVAYKNVVGARRSAWR 71

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_AEDAE (14-3-3 protein zeta OS=Aedes aegypti GN=14-3-3zeta PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 4.979e-14
Identity = 41/124 (33.06%), Postives = 63/124 (50.81%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  ES      +  +YR  +   + E+        C  +L L++  L+P AS+ ES   YL M GD++    +  T   +     +S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   63 RVISSIEQKTESSARKQQLAREYRERVEKELREI--------CYEVLGLLDKFLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRNTVVDDSQAAYQDAFEISKGKMQPTHPIRLGLALNFS 178

HSP 2 Score: 37.3502 bits (85), Expect = 4.979e-14
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA+ M    ++    +L+ ++R  LS AY NV+G   +SW+
Sbjct:   10 VQKAKLAEQSERYDDMAQAMKSVTET--GVELSNEERNLLSVAYKNVVGARRSSWR 63

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
1433Z_BOVIN	1.345e-14	33.06	14-3-3 protein zeta/delta OS=Bos taurus GN=YWHAZ P...	[more]
143B1_ONCMY	1.749e-14	31.45	14-3-3 protein beta/alpha-1 OS=Oncorhynchus mykiss...	[more]
1433Z_RAT	2.271e-14	33.06	14-3-3 protein zeta/delta OS=Rattus norvegicus GN=...	[more]
1433Z_MOUSE	2.271e-14	33.06	14-3-3 protein zeta/delta OS=Mus musculus GN=Ywhaz...	[more]
143B2_ONCMY	2.272e-14	31.45	14-3-3 protein beta/alpha-2 OS=Oncorhynchus mykiss...	[more]
143BB_XENLA	2.952e-14	33.06	14-3-3 protein beta/alpha-B OS=Xenopus laevis GN=y...	[more]
1433_EIMTE	3.818e-14	33.05	14-3-3 protein OS=Eimeria tenella PE=2 SV=1	[more]
1433Z_XENTR	3.834e-14	31.45	14-3-3 protein zeta OS=Xenopus tropicalis GN=ywhaz...	[more]
14331_SCHMA	4.977e-14	32.00	14-3-3 protein homolog 1 OS=Schistosoma mansoni PE...	[more]
1433Z_AEDAE	4.979e-14	33.06	14-3-3 protein zeta OS=Aedes aegypti GN=14-3-3zeta...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG