CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_VIBCH (Dihydrolipoyl dehydrogenase OS=Vibrio cholerae GN=lpd PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.256e-18
Identity = 71/239 (29.71%), Postives = 121/239 (50.63%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHE-KIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVA-----DVVLFATGRAP 705
            GG C+  GC+P K L++ A    E +    +G    E K D D  +L ++K   I +L G    +     V +  G GK  GPN +EV   +G K   +  + ++A GSR  + P IP ++  I  S +AL L+E+P + +++GGG I +E A+++  +GS +D++   +  +   D +M  V  + ++ +  NL   T +  +   E+G+  ++  G+   A     D VL A GR P
Sbjct:   42 GGVCLNVGCIPSKALLHVAKVIEEAKALTEHGIVFGEPKTDIDKVRLWKEKV--INQLTGGLAGMAKMRKVNVVNGYGKFTGPNTIEVDGEEG-KTVVTFDNAIVAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLLIMGGGIIGLEMATVYHSLGSKIDVVEMFDQLIPAADKDMVKVYTKRIKDK-FNLMLETKVTAVEAKEDGI-YVSMEGKSAPAQAERYDAVLVAIGRVP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_SCHPO (Dihydrolipoyl dehydrogenase, mitochondrial OS=Schizosaccharomyces pombe GN=dld1 PE=2 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 5.553e-18
Identity = 69/244 (28.28%), Postives = 119/244 (48.77%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELE-DARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTK-LSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRG--FDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVK-----RITDHGEEIVADVVLFATGRAP 705
            GGTC+  GC+P K L+  +     ++ D +  G +V   +  +  ++++ K D +  L    + L     V+  +G G  + P  + V  +DG    +  AK+ +IATGS  +  P PG    ++  ++S  ALSL E+PK+  VLGGG I +E  S+W  +G+ V ++  + LP  G   D ++   ++R +  +GI     T +     + + V+        +  E    DV+L A GR P
Sbjct:   81 GGTCLNVGCIPSKALLNNSHIYHTVKHDTKRRGIDV-SGVSVNLSQMMKAKDDSVKSLTSGIEYLFKKNKVEYAKGTGSFIDPQTLSVKGIDGAADQTIKAKNFIIATGSEVK--PFPGVTIDEKKIVSSTGALSLSEVPKKMTVLGGGIIGLEMGSVWSRLGAEVTVV--EFLPAVGGPMDADISKALSRIISKQGIKFKTSTKLLSAKVNGDSVEVEIENMKNNKRETYQTDVLLVAIGRVP 319          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_HALSA (Dihydrolipoyl dehydrogenase OS=Halobacterium salinarium GN=lpdA PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.253e-18
Identity = 67/241 (27.80%), Postives = 116/241 (48.13%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAK--HILIATGSRAQRAP--IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEG--VKRITDHGE--EIVADVVLFATGRAP 705
            A GGTC+  GC+P K ++  +    E   A   G  V+   D D  +++  K   + +L G  ++L    GV L EG  +  G +++ V      + S + +  H +++TGSR    P    G +  + S +AL++ ELP   V++GGGYI +E ++++  +G  V ++   +  L  + D++   V +  E  GI+ H          +++G  V    + GE  E   + VL A GR P
Sbjct:   42 AYGGTCLNYGCIPSKAMITASGVAHEAGHAEEMG--VYADPDVDVAEMVDWKDGVVDQLTGGVEKLCKANGVNLIEGRAEFAGSDKLRVVHGGDGQGSETIEYEHAIVSTGSRPIEVPGFDFGDDPVLDSRQALAMAELPSSMVIVGGGYIGMELSTVFAKLGVDVTVVEMLDGILPQYGDDIARPVRQRAEELGIDFHFGLAADSWTDTDDGIVVTAADEDGEETEFETEKVLVAVGRQP 280          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_CHLP8 (Dihydrolipoyl dehydrogenase OS=Chlorobaculum parvum (strain NCIB 8327) GN=lpd PE=3 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 9.472e-18
Identity = 73/236 (30.93%), Postives = 117/236 (49.58%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQE----LAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKR-ITDHGEE---IVADVVLFATG 696
            GG CV  GC+P K L+  A      ++   +G  V E + FD  + +++     L+ +   + +L  A V+++ GE  + G   V+VT  DG++ S  A +I++ATG  AQ   IPG E      ITS EAL L+++P+  +V+GGG I VE A  +   G+ V ++      L   + E+   + R+ E   I +     +  +  SE GV   +   G+E   I A  +L A G
Sbjct:   45 GGVCVNWGCIPTKALLRSAEVYDLAKNPSEFGVNVSE-LSFDLAQAVKRSRKVSLKSSKGVEFMLKKAKVEVWRGEAVLTGSKGVKVTAEDGSERSLEAANIIVATG--AQPRVIPGLEPDGKKIITSREALILKDVPESMIVVGGGAIGVEMAWFYAKAGAKVTIVELMPRLLPAEEAEVSEALKRSFEKVDITVQCGAKLGNVAISEFGVNADLLAEGKEPQKIEASCMLVAVG 277          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_SHIFL (Dihydrolipoyl dehydrogenase OS=Shigella flexneri GN=lpdA PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.616e-17
Identity = 73/239 (30.54%), Postives = 119/239 (49.79%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHE-KIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVA-----DVVLFATGRAP 705
            GG C+  GC+P K L++ A    E +    +G    E K D D  K+   K   I +L G    +     VK+  G GK  G N +EV   +G K   +  + +IA GSR  + P IP ++  I  S +AL L+E+P+R +V+GGG I +E  +++  +GS +D++   +  +   D ++  V  + +  +  NL   T +  +   E+G+  +T  G++  A     D VL A GR P
Sbjct:   42 GGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDID--KIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENG-KTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRI-SKKFNLMLETKVTAVEAKEDGI-YVTMEGKKAPAEPQRYDAVLVAIGRVP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_ECOLI (Dihydrolipoyl dehydrogenase OS=Escherichia coli (strain K12) GN=lpdA PE=1 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.616e-17
Identity = 73/239 (30.54%), Postives = 119/239 (49.79%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHE-KIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVA-----DVVLFATGRAP 705
            GG C+  GC+P K L++ A    E +    +G    E K D D  K+   K   I +L G    +     VK+  G GK  G N +EV   +G K   +  + +IA GSR  + P IP ++  I  S +AL L+E+P+R +V+GGG I +E  +++  +GS +D++   +  +   D ++  V  + +  +  NL   T +  +   E+G+  +T  G++  A     D VL A GR P
Sbjct:   42 GGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDID--KIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENG-KTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRI-SKKFNLMLETKVTAVEAKEDGI-YVTMEGKKAPAEPQRYDAVLVAIGRVP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_ECOL6 (Dihydrolipoyl dehydrogenase OS=Escherichia coli O6 GN=lpdA PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.616e-17
Identity = 73/239 (30.54%), Postives = 119/239 (49.79%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHE-KIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVA-----DVVLFATGRAP 705
            GG C+  GC+P K L++ A    E +    +G    E K D D  K+   K   I +L G    +     VK+  G GK  G N +EV   +G K   +  + +IA GSR  + P IP ++  I  S +AL L+E+P+R +V+GGG I +E  +++  +GS +D++   +  +   D ++  V  + +  +  NL   T +  +   E+G+  +T  G++  A     D VL A GR P
Sbjct:   42 GGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDID--KIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENG-KTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRI-SKKFNLMLETKVTAVEAKEDGI-YVTMEGKKAPAEPQRYDAVLVAIGRVP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_ECO57 (Dihydrolipoyl dehydrogenase OS=Escherichia coli O157:H7 GN=lpdA PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.616e-17
Identity = 73/239 (30.54%), Postives = 119/239 (49.79%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHE-KIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVA-----DVVLFATGRAP 705
            GG C+  GC+P K L++ A    E +    +G    E K D D  K+   K   I +L G    +     VK+  G GK  G N +EV   +G K   +  + +IA GSR  + P IP ++  I  S +AL L+E+P+R +V+GGG I +E  +++  +GS +D++   +  +   D ++  V  + +  +  NL   T +  +   E+G+  +T  G++  A     D VL A GR P
Sbjct:   42 GGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDID--KIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENG-KTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRI-SKKFNLMLETKVTAVEAKEDGI-YVTMEGKKAPAEPQRYDAVLVAIGRVP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH2_BACSU (Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=bfmBC PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.110e-17
Identity = 61/202 (30.20%), Postives = 106/202 (52.48%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPN-------EVEVTQLDGTKLSYSA-KHILIATGSRAQRAPIPGQEL----AITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGI 582
            GGTC+ +GC+P K L+  A       +A  +G E    +  +++K+ Q+K   + +L      L+    + +Y G G+I+GP+        + V + +G +      K ++IATGSR +   +PG E+     +TSDEAL +EELP+  +++GGG I +E+AS+    G  V ++   +  L   D E+   +   L+ +GI
Sbjct:   39 GGTCLHKGCIPSKALLRSAEVYRTAREADQFGVET-AGVSLNFEKVQQRKQAVVDKLAAGVNHLMKKGKIDVYTGYGRILGPSIFSPLPGTISVERGNGEENDMLIPKQVIIATGSRPRM--LPGLEVDGKSVLTSDEALQMEELPQSIIIVGGGVIGIEWASMLHDFGVKVTVIEYADRILPTEDLEISKEMESLLKKKGI 237          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_TRYCR (Dihydrolipoyl dehydrogenase OS=Trypanosoma cruzi GN=LPD PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.599e-17
Identity = 54/168 (32.14%), Postives = 86/168 (51.19%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELE-DARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IP-GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLL 501
            A GGTC+  GC+P K L++      +   +   YG    E +  D  K+ Q+K   +  L G  + L     V  Y+GEG     + + V  LDG +  +  K  +IATGS     P +P  +++ ++S  AL+L  +PK  VV+GGG I +E  S+W  +G+ V ++
Sbjct:   45 ALGGTCLNVGCIPSKALLHATHVYHDAHANFARYGLMGGEGVTMDSAKMQQQKERAVKGLTGGVEYLFKKNKVTYYKGEGSFETAHSIRVNGLDGKQEMFETKKTIIATGSEPTELPFLPFDEKVVLSSTGALALPRVPKTMVVIGGGVIGLELGSVWARLGAKVTVV 212          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
DLDH_VIBCH3.256e-1829.71Dihydrolipoyl dehydrogenase OS=Vibrio cholerae GN=... [more]
DLDH_SCHPO5.553e-1828.28Dihydrolipoyl dehydrogenase, mitochondrial OS=Schi... [more]
DLDH_HALSA7.253e-1827.80Dihydrolipoyl dehydrogenase OS=Halobacterium salin... [more]
DLDH_CHLP89.472e-1830.93Dihydrolipoyl dehydrogenase OS=Chlorobaculum parvu... [more]
DLDH_SHIFL1.616e-1730.54Dihydrolipoyl dehydrogenase OS=Shigella flexneri G... [more]
DLDH_ECOLI1.616e-1730.54Dihydrolipoyl dehydrogenase OS=Escherichia coli (s... [more]
DLDH_ECOL61.616e-1730.54Dihydrolipoyl dehydrogenase OS=Escherichia coli O6... [more]
DLDH_ECO571.616e-1730.54Dihydrolipoyl dehydrogenase OS=Escherichia coli O1... [more]
DLDH2_BACSU2.110e-1730.20Dihydrolipoyl dehydrogenase OS=Bacillus subtilis G... [more]
DLDH_TRYCR3.599e-1732.14Dihydrolipoyl dehydrogenase OS=Trypanosoma cruzi G... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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