CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_SYNY3 (Dihydrolipoyl dehydrogenase OS=Synechocystis sp. (strain PCC 6803) GN=lpdA PE=1 SV=3)

HSP 1 Score: 88.5817 bits (218), Expect = 3.599e-17
Identity = 74/249 (29.72%), Postives = 117/249 (46.99%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDA---RSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPI--PGQEL----AITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNL-EGRGINLHP---RTTIK-----ELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCV RGC+P K L+  +    E+ D    +  G +++  + F  + +     D + ++       L+   V    G GK+ GP EV V   + T++   AK I++  GS     P   PG E+      TSDEA+ LE LP+   ++G GYI +EF+ ++  +G  V ++      + GFD E+  +  R L + R I  +     T IK     E+  ++   K + D    +  D  L ATGR P
Sbjct:   41 GGTCVNRGCIPSKALLAASGRVREMSDQDHLQQLGIQING-VTFTREAIAAHANDLVSKIQSDLTNSLTRLKVDTIRGWGKVSGPQEVTVIGDNETRI-LKAKEIMLCPGS----VPFVPPGIEIDHKTVFTSDEAVKLETLPQWIAIIGSGYIGLEFSDVYTALGCEVTMIEALPDLMPGFDPEIAKIAERVLIKSRDIETYTGVFATKIKAGSPVEIELTDAKTKEVID---TLEVDACLVATGRIP 280          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: YKGC_ECOLI (Probable pyridine nucleotide-disulfide oxidoreductase ykgC OS=Escherichia coli (strain K12) GN=ykgC PE=3 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 6.140e-17
Identity = 66/235 (28.09%), Postives = 114/235 (48.51%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRL-NGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELA---ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTC+  GC+P K LV+         DA+ +          D+ + +Q+K + +  L N  +  L     + + +G+ + +  + + V + +G  L    + I I TG++    PIPG         S   L+L+ELP    +LGGGYI VEFAS++   GS V +L    L L   D ++   +A  L  +G+++     ++ +   E  V+  ++H  ++  D +L A+GR P
Sbjct:   40 GGTCINIGCIPTKTLVH---------DAQQHT---------DFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFINNHSLRVHRPEGN-LEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGHLGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIILNAHVERISHHENQVQVHSEHA-QLAVDALLIASGRQP 254          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_RALEH (Dihydrolipoyl dehydrogenase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=odhL PE=3 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 8.019e-17
Identity = 68/238 (28.57%), Postives = 113/238 (47.48%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGAS-FGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDE--ALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVK-RITD---HGEEIVADVVLFATGRAP 705
            GGTC+  GC+P K L+  +  F         +G  V + +  D  K+L++K D + ++    + L     V L +G GK VG +  E  Q+D      +AK ++IATGS+A+  P    +  + SD   AL    +PK+  V+G G I +E  S+WR +GS V +L      L   D+ +     + L  +G+       + E+   + GV  + TD     + +  D ++ + GR P
Sbjct:   48 GGTCLNVGCIPSKALLASSEEFENVQHHLGDHGITVGD-VKVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGYGKFVGKS-AEGFQVDVAGEVVTAKQVIIATGSKARHLPGIKVDNDLVSDNEGALKFPAVPKKLGVIGAGVIGLELGSVWRRLGSDVTVLEALPAFLGAADEGVAKEAQKQLTKQGLKFSLGVNVNEVTTGKNGVTVKYTDKDGKAQTLEVDRLIVSVGRVP 283          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_CHLMU (Dihydrolipoyl dehydrogenase OS=Chlamydia muridarum GN=lpdA PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.368e-16
Identity = 59/233 (25.32%), Postives = 114/233 (48.93%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELA---ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GGTC+ RGC+P K L+  A    ++  A  +G  ++     D+  ++Q+K   +  +      L+ +  + ++ G G ++   EV++  L  T     A+ I++ATGS  +  P +P  + +   + S   L+L+E+P++  ++GGG I  EFAS++  +GS V ++   +  L   + ++   +       GI      ++  +    + V R+T +G     D VL + GR
Sbjct:   39 GGTCLNRGCIPSKALLASAEIVAQIRHADQFGIHING-FSIDYPAMVQRKDTVVRSIRDGLNGLIRSNKITVFSGRGSLISSTEVKI--LGETPSVIKAQSIILATGSEPRAFPGVPFSQQSPRILCSTGVLNLKEIPQKMAIIGGGVIGCEFASLFHTLGSEVSVIEASQQILALNNPDISKTMFDKFTRHGIRFMLGASVSSIEDMGDRV-RLTINGNIEEYDYVLVSIGR 267          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_ACICA (Mercuric reductase OS=Acinetobacter calcoaceticus GN=merA PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.786e-16
Identity = 72/235 (30.64%), Postives = 107/235 (45.53%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRL-NGIYKRLLS-NAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAI--TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCV  GCVP KI++  A       ++   G         D  KLL ++   +  L +  Y+ +L  N  + +  GE +      + V   DG +   +    L+ATG+     PIPG + +   TS EAL  + LP+R  V+G   +A+E A  +  +GS V +L R  L  R  D  +   V       GI +   T   ++   +      T +G EI AD +L ATGRAP
Sbjct:  133 GGTCVNVGCVPSKIMIRAAHIAHLRRESPFDGGIAATVPAIDRSKLLAQQQARVEELRHAKYEGILDGNPAITVVHGEARFKDEQSLVVRLNDGGERVVAFDRCLVATGASPAVPPIPGLKESPYWTSTEALVSDTLPERLAVIGSSVVALELAQAFARLGSQVTILARNTLFFRD-DPAIGEAVTAAFRAEGIKVLEHTQASQVAHVDGEFVLTTGYG-EIRADQLLVATGRAP 365          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_COLP3 (Soluble pyridine nucleotide transhydrogenase OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=sthA PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.333e-16
Identity = 65/231 (28.14%), Postives = 106/231 (45.89%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQE--LAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L    S   E      +      K    ++ +L   +  I +   +     +   V+  +G+   +  + + ++  DG+    SAK I+IATGSR  R      +      SD  LSL+  P+  ++ G G I  E+ASI+RG+G  VDL+  +E  L   D EM   ++ +L   G+ +     I+ +  SE+ V      G+++ AD +LFA GR
Sbjct:   42 GGGCTHWGTIPSKALRQSVSRLIEYNSNPLFNQNEQVK-QLTFQDILSHASAVIQKQVSLRSGFYNRNRVEHIQGQASFIDAHTISISHPDGSVEKISAKQIMIATGSRPYRPDDIDFDHPRVYDSDSILSLKHAPQHVIIYGAGVIGSEYASIFRGLGVKVDLINTRERLLSFLDTEMSDSLSYHLWNSGVVIRHGEEIERVESSEDAVIVHLKSGKKMRADCLLFANGR 271          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_VIBVY (Soluble pyridine nucleotide transhydrogenase OS=Vibrio vulnificus (strain YJ016) GN=sthA PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.047e-16
Identity = 64/235 (27.23%), Postives = 111/235 (47.23%), Query Frame = 1
Query:    1 DSAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRA-QRAPIP-GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            +S+ GG C   G +P K L +  S   E      +  + +  +   +  +L      I +   + +        +L  G  +    + + VTQ DGT+  Y+A   +IATGSR  Q A +    E    SD  LSL+  P+  ++ G G I  E+ASI+RG+G   DL+  ++  L   D+E+   ++ +    G+ +    T + +  +E+GV      G+++ AD +L+A GR
Sbjct:   38 ESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLF-CKNNSSLHATFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFTDAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNHERIYDSDSILSLKHDPRHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETYERIEGTEDGVIVHLQSGKKMKADCLLYANGR 271          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_VIBVU (Soluble pyridine nucleotide transhydrogenase OS=Vibrio vulnificus GN=sthA PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.047e-16
Identity = 64/235 (27.23%), Postives = 111/235 (47.23%), Query Frame = 1
Query:    1 DSAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRA-QRAPIP-GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            +S+ GG C   G +P K L +  S   E      +  + +  +   +  +L      I +   + +        +L  G  +    + + VTQ DGT+  Y+A   +IATGSR  Q A +    E    SD  LSL+  P+  ++ G G I  E+ASI+RG+G   DL+  ++  L   D+E+   ++ +    G+ +    T + +  +E+GV      G+++ AD +L+A GR
Sbjct:   38 ESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLF-CKNNSSLHATFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFTDAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNHERIYDSDSILSLKHDPRHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETYERIEGTEDGVIVHLQSGKKMKADCLLYANGR 271          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_VIBF1 (Soluble pyridine nucleotide transhydrogenase OS=Vibrio fischeri (strain ATCC 700601 / ES114) GN=sthA PE=3 SV=2)

HSP 1 Score: 85.5001 bits (210), Expect = 3.047e-16
Identity = 66/231 (28.57%), Postives = 104/231 (45.02%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +  S   E      Y  + +  +   +  +L      I +   + +         L  GE   V  N V VT  DG+  +Y+A   +IATGSR  R             SD  LSL+  P+  ++ G G I  E+ASI+RG+G  VDL+  ++  L   D+EM   ++ +    GI        + +  +++GV    + G+++ AD +LFA GR
Sbjct:   47 GGGCTHWGTIPSKALRHAVSRIIEFNSNPLY-CKNNTSLHSTFSDILGHAKSVIDKQTRMRQGFYDRNQCSLIFGEASFVEKNTVAVTAKDGSIETYTADKFIIATGSRPYRPEGINFNHSRVYDSDSILSLKHDPRHIIIYGAGVIGSEYASIFRGLGVKVDLVNTRDRLLSFLDNEMSDALSYHFWNSGIVTRNDENFEHIEANDDGVIMHLESGKKMKADCILFANGR 276          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_VIBPA (Soluble pyridine nucleotide transhydrogenase OS=Vibrio parahaemolyticus GN=sthA PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.980e-16
Identity = 63/235 (26.81%), Postives = 109/235 (46.38%), Query Frame = 1
Query:    1 DSAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            +S+ GG C   G +P K L +  S   E      +    +  +   +  +L      I +   + +         L  G  + +  + + V Q DGT+ +YSA   +IATGSR  R        E    SD  LSL+  P+  ++ G G I  E+ASI+RG+G   DL+  ++  L   D+E+   ++ +    G+ +    T +++  +E+GV      G+++ AD +L+A GR
Sbjct:   38 ESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLF-CRNNTSLHATFSDILGHAKTVIDKQTRLRQGFYDRNDCTLLFGTARFIDTHSIAVMQNDGTEETYSADKFVIATGSRPYRPSDVDFLHERIYDSDSILSLKHDPRHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLEFLDNEVSDALSYHFWNSGVVIRNDETYEKIEGTEDGVIIHLQSGKKMRADCLLYANGR 271          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
DLDH_SYNY33.599e-1729.72Dihydrolipoyl dehydrogenase OS=Synechocystis sp. (... [more]
YKGC_ECOLI6.140e-1728.09Probable pyridine nucleotide-disulfide oxidoreduct... [more]
DLDH_RALEH8.019e-1728.57Dihydrolipoyl dehydrogenase OS=Ralstonia eutropha ... [more]
DLDH_CHLMU1.368e-1625.32Dihydrolipoyl dehydrogenase OS=Chlamydia muridarum... [more]
MERA_ACICA1.786e-1630.64Mercuric reductase OS=Acinetobacter calcoaceticus ... [more]
STHA_COLP32.333e-1628.14Soluble pyridine nucleotide transhydrogenase OS=Co... [more]
STHA_VIBVY3.047e-1627.23Soluble pyridine nucleotide transhydrogenase OS=Vi... [more]
STHA_VIBVU3.047e-1627.23Soluble pyridine nucleotide transhydrogenase OS=Vi... [more]
STHA_VIBF13.047e-1628.57Soluble pyridine nucleotide transhydrogenase OS=Vi... [more]
STHA_VIBPA3.980e-1626.81Soluble pyridine nucleotide transhydrogenase OS=Vi... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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