CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_YERE8 (Soluble pyridine nucleotide transhydrogenase OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=sthA PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.369e-15
Identity = 60/231 (25.97%), Postives = 101/231 (43.72%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +  S   E      Y  +    I   +  +L      I +   + +        +++ G+   +  N + V   DGT  +  A +I+IATGSR  R        E    SD  L L   P+  ++ G G I  E+ASI+RG+   VDL+  ++  L   D EM   ++ +    G+ +      +++  + +GV      G+++ AD +L+A GR
Sbjct:   42 GGGCTHWGTIPSKALRHAVSRIIEFNQNPLYS-DNARTISSSFSDILNHADRVINQQTRMRQGFYDRNHCQMFSGDASFIDANTINVRYADGTNDTLRADNIVIATGSRPYRPANVDFTHERIYDSDTILQLSHEPQHVIIYGAGVIGCEYASIFRGLSVKVDLINTRDRLLAFLDQEMSDALSYHFWNNGVVIRHNEEFEQIEGTVDGVIVHLKSGKKVKADCLLYANGR 271          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_PHOLL (Soluble pyridine nucleotide transhydrogenase OS=Photorhabdus luminescens subsp. laumondii GN=sthA PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.369e-15
Identity = 68/243 (27.98%), Postives = 102/243 (41.98%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRL------------NGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +  S   E      Y          D  ++L+    EILR              G Y+R     G +++ GE   +  + V V   D      SA  I+IATGSR    P           SD  L L+  P+  ++ G G I  E+ASI+RG+G  VDL+  +   L   D EM   ++ +    GI +       ++   ++GV      G+++ AD +L+A GR
Sbjct:   41 GGGCTHWGTIPSKALRHAVSRIIEFNQNPLYS---------DNSRVLRSSFAEILRRAEMVINQQTRMRQGFYER----NGCRMFSGEATFIDDHRVSVRYADDNHDILSADKIIIATGSRPYCPPDVDFTHSRIYNSDSILKLDHEPRHVIIYGAGVIGCEYASIFRGLGVKVDLINTRNHLLAFLDQEMSDALSYHFWNSGIVIRHNEEYSKIEGVDDGVIVHLKSGKKVKADCLLYANGR 270          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_THIFE (Mercuric reductase OS=Thiobacillus ferrooxidans GN=merA PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 3.369e-15
Identity = 73/240 (30.42%), Postives = 105/240 (43.75%), Query Frame = 1
Query:    4 SAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLL---QKKTDEILRLNGIYKRLLS-NAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAI--TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            S  GGTCV  GCVP KI++  A       ++   G             LL   Q + DE+      Y+ +L  N  + +  GE +      V V   DG +        L+ATG+     PIPG +     TS E L  E +P+R  V+G   +A+E A  +  +GS V +L R  L LR  D  +   +       GI +   T   ++  ++      T HG E+ AD +L ATGRAP
Sbjct:  116 STIGGTCVNIGCVPSKIMIRAAHIAHLRRESPFDGGIQAVAPTIQRTALLVQQQARVDELRHAK--YEGILDGNPAITVLRGEARFKDSRSVVVHLNDGGERVVMFDRCLVATGASPAVPPIPGLKDTPYWTSTEGLVSESIPERLAVIGSSVVALELAQAFARLGSHVTILARGTLFLRE-DPAIGEAITAAFRAEGIEVLEHTQASQVAYADGEFVLATGHG-ELRADKLLVATGRAP 351          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_HAMD5 (Soluble pyridine nucleotide transhydrogenase OS=Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) GN=sthA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.400e-15
Identity = 67/237 (28.27%), Postives = 106/237 (44.73%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAIT------SDEALSL--EELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +  S   E      Y  E  + I   +  +LQ     I +   +           L+ G  + + P ++EV + DGT  + SA HI+IATGSR    P   + +  T      SD  L L  E+ PK  ++ G G I  E+ASI+RG+G  VDL+  +   L   D E+   ++ +    G+ +        +   ++GV      G+++ AD + +A GR
Sbjct:   42 GGGCTHSGTIPSKALRHVISRIIEFNQNPLYS-EHLQSIHPSFYHILQHANHVIRKQVSMRFDFYQRNHCHLFFGHARFIDPYKLEVIRPDGTLNTLSADHIVIATGSR----PYHPESIDFTHPRIYDSDSILELKEEQQPKHIIIYGAGVIGCEYASIFRGLGVKVDLINTRNHLLAFLDQEISDALSYHFWNNGVVIRHNEEFDLIEGLDQGVVVHLKSGKKVKADCLFYANGR 273          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_MARMS (Soluble pyridine nucleotide transhydrogenase OS=Marinomonas sp. (strain MWYL1) GN=sthA PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 9.802e-15
Identity = 66/235 (28.09%), Postives = 105/235 (44.68%), Query Frame = 1
Query:    4 SAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRA 702
            S  GG+C   G +P K L +             +  ++ E   F + K+L +    I +         +   + +Y G GK    N +EV   +       AK ++IATGSR  R A I      I  SD  LSL   P+  ++ G G I  E+ASI+ G+G  V+L+   +  L   DDE+   ++ +L   G+ +    T   +  +E GV      G+++ AD +LF  GR+
Sbjct:   38 SQVGGSCTHLGTIPSKALRHAVKEIIAFNTNPMFR-DIGEPRWFSFPKVLDRANRVIDKQVMGRTEYYARNRIDIYFGRGKFKDANTIEVNTYEKGPELLEAKKVVIATGSRPYRPANIDFSHPRIYCSDTILSLSHTPRSLIIYGAGVIGCEYASIFCGLGVRVELINPAKKLLSFLDDEITDALSYHLRDGGVLIRHNETYDSVETTERGVVMHMASGKKLRADALLFCNGRS 271          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_VIBPA (Dihydrolipoyl dehydrogenase OS=Vibrio parahaemolyticus GN=lpd PE=3 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 9.802e-15
Identity = 63/241 (26.14%), Postives = 118/241 (48.96%), Query Frame = 1
Query:    4 SAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVA-----DVVLFATGRAP 705
            S  GG C+  GC+P K L++ +    E +    +G  V  +   D  K+   K   + +L G    +     V +  G GK  GPN + V + +G     +  + ++A GSR  + P IP ++  I  S +AL L+E+P++ +++GGG I +E  +++  +GS V+++   +  +   D ++  V  + ++ +   L   T +  +   E+G+  ++  G++  A     D VL A GR P
Sbjct:   39 STLGGVCLNVGCIPSKALLHVSKVIEEAKAMADHG-VVFGEPQTDINKIRIWKEKVVNQLTGGLSGMAKMRNVTVVNGYGKFTGPNSILV-EGEGESTVVNFDNAIVAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPEKLLIMGGGIIGLEMGTVYHSLGSKVEVVEMFDQVIPAADKDIVKVYTKRIKDK-FKLMLETKVTAVEAKEDGI-YVSMEGKKAPAEAERYDAVLVAIGRVP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_HAHCH (Soluble pyridine nucleotide transhydrogenase OS=Hahella chejuensis (strain KCTC 2396) GN=sthA PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.280e-14
Identity = 66/239 (27.62%), Postives = 105/239 (43.93%), Query Frame = 1
Query:    4 SAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQ-LDGTKLSYSAKHILIATGSRAQRAPIPGQELAIT------SDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            S  GG C   G +P K L +      +      +  ++ E   F + ++LQ     I +   I  +  +   V LY G    +  N +EV   L+G ++ Y  K I+IATGSR    P   +++  T      SD  L L   P+  ++ G G I  E+ASI+ G+G  VDL+   E  L   D E+   ++ +L   G+ +        ++  + GV      G+ I AD  L+  GR
Sbjct:   38 SQVGGNCTHMGTIPSKALRHAVKQIIQFNTNTMFR-DIGEPRWFSFPRVLQNAERVIGKQVKIRTQFYARNRVDLYRGRASFIDENRIEVRGGLNGKEVLYG-KQIVIATGSR----PYLPEDVDFTHRRIYNSDSILKLSHTPRTLIIYGAGVIGCEYASIFVGLGVKVDLINPGERLLSFLDGEISDALSYHLRDNGVLVRHNEQYDSVVGDDHGVVLTMKSGKRIRADAFLWCNGR 270          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_BUCAI (Dihydrolipoyl dehydrogenase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=lpdA PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.672e-14
Identity = 63/237 (26.58%), Postives = 117/237 (49.37%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDH----GEEIVADVVLFATGRAP 705
            GG C+  GC+P K L++ A    E ++    G   + K D D KK+   K   + +L      +     +++++G         + VT  +  K +    + +IATGS+  + P IP  ++ I  S +ALSL+++P   +++G G I +E A+I+  +GS VD++ R    L   D+++ ++  +++  +  NL   T I ++   ++ +     H     + I+ D VL A GR P
Sbjct:   42 GGVCLNVGCIPSKTLLHIAKVIKEAKELHKTGVSFN-KPDIDIKKIKNWKQHIVNKLTDGLSSMRKKRKIRIFQGHAIFETDKSLCVTNTED-KFTIFFDNAIIATGSKPIKIPSIPHDDIRIWDSTDALSLKKIPNNFLIIGSGIIGLEMATIYSALGSKVDIIDRFNHFLPVIDEDISSIYKKSI-NQQFNLMLNTHIDKVEVKKDALIVDMIHENIPKKNILYDAVLVAIGRTP 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_ENTAG (Mercuric reductase OS=Enterobacter agglomerans GN=merA PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.184e-14
Identity = 67/235 (28.51%), Postives = 107/235 (45.53%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRL-NGIYKRLLS-NAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAI--TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCV  GCVP KI++  A       ++   G           ++LL ++   +  L +  Y+ +L  N+ + +  GE +      + V+  +G +        L+ATG+     PIPG + +   TS EAL+ + +P+R  V+G   +A+E A  +  +GS V  L R  L  R  D  +   V       GI +   T   ++   +      T HG E+ AD +L ATGR P
Sbjct:  133 GGTCVNVGCVPSKIMIRVAHIAHLRRESPFDGGMPPTSPTILRERLLAQQQARVEELRHAKYEGILDGNSAITVLHGEARFKDDQSLIVSLNEGGERVVMFDRCLVATGASPAVPPIPGLKESPYWTSTEALASDTIPERLAVIGSSVVALELAQAFARLGSKVTALARNTLFFRE-DPAIGEAVTAAFRAEGIEVLEHTQASQVAHMDGEFVLTTTHG-ELRADKLLVATGRTP 365          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_PSEMY (Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas mendocina (strain ymp) GN=sthA PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.354e-14
Identity = 67/237 (28.27%), Postives = 106/237 (44.73%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAG------VKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR-APIPGQELAI-TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +      +      +  ++ E   F +  +L K  +++     I K++ S  G      +  Y G         VEV  L+G      AK I+IATGSR  R A +  +   I  SD  LSL   P+R ++ G G I  E+ASI+ G+G  VDL+  ++  L   D E+   ++ +L    + +      + +   E GV      G++I AD +L+  GR
Sbjct:   41 GGNCTHLGTIPSKALRHSVRQIMQFNTNPMFR-QIGEPRWFSFPDVL-KSAEKV-----IAKQVTSRTGYYARNRIDTYFGTASFADEQTVEVVCLNGVVEKLVAKQIVIATGSRPYRPADVDFRHPRIYDSDTILSLGHTPRRLIIYGAGVIGCEYASIFSGLGVLVDLIDNRDQLLSFLDSEISDALSYHLRNNNVLIRHNEEYERIEGVENGVVLHLKSGKKIKADALLWCNGR 270          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
STHA_YERE83.369e-1525.97Soluble pyridine nucleotide transhydrogenase OS=Ye... [more]
STHA_PHOLL3.369e-1527.98Soluble pyridine nucleotide transhydrogenase OS=Ph... [more]
MERA_THIFE3.369e-1530.42Mercuric reductase OS=Thiobacillus ferrooxidans GN... [more]
STHA_HAMD54.400e-1528.27Soluble pyridine nucleotide transhydrogenase OS=Ha... [more]
STHA_MARMS9.802e-1528.09Soluble pyridine nucleotide transhydrogenase OS=Ma... [more]
DLDH_VIBPA9.802e-1526.14Dihydrolipoyl dehydrogenase OS=Vibrio parahaemolyt... [more]
STHA_HAHCH1.280e-1427.62Soluble pyridine nucleotide transhydrogenase OS=Ha... [more]
DLDH_BUCAI1.672e-1426.58Dihydrolipoyl dehydrogenase OS=Buchnera aphidicola... [more]
MERA_ENTAG2.184e-1428.51Mercuric reductase OS=Enterobacter agglomerans GN=... [more]
STHA_PSEMY6.354e-1428.27Soluble pyridine nucleotide transhydrogenase OS=Ps... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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