CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH2_HALMA (Dihydrolipoyl dehydrogenase 2 OS=Haloarcula marismortui GN=lpdA2 PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.081e-21
Identity = 72/243 (29.63%), Postives = 124/243 (51.03%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVT---QLDGTKLSYSAKHILIATGSRAQRAP---IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIV----ADVVLFATGRAP 705
            A GGTC+  GC+P K L+  +    +   A S G      +D     + + K   + RL    + L  NAGV L EG  + V    V V    +  G++ S S +H ++ATGSR    P     G+ + ++S +AL+LE +P++ +V+G GYI +E ++++  +G+ V ++   +  L G++D++  VV    E  GI+ +         +++EG++  T   +E+V    A+  L A GR P
Sbjct:   42 AYGGTCLNHGCIPSKALISASDVAHDARQAESMGVFADPAVDM--AGMTEWKDGVVTRLTRGVESLCKNAGVNLVEGTAEFVDDGTVRVAHGGEGQGSE-SLSFEHAIVATGSRPMAVPGFEFDGEHI-LSSKDALALESVPEKLLVVGAGYIGMELSTVFAKLGAEVTVVEMLDDVLPGYEDDIATVVRDRAEELGIDFNFGEAADNWEETDEGIRVQTVDEDEVVTEYNAEKCLVAVGREP 280          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_CHLPN (Dihydrolipoyl dehydrogenase OS=Chlamydia pneumoniae GN=lpdA PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.844e-21
Identity = 68/230 (29.57%), Postives = 118/230 (51.30%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GGTC+ RGC+P K L+ GA+    ++ A  +G  V +    D+  + ++K   +  +    + L+ +  + + +G G +V   EV+V   D T +   A HI++ATGS  +  P +P     ++S   L LE LPK+  ++GGG I  EFAS++  +G  + ++   +  L   + E+   V      +GI +  + +I  + +S+  V RIT + +    D VL A GR
Sbjct:   39 GGTCLNRGCIPSKALIAGANVVSHIKHAEQFGIHV-DGYTIDYPAMAKRKNTVVQGIRQGLEGLIRSNKITVLKGTGSLVSSTEVKVIGQDTTIIK--ANHIILATGSEPRPFPGVPFSSRILSSTGILELEVLPKKLAIIGGGVIGCEFASLFHTLGVEITVIEALDHILAVNNKEVSQTVTNKFTKQGIRILTKASISAIEESQNQV-RITVNDQVEEFDYVLVAIGR 264          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_PSEFL (Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3)

HSP 1 Score: 102.449 bits (254), Expect = 2.409e-21
Identity = 73/239 (30.54%), Postives = 123/239 (51.46%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELEDA-RSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSR-AQRAPIP-GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGIN--LHPRTTIKELIKSEEGVKRITDHGEE-IVADVVLFATGRAP 705
            A GGTC+  GC+P K L+  +    E ++A + +G E  + +  D   ++ +K + +  L G    L    GV  +EG GK++   +VEVT LDG      A++++IA+GSR  +  P P   ++ + S  AL  + +PK+  V+G G I +E  S+W  +G+ V +L   +  L   D+++     + L  +G+N  L  R T  E+ K +  V     +GE+    D ++ A GR P
Sbjct:   44 ALGGTCLNVGCIPSKALLDSSYKYHEAKEAFKVHGIEA-KGVTIDVPAMVARKANIVKNLTGGIATLFKANGVTSFEGHGKLLANKQVEVTGLDGKTQVLEAENVIIASGSRPVEIPPAPLSDDIIVDSTGALEFQAVPKKLGVIGAGVIGLELGSVWARLGAEVTVLEALDKFLPAADEQIAKEALKVLTKQGLNIRLGARVTASEVKKKQVTVTFTDANGEQKETFDKLIVAVGRRP 281          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH3_HALMA (Dihydrolipoyl dehydrogenase 3 OS=Haloarcula marismortui GN=lpdA3 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.409e-21
Identity = 68/243 (27.98%), Postives = 119/243 (48.97%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAK--HILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTI--------KELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GG C+ RGC+P K L++G+    E   A   G      +  D  +++  K   + +L    ++L + AGV L +G  +    N+V +      + S S K  + +IATGSR    P  G   E  ++SD AL+ + +P   V++G GYI +E A+++  +GS V ++   E  L  +++++ ++V +  E  G++ H   T         K ++ +    +   D   E+ AD +L A GR P
Sbjct:   44 GGACLNRGCIPSKALIHGSKLASEAGQAEELGIYADPTVALD--EMINWKDGVVDQLTSGIEQLCTAAGVNLLKGTAEFADENKVRIIHQGEGQGSESLKFENCIIATGSRPIEIPGFGFEDERIVSSDGALNFDTVPDELVIVGAGYIGMELATVYSRLGSDVSVIEMLEQALPSYEEDIASIVRKRAERLGVDFHFGYTADSWAASDGKAVLTAVPADEAAHDSDIELTADRILVAVGRRP 284          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH1_BACSU (Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=pdhD PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.409e-21
Identity = 73/236 (30.93%), Postives = 117/236 (49.58%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRG-INLHPRTTIKELIKSEEGVKRITD-HGEE--IVADVVLFATGRAP 705
            GG C+  GC+P K L+         + +   G    E +  D+ K+ + K   + +L G    LL    V + +GE   V  N V V   + +  +Y+ K+ +IATGSR    P     E  + S  AL+L+E+PK+ VV+GGGYI  E  + +   G+ + +L   +  L GF+ +M ++V R L+ +G + +H     K + +  +GV    +  GEE  + AD VL   GR P
Sbjct:   44 GGVCLNVGCIPSKALINAGHRYENAKHSDDMGITA-ENVTVDFTKVQEWKASVVNKLTGGVAGLLKGNKVDVVKGEAYFVDSNSVRVMD-ENSAQTYTFKNAIIATGSRPIELPNFKYSERVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQMSSLVTRRLKKKGNVEIHTNAMAKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRRP 277          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH2_PSEAE (Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 4.109e-21
Identity = 73/239 (30.54%), Postives = 123/239 (51.46%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELEDA-RSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSR-AQRAPIP-GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGIN--LHPRTTIKELIKSEEGVKRITDHGEE-IVADVVLFATGRAP 705
            A GGTC+  GC+P K L+  +    E ++A + +G E  + +  D   ++ +K + +  L G    L    GV  +EG GK++   +VEVT LDG      A++++IA+GSR  +  P P   ++ + S  AL  + +PK+  V+G G I +E  S+W  +G+ V +L   +  L   D+++     + L  +G+N  L  R T  E+ K +  V     +GE+    D ++ A GR P
Sbjct:   44 ALGGTCLNVGCIPSKALLDSSYKYHEAKEAFKVHGIEA-KGVTIDVPAMVARKANIVKNLTGGIATLFKANGVTSFEGHGKLLANKQVEVTGLDGKTQVLEAENVIIASGSRPVEIPPAPLTDDIIVDSTGALEFQAVPKKLGVIGAGVIGLELGSVWARLGAEVTVLEALDKFLPAADEQIAKEALKVLTKQGLNIRLGARVTASEVKKKQVTVTFTDANGEQKETFDKLIVAVGRRP 281          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH3_PSEPU (Dihydrolipoyl dehydrogenase 3 OS=Pseudomonas putida GN=lpd3 PE=1 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.195e-20
Identity = 75/247 (30.36%), Postives = 122/247 (49.39%), Query Frame = 1
Query:    4 SAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKID----FDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITD-----HGEEIVADVVLFATGRAP 705
            S  GGTC+  GC+P K L++ +    EL +A S     H  I+     +  +++++K + +  L    + L     V   +G G++ G  +V V   DG++ +  AK I+IATGS  +  P+PG     +  I S  ALSL ++PK  VV+G G I +E  S+WR +GS V ++   +    G D E    + + L  +G+     + + +   S +GV  + +       E + AD VL A GR P
Sbjct:   36 STLGGTCLNVGCMPSKALLHAS----ELYEAASGDEFAHLGIEVKPTLNLAQMMKQKDESVTGLTKGIEYLFRKNKVDWIKGWGRLDGVGKVVVKAEDGSETALQAKDIVIATGS--EPTPLPGVTIDNQRIIDSTGALSLPQVPKHLVVIGAGVIGLELGSVWRRLGSQVTVIEYLDRICPGTDTETAKTLQKALAKQGMVFKLGSKVTQATASADGVSLVLEPAAGGTAESLQADYVLVAIGRRP 276          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH3_PSEAE (Dihydrolipoyl dehydrogenase 3 OS=Pseudomonas aeruginosa GN=lpd3 PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.561e-20
Identity = 76/245 (31.02%), Postives = 115/245 (46.94%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILV-----YGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHG-----EEIVADVVLFATGRAP 705
            GGTC+  GC+P K L+     Y A+ GGE       G  V  ++D    +++++K + +  L    + L     V+  +G  ++ G   V V   DG      A+ I+IATGS  + AP+PG     +  + S  AL L E+P+  VV+G G I +E  S+WR +G+ V +L   E    G D E    + R L  +G+     T +      E+GV+           E + AD VL A GR P
Sbjct:   40 GGTCLNVGCMPSKALLHASELYAAASGGEFA---RLGIRVSPELDL--AQMMKQKDESVAALTRGVEFLFRKHKVQWIKGWARLQGEGRVGVALADGGHAQLEARDIVIATGS--EPAPLPGVPVDNQRILDSTGALELVEVPRHLVVIGAGVIGLELGSVWRRLGAQVTVLEYLERICPGLDGETARTLQRALTRQGMRFRLGTRVVAARSGEQGVELDLQPAAGGATESLQADYVLVAIGRRP 277          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_TRYBB (Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei brucei PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.039e-20
Identity = 71/243 (29.22%), Postives = 116/243 (47.74%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELE-DARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQE-LAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEG-------VKRITDHGEEIVADVVLFATGRAP 705
            A GGTC+  GC+P K L++      +   +   YG      +  D  K+ Q+K   +  L    + LL    V  Y+GE   V PN + V  +DG   +  AK+ +IATGS     P +P  E + ++S  AL+L+++PK+ VV+GGG I +E  S+W  +GS V ++          D ++   +   L+  G +  P T I+ +  +  G       V++     E +  D +L + GR P
Sbjct:   45 ALGGTCLNVGCIPSKALLHATHMYHDAHANFERYGLMGGAGVTMDVAKMQQQKEKSVNGLTSGVEYLLKKNKVTYYKGEAGFVTPNTLNVKGIDGKDEAIEAKNTIIATGSEPTALPFLPFDEKVVLSSTGALALQQVPKKMVVIGGGVIGLELGSVWARLGSDVTVVEFAPRCAPTLDSDVTDALVGALKRNGEDEVPMTGIEGVNGTNNGSIALTLEVEQAGGQAETLHCDALLVSVGRRP 287          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_THESC (Dihydrolipoyl dehydrogenase OS=Thermus scotoductus GN=lpd PE=1 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 2.039e-20
Identity = 76/238 (31.93%), Postives = 113/238 (47.48%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAITSDEALSLEE-LPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHG-----EEIVADVVLFATGRAP 705
            GG C+  GC+P K L++ A     L+ A  +G +   K + D KKL   +   + +L G    LL    V+L  G  +  GP E+EV        +Y A+  +IATGS        P  E    S  AL +EE +PKR +V+GGG + +E   I+  +GS V L+      L   D E  A++ + LE  G+ +   T      K ++G+  + +       EEIV D +L A GR P
Sbjct:   38 GGVCLNVGCIPTKALLHAAETVHHLKGAEGFGLKA--KPELDLKKLGAWRDGVVKKLTGGVAGLLKGNKVELLRGFARFKGPREIEVNGE-----TYGAQSFIIATGSEPMPLKGFPFGEDVWDSTRALRVEEGIPKRLLVIGGGAVGLELGQIYHRLGSEVTLIEYMPEILPAGDRETAALLRKALEKEGLKVRTGTKAVGYEKKQDGLHVLLEAAQGGSQEEIVVDKILVAVGRRP 268          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
DLDH2_HALMA1.081e-2129.63Dihydrolipoyl dehydrogenase 2 OS=Haloarcula marism... [more]
DLDH_CHLPN1.844e-2129.57Dihydrolipoyl dehydrogenase OS=Chlamydia pneumonia... [more]
DLDH_PSEFL2.409e-2130.54Dihydrolipoyl dehydrogenase OS=Pseudomonas fluores... [more]
DLDH3_HALMA2.409e-2127.98Dihydrolipoyl dehydrogenase 3 OS=Haloarcula marism... [more]
DLDH1_BACSU2.409e-2130.93Dihydrolipoyl dehydrogenase OS=Bacillus subtilis G... [more]
DLDH2_PSEAE4.109e-2130.54Dihydrolipoamide dehydrogenase OS=Pseudomonas aeru... [more]
DLDH3_PSEPU1.195e-2030.36Dihydrolipoyl dehydrogenase 3 OS=Pseudomonas putid... [more]
DLDH3_PSEAE1.561e-2031.02Dihydrolipoyl dehydrogenase 3 OS=Pseudomonas aerug... [more]
DLDH_TRYBB2.039e-2029.22Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei ... [more]
DLDH_THESC2.039e-2031.93Dihydrolipoyl dehydrogenase OS=Thermus scotoductus... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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